Article
Medicine, Research & Experimental
Tao Liu, Yu Yang, Zhe Xie, Qingya Luo, Dan Yang, Xiaoyi Liu, Hongyan Zhao, Qinglv Wei, Yi Liu, Lanfang Li, Yuya Wang, Fang Wang, Jianhua Yu, Jing Xu, Jia Yu, Ping Yi
Summary: RBPs, particularly QKI5, play a tumor-suppressive role in ovarian cancer by negatively regulating the expression of TAZ and its downstream targets, such as CTGF and CYR61. QKI5 can inhibit the growth and metastasis of OC cells in vitro and in vivo, indicating its potential as a therapeutic target for OC.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Oncology
Masao Saitoh
Summary: The epithelial-mesenchymal transition (EMT) is a process where epithelial cells transform into mesenchymal cells, which is associated with invasion, metastasis, and resistance to therapy in cancer. Transcription factors, such as ZEB1/ZEB2, SNAIL, and TWIST, play important roles in driving the EMT process. This review focuses on understanding the molecular mechanisms that sustain high levels of these transcription factors in aggressive carcinomas and their upregulation by transforming growth factor-β during cancer progression.
SEMINARS IN CANCER BIOLOGY
(2023)
Article
Immunology
Avishay Dolitzky, Guy Shapira, Sharon Grisaru-Tal, Inbal Hazut, Shmulik Avlas, Yaara Gordon, Micahl Itan, Noam Shomron, Ariel Munitz
Summary: Eosinophils are multifunctional leukocytes involved in regulating tissue homeostasis, host defense, and cancer responses. They can respond to both Type 1 and Type 2 inflammatory stimuli based on recent RNA sequencing data, exhibiting distinct transcription profiles. These findings suggest that eosinophils exhibit heterogeneity and transcriptional differences between Type 1 and Type 2 cells, influencing their diverse activities in various disease settings.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Stefanie Meyer, Diana Handke, Anja Mueller, Katharina Biehl, Markus Kreuz, Juergen Bukur, Ulrike Koehl, Maria-Filothei Lazaridou, Mark Berneburg, Andre Steven, Chiara Massa, Barbara Seliger
Summary: Limited knowledge exists about the molecular processes controlling HLA class II APM components in melanoma. This study found heterogeneous basal expression of HLA class II antigens and components in melanoma cells is caused by distinct molecular mechanisms and has clinical relevance.
Article
Cell Biology
Atif Zafar, Hang Pong Ng, E. Ricky Chan, Sally L. Dunwoodie, Ganapati H. Mahabeleshwar
Summary: In this study, it was found that CITED2 is an essential negative regulator of pro-inflammatory macrophage activation and inflammatory disease pathogenesis. Myeloid-CITED2 deficiency significantly elevates HFD-induced expansion of adipose tissue volume, obesity, glucose intolerance, and insulin resistance. CITED2 promotes BCL6 expression by restraining STAT5 activation in macrophages, thereby limiting inflammation, preventing obesity, and insulin resistance.
Review
Immunology
Chih-Chun Lee, Chia-Hsuan Tsai, Chih-Hao Chen, Yuan-Chieh Yeh, Wen-Hung Chung, Chun-Bing Chen
Summary: Keloid is a unique type of disfiguring pathological scarring in human skin that is characterized by excessive collagen deposition. Immune cell infiltration is a common feature of normal and pathological tissue repair, but the immunopathological mechanisms of keloid remain unclear. Recent studies have highlighted the crucial role of innate and adaptive immunity in regulating the abnormal behavior of keloid fibroblasts. Novel therapeutics targeting immune pathways have shown varying efficacy in restoring regulation of the immune microenvironment in keloid.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Hematology
Yunfeng Liu, Mouli Pal, Weili Bao, Patricia A. Shi, Cheryl A. Lobo, Xiuli An, Deepa Manwani, Hui Zhong, Karina Yazdanbakhsh
Summary: Patients with sickle cell disease exhibit intravascular hemolysis-associated vascular injury and tissue damage. Classical monocytes in these patients show upregulation of interferon-alpha, which is correlated with total plasma heme levels. This hemolysis-induced response may lead to enhanced numbers of monocytes and macrophages, as well as increased expression of Fc receptor CD64, ultimately contributing to alloantibody-mediated erythrophagocytosis in SCD.
Article
Dentistry, Oral Surgery & Medicine
D. Wu, Y. Weng, Y. Feng, B. Liang, H. Wang, L. Li, Z. Wang
Summary: In periodontitis, Trem1 regulates polarization of M1 macrophages through the STAT3/HIF-1α signaling pathway. Studies suggest that targeting Trem1 could be a potential strategy for the treatment of periodontitis by modulating macrophage polarization.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Cell Biology
Xue-Hua Du, Shao-Bo Ke, Xin-Yi Liang, Jie Gao, Xiao-Xiao Xie, Lin-Zhi Qi, Xue-Yi Liu, Guo-Yuan Xu, Xiao-Dong Zhang, Run-Lei Du, Shang-Ze Li
Summary: Our study demonstrates that USP14 functions as a deubiquitinase that interacts and stabilizes JNK, promoting MAPK/JNK signaling and colorectal carcinogenesis. Increased expression of USP14 is associated with elevated levels of JNK protein and downstream gene expression in colorectal cancer patients. Inhibition of USP14 reduces cancer cell proliferation and tumorigenesis by downregulating the activation of the MAPK/JNK pathway.
CELL DEATH & DISEASE
(2023)
Article
Biology
Michael C. Kiritsy, Laurisa M. Ankley, Justin Trombley, Gabrielle P. Huizinga, Audrey E. Lord, Pontus Orning, Roland Elling, Katherine A. Fitzgerald, Andrew J. Olive
Summary: Two parallel pathways, involving GSK3β and MED16, control the expression of MHCII induced by IFNγ, and are essential for CD4(+) T cell activation.
Article
Biochemistry & Molecular Biology
Chintogtokh Baatarjav, Takanori Komada, Tadayoshi Karasawa, Naoya Yamada, Ariunaa Sampilvanjil, Takayoshi Matsumura, Masafumi Takahashi
Summary: This study reveals the immunoregulatory function of macrophage pyroptosis in rhabdomyolysis-induced acute kidney injury. Lack of AIM2 leads to macrophage accumulation, delayed functional recovery, and fibrosis. Aim2-deficient macrophages escape from pyroptotic elimination and engage in aggravated inflammatory responses.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Physiology
Byeong J. Chun, Surya P. Aryal, Peter Varughese, Bin Sun, Joshua A. Bruno, Chris I. Richards, Adam D. Bachstetter, Peter M. Kekenes-Huskey
Summary: ATP and its metabolites activate purinergic receptors to drive microglia migration and cytokine production. Our computational model provides insights into how different characteristics of calcium waveforms influence microglial function. We also identify CD39 metabolism as a regulator of purinergic receptor-induced calcium responses, and predict calcium transients and cell membrane displacements in a BV2 microglia cell line.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Microbiology
Na Liu, Fanli Meng, Chengming Tian
Summary: In this study, the mutants of Colletotrichum gloeosporioides lacking hypothetical sensors of plant surface signals, Delta CgMsb2 and Delta CgMsb2Sho1, were examined. The mutations adversely affected conidial size, sporulation, and growth. Transcriptional changes were observed for a large number of genes, especially those involved in hydrolases, ABC transporters, and MAPKs. The encoded MAPKs participate in signal transduction and metabolic regulation. These findings provide new insights into the regulation of C. gloeosporioides's development and infection.
Article
Biochemistry & Molecular Biology
Luciana Canonero, Constanza Pautasso, Fiorella Galello, Lorena Sigaut, Lia Pietrasanta, Javier Arroyo, Mariana Bermudez-Moretti, Paula Portela, Silvia Rossi
Summary: This study explores the mechanism of the response of the cAMP-PKA pathway to heat shock and thermal adaptation in yeast. The results demonstrate that heat shock leads to the assembly of TPK1 mRNA in cytoplasmic foci, and the protein level of Tpk1 significantly increases during the recovery period. The expression of TPK1 upon heat shock is dependent on the Wsc3 sensor and the CWI signaling pathway. This study reveals the specificity regulation of cAMP-PKA signaling by thermal stress.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Peter Androvic, Martina Schifferer, Katrin Perez Anderson, Ludovico Cantuti-Castelvetri, Hanyi Jiang, Hao Ji, Lu Liu, Garyfallia Gouna, Stefan A. Berghoff, Simon Besson-Girard, Johanna Knoferle, Mikael Simons, Ozgun Gokce
Summary: To understand the complexity of cellular function, the authors developed a method to combine spatially-resolved gene expression with ultrastructural morphology of single cells. They applied this method to study glial cells and T-cells in a demyelinating brain injury model in mice. The results revealed specific cell populations and correlations between gene expression and ultrastructural features in microglia.
NATURE COMMUNICATIONS
(2023)
Editorial Material
Physiology
Kevin S. Harrod
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2015)
Article
Urology & Nephrology
Ephraim L. Tsalik, Laurel K. Willig, Brandon J. Rice, Jennifer C. van Velkinburgh, Robert P. Mohney, Jonathan E. McDunn, Darrell L. Dinwiddie, Neil A. Miller, Eric S. Mayer, Seth W. Glickman, Anja K. Jaehne, Robert H. Glew, Mohan L. Sopori, Ronny M. Otero, Kevin S. Harrod, Charles B. Cairns, Vance G. Fowler, Emanuel P. Rivers, Christopher W. Woods, Stephen F. Kingsmore, Raymond J. Langley
KIDNEY INTERNATIONAL
(2015)
Article
Biochemistry & Molecular Biology
Yohannes A. Mebratu, Jennifer Tipper, Hitendra S. Chand, Stephanie Walton, Kevin S. Harrod, Yohannes Tesfaigzi
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Ritesh K. Srivastava, Changzhao Li, Aftab Ahmad, Onika Abrams, Marina S. Gorbatyuk, Kevin S. Harrod, Ronald C. Wek, Farrukh Afaq, Mohammad Athar
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2016)
Article
Toxicology
Scott W. Burchiel, Fredine T. Lauer, Debra MacKenzie, Shea McClain, Philip J. Kuehl, Jacob D. McDonald, Kevin S. Harrod
INHALATION TOXICOLOGY
(2016)
Article
Pharmacology & Pharmacy
Ritesh K. Srivastava, Changzhao Li, Yong Wang, Zhiping Weng, Craig A. Elmets, Kevin S. Harrod, Jessy S. Deshane, Mohammad Athar
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2016)
Article
Multidisciplinary Sciences
Niroop Kaza, S. Vamsee Raju, Joan M. Cadillac, John A. Trombley, Lawrence Rasmussen, Liping Tang, Erik Dohm, Kevin S. Harrod, Steven M. Rowe
Article
Respiratory System
Akshay Sood, Nour A. Assad, Peter J. Barnes, Andrew Churg, Stephen B. Gordon, Kevin S. Harrod, Hammad Irshad, Om P. Kurmi, William J. Martin, Paula Meek, Kevin Mortimer, Curtis W. Noonan, Rogelio Perez-Padilla, Kirk R. Smith, Yohannes Tesfaigzi, Tony Ward, John Balmes
EUROPEAN RESPIRATORY JOURNAL
(2018)
Article
Multidisciplinary Sciences
Jianguo Zhuang, Peng Gao, Zemmie Pollock, Kevin S. Harrod, Fadi Xu
Editorial Material
Biochemistry & Molecular Biology
Erin N. Yepsen, Kevin S. Harrod
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Immunology
R. Glenn King, Aaron Silva-Sanchez, Jessica N. Peel, Davide Botta, Alexandria M. Dickson, Amelia K. Pinto, Selene Meza-Perez, S. Rameeza Allie, Michael D. Schultz, Mingyong Liu, John E. Bradley, Shihong Qiu, Guang Yang, Fen Zhou, Esther Zumaquero, Thomas S. Simpler, Betty Mousseau, John T. Killian, Brittany Dean, Qiao Shang, Jennifer L. Tipper, Christopher A. Risley, Kevin S. Harrod, Tsungwei Feng, Young Lee, Bethlehem Shiberu, Vyjayanthi Krishnan, Isabelle Peguillet, Jianfeng Zhang, Todd J. Green, Troy D. Randall, John J. Suschak, Bertrand Georges, James D. Brien, Frances E. Lund, M. Scot Roberts
Summary: The study found that a single intranasal dose of AdCOVID vaccine can generate a strong and focused immune response against RBD, including mucosal IgA in the respiratory tract, serum neutralizing antibodies, and CD4(+) and CD8(+) T cells with a T(h)1-like cytokine expression profile. Additionally, a single AdCOVID dose can result in immunity sustained for over six months and completely protect K18-hACE2 mice from lethal SARS-CoV-2 challenge, preventing weight loss and mortality, indicating that AdCOVID promotes concomitant systemic and mucosal immunity and serves as a promising vaccine candidate.
Article
Medicine, Research & Experimental
Erin Y. Earnhardt, Jennifer L. Tipper, Adonis D'Mello, Ming -Yuan Jian, Elijah S. Conway, James A. Mobley, Carlos J. Orihuela, Herve Tettelin, Kevin S. Harrod
Summary: Influenza A virus (IAV) infection disrupts airway homeostasis and decreases CFTR function, leading to airway pathophysiology resembling cystic fibrosis. By using human airway organotypic cultures, this study investigates how IAV alters the airway microenvironment to increase susceptibility to secondary Streptococcus pneumoniae (Spn) infection. The researchers found that IAV-induced CFTR dysfunction and airway surface liquid acidification play a central role in increasing susceptibility to Spn, and IAV also induces transcriptional and proteomic changes in the airway epithelium, affecting host defense pathways and epithelial function.
Article
Medicine, Research & Experimental
Joselyn Rojas-Quintero, Xiaoyun Wang, Jennifer Tipper, Patrick R. Burkett, Joaquin Zuniga, Amit R. Ashtekar, Francesca Polverino, Amit Rout, Ilyas Yambayev, Carmen Hernandez, Luis Jimenez, Gustavo Ramirez, Kevin S. Harrod, Caroline A. Owen
Article
Medicine, Research & Experimental
Jeffrey D. Brand, Ahmed Lazrak, John E. Trombley, Ren-Jay Shei, A. Timothy Adewale, Jennifer L. Tipper, Zhihong Yu, Amit R. Ashtekar, Steven M. Rowe, Sadis Matalon, Kevin S. Harrod
Review
Physiology
James David Londino, Ahmed Lazrak, James F. Collawn, Zsuzsanna Bebok, Kevin S. Harrod, Sadis Matalon
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2017)