Article
Immunology
Rowayna Shouib, Gary Eitzen
Summary: Airway epithelial cells can respond to pathogens and stimulants through cytokine and chemokine secretion, but excessive secretion can cause chronic inflammation and pulmonary disorders. We identified Cdc42 as an important signaling molecule that regulates cytokine production and release. Inhibiting or silencing Cdc42 affected the gene expression and secretion of specific cytokines, particularly IL-8 trafficking and secretion.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Physiology
Michael J. O'Sullivan, Jennifer A. Mitchel, Chimwemwe Mwase, Maureen McGill, Phyllis Kanki, Jin-Ah Park
Summary: The ongoing COVID-19 pandemic poses a continuous threat to public health, and researchers need a better understanding of the cellular mechanisms of SARS-CoV-2 infection. It has been shown that well-differentiated human bronchial epithelial cells maintained in an air-liquid interface are a reliable experimental system for studying COVID-19 infection in vitro.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Virology
Andrew R. Connelly, Brian M. Jeong, Mackenzie E. Coden, Jacob Y. Cao, Tatiana Chirkova, Christian Rosas-Salazar, Jacqueline-Yvonne Cephus, Larry J. Anderson, Dawn C. Newcomb, Tina Hartert, Sergejs Berdnikovs
Summary: The study found that RSV infection in infancy leads to lasting metabolic reprogramming of airway epithelial cells, characterized by significant increase in glucose uptake and altered preferences for metabolism of several carbon and energy sources, with male epithelium showing the most pronounced changes.
Article
Multidisciplinary Sciences
Dennis K. Ninaber, Anne M. van der Does, Pieter S. Hiemstra
Summary: The airway epithelial cell layer is constantly exposed to inhaled substances and plays a central role in lung diseases. In vitro epithelial culture models offer a controlled environment for studying the role of epithelium in pathogenesis and therapy. This manuscript provides a robust protocol for isolating and culturing airway epithelial cells, along with characterization and various applications of these cultures.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Virology
Natasha Collinson, Natale Snape, Kenneth Beagley, Emmanuelle Fantino, Kirsten Spann
Summary: In this study, it was found that COPD patients showed significantly elevated production of IFN-beta in response to RSV and hMPV infections, without increased susceptibility to infection or intrinsic defects in IFN production. Elevated IFN-beta production was associated with reduced viral shedding only in WD-BECs infected with RSV.
Article
Pharmacology & Pharmacy
Natsumi Saito, Akiko Kikuchi, Mutsuo Yamaya, Xue Deng, Mitsuru Sugawara, Shin Takayama, Ryoichi Nagatomi, Tadashi Ishii
Summary: Kakkonto extract can reduce the production of specific pro-inflammatory cytokines in response to rhinovirus (RV) infection, suggesting its potential as an effective therapy to inhibit inflammation caused by RV and subsequent airway inflammation.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Environmental Sciences
Tahereh Rahmatinia, Majid Kermani, Mahdi Farzadkia, Ahmad Jonidi Jafari, Ali-Akbar Delbandi, Nesa Rashidi, Farzad Fanaei
Summary: The study revealed that increasing concentration and exposure time of PM2.5 particles can significantly impact cell viability and inflammatory responses. PM2.5 particles in high-traffic areas during summer had lower seasonal cytotoxicity compared to winter. Additionally, PM2.5 particles influenced the release of interleukins 8 and 6, with higher levels observed in winter samples.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
An-nan Zhang, Nan Li, Zhuo-chang Chen, Ya-li Guo, Cui-jie Tian, Dong-jun Cheng, Xue-yi Tang, Xiao-yu Zhang
Summary: Objective of this study was to investigate the effect of amygdalin, an active ingredient in bitter almonds, on TGF-beta-induced EMT in bronchial epithelial cells and elucidate the underlying mechanisms. The results showed that amygdalin treatment relieved airway wall remodeling and decreased the expressions of EMT markers. Furthermore, amygdalin inhibited TGF-beta-induced EMT through regulating the expression of HPSE.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Plant Sciences
Maria Graca Miguel, Carina Isabel da Silva, Luana Farah, Fernao Castro Braga, Ana Cristina Figueiredo
Summary: This study evaluated the effect of 20 essential oils on the release of CCL2 and TNF-alpha by LPS-stimulated THP-1 cells. Some essential oils showed toxicity to cells, while T. capitata and L. luisieri essential oils exhibited good inhibitory effects on TNF-alpha release.
Article
Toxicology
Shan Yi, Yan-ping Liu, Xun-ya Li, Xiao-yan Yuan, Ye Wang, Ying Cai, Yuan-di Lei, Lian Huang, Zhao-hui Zhang
Summary: This study showed that beryllium sulfate (BeSO4) can increase the levels of inflammatory cytokines in human bronchial epithelial cells (16HBE) and alter the expression of miRNAs. A total of 179 differentially expressed miRNAs were identified, mainly involved in transcription regulation and signaling pathways such as MAPK and VEGF. The findings provide new insights into the toxicity mechanism of beryllium exposure.
JOURNAL OF APPLIED TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Bo-Jeong Pyun, Kyuhyung Jo, Joo Young Lee, Ami Lee, Myung-A Jung, Youn-Hwan Hwang, Dong Ho Jung, Kon-Young Ji, Susanna Choi, Yun Hee Kim, Taesoo Kim
Summary: Caesalpinia sappan Linn. heartwood water extract (CSLW) has anti-inflammatory and antioxidant properties and can alleviate symptoms of allergic rhinitis (AR) by controlling the allergic inflammatory reaction of the nasal epithelium.
Article
Multidisciplinary Sciences
Natalie J. Ronaghan, Mandy Soo, Uriel Pena, Marisa Tellis, Wenming Duan, Nooshin Tabatabaei-Zavareh, Philipp Kramer, Juan Hou, Theo J. Moraes
Summary: The study investigated the role of different types of alveolar macrophages in respiratory syncytial virus (RSV) infection using an in vitro co-culture model. The results showed that M1-like macrophages decreased HBEC infection with RSV, while M0- or M2-like macrophages enhanced the infection. The study also demonstrated the potential of the in vitro co-culture model to further investigate the roles of macrophages in bronchial viral infection.
Article
Biochemistry & Molecular Biology
Tamara Gulic, Gordana Laskarin, Lana Glavan, Tanja Grubic Kezele, Herman Haller, Daniel Rukavina
Summary: The study investigated the binding of gp96 to Toll-like receptor (TLR) 4 and CD91 receptors on decidual CD1a(+) DCs in vitro, as well as the influence on the maturation status of CD1a(+) DCs. It was found that gp96 efficiently bound CD91 and TLR4 receptors, leading to the maturation of CD1a(+) DCs and significantly increasing the expression of INF-gamma and IL-15, which could potentially have detrimental effects on ongoing pregnancy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Xin-hui Yuan, Li-li Pang, Jing Yang, Yu Jin
Summary: This study found that HRV-C can infect and propagate in HBE cells, and significantly increase the secretion of various cytokines. Compared with RSV, HRV-C induces weaker cytokine expression.
Article
Immunology
Dinesh Devadoss, Shashi P. Singh, Arpan Acharya, Kieu Chinh Do, Palsamy Periyasamy, Marko Manevski, Neerad Mishra, Carmen S. Tellez, Sundaram Ramakrishnan, Steven A. Belinsky, Siddappa N. Byrareddy, Shilpa Buch, Hitendra S. Chand, Mohan Sopori
Summary: Lung epithelial cells may serve as potential HIV reservoirs contributing to respiratory comorbidities in PLWH. NHBEs express higher levels of CD4 and CXCR4 than A549 cells. In the presence of cART, HIV-1 induces latency and integration of HIV provirus in cellular DNA.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Obstetrics & Gynecology
Fionnuala Mone, Andrew Thompson, Moira C. Stewart, Ricardo Segurado, Jennifer Curry, James C. Dornan, Fionnuala M. McAuliffe, Michael D. Shields
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2020)
Article
Virology
Connor G. G. Bamford, Lindsay Broadbent, Elihu Aranday-Cortes, Mary McCabe, James McKenna, David G. Courtney, Olivier Touzelet, Ahlam Ali, Grace Roberts, Guillermo Lopez Campos, David Simpson, Conall McCaughey, Derek Fairley, Ken Mills, Ultan F. Power
Summary: This study finds that the evolution of SARS-CoV-2 is restrained in pediatric respiratory epithelial cells but still exhibits significant genetic plasticity shaped by both viral and host cell factors.
Article
Microbiology
Celine Pattaroni, Matthew Macowan, Roxanne Chatzis, Carmel Daunt, Adnan Custovic, Michael D. Shields, Ultan F. Power, Jonathan Grigg, Graham Roberts, Peter Ghazal, Jurgen Schwarze, Mindy Gore, Steve Turner, Andrew Bush, Sejal Saglani, Clare M. Lloyd, Benjamin J. Marsland
Summary: This study reveals the relationship between early post-natal microbial and immunological features in the upper airways of newborns. The results show that the oropharynx and nasal cavity are distinct ecological niches for different microbial species, and breastfeeding can influence the microbial composition and expression of immunological features. These findings are important for understanding the development trajectories of respiratory health.
Review
Pediatrics
Michael Paul Corr, Derek Fairley, James P. McKenna, Michael D. Shields, Thomas Waterfield
Summary: There is a lack of research on the diagnostic accuracy of MR-proADM in the diagnosis of invasive bacterial infections in children. Initial results suggest that MR-proADM testing alone is not effective in identifying IBI in young children. It remains unclear if MR-proADM performs differently in older children or in children with signs and symptoms of IBI.
Article
Pediatrics
Lesley Kennedy, Gillian Gallagher, Barbara Maxwell, Brigitte Bartholme, Andrew Fitzsimons, Catherine Russell, Orla Mallon, Jenny L. Hughes, Susan Beattie, Veena Vasi, Dara Bartholomew O'Donoghue, Michael David Shields
Summary: This study reports on the development, implementation, and evaluation of a safe asthma discharge care pathway (SADCP). By teaching correct inhaler technique and providing personalized asthma action plans, the pathway can effectively reduce the risk of future asthma attacks in children.
FRONTIERS IN PEDIATRICS
(2022)
Review
Pediatrics
Ahmad Kantar, Julie M. Marchant, Woo-Jung Song, Michael D. Shields, Grigorios Chatziparasidis, Angela Zacharasiewicz, Alexander Moeller, Anne B. Chang
Summary: Chronic cough is a common symptom of various respiratory and non-respiratory disorders. Managing chronic cough in children requires a detailed history taking to accurately identify the underlying cause, facilitate evaluation and treatment, and make specific diagnoses.
FRONTIERS IN PEDIATRICS
(2022)
Review
Public, Environmental & Occupational Health
Paddy McCrossan, Dara O'Donoghue, James Charles McElnay, Michael D. D. Shields
Summary: Incorrect inhaler technique and non-adherence to inhaled preventer therapy often result in poorly controlled asthma. Detecting and correcting non-adherence has been challenging, and recent evidence shows that critical errors in inhaler technique occur at home. Remote video directly observed therapy (vDOT) has emerged as a potentially useful tool for addressing non-adherence and correcting inhaler technique errors in a timely manner.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Pediatrics
Gail Davison, Josh Ruddell, Michelle Trouton, Roisin McDonald, Ben Kennedy, Vikki O'Neill, Joseph McCann, Brigitte Bartholome, Heather Steen, Mike D. Shields, Stephen Mullen
Summary: The aim of this study was to retrospectively evaluate a clinical management algorithm for acute wheezers in a UK Pediatric Emergency Department. By modifying the steroid treatment and implementing various measures, the algorithm successfully reduced steroid intolerability and costs without significantly affecting other outcomes.
PEDIATRIC PULMONOLOGY
(2023)
Review
Pediatrics
Patrick McCrossan, Orla Mallon, Michael D. Shields, Catherine Russell, Lesley Kennedy, Dara O'Donoghue
Summary: Poor inhaler technique is one reason for poorly controlled asthma in children. This scoping review explores the teaching methods used to train children to master correct inhaler technique. Various methods have been used, involving physical demonstrations and applicable to different types of inhalers.
ITALIAN JOURNAL OF PEDIATRICS
(2022)
Article
Medicine, General & Internal
Hong Guo-Parke, Dermot Linden, Sinead Weldon, Joseph C. Kidney, Clifford C. Taggart
Summary: COPD is a chronic lung disorder that affects 10% of the population worldwide and is the third leading cause of death. Respiratory viruses are a primary cause of COPD exacerbations, leading to secondary bacterial infections. COPD patients are more susceptible to viral infections, which can result in severe disease, accelerated lung function deterioration, hospitalization, and increased mortality.
MEDICINA-LITHUANIA
(2022)
Meeting Abstract
Respiratory System
Hannah Ferrin, Lauren Quinn, Niamh Galway, Michael D. Shields, Dara O'Donoghue
EUROPEAN RESPIRATORY JOURNAL
(2021)
Meeting Abstract
Medicine, General & Internal
Hannah Ferrin, Lauren Quinn, Niamh Galway, Michael D. Shields, Dara B. O'Donoghue
IRISH JOURNAL OF MEDICAL SCIENCE
(2021)
Letter
Respiratory System
Michael D. Shields, James McElnay
Article
Pediatrics
Stephen Mullen, Michael D. Shields, Dara O'Donoghue
BMJ PAEDIATRICS OPEN
(2020)
Review
Medicine, General & Internal
Michael Corr, Thomas Waterfield, Derek Fairley, James McKenna, Michael D. Shields
SYSTEMATIC REVIEWS
(2020)