Article
Surgery
Daniel R. Calabrese, Tiffany Chong, Jonathan P. Singer, Raja Rajalingam, Steven R. Hays, Jasleen Kukreja, Lorriana Leard, Jeffrey A. Golden, Lewis L. Lanier, John R. Greenland
Summary: Acute and chronic rejections limit the long-term survival after lung transplant. Pulmonary antibody-mediated rejection (AMR) is an incompletely understood driver of long-term outcomes characterized by donor-specific antibodies (DSAs), innate immune infiltration, and evidence of complement activation.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Review
Biochemistry & Molecular Biology
Antony Hoarau, Myriam Polette, Christelle Coraux
Summary: Hyaluronic acid (HA) is a major component of the extracellular matrix with anti-inflammatory and immunomodulatory functions in the lungs. The size of HA determines its pro-inflammatory or anti-inflammatory effects. The interactions between HA and hyaladherins play a role in HA activities. LMW-HA is involved in pulmonary innate immunity by activating various cells, making it a potential therapeutic target for inflammatory respiratory diseases.
Article
Immunology
Emer Ryan, Lynne Kelly, Catherine Stacey, Dean Huggard, Eimear Duff, Danielle McCollum, Ann Leonard, Gerard Boran, Dermot R. Doherty, Turlough Bolger, Eleanor J. Molloy
Summary: This study demonstrates altered cytokine profiles and endotoxin responses in children with traumatic brain injury (TBI), including mild TBI. Children with mild TBI showed decreased levels of IL-8, IL-10, IL-17A, and TNF-alpha compared to controls, along with increased levels of IFN-gamma. In severe TBI, there was no significant IL-6 response to endotoxin, but an increase in IL-17A. These findings suggest immune dysfunction following TBI, especially in mild cases.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Biochemistry & Molecular Biology
Ryota Tanida, Hironobu Tsubouchi, Shigehisa Yanagi, Yusuke Saito, Koji Toshinai, Taiga Miyazaki, Toshinari Takamura, Masamitsu Nakazato
Summary: This study investigated the role of endogenous ghrelin in LPS-induced ARDS model. The results showed that ghsr(-/-) mice had better survival rates, lower levels of inflammatory cytokines, and higher oxygenation levels. Peritoneal macrophages isolated from ghsr(-/-) mice also had lower cytokine production and oxygen consumption rate. These findings suggest that endogenous ghrelin plays a crucial role in the initiation and continuation of acute inflammatory response in ARDS by modulating macrophage activity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Immunology
Holly J. Hulsebus, Kevin M. Najarro, Rachel H. McMahan, Devin M. Boe, David J. Orlicky, Elizabeth J. Kovacs
Summary: Alcohol consumption increases the risk of infections, particularly pneumococcal pneumonia. Pneumonia patients with alcohol use require more intensive treatment and longer hospital stays. Alcohol consumption affects the immune response in the respiratory tract.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Neurosciences
Lauren E. Fritsch, Jing Ju, Erwin Kristobal Gudenschwager Basso, Eman Soliman, Swagatika Paul, Jiang Chen, Alexandra M. Kaloss, Elizabeth A. Kowalski, Taylor C. Tuhy, Rachana Deven Somaiya, Xia Wang, Irving Coy Allen, Michelle H. Theus, Alicia M. Pickrell
Summary: This study found that the cGAS-STING-mediated Type I interferon signaling pathway plays a critical role in neural tissue damage following TBI, and that mtDNA may be a possible trigger in this response.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Cell Biology
Brian Dobosh, Keivan Zandi, Diego Moncada Giraldo, Shu Ling Goh, Kathryn Musall, Milagros Aldeco, Julia LeCher, Vincent D. Giacalone, Junkai Yang, Devon J. Eddins, Manoj Bhasin, Eliver Ghosn, Vikas Sukhatme, Raymond F. Schinazi, Rabindra Tirouvanziam
Summary: This study reveals the role of lung-infiltrating monocytes in COVID-19 pathogenesis and highlights their significance as a therapeutic target. The researchers found that monocytes acquire SARS-CoV-2 from lung epithelial cells and upregulate inflammatory mediator expression and secretion. Combination therapy with baricitinib and remdesivir enhances antiviral signaling and viral clearance while reducing the secretion of proneutrophilic mediators associated with acute respiratory distress syndrome.
Article
Physiology
Elim Na, Eri Allen, Lillia A. Baird, Christine V. Odom, Filiz T. Korkmaz, Anukul T. Shenoy, Adeline M. Matschulat, Matthew R. Jones, Darrell N. Kotton, Joseph P. Mizgerd, Xaralabos Varelas, Katrina E. Traber, Lee J. Quinton
Summary: During bacterial pneumonia, alveolar epithelial cells play a critical role and LIF signaling in epithelial cells regulates the response to bacterial pneumonia. However, LIFR may have more prominent roles in other cell types, which requires further investigation.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Review
Immunology
Paulina Misiukiewicz-Stepien, Magdalena Paplinska-Goryca
Summary: Despite ongoing efforts to reduce air pollutant emissions, environmental pollution continues to increase, particularly impacting respiratory diseases due to exposure to elevated levels of airborne particles. PM10, the largest fraction of air pollutants, contains various harmful components that can disrupt natural airway defense mechanisms and promote respiratory issues. This review highlights the importance of understanding the physical features of PM10 and its impact on airway epithelial cells and obstructive pulmonary diseases.
CLINICAL IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anuradha Ray, Sagar L. Kale, Richard P. Ramonell
Summary: Despite advancements in pulmonary immunology, there is still much work to be done in understanding coordinated immune responses in the lung. The SARS-CoV-2 pandemic has shown the inadequacy of current models of host-pathogen interactions and emphasized the importance of unraveling complex biological questions for immunologists. The 64th Annual Thomas L. Petty Aspen Lung Conference focused on bridging the gap between innate and adaptive immunity in the lung, discussing disease pathogenesis, immune responses, autoimmune lung diseases, immunologic cancer treatments, and new paradigms for lung disease prevention and treatment.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Review
Immunology
Zhaojun Liu, Wei Xu
Summary: AB infection is a global public health threat and difficult to treat due to drug resistance. Studying immune therapy, such as anti-infection immunity, is necessary. Macrophages and neutrophils play important roles in the immune response to AB infection and are closely associated with lung injury. Understanding this relationship can help develop treatments and prevent lung damage.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Kenta Ikoma, Michihiro Takahama, Atsushi Kimishima, Yixi Pan, Manabu Taura, Akiyoshi Nakayama, Masayoshi Arai, Naoki Takemura, Tatsuya Saitoh
Summary: The study found that oridonin, a diterpenoid extracted from Isodon japonicus HARA, strongly suppressed particulate-induced cell death and the release of inflammatory cytokines in macrophages. Oridonin also reduced particulate-induced phagolysosomal membrane damage without affecting the phagocytosis of particulates. Furthermore, oridonin treatment markedly suppressed symptoms of silica particle-induced pneumonia by inhibiting the release of IL-1 alpha independently of NLRP3.
INTERNATIONAL IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Spela Knez, Mojca Narat, Jernej Ogorevc
Summary: Toll-like receptor 10 (TLR10) is a member of TLR family with unclear function and ligand. Its role as an immunomodulator appears to be cell type, pathogen, and TLR10 expression level-dependent. In this study, we overexpressed TLR10 in A549 lung epithelial cells and observed differential expression of TLR signaling genes activated by various TLR ligands. The overexpression of TLR10 suppressed proinflammatory cytokine expression and increased anti-inflammatory cytokine and antimicrobial peptide expression, suggesting its potential as an anti-inflammatory molecule in A549 cells.
Article
Immunology
Mangalakumari Jeyanathan, Maryam Vaseghi-Shanjani, Sam Afkhami, Jensine A. Grondin, Alisha Kang, Michael R. D'Agostino, Yushi Yao, Shreya Jain, Anna Zganiacz, Zachary Kroezen, Meera Shanmuganathan, Ramandeep Singh, Anna Dvorkin-Gheva, Philip Britz-McKibbin, Waliul I. Khan, Zhou Xing
Summary: This study demonstrates that subcutaneous BCG vaccination can induce memory alveolar macrophages (AMs) and trained immunity in the lung. The induction of memory AMs is independent of circulating monocytes and is related to the distribution of mycobacteria, alterations in the intestinal microbiome, and changes in circulating and lung metabolites.
Article
Immunology
Alice Hamilton, Raffaella Rizzo, Samuel Brod, Masahiro Ono, Mauro Perretti, Dianne Cooper, Fulvio D'Acquisto
Summary: Social isolation has a significant impact on the immune regulation of mice, enhancing their resistance to bacterial infection. Social housing and huddling behavior may be important factors in regulating the host immune response.
BRAIN BEHAVIOR AND IMMUNITY
(2022)
Editorial Material
Allergy
Stephanie Stojanovic, Celia Zubrinich, Asger Sverrild, Janine Mahoney, Eve Denton, Joy Lee, Mark Hew
CLINICAL AND EXPERIMENTAL ALLERGY
(2022)
Article
Respiratory System
Cristina Baciu, Jason Shin, Michael Hsin, Marcelo Cypel, Shaf Keshavjee, Mingyao Liu
Summary: This study aims to investigate the metabolomic and transcriptomic profiles in human lungs before and after reperfusion during lung transplantation. The results showed activation of energy metabolism and reduced antioxidative biochemicals during reperfusion. Upregulation of genes related to cytokines and inflammatory mediators were identified. Purine metabolism was found to be the most significantly enriched pathway during reperfusion. Levels of inosine and uric acid were significantly associated with intensive care unit length of stay and ventilation time, indicating the potential role of oxidative stress through purine metabolism pathway in ischaemia reperfusion injury of lung grafts.
Article
Allergy
Asger Sverrild, John Carruthers, Kavitha Garuna Murthee, Alice Moore, Robyn Elizabeth O'Hehir, Robert Puy, Mark Hew, Celia Zubrinich
Summary: This study evaluated a published algorithm based on tryptase and clinical presentation to identify appropriate individuals for further testing for IgE-mediated perioperative hypersensitivity (POH). The results showed that the algorithm was highly sensitive but less specific in identifying patients testing positive on skin testing and/or specific IgE. Cardiovascular symptoms had high sensitivity, while the combination of respiratory symptoms and increased tryptase had the highest specificity. Respiratory involvement was more common in neuromuscular blocking agent allergy, while urticaria/angioedema was more common in antibiotic allergy.
Article
Cardiac & Cardiovascular Systems
John Kenneth Peel, Shaf Keshavjee, David Naimark, Mingyao Liu, Lorenzo Del Sorbo, Marcelo Cypel, Kali Barrett, Eleanor M. Pullenayegum, Beate Sander
Summary: This study compared the institutional costs of lung transplantation before and after the availability of EVLP and determined the health-economic impact of EVLP. The study found that EVLP availability was associated with faster progression to transplantation without significant marginal cost increase.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Article
Cardiac & Cardiovascular Systems
Rayoun Ramendra, Andrew T. Sage, Jonathan Yeung, Juan C. Fernandez-Castillo, Marcelo Cuesta, Meghan Aversa, Mingyao Liu, Marcelo Cypel, Shaf Keshavjee, Tereza Martinu
Summary: This study investigates the utility of measuring total bile acids (TBA) in donor large airway bronchial wash (LABW) to predict the performance of donor lungs and recipient outcomes.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Letter
Surgery
Andrea Mariscal, Jussi Tikkanen, Lindsay Calderone, Olivia Hough, Manyin Chen, Tereza Martinu, Stephen Juvet, Marcelo Cypel, Mingyao Liu, Shaf Keshavjee
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Pharmacology & Pharmacy
Ar Kar Aung, Celia M. Zubrinich, Michelle S. Y. Goh, Benjamin Snyder, Mei Jie Tang, Cindy Y. L. Khu, Jennifer I. Lee, Linda V. Graudins
Summary: A reliable assessment tool, AQUA-12, was developed to monitor the quality of adverse drug reaction (ADR) reports in a quaternary hospital setting. The tool demonstrated excellent inter-rater reliability and identified key elements missing from reports. AQUA-12 can be used to regularly monitor the completeness of ADR reports and guide quality improvement initiatives in hospital settings.
EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY
(2023)
Review
Pharmacology & Pharmacy
Leeann Yao, Dejan Bojic, Mingyao Liu
Summary: The use of gold nanoparticles in medicine has vast clinical implications and exciting therapeutic potential. Current studies suggest that there are no major safety concerns with the use of gold nanoparticles, but more research and clinical trials are needed to further understand their safety and efficacy in clinical settings.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Review
Biotechnology & Applied Microbiology
Antti I. Nykanen, Mingyao Liu, Shaf Keshavjee
Summary: Lung transplantation is often the only option for end-stage lung disease patients. Mesenchymal stromal cells (MSC) have anti-inflammatory and protective effects, which have potential benefits in solid organ transplantation. This review discusses the experimental studies on using MSC to protect donor lungs from ischemia-reperfusion injury and alloimmune responses, as well as the studies on using MSC to prevent or treat chronic lung allograft dysfunction. The ex vivo lung perfusion (EVLP) is also mentioned as an optimal platform for MSC delivery, and the potential of genetic engineering to enhance the therapeutic potential of MSCs.
BIOENGINEERING-BASEL
(2023)
Article
Engineering, Biomedical
Yujian Liu, Kaifu Zheng, Zijie Meng, Lei Wang, Xi Liu, Baolin Guo, Jiankang He, Xiyang Tang, Mingyao Liu, Nan Ma, Xiaofei Li, Jinbo Zhao
Summary: In this study, a cell-free tissue-engineered tracheal substitute was developed using a 3D-printed polycaprolactone scaffold coated with a gelatin-methacryloyl hydrogel. Transforming growth factor-β1 (TGF-β) and stromal cell-derived factor-1α (SDF-1) were embedded in the scaffold to facilitate cell recruitment and differentiation. The tracheal substitute showed positive results in promoting the recruitment of cells and enhancing differentiation towards a chondrocyte phenotype.
Article
Biochemistry & Molecular Biology
Lei Huang, Ravi N. Vellanki, Zhiyuan Zhu, Bradly G. Wouters, Shaf Keshavjee, Mingyao Liu
Summary: Ex vivo lung perfusion (EVLP) has improved lung transplant success by evaluating marginal lungs before transplantation. To develop EVLP as a platform for donor lung reconditioning, a prolonged EVLP and new perfusates providing sufficient nutritional support are necessary. A combination of DMEM and a current EVLP perfusate (Steen solution) enhanced cell function and reduced apoptosis. A new EVLP perfusate, D05D7A solution, based on DMEM was designed and tested, showing improved cell function and higher levels of GSH and HSP70 compared to Steen solution. DMEM-based nutrient-rich perfusate could prolong EVLP and improve donor lung quality for transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Jamie E. Jeon, Lei Huang, Zhiyuan Zhu, Aaron Wong, Shaf Keshavjee, Mingyao Liu
Summary: This study investigated the effects of ischemia-reperfusion and ex vivo lung perfusion (EVLP) on gene expression in human pulmonary microvascular endothelial cells and epithelial cells. The results showed that both cell types exhibited changes in gene expressions after ischemia-reperfusion or EVLP, with the former showing upregulated pro-inflammatory genes and downregulated cell metabolism pathways, while the latter showed downregulation of cell metabolism without any inflammatory signals.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Review
Cell Biology
Yajin Zhao, Kimberly Main, Tanroop Aujla, Shaf Keshavjee, Mingyao Liu
Summary: Organ transplantation is the only treatment option for end-stage organ dysfunction, but it faces various limitations. This study reveals the mechanisms of necroptosis in organ transplantation and identifies potential targeted drugs.
Editorial Material
Respiratory System
Gregory P. Downey
Summary: The climate crisis has a wide-ranging impact on human health, and the healthcare industry itself contributes to climate change in multiple ways. To mitigate the environmental footprint, the industry should switch to more sustainable inhalers, implement carbon offset programs, invest in research and development, and raise awareness among healthcare professionals.
CANADIAN JOURNAL OF RESPIRATORY CRITICAL CARE AND SLEEP MEDICINE
(2023)
Letter
Respiratory System
Gregory P. Downey, M. Patricia Rivera, Lynn M. Schnapp, Irina Petrache, Jesse Roman, Karen J. Collishaw
ANNALS OF THE AMERICAN THORACIC SOCIETY
(2023)