期刊
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
卷 304, 期 11, 页码 R966-R979出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpregu.00595.2012
关键词
adrenergic tone; cholinergic tone; embryo and fetus; hypoxia; reptile; gene expression
类别
资金
- National Science Foundation (NSF) [IBN IOS-0845741, DGE-0808392, IBN IOS-0923300]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [0923300] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [845741] Funding Source: National Science Foundation
- EPSCoR
- Office Of The Director [0814442] Funding Source: National Science Foundation
Reptile embryos tolerate large decreases in the concentration of ambient oxygen. However, we do not fully understand the mechanisms that underlie embryonic cardiovascular short- or long-term responses to hypoxia in most species. We therefore measured cardiac growth and function in snapping turtle embryos incubated under normoxic (N21; 21% O-2) or chronic hypoxic conditions (H10; 10% O-2). We determined heart rate (f(H)) and mean arterial pressure (P-m) in acute normoxic (21% O-2) and acute hypoxic (10% O-2) conditions, as well as embryonic responses to cholinergic, adrenergic, and ganglionic pharmacological blockade. Compared with N21 embryos, chronic H10 embryos had smaller bodies and relatively larger hearts and were hypotensive, tachycardic, and following autonomic neural blockade showed reduced intrinsic f(H) at 90% of incubation. Unlike other reptile embryos, cholinergic and ganglionic receptor blockade both increased f(H). beta-Adrenergic receptor blockade with propranolol decreased f(H), and alpha-adrenergic blockade with phentolamine decreased P-m. We also measured cardiac mRNA expression. Cholinergic tone was reduced in H10 embryos, but cholinergic receptor (Chrm2) mRNA levels were unchanged. However, expression of adrenergic receptor mRNA (Adrb1, Adra1a, Adra2c) and growth factor mRNA (Igf1, Igf2, Igf2r, Pdgfb) was lowered in H10 embryos. Hypoxia altered the balance between cholinergic receptors, alpha-adrenoreceptor and beta-adrenoreceptor function, which was reflected in altered intrinsic f(H) and adrenergic receptor mRNA levels. This is the first study to link gene expression with morphological and cardioregulatory plasticity in a developing reptile embryo.
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