期刊
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
卷 298, 期 2, 页码 R378-R384出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpregu.00458.2009
关键词
detrusor contractility; urinary bladder smooth muscle; calcium-activated potassium channel opener
类别
资金
- Totman Trust for Medical Research
- National Heart, Lung, and Blood Institute Training Program in the Molecular Basis of Cardiovascular Disease [T32 HL-007944, HL-007647-19]
- National Institute of Diabetes and Digestive and Kidney Diseases [DK53832, DK-65947]
- National Heart, Lung, and Blood Institute [HL-44455, HL-77378]
Layne JJ, Nausch B, Olesen S-P, Nelson MT. BK channel activation by NS11021 decreases excitability and contractility of urinary bladder smooth muscle. Am J Physiol Regul Integr Comp Physiol 298: R378-R384, 2010. First published November 18, 2009; doi:10.1152/ajpregu.00458.2009.-Large-conductance Ca2+ activated potassium (BK) channels play an important role in regulating the function and activity of urinary bladder smooth muscle (UBSM), and the loss of BK channel function has been shown to increase UBSM excitability and contractility. However, it is not known whether activation of BK channels has the converse effect of reducing UBSM excitability and contractility. Here, we have sought to investigate this possibility by using the novel BK channel opener NS11021. NS11021 (3 mu M) caused an approximately threefold increase in both single BK channel open probability (P-o) and whole cell BK channel currents. The frequency of spontaneous action potentials in UBSM strips was reduced by NS11021 from a control value of 20.9 +/- 5.9 to 10.9 +/- 3.7 per minute. NS11021 also reduced the force of UBSM spontaneous phasic contractions by similar to 50%, and this force reduction was blocked by pretreatment with the BK channel blocker iberiotoxin. NS11021 (3 mu M) had no effect on contractions evoked by nerve stimulation. These findings indicate that activating BK channels reduces the force of UBSM spontaneous phasic contractions, principally through decreasing the frequency of spontaneous action potentials.
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