Review
Geriatrics & Gerontology
Benrong Liu, Wen-Chao Ou, Lei Fang, Chao-Wei Tian, Yujuan Xiong
Summary: Cell regulatory networks are crucial for maintaining cellular homeostasis, with MEF2A playing a key role in regulating various cellular processes. It is involved in growth, differentiation, survival, and death, and has important functions in heart development, myogenesis, and neuronal development.
Article
Endocrinology & Metabolism
Erik A. Richter
Summary: Exercise in humans greatly increases muscle glucose uptake, but the translocation of GLUT4 to the cell membrane only doubles, indicating a significant disparity. This could be due to underestimated measurements or an increase in GLUT4 intrinsic activity during exercise.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Multidisciplinary Sciences
Peter R. T. Bowman, Godfrey L. Smith, Gwyn W. Gould
Summary: Diabetes is considered one of the most urgent global public health challenges, with research focused on understanding disease mechanisms and improving interventions. A significant clinical need exists in addressing the elevated rate of cardiomyopathy in diabetic patients, a major contributing factor to premature death in this population.
Article
Biochemistry & Molecular Biology
Mostafa Samak, Andreas Kues, Diana Kaltenborn, Lina Kloesener, Matthias Mietsch, Giulia Germena, Rabea Hinkel
Summary: Cardiovascular complications are the leading cause of morbidity and mortality in diabetes, primarily due to vascular inflammation. This study investigates the molecular basis of these complications, focusing on the anti-inflammatory factors KLF2, KLF4, and MEF2D. The dysregulation of these factors in diabetes is associated with increased monocyte adhesion and migratory defects in cardiac microvascular endothelial cells. The study also reveals a direct interaction between miR-92a and these factors, highlighting miR-92a as a potential therapeutic target for diabetes-associated cardiovascular disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Malwina Lisek, Oskar Przybyszewski, Ludmila Zylinska, Feng Guo, Tomasz Boczek
Summary: The family of myocyte enhancer factor 2 (MEF2) transcription factors plays a crucial role in neuronal development and synaptic plasticity. MEF2s are involved in regulating the growth, pruning, and survival of neurons in the developing brain. They also impact learning and memory formation by controlling the number of synapses in the hippocampus. Dysfunctions in MEF2 activity have been linked to age-dependent neuronal dysfunctions and neurodegenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Benrong Liu, Lihua Pang, Yang Ji, Lei Fang, Chao Wei Tian, Jing Chen, Changnong Chen, Yun Zhong, Wen-Chao Ou, Yujuan Xiong, Shi Ming Liu
Summary: Resveratrol enhances the expression of MEF2A and the upregulation of MEF2A is required for the protective effects of resveratrol on vascular endothelial cells. Silencing MEF2A can impair the protective function of resveratrol and aggravate atherosclerotic lesion.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Mingjian Liu, Yu Liu, Xuan Li, Miao Pei, Mei Han, Feng Qi
Summary: Myofascial pain syndrome (MPS) is a chronic pain disorder characterized by myofascial trigger points (MTrPs). This study found that MEF2C is involved in the inflammatory pathogenesis of MTrPs, and suggested that DEX may serve as a potential therapeutic strategy for MPS treatment.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Food Science & Technology
Tongxi Zhou, Guanjun Song, Di Tian, Qinghua Liu, Jinhua Shen, Xinzhou Yang, Ping Zhao
Summary: Nuciferine found in lotus leaves has been shown to improve hyperlipemia and hyperglycemia. In vitro studies revealed that nuciferine stimulates the translocation of glucose transporter 4 (GLUT4) through G protein-PLC-PKC and AMPK pathways, and increases intracellular Ca2+ levels through G protein-PLC-IP3-IP3R pathway, both of which promote glucose uptake. In vivo experiments using KK-Ay mice demonstrated that nuciferine ameliorates symptoms of insulin resistance, abnormal lipid metabolism, and weight gain induced by a high-fat diet. This research highlights the potential of nuciferine in the treatment of type 2 diabetes mellitus.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Article
Immunology
Qinying Sun, Yang Xia, Hao Qin, Wei Zhang, Jun Wang, Yunye Ning, Yuchao Dong
Summary: This study reveals that MEF2 can promote M2 polarization and inhibit M1 polarization of macrophages. Overexpression of MEF2 can alleviate LPS-induced ALI. Furthermore, MEF2 regulates macrophage phenotypes through the modulation of KLF2. MiR-33 may be involved in macrophage polarization by inhibiting MEF2 expression.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Margarita Beckerman, Chava Harel, Inbal Michael, Amira Klip, Philip J. Bilan, Emily J. Gallagher, Derek LeRoith, Eli C. Lewis, Eddy Karnieli, Shulamit Levenberg
Summary: The study successfully demonstrated that engineered muscle constructs composed of GLUT4-overexpressing cells can effectively reduce and stabilize basal glucose levels in diabetic mice, suggesting a potential innovative modality for T2D therapy.
Article
Biochemistry & Molecular Biology
Shicong Zhu, Cheng Xing, Guangsen Zhang, Hongling Peng, Zhihua Wang
Summary: In this study, CC1007 was found to inhibit the proliferation of multiple myeloma cells and induce apoptosis and cell cycle arrest. Additionally, CC1007 decreased the expression of MEF2C and HDAC7, disrupting their interaction and promoting the overexpression of Nur77. These findings suggest that CC1007 may be a promising drug for the treatment of multiple myeloma.
BIOORGANIC CHEMISTRY
(2022)
Article
Neurosciences
Feng Xue, Jing Tian, Chunxiao Yu, Heng Du, Lan Guo
Summary: This study found that suppressed Mef2C nuclear translocation in microglia is an early and prominent phenotype in a mouse model of brain amyloidosis, which worsened with age. The oligomeric forms of Ap42 promoted Mef2C deregulation in microglia on acute organotypic brain slices, leading to enhanced microglial activation and synapse elimination, which could be substantially attenuated by blocking IFN-I signaling.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Pharmacology & Pharmacy
Yuan Huang, Longjun Xian, Zhongjian Liu, Ling Wei, Lu Qin, Yimei Xiong, Lei Hu, Siqi Zhou, Qiang Fu, Bo Li, Yang Qin
Summary: This study revealed that activation of the AMPK alpha 2/ HNF4A/BORIS/GLUT4 signaling pathway in a low-glucose microenvironment can significantly promote the invasion and metastasis of HCC cells. HNF4A and GLUT4 may have important potential applications as prognostic or drug target molecules.
BIOCHEMICAL PHARMACOLOGY
(2022)
Review
Multidisciplinary Sciences
Rishabh Chaudhary, Vipul Agarwal, Arjun Singh Kaushik, Mujeeba Rehman
Summary: MEF2 is a crucial family of transcription factors involved in the growth and differentiation of various human cells, and has been associated with neurological conditions such as ASD. Recent studies have linked MEF2C defects to neurodevelopmental disorders like ASD.
Article
Oncology
Yunwei Li, Zhe Wang, Feng Zhao, Juan Zeng, Xianghong Yang
Summary: miR-190b is expressed at lower levels in pancreatic cancer and is associated with clinicopathological characteristics. It has suppressive effects on pancreatic cancer cell proliferation, invasion, and metastasis. Furthermore, miR-190b directly targets MEF2C and TCF4, acting as a tumor suppressor and predictor of good prognosis in pancreatic cancer.
Review
Cell Biology
Glynnis A. Garry, Rhonda Bassel-Duby, Eric N. Olson
Summary: Ischemic heart disease is the leading cause of morbidity and mortality worldwide, and finding new ways to promote cardiomyocyte regeneration is crucial. Direct reprogramming of cardiac fibroblasts into induced cardiac-like myocytes (iCMs) has shown promising potential, but there are challenges in reprogramming adult human fibroblasts.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Yu Zhang, Takahiko Nishiyama, Hui Li, Jian Huang, Ayhan Atmanli, Efrain Sanchez-Ortiz, Zhaoning Wang, Alex A. Mireault, Pradeep P. A. Mammen, Rhonda Bassel-Duby, Eric N. Olson
Summary: A study introduced an efficient gene-editing method using SaCas9 to correct the commonly affected exon 51 in DMD, which showed promising therapeutic effects in correcting DMD in a mouse model through a single AAV9 delivery system.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Multidisciplinary Sciences
Catherine A. Makarewich, Amir Z. Munir, Svetlana Bezprozvannaya, Aaron M. Gibson, Soo Young Kim, Misty S. Martin-Sandoval, Thomas P. Mathews, Luke I. Szweda, Rhonda Bassel-Duby, Eric N. Olson
Summary: New evidence suggests that certain noncoding RNA molecules actually code for microproteins. By using comparative genomics, researchers identified a microprotein called Mtlbn that is abundantly expressed in the heart. Mtlbn localizes to the inner mitochondrial membrane and interacts with subunits of the electron transport chain and respiratory supercomplexes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Peiheng Gan, Zhaoning Wang, Maria Gabriela Morales, Yu Zhang, Rhonda Bassel-Duby, Ning Liu, Eric N. Olson
Summary: This study identified a genetic defect associated with noncompaction cardiomyopathy and revealed some underlying mechanisms. The loss of Rbpms gene led to premature cardiomyocyte binucleation and cell cycle arrest, in which Rbpms mediated the isoform switching of a specific protein.
DEVELOPMENTAL CELL
(2022)
Article
Medicine, Research & Experimental
Dileep R. Karri, Yu Zhang, Francesco Chemello, Yi-Li Min, Jian Huang, Jiwoong Kim, Pradeep P. A. Mammen, Lin Xu, Ning Liu, Rhonda Bassel-Duby, Eric N. Olson
Summary: This study demonstrates the long-term durability of CRISPR/Cas9 genome editing as a therapy for maintaining the integrity and function of DMD muscle, even under conditions of stress.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Geriatrics & Gerontology
Mina Shahriyari, Md Rezaul Islam, Sadman M. Sakib, Malte Rinn, Anastasia Rika, Dennis Krueger, Lalit Kaurani, Verena Gisa, Mandy Winterhoff, Harithaa Anandakumar, Orr Shomroni, Matthias Schmidt, Gabriela Salinas, Andreas Unger, Wolfgang A. Linke, Jana Zschuentzsch, Jens Schmidt, Rhonda Bassel-Duby, Eric N. Olson, Andre Fischer, Wolfram-Hubertus Zimmermann, Malte Tiburcy
Summary: This study successfully established methods for deriving skeletal muscle cells from human pluripotent stem cells and engineered a functional human skeletal muscle organoid and engineered skeletal muscle with regeneration-competent satellite-like cells. Contractile performance of the engineered muscle was further enhanced by thyroid hormone treatment.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Medicine, Research & Experimental
Yu Zhang, Hui Li, Takahiko Nishiyama, John R. McAnally, Efrain Sanchez-Ortiz, Jian Huang, Pradeep P. A. Mammen, Rhonda Bassel-Duby, Eric N. Olson
Summary: In this study, a humanized DMD mouse model was created to evaluate the efficacy of CRISPR-Cas9 gene editing for DMD correction. The model successfully restored dystrophin expression and improved pathological features and muscle strength. This model provides a valuable tool for assessing clinically relevant gene editing strategies and advancing the therapeutic translation of CRISPR-Cas9 gene editing for DMD correction.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Cell Biology
Takahiko Nishiyama, Yu Zhang, Miao Cui, Hui Li, Efrain Sanchez-Ortiz, John R. McAnally, Wei Tan, Jiwoong Kim, Kenian Chen, Lin Xu, Rhonda Bassel-Duby, Eric N. Olson
Summary: Mutations in the RBM20 gene are a common cause of familial dilated cardiomyopathy. The study uses gene editing techniques to correct these mutations and demonstrates that correction can restore normal gene splicing and nuclear localization, while eliminating abnormal ribonucleoprotein granule formation. The effectiveness of this therapy is also validated in a mouse model.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Xurde M. Caravia, Andres Ramirez-Martinez, Peiheng Gan, John R. McAnally, Rhonda Bassel-Duby, Ning Liu, Eric N. Olson
CIRCULATION RESEARCH
(2022)
Article
Medicine, Research & Experimental
Xurde M. Caravia, Andres Ramirez-Martinez, Peiheng Gan, Feng Wang, John R. McAnally, Lin Xu, Rhonda Bassel-Duby, Ning Liu, Eric N. Olson
Summary: Mutations in nuclear envelope proteins cause devastating genetic diseases that primarily affect the heart and skeletal muscle. This study reveals the essentiality of LEMD2 for genome stability and cardiac function, and uncovers its mechanistic association with human disease. Gene therapy targeting Lemd2 can rescue the disease phenotype.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Miao Cui, Ning Liu, Rhonda S. Bassel-Duby, Eric N. Olson
CIRCULATION RESEARCH
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Peiheng Gan, Rhonda S. Bassel-Duby, Ning Liu, Eric N. Olson
CIRCULATION RESEARCH
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Takahiko Nishiyama, Yu Zhang, John Mcanally, Lin Xu, Ning Liu, Rhonda S. Bassel-Duby, Eric N. Olson
Meeting Abstract
Cardiac & Cardiovascular Systems
N. M. Stege, T. R. Eijgenraam, V. Oliveira Nunes Teixeira, A. M. Feringa, E. M. Schouten, C. A. Makarewich, R. Bassel-Duby, E. N. Olson, R. A. De Boer, H. H. W. Sillje
EUROPEAN JOURNAL OF HEART FAILURE
(2022)
Article
Medicine, Research & Experimental
Andres Ramirez-Martinez, Yichi Zhang, Marie-Jose Van den Boogaard, John R. McAnally, Cristina Rodriguez-Caycedo, Andreas C. Chai, Francesco Chemello, Maarten P. G. Massink, Inge Cuppen, Martin G. Elferink, Robert J. J. van Es, Nard G. Janssen, Linda P. A. M. Walraven-van Oijen, Ning Liu, Rhonda Bassel-Duby, Richard H. van Jaarsveld, Eric N. Olson
Summary: The study identifies a variant of the MYMX gene that is associated with CFZS-like abnormalities, leading to impaired fusion activity. Patient-derived cells and mouse models confirm the pathogenic role of this variant and CRISPR/Cas9 gene editing shows potential for therapeutic intervention.
JOURNAL OF CLINICAL INVESTIGATION
(2022)