期刊
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
卷 305, 期 3, 页码 1256-1266出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajplung.00056.2013
关键词
proteomics; airway surface liquid; bronchoalveolar lavage; porcine lung
资金
- Cystic Fibrosis Foundation RDP
- National Heart, Lung, and Blood Institute [P50 HL-61234, P01 HL-091842]
- Roy J. Carver Charitable Trust
- National Cancer Institute Cancer Center Support Grant
- Sandler Family Foundation
- Gordon and Betty Moore Foundation
The airway mucosa and the alveolar surface form dynamic interfaces between the lung and the external environment. The epithelial cells lining these barriers elaborate a thin liquid layer containing secreted peptides and proteins that contribute to host defense and other functions. The goal of this study was to develop and apply methods to define the proteome of porcine lung lining liquid, in part, by leveraging the wealth of information in the Sus scrofa database of Ensembl gene, transcript, and protein model predictions. We developed an optimized workflow for detection of secreted proteins in porcine bronchoalveolar lavage (BAL) fluid and in methacholine-induced tracheal secretions [airway surface liquid (ASL)]. We detected 674 and 3,858 unique porcine-specific proteins in BAL and ASL, respectively. This proteome was composed of proteins representing a diverse range of molecular classes and biological processes, including host defense, molecular transport, cell communication, cytoskeletal, and metabolic functions. Specifically, we detected a significant number of secreted proteins with known or predicted roles in innate and adaptive immunity, microbial killing, or other aspects of host defense. In greatly expanding the known proteome of the lung lining fluid in the pig, this study provides a valuable resource for future studies using this important animal model of pulmonary physiology and disease.
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