Article
Biochemistry & Molecular Biology
Stephen C. Thornquist, Maximilian J. Pitsch, Charlotte S. Auth, Michael A. Crickmore
Summary: Research has found that a male fly's decision to persist in mating is based on a biochemical computation rather than patterns of fast electrical activity. In this computation, each neuron in a recurrent network contains slightly different internal molecular estimates, which are contrasted with input from other neurons through Protein kinase A (PKA) activity to generate a synchronized burst of calcium influx called an eruption. This biochemical activity is the key currency directing behavior and motivational state, playing a role invisible to most large-scale recording techniques.
Review
Endocrinology & Metabolism
T. John Martin
Summary: Parathyroid hormone (PTH) activates adenylyl cyclase in cells of the osteoblast lineage, indirectly regulating osteoclast formation. PTH1R, shared by PTH and PTHrP, activates adenylyl cyclase with similar potency. PTH1R is translocated from the plasma membrane to the endosome, but the influence of endosomal translocation on PTH1R activity is not yet known.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Thi Mong Diep Nguyen, Laura Filliatreau, Daniele Klett, Nong Van Hai, Nguyen Thuy Duong, Yves Combarnous
Summary: The research reveals that ADCY10 is located in the cytoplasm and responds to four different inhibitors. These inhibitors can reduce cAMP accumulation in MTLC-1 cells and various Leydig cells, emphasizing the importance of ADCY10 in response to the hormone LH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Physiology
Katrina F. Ostrom, Justin E. LaVigne, Tarsis F. Brust, Roland Seifert, Carmen W. Dessauer, Val J. Watts, Rennolds S. Ostrom
Summary: Adenylyl cyclases (ACs) catalyze the conversion of ATP to cAMP, serving as major effectors of G protein-coupled receptors (GPCRs) with varying expression patterns across tissues. Understanding the potential roles of each isoform holds promise for uncovering new therapeutic approaches.
PHYSIOLOGICAL REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Sahar Aminzai, Tingfei Hu, Renate B. Pilz, Darren E. Casteel
Summary: This study found that purified PKGI alpha can be oxidized and activated during storage, indicating that oxidation and trace metals present during storage play a role in PKGI alpha activation. Previous research suggested that certain amino acids in PKGI alpha are key for oxidation-induced activation. However, there is currently no direct evidence of oxidation-induced PKGI alpha activation in vivo.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Jun Xu, Nobuo Koizumi, Yusuke V. Morimoto, Ryo Ozuru, Toshiyuki Masuzawa, Shuichi Nakamura
Summary: Nucleotide second messengers play crucial roles in signal transduction in various organisms. A study has found that the swimming motility of the soil bacterium Leptospira kobayashii can be rapidly modulated by light stimulation. Through analysis of a loss-of-photoresponsivity mutant, a novel sensory gene was identified, and the light-dependent synthesis of cyclic adenosine monophosphate (cAMP) was elucidated. These findings reveal a new role for cAMP in controlling the flagella-dependent motility of Leptospira and highlight the global distribution of the newly discovered photoactivated cyclase among diverse microbial species.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Xuefeng Zhang, Alejandro Pizzoni, Kyoungja Hong, Nyla Naim, Chao Qi, Volodymyr Korkhov, Daniel L. Altschuler
Summary: CAP1 is a highly conserved protein involved in regulating cAMP dynamics in mammalian cells by binding and activating adenylyl cyclase, modulating cAMP levels in a Rap1-dependent manner, and affecting cAMP-dependent proliferation. The interaction between CAP1, cyclase, and Rap1 forms a regulatory unit that may be involved in establishing cAMP microdomains and localized signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Sarah Baudet, Yvrick Zagar, Fiona Roche, Claudia Gomez-Bravo, Sandrine Couvet, Johann Becret, Morgane Belle, Juliette Vougny, Sinthuya Uthayasuthan, Oriol Ros, Xavier Nicol
Summary: The study demonstrates that different axon guidance cues induce distinct intracellular signals, including cAMP, cGMP, and Ca2+, confined to separate cellular nanodomains. It challenges the traditional view that axon repellents and attractants share the same set of cellular messenger signals by showing that two repellent cues, ephrin-A5 and Slit1, activate spatially segregated signals. These findings reveal the importance of subcellular-specific second messenger crosstalk in controlling axonal morphology changes and pathfinding decisions.
NATURE COMMUNICATIONS
(2023)
Article
Dermatology
Jaewon You, Michael D. Reilly, Mahmoud Eljalby, Rohan Bareja, Maftuna Yusupova, Nikki S. Vyas, Jakyung Bang, Wanhong Ding, Garrett Desman, Lloyd S. Miller, Olivier Elemento, Richard D. Granstein, Jonathan H. Zippin
Summary: This study demonstrates the importance of soluble adenylate cyclase (sAC) in psoriasis pathogenesis and identifies it as a potential therapeutic target. The absence of Adcy10 gene significantly reduces the symptoms of psoriasiform dermatitis in mice and inhibits the differentiation and gene expression of Th17 cells. Moreover, topical application of sAC inhibitors shows promise in reducing psoriasiform dermatitis and IL17 gene expression in the skin, suggesting their potential as topical therapeutics for Th17-mediated skin diseases.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Cell Biology
Mina Hwang, Hyo-Sup Kim, Sang-Man Jin, Kyoo-Yeon Hur, Jae-Hyeon Kim, Moon-Kyu Lee
Summary: Thiazolidinediones are synthetic PPAR gamma ligands that can enhance insulin sensitivity and potentially increase insulin secretion from pancreatic beta-cells, but the functional role and mechanism of action in beta-cells have not been thoroughly investigated.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Zhan-Guo Gao, Ian M. Levitan, Asuka Inoue, Qiang Wei, Kenneth A. Jacobson
Summary: Protein kinase C (PKC) isoforms can enhance A(2B) adenosine receptor (AR)-mediated cAMP accumulation through activation by phorbol 12-myristate 13-acetate (PMA), but do not enhance beta(2)-adrenergic receptor-mediated cAMP accumulation. PKC activation can also induce cAMP accumulation by activating A(2B)AR with high or low E-max. These findings are important for understanding the functions of A(2B)AR and PKC.
Article
Biochemical Research Methods
Oziniel Ruzvidzo, Patience Chatukuta
Summary: This study provides evidence that LHL from Arabidopsis thaliana is a bona fide adenylyl cyclase (AC) that catalyzes the formation of cAMP from ATP, regulating key physiological processes. This finding is significant for understanding signal transduction in plants and further investigating the function of AC.
PLANT MOLECULAR BIOLOGY REPORTER
(2023)
Article
Biochemistry & Molecular Biology
Eri Kitayama, Maki Kimura, Takehito Ouchi, Masahiro Furusawa, Yoshiyuki Shibukawa
Summary: This study investigated the dynamics of intracellular cAMP in cultured human odontoblasts to understand the detailed expression patterns and role of the intracellular cAMP signaling pathway. The results showed the presence of G(alpha)s protein and various receptors (IP, 5-HT4, D-1, A(2A), and VIP) in odontoblasts. Activation of these receptors increased intracellular cAMP levels by activating AC, and this increase was inhibited by an AC inhibitor and receptor antagonists.
Article
Biochemistry & Molecular Biology
Jung-Chin Chang, Simei Go, Eduardo H. Gilglioni, Suzanne Duijst, Daan M. Panneman, Richard J. Rodenburg, Hang Lam Li, Hsu-Li Huang, Lonny R. Levin, Jochen Buck, Arthur J. Verhoeven, Ronald P. J. Oude Elferink
Summary: Inhibiting sAC leads to increased extracellular lactate production and decreased pyruvate production, reflecting altered cytosolic NADH/NAD(+) ratio. sAC inhibition specifically suppresses complex I of the mitochondrial respiratory chain, while activating Epac1 of cAMP signaling increases cytosolic NADH/NAD(+) ratio, regulating cellular oxidative phosphorylation and glycolysis to maintain energy balance within the cell.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Neurosciences
Ming Zhang, Hongbing Wang
Summary: The type 1 and 8 adenylyl cyclase (ADCY1 and ADCY8) are responsible for Ca2+-stimulated cyclic AMP (cAMP) production and play a role in activity-dependent synaptic modification. This study found that Adcy8(-/-) mice showed normal long-term potentiation (LTP), while Adcy1(-/-) mice showed deficits in LTP depending on the induction protocol. ADCY1 was found to be necessary for long-term depression (LTD), whereas ADCY8 was not. Both Adcy1(-/-) and Adcy8(-/-) mice showed defects in synaptic depotentiation, with Adcy8(-/-) mice's deficits depending on the induction protocol. ADCY1 was required for both initial and reversal memory formation, while ADCY8 was only necessary for reversal memory formation. These findings demonstrate that ADCY1 and ADCY8 have distinct roles in regulating synaptic and cognitive flexibility through bidirectional modulation of synaptic function.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Cardiac & Cardiovascular Systems
Hao-Liang Xu, Lizhen Mao, Shuhua Ye, Chanannait Paisansathan, Francesco Vetri, Dale A. Pelligrino
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2008)
Article
Cardiac & Cardiovascular Systems
Hao-Liang Xu, Francesco Vetri, Hae-Kyung Lee, Shuhua Ye, Chanannait Paisansathan, Lizhen Mao, Fulong Tan, Dale A. Pelligrino
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2009)
Article
Cardiac & Cardiovascular Systems
Francesco Vetri, Haoliang Xu, Lizhen Mao, Chanannait Paisansathan, Dale A. Pelligrino
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2011)
Article
Cardiac & Cardiovascular Systems
Francesco Vetri, Haoliang Xu, Chanannait Paisansathan, Dale A. Pelligrino
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2012)
Article
Neurosciences
Bin Shen, Francesco Vetri, Lizhen Mao, Hao-Liang Xu, Chanannait Paisansathan, Dale A. Pelligrino
Article
Neurosciences
Francesco Vetri, Rafael Chavez, Hao-Liang Xu, Chanannait Paisansathan, Dale A. Pelligrino
Article
Neurosciences
Hao-Liang Xu, Maggie Garcia, Fernando Testai, Francesco Vetri, Alexandra Barabanova, Dale A. Pelligrino, Chanannait Paisansathan
Review
Neurosciences
Dale A. Pelligrino, Hao-Liang Xu, Francesco Vetri
JOURNAL OF ALZHEIMERS DISEASE
(2010)
Article
Biochemistry & Molecular Biology
Jittiya Watcharotayangul, Lizhen Mao, Haoliang Xu, Francesco Vetri, Verna L. Baughman, Chanannait Paisansathan, Dale A. Pelligrino
JOURNAL OF NEUROCHEMISTRY
(2012)
Review
Cell Biology
Dale A. Pelligrino, Francesco Vetri, Hao-Liang Xu
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2011)
Article
Biochemistry & Molecular Biology
Marta Gomez-Gonzalo, Gabriele Losi, Angela Chiavegato, Micaela Zonta, Mario Cammarota, Marco Brondi, Francesco Vetri, Laura Uva, Tullio Pozzan, Marco de Curtis, Gian Michele Ratto, Giorgio Carmignoto
Article
Neurosciences
William J. Smith, David L. Cedeno, Samuel M. Thomas, Courtney A. Kelley, Francesco Vetri, Ricardo Vallejo
Summary: Numerous studies have shown the effectiveness of spinal cord stimulation in treating chronic neuropathic pain, but the exact mechanism remains uncertain. Recent research focuses on the role of microglial cells in chronic pain, highlighting differences between DTMP, HRP, and LRP in modulating microglial transcriptomes. The data suggests that DTMP may be more effective than traditional programming methods in modulating microglial activation transcriptomes and relieving pain.
Article
Neurosciences
Francesco Vetri, Danilo Menicucci, Dominga Lapi, Angelo Gemignani, Antonio Colantuoni