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Gastroenterology & Hepatology
Gangarao Davuluri, Nicole Welch, Jinendiran Sekar, Mahesha Gangadhariah, Khaled Alsabbagh Alchirazi, Maradumane L. Mohan, Avinash Kumar, Sashi Kant, Samjhana Thapaliya, McKenzie Stine, Megan R. McMullen, Rebecca L. McCullough, George R. Stark, Laura E. Nagy, Sathyamangla V. Naga Prasad, Srinivasan Dasarathy
Summary: Despite the high clinical significance of sarcopenia in alcohol-associated cirrhosis, the mechanisms of ethanol-induced impaired protein homeostasis are poorly understood. This study identified PP2A as a key mediator in ethanol-induced dysregulation of protein balance, suggesting potential therapeutic approaches targeting the PI3K gamma-PP2A axis.
Article
Chemistry, Medicinal
Gabriela Becker, Maria Fernanda Pessano Fialho, Evelyne Silva Brum, Sara Marchesan Oliveira
Summary: Chemotherapy-induced peripheral neuropathy (CIPN), associated with cisplatin use, is a severe clinical problem. The activation of kinin B-2 receptors and sensitization of the TRPA1 channel are involved in cisplatin-induced peripheral neuropathy. However, the role of downstream signaling pathways in this model remains unknown.
Article
Biochemistry & Molecular Biology
Danilo Ranieri, Deborah French, Flavia Persechino, Luisa Guttieri, Maria Rosaria Torrisi, Francesca Belleudi
Summary: FGFR2c and its downstream signaling PKC epsilon play a role in the invasive response and anchorage-independent growth of pancreatic ductal adenocarcinoma cells, as well as cell viability, cell migration/invasion, and actin cytoskeleton reorganization.
Article
Chemistry, Medicinal
R. Rama Suresh, Zhan-Guo Gao, Veronica Salmaso, Eric Chen, Ryan G. Campbell, Russell B. Poe, Theodore E. Liston, Kenneth A. Jacobson
Summary: The structure-activity relationship of an A3AR antagonist has been explored and a high-affinity radioligand has been developed, which shows promise as a useful tool for receptor characterization and drug discovery.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Linglian Kong, Chuanpi Xiao, Hai Lin, Johan Buyse, Xianlei Li, Zhigang Song
Summary: The study showed that CB1 is mainly expressed in the hypothalamus of broilers, and 72-hour DEX exposure can regulate the CB1 system and AMPK signaling pathway in the broiler hypothalamus. Treatment with DEX for 24 hours and 72 hours increased the mRNA levels of hypothalamic AMPK alpha 2 and GR.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Cell Biology
Shaoqing Zhang, Xiuli Gong, Yiye Zhou, Qingwen Ma, Qin Cai, Guanheng Yang, Xinbing Guo, Yanwen Chen, Miao Xu, Yiwen Zhu, Yitao Zeng, Fanyi Zeng
Summary: This study investigates the role of maternal transcripts in early embryonic development by comparing transcriptomes from oocytes of high- or low-competence mouse strains. The results suggest that the transcription level of maternal messages, such as Prkce, in mature oocytes is associated with different 2-cell rates in select inbred mouse strains. Prkce transcript levels could serve as a potential biomarker to characterize high-quality mature oocytes.
CELL PROLIFERATION
(2022)
Article
Biochemistry & Molecular Biology
Rachel Aruldas, Laura Buczek Orenstein, Sade Spencer
Summary: Repeated cocaine exposure induces changes in AMPK and pAMPK in different brain regions and cellular compartments, with some differences between acute withdrawal and ongoing cocaine treatment. Pretreatment with metformin, an indirect AMPK activator, enhances acute cocaine-induced locomotor activity but blocks the development of sensitization. These findings provide insights into the dynamics of AMPK activation in subcellular compartments and support the potential use of metformin for the treatment of cocaine use disorder.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Mingming Wang, Jiaoyan Yu, Qi Yang, Chao Guo, Wei Zhang, Weiwei Li, Yan Weng, Yi Ding, Jingwen Wang
Summary: This study demonstrated that beta-BA exerts neuroprotective effects against cerebral I/R injury by activating the PRKCE/NFE2L2/HMOX1 pathway, improving neurological deficits in animals and protecting neurons in in vitro experiments.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Carolin Torregroza, Chiara O. Glashoerster, Katharina Feige, Martin Stroethoff, Annika Raupach, Andre Heinen, Markus W. Hollmann, Ragnar Huhn
Summary: The study found that Mannitol achieves cardioprotective effects through activation of the adenosine A1 receptor, rather than the protein kinases Akt and PKG. Akt and PKG are not involved in the preconditioning cardioprotection induced by Mannitol.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Sukanya Tripathy, Bader O. Almutairi, Sanjay Singh, Atul Rawat, Durgesh Dubey, Monisha Banerjee, Dinesh R. Modi, Anand Prakash
Summary: Thrombin-activated receptor-1 (PAR1) plays a crucial role in tumor angiogenesis, cell proliferation, and invasion. The present study investigated the expression of PAR1 in human glioma cell lines D54 and U87, as well as its downstream signaling pathway. The results showed that PAR1 was highly expressed in D54 cells compared to U87 cells. Activation of PAR1 led to increased ERK1/2 activity and enhanced migration and invasion. The antagonist SCH79797 significantly inhibited the migration and invasion of the glioma cell lines. Moreover, PAR1 activation affected intracellular Ca+2 concentration ([Ca+2]i). Thrombin and TFLLR induced an increase in [Ca+2]i, which was suppressed by the PAR1 inhibitor SCH79797. The study suggests that PAR1 signaling contributes to glioma progression and may serve as a potential therapeutic target for glioma prevention.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Cell Biology
Filomena Lauro, Luigino Antonio Giancotti, Grant Kolar, Caron Mitsue Harada, Taylor A. Harmon, Timothy J. Garrett, Daniela Salvemini
Summary: The involvement of S1PR1 in neuropathic pain suggests the need for further exploration of signaling pathways downstream of S1PR1 for the development of antagonists. Experimental results indicate that IL-1 beta and ADK play significant roles in the actions of S1PR1.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jia Song, Wei Zhao, Xin Zhang, Wenyu Tian, Xuyang Zhao, Liang Ma, Yongtong Cao, Yuxin Yin, Xuehui Zhang, Xuliang Deng, Dan Lu
Summary: A study has identified frameshift germline mutations of RIG-I in colon cancer patients, which may be associated with increased susceptibility to colon cancer. The study provides insights into the action of mutant RIG-I and proposes a potential strategy for the treatment of colon cancer.
NATURE COMMUNICATIONS
(2022)
Article
Reproductive Biology
Khushbukhat Khan, Sameen Zafar, Yasmin Badshah, Naeem Mahmood Ashraf, Mehak Rafiq, Lubna Danish, Maria Shabbir, Janeen H. Trembley, Tayyaba Afsar, Ali Almajwal, Suhail Razak
Summary: This study used bioinformatics approaches to predict the structure and localization of TPD52 and analyzed its molecular interactions. Real-time PCR-based expression analysis was performed to evaluate its co-expression with other oncogenic pathway proteins in ovarian cancer.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Endocrinology & Metabolism
Maria Bove, Fermi Monto, Paloma Guillem-Llobat, M. Dolores Ivorra, M. Antonia Noguera, Andrea Zambrano, M. Salome Sirerol-Piquer, Ana Cristina Requena, Mauricio Garcia-Alonso, Teresa Tejerina, Jose T. Real, Isabel Farinas, Pilar D'Ocon
Summary: Investigation on human and murine adipose tissue revealed the presence of NT3/TrkC pathway, suggesting its role in lipolysis and adipocyte differentiation.NT3 activation of TrkC regulates adipocyte differentiation and browning, impacting adipocyte size and expression of thermogenesis marker UCP-1.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Chemistry, Medicinal
Eleonora Elhalem, Ana Bellomo, Mariana Cooke, Antonella Scravaglieri, Larry Pearce, Megan L. Peach, Lucia Gandolfi Donadio, Marcelo G. Kazanietz, Maria J. Comin
Summary: DAG-lactones are useful templates for designing potent and selective ligands for PKC isozymes, with the ester moiety at the sn-1 position playing a critical role in C1 domain interactions. By introducing a stable isoxazole ring as an ester surrogate in DAG-lactones, it is possible to achieve selectivity for PKC isozymes.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)