Article
Gastroenterology & Hepatology
Gangarao Davuluri, Nicole Welch, Jinendiran Sekar, Mahesha Gangadhariah, Khaled Alsabbagh Alchirazi, Maradumane L. Mohan, Avinash Kumar, Sashi Kant, Samjhana Thapaliya, McKenzie Stine, Megan R. McMullen, Rebecca L. McCullough, George R. Stark, Laura E. Nagy, Sathyamangla V. Naga Prasad, Srinivasan Dasarathy
Summary: Despite the high clinical significance of sarcopenia in alcohol-associated cirrhosis, the mechanisms of ethanol-induced impaired protein homeostasis are poorly understood. This study identified PP2A as a key mediator in ethanol-induced dysregulation of protein balance, suggesting potential therapeutic approaches targeting the PI3K gamma-PP2A axis.
Article
Biochemistry & Molecular Biology
Rachel Aruldas, Laura Buczek Orenstein, Sade Spencer
Summary: Repeated cocaine exposure induces changes in AMPK and pAMPK in different brain regions and cellular compartments, with some differences between acute withdrawal and ongoing cocaine treatment. Pretreatment with metformin, an indirect AMPK activator, enhances acute cocaine-induced locomotor activity but blocks the development of sensitization. These findings provide insights into the dynamics of AMPK activation in subcellular compartments and support the potential use of metformin for the treatment of cocaine use disorder.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Anthony Veltri, Christopher M. R. Lang, Gaia Cangiotti, Chim Kei Chan, Wen-Hui Lien
Summary: The study reveals the important roles of the Wnt receptor ROR2 in the activation, self-renewal, and maintenance of hair follicle stem cells (HFSCs), through both transducing Wnt signaling and regulating DNA damage response.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Chenchen Li, Emma E. Furth, Anil K. Rustgi, Peter S. Klein
Summary: The Wnt signaling pathway is crucial in metazoan development and stem cell maintenance, and is also involved in various malignancies. Apart from the canonical activation, the pathway can affect cell function through multiple alternative effectors, potentially contributing to cancer development.
Article
Biochemistry & Molecular Biology
Chunyang Du, Yan Zhu, Yan Yang, Lin Mu, Xue Yan, Ming Wu, Chenming Zhou, Haijiang Wu, Wei Zhang, Yanhui Wu, Guoyu Zhang, Yue Hu, Yunzhuo Ren, Yonghong Shi
Summary: CTRP3 plays a role in lipid metabolism and necroinflammation in renal tubular cells by inhibiting SREBP-1 expression, increasing PPAR-α and ABCA1 expression, and inhibiting NLRP3-mediated inflammation and MLKL-dependent necroinflammation. It also activates the AMPK pathway to provide protection.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Panfeng Wang, Yuntong Zhang, Yan Xia, Dayuan Xu, Hongrui Wang, Dong Liu, Shuogui Xu, Yongming Sun
Summary: The treatment with miR-139-5p enhanced functional recovery and reduced pain hypersensitivity in mice with SCI, possibly through targeting Mst1.
NEUROCHEMICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jaeyoon Kim, Jae Young Shin, Yun-Ho Choi, So Young Lee, Mu Hyun Jin, Chang Deok Kim, Nae-Gyu Kang, Sanghwa Lee
Summary: Adenosine and cordycepin can promote wound healing process through activation of adenosine receptors and stimulation of the Wnt/beta-catenin signaling pathway, enhancing mitochondrial energy metabolism, cell proliferation, and gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Vikash Singh, Vonn Walter, Irina Elcheva, Yuka Imamura Kawasawa, Vladimir S. Spiegelman
Summary: In this study, the role of IGF2BP1 in the post-transcriptional regulation of Wnt/beta-catenin-induced genes in colorectal cancer (CRC) cells was investigated using iCLIP and RNA sequencing techniques. The study revealed that CRC cells exhibit a stronger dependency on IGF2BP1 expression for Wnt/beta-catenin-regulated genes compared to normal cells. iCLIP analysis showed significant changes in IGF2BP1-binding sites and enrichment of 6-mer motifs associated with IGF2BP1 binding in response to Wnt/beta-catenin signaling. Additionally, a signature of IGF2BP1-regulated genes significantly associated with colon cancer-free survival in humans was identified.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Rui Xie, Kunlong Yang, Elisabeth Tumukunde, Zhiqiang Guo, Bei Zhang, Yinghang Liu, Zhenhong Zhuang, Jun Yuan, Shihua Wang
Summary: This study identified six RGS proteins in Aspergillus flavus and revealed their important functions in regulating conidia, sclerotia, and aflatoxin formation. The results provide valuable information for controlling the contamination of A. flavus and mycotoxins produced by this fungus in agricultural crops.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Qun Wei, Hui Ren, Jun Zhang, Wen Yao, Baoxiang Zhao, Junying Miao
Summary: HCP1 inhibited oxLDL-induced VEC injury and promoted the stabilization of atherosclerotic plaque in apoE(-/-) mice, suggesting that it may be a potential therapeutic target for the treatment of atherosclerosis.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Maria Cristina Procopio, Rita Lauro, Chiara Nasso, Scipione Carerj, Francesco Squadrito, Alessandra Bitto, Gianluca Di Bella, Antonio Micari, Natasha Irrera, Francesco Costa
Summary: Myocardial infarction is often caused by atherosclerotic plaque erosion or rupture leading to thrombotic occlusion of a coronary vessel. The inadequate oxygen and nutrient supply in the infarcted area can result in cardiomyocyte necrosis and left ventricular function impairment. The adenosine system, purinergic system, and Wnt/beta-catenin pathway may play crucial roles in the pathogenesis of cardiac damage following MI, with potential therapeutic implications in preventing adverse LV remodeling and heart failure.
Article
Biochemistry & Molecular Biology
Li-Li Wang, Yao Du, Shu-Min Li, Fei Cheng, Na-Na Zhang, Rui Chen, Xing Cui, Sheng-Gang Yang, Ling-Ling Fan, Jian-Ta Wang, Bing Guo, Hao-Shu Wu, Ji-Quan Zhang, Lei Tang
Summary: Two series of tetrahydrocarbazole derivatives based on a promising candidate ZG02 were designed and synthesized, and compound 12b showed potent hypoglycemic activity by potentially activating the AMPK pathway. Metabolic stability assays and OGTT results confirmed 12b as an effective hypoglycemic agent.
BIOORGANIC CHEMISTRY
(2021)
Article
Biochemical Research Methods
Kevin W. Cormier, Brett Larsen, Anne-Claude Gingras, James R. Woodgett
Summary: The functional differences between the two isoforms of glycogen synthase kinase-3 (GSK-3), a protein-serine/threonine kinase, are still not fully understood. While the isoforms are structurally similar and redundant, there is evidence for specific roles. This study aimed to identify novel GSK-3 interaction partners and compare the interactomes of the two isoforms using affinity purification and proximity-dependent biotinylation mass spectrometry methods. The results showed that the interactomes of the isoforms were highly similar in HEK293 cells, but there were differences in HeLa cells, suggesting isoform-specific associations with certain proteins.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Kevin W. Cormier, Brett Larsen, Anne-Claude Gingras, James R. Woodgett
Summary: This study investigates the functional differentiation of two isoforms of GSK-3 and identifies novel interaction partners using affinity purification and proximity-dependent biotinylation mass spectrometry methods. The interactomes of the two isoforms are highly similar in HEK293 cells, but there are isoform-specific interactions in HeLa cells. Understanding the differences in interactions between the isoforms may provide insights into their distinct functions and regulation, and open up possibilities for isoform-specific strategies.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Immunology
Runxiao Lv, Lili Du, Lunhao Bai
Summary: This study finds that RNF125 plays an important role in osteoarthritis (OA). Moderate physical exercise can protect cartilage, while the overexpression of RNF125 can inhibit the degradation of extracellular matrix in chondrocytes. RNF125 may be a novel biomarker for OA therapy.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hongwei Liu, Shenghao Liu, Huijuan Wang, Kaoshan Chen, Pengying Zhang
Summary: The study found that the cloned flavonoid 3 '-hydroxylase gene (PnF3'H) from Antarctic moss plays an important role in adapting to polar environments by regulating oxidative tolerance and ABA sensitivity, enhancing plants' ability to adapt to extreme conditions.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Plant Sciences
Huijuan Wang, Hongwei Liu, Qian Yu, Fenghua Fan, Shenghao Liu, Guihua Feng, Pengying Zhang
Summary: Organisms in the Antarctic continent are exposed to high levels of UV radiation due to damaged stratospheric ozone. The isolated CPD photolyase gene PnPHR1 from Antarctic moss Pohlia nutans encodes a protein that can repair UV-damaged DNA and enhance plant tolerance to UV radiation and salinity stress. This study shows that PnPHR1 may play a crucial role in the adaptation of P. nutans to polar environments.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Engineering, Environmental
Bailin Cong, Shuang Li, Shenghao Liu, Wenying Mi, Shengfa Liu, Zhaohui Zhang, Zhiyong Xie
Summary: The distribution of persistent organic pollutants (POPs) in the Eastern Indian Ocean (EIO) was investigated. The study provides insights into the characteristics, sources, and driving factors of POPs in the region.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yiqing Ma, Fangrui Lou, Xiaofei Yin, Bailin Cong, Shenghao Liu, Linlin Zhao, Li Zheng
Summary: This study utilized whole-genome sequencing data to investigate the genomic characteristics and phylogenetic relationship of Pacific cod (Gadus macrocephalus). The study revealed that the estimated genome size of Pacific cod was approximately 658.22 Mb, and it contained a high proportion of repetitive sequences. Analysis of the mitochondrial genome provided insights into the evolutionary relationships between Pacific cod and its relatives. Additionally, demographic history analysis indicated changes in the population of Pacific cod during the Pleistocene.
BIOSCIENCE REPORTS
(2022)
Article
Plant Sciences
Huijuan Wang, Shenghao Liu, Fenghua Fan, Qian Yu, Pengying Zhang
Summary: Flavonoids, a group of polyphenolic secondary metabolites present in all land plants, play important roles in various biological processes and defense against abiotic stresses. In this study, researchers cloned a novel 2-ODD gene from Antarctic moss and found that its expression in model plants increased the accumulation of anthocyanins and flavonols, as well as enhanced tolerance to salinity, drought, UV-B radiation, and oxidative stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Shenghao Liu, Shuo Fang, Bailin Cong, Tingting Li, Dan Yi, Zhaohui Zhang, Linlin Zhao, Pengying Zhang
Summary: In this study, we assembled the high-quality genome sequence of the Antarctic moss Pohlia nutans and found that it has a larger genome size compared to other bryophytes. The genome of P. nutans contains a high number of gene duplications and expansions of gene families, which may contribute to its adaptation to extreme environments. Genes related to phenylpropanoid biosynthesis, unsaturated fatty acid biosynthesis, and plant hormone signal transduction are expanded in the genome of P. nutans, potentially supporting its lifestyle in Antarctica. Flavonoid biosynthesis may function in detoxification of reactive oxygen species under UV-B radiation, as revealed by integrated multi-omic analyses.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Shuo Fang, Tingting Li, Pengying Zhang, Chenlin Liu, Bailin Cong, Shenghao Liu
Summary: This study revealed the molecular mechanisms of an Antarctic moss adapting to drought stress. Drought stress caused the gametophytes of the moss to turn yellow and curl, and increased the contents of malondialdehyde and proline, as well as the activities of antioxidant enzymes. Transcriptome and metabolome analyses showed differential gene expression and changes in metabolite levels in response to drought treatment. The genes and metabolites involved in antioxidant, flavonoid, and lipid metabolism were found to play important roles in the adaptation to drought stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Genetics & Heredity
Li Wang, Shouqiang Wang, Ye Lin, Shenghao Liu, Panjiao Liang, Linlin Zhao
Summary: The complete mitochondrial genome of Upeneus japonicus was successfully assembled using high-throughput sequencing data in this study. It consists of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a control region. Phylogenetic analysis revealed that U. japonicus is closely related to its sister species U. tragula. This study lays the foundation for further research on population genetics and evolutionary analysis in U. japonicus.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Environmental Sciences
Linlin Zhao, Shenghao Liu, Shouqiang Wang, Tianyan Yang, Qi Liu, Tianxiang Gao
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Wenqi Liu, Bailin Cong, Jing Lin, Linlin Zhao, Shenghao Liu
Summary: In this study, the whole genomes of two strains were sequenced to clarify the basic characteristics of their genomes, laying the foundation for further studying nitrogen-metabolizing bacteria. Halomonas profundus 13 can utilize more carbon sources than Cobetia amphilecti N-80, as indicated by API as well as COG and KEGG prediction results. Finally, through the analysis of the nitrification and denitrification abilities as well as the inorganic carbon fixation ability of the two strains, the related genes were identified, and the possible metabolic pathways were predicted. Together, these results provide molecular markers and theoretical support for the mechanisms of inorganic carbon fixation by deep-sea microorganisms.
Article
Plant Sciences
Shenghao Liu, Tingting Li, Shuo Fang, Pengying Zhang, Dan Yi, Bailin Cong, Zhaohui Zhang, Linlin Zhao
Summary: By using an integrated multi-omics approach, this study investigated the global metabolites and regulatory pathways in an Antarctic moss under cold stress. The results revealed changes in proline content, antioxidant enzyme activities, chlorophyll, and flavonoids. Metabolome and transcriptome analyses suggested that jasmonate signaling, auxin signaling, fatty acids biosynthesis, and flavonoid biosynthesis pathways contribute to the adaptation of the moss to cold stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Shuang Li, Hui Zhang, Bailin Cong, Peiqing He, Wenqi Liu, Shenghao Liu
Summary: A method for preparing biogenic silver and silver chloride nanoparticles (Ag@AgCl NPs) was developed using bacteria from the Arctic Ocean. The nanoparticles showed good dispersion and antimicrobial activity, and could be used for dye degradation and other applications. The synthesis mechanism of the nanoparticles might be related to specific functional groups in the supernatant of the bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Fangyuan Qu, Shouqiang Wang, Wei Wang, Shenghao Liu, Shuyun Li, Haidan Liu, Zhaohui Zhang
Summary: We conducted a survey comparing the macrobenthic community structure and sediment properties between an invaded area and a bare mud flat. The results showed no significant difference in biomass, abundance, species number, or biodiversity between the invaded area and the mud flat. However, higher heavy metal concentrations and altered community structure were observed in the invaded area. The growth stage of S. alterniflora might be an important factor when assessing its impact on the macrobenthic community structure.
WETLANDS ECOLOGY AND MANAGEMENT
(2023)
Article
Genetics & Heredity
Panjiao Liang, Shouqiang Wang, Ye Lin, Li Wang, Linlin Zhao, Shenghao Liu
Summary: This study sequenced and analyzed the complete mitochondrial genome of Cepola schlegelii, revealing its close relationship with Acanthocepola krusensternii. The complete mitochondrial sequence of C. schlegelii will enrich the mitochondrial genome database and provide useful resources for population genetics and evolution analysis.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Biochemistry & Molecular Biology
Shuo Fang, Bailin Cong, Linlin Zhao, Chenlin Liu, Zhaohui Zhang, Shenghao Liu
Summary: Antarctic organisms are facing multiple environmental pressures, such as the strong UV radiation resulting from the ozone layer depletion. The molecular mechanisms and regulatory networks of Antarctic plants, particularly mosses, in response to UV-B radiation remain largely unknown.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)