Article
Biochemistry & Molecular Biology
Yinna Wang, Guoning Liao, Ruping Wang, Dale D. Tang
Summary: Actin cytoskeletal reorganization is crucial for regulating smooth muscle contraction, with Abi1 playing a key role in this process through acetylation at K416. The interaction between Abi1 and N-WASP enhances actin polymerization, ultimately promoting smooth muscle contraction. Additionally, the localization of p300 in the cytoplasm of smooth muscle cells suggests a novel mechanism for the acetylation-dependent regulation of the actin cytoskeleton.
Article
Multidisciplinary Sciences
Yoshiaki Suzukia, Takumi Ozawa, Tomo Kurata, Nanami Nakajima, Gerald W. Zamponi, Wayne R. Giles, Yuji Imaizumi, Hisao Yamamura
Summary: Elevation of intracellular Ca2+ concentration activates Ca2+/calmodulin-dependent kinases (CaMK) and promotes gene transcription. The complex of Ca(v)1.2/CaMKK2/CaMK1a located in caveolae plays a crucial role in vascular myocytes, converting changes in Ca2+ influx into gene transcription, ultimately leading to macrophage accumulation and media remodeling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Allergy
Thomas J. A. Maguire, Stephanie Yung, Elena Ortiz-Zapater, O. Stephanie Kayode, Stephen Till, Chris Corrigan, Leonard Q. C. Siew, Gregory A. Knock, Grzegorz Woszczek
Summary: S1P plays an important role in regulating airway smooth muscle reactivity and hyperplasia, potentially serving as a novel therapeutic target for asthma and other lung diseases.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alvaro Yogi, Marina Rukhlova, Claudie Charlebois, Ganghong Tian, Danica B. Stanimirovic, Maria J. Moreno
Summary: The study found that VSMCs differentiated from adipose-derived stem cells (ASCs) show potential for populating PET-based vascular scaffolds. By shortening the differentiation period and studying the role of downstream effectors of TGF-beta, the process was stabilized. The non-woven PET structure did not affect ASC differentiation, providing a novel alternative to address issues with long-term patency of vascular grafts.
Article
Biology
Xueyong Wang, Murad Nawaz, Chris DuPont, Jessica H. Myers, Steve Ra Burke, Roger A. Bannister, Brent D. Foy, Andrew A. Voss, Mark M. Rich
Summary: Excitation-contraction coupling is the process of converting electrical excitation of muscle into force generation. Depolarization of skeletal muscle can lead to failure of ECC, specifically the failure to generate calcium transients. Different AP properties, such as AP peak and AP integral, play important roles in different steps of ECC, including AP conduction and calcium release.
Article
Biochemistry & Molecular Biology
Mohammad Alqudah, Rima Abdul Razzaq, Mahmoud A. Alfaqih, Othman Al-Shboul, Ahmed Al-Dwairi, Safa Taha
Summary: This study aimed to investigate the signaling pathways involved in oxytocin-induced contractions in gastric smooth muscle, and found that oxytocin directly stimulates its cell surface receptor to activate PLC beta 1, which in turn liberates IP3, leading to an elevation in intracellular calcium and smooth muscle contraction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Physiology
Walter C. Thompson, Paul H. Goldspink
Summary: This review focuses on the crucial role of 14-3-3 proteins in regulating excitation-contraction coupling in muscle, highlighting the need for further investigation into their mechanistic actions in force generation.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yong-sheng Wang, Guo-qing Teng, Han Zhou
Summary: This study highlights that inflammation caused by selenium deficiency inhibits the contraction of smooth muscle in the small intestine, and elucidates this relationship at the molecular and pathological levels.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Physiology
Goutham Pattabiraman, Ashlee J. Bell-Cohn, Stephen F. Murphy, Daniel J. Mazur, Anthony J. Schaeffer, Praveen Thumbikat
Summary: The study found that mast cells play a critical role in urinary dysfunction in BPH/LUTS patients and mouse models. Inhibiting mast cells can alleviate both pathology and symptoms, suggesting mast cell inhibition as a potential therapeutic target for BPH/LUTS.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Hematology
Katherine M. Owsiany, Rebecca A. Deaton, Karen G. Soohoo, Anh Tram Nguyen, Gary K. Owens
Summary: This study investigates the contribution of MCP1 produced by classical smooth muscle cells (SMCs) and Lgals3-transitioned SMCs in atherosclerosis. The results show that MCP1 produced by classical SMCs has an atheroprotective effect, while MCP1 produced by Lgals3-transitioned SMCs exacerbates plaque pathogenesis. These findings highlight the need for caution when considering therapeutic interventions involving MCP1.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Physiology
Li Zhang, Xi-Xi Zeng, Yu-Mei Li, Shao-Kun Chen, Li-Yu Tang, Nan Wang, Xi Yang, Mo-Jun Lin
Summary: The study suggests that keratin 1 regulates PASMC expansion and has a preventive effect on PAH.
Review
Physiology
Pura Bolanos, Juan C. Calderon
Summary: The excitation-contraction coupling in skeletal muscle is a crucial link between membrane excitation and mechanical contraction mediated by Ca2+, involving multiple mechanisms of Ca2+ release and recycling.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lara Hairapetian, Enrico Cortes, Junyi Zhao, Yao Wang, Ricky Huang, Robert Damoiseaux, Ivan Pushkarsky
Summary: Cellular contractile force is a fundamental trait shared by all cells, and it is crucial for development and function at both cellular and tissue levels. This force also regulates mechanical systems in the body. Various biological processes, including cell motility, adhesion, division, and organ contraction, rely on force. Exaggerated or disrupted cellular contractility can lead to disease processes. This article presents a comprehensive protocol for a microplate-based contractility assay technology called FLECS, which simplifies the analysis of single-cell contractility. This technology allows researchers to study this difficult-to-quantify cell phenotype, effectively lowering the entry barrier into the field of force biology and phenotypic screening of contractile cell force.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Review
Developmental Biology
Michael Donadon, Massimo M. Santoro
Summary: The article primarily describes the developmental origin of smooth muscle cells in different organs, as well as their specification and differentiation in the cardiovascular, respiratory, and intestinal systems. It discusses the important roles of smooth muscle in organ development and how they instruct the development of other organs through signaling and mechanical feedback mechanisms. By understanding smooth muscle cell development, the aim is to advance therapeutic approaches related to tissue regeneration and other smooth muscle-related diseases.
Article
Biochemistry & Molecular Biology
Dmitry Shishmarev, Emily Rowland, Shouvik Aditya, Srinivasan Sundararaj, Aaron J. Oakley, Angela F. Dulhunty, Marco G. Casarotto
Summary: Excitation-contraction coupling is the process by which an electrical signal is converted into muscle contraction. In this study, the interaction between two calcium channels, dihydropyridine receptor (DHPR) and ryanodine receptor 1 (RyR1), during the excitation-contraction coupling process was investigated. The results showed that the protein STAC3 interacts with a subunit of the DHPR known as the II-III loop, and this interaction differs from the interaction with the neuronal isoform STAC2. These findings provide insights into the molecular mechanism of excitation-contraction coupling in skeletal muscle.
Article
Cell Biology
Yongtong Zhao, Sandor S. Shapiro, Masumi Eto
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2016)
Article
Cell Biology
Marion J. Siegman, Masumi Eto, Thomas M. Butler
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2016)
Review
Physiology
Akira Takai, Masumi Eto, Katsuya Hirano, Kosuke Takeya, Toshiyuki Wakimoto, Masaru Watanabe
JOURNAL OF PHYSIOLOGICAL SCIENCES
(2018)
Correction
Physiology
Akira Takai, Masumi Eto, Katsuya Hirano, Kosuke Takeya, Toshiyuki Wakimoto, Masaru Watanabe
JOURNAL OF PHYSIOLOGICAL SCIENCES
(2018)
Article
Biology
Joshua J. Filter, Byron C. Williams, Masumi Eto, David Shalloway, Michael L. Goldberg
Article
Biochemistry & Molecular Biology
Masumi Eto, Jason A. Kirkbride, Rishika Chugh, Nana Kofi Karikari, Jee In Kim
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2013)
Article
Biochemistry & Molecular Biology
Mukta Khasnis, Akiko Nakatomi, Kristyn Gumpper, Masumi Eto
Article
Biochemistry & Molecular Biology
Mykhaylo Artamonov, Swapnil K. Sonkusare, Miranda E. Good, Ko Momotani, Masumi Eto, Brant E. Isakson, Thu H. Le, Eric L. Cope, Zygmunt S. Derewenda, Urszula Derewenda, Avril Somlyo
Article
Biochemical Research Methods
Masumi Eto, Shuichi Katsuki, Yoshinori Tanaka, Kosuke Takeya
Article
Cell Biology
Toshio Kitazawa, Toshiyasu Matsui, Shuichi Katsuki, Akira Goto, Kai Akagi, Naoya Hatano, Hiroshi Tokumitsu, Kosuke Takeya, Masumi Eto
Summary: In this study, it was found that Ca2+-induced Ca2+ desensitization of LC20 phosphorylation in phasic smooth muscles is caused by MLCK inactivation. Inhibition of CaMKK beta attenuated both the phasic response of contraction and MLCK phosphorylation, while inhibition of protein phosphatase 2A amplified the phasic response.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yoshinori Tanaka, Shun-ya Kusumoto, Yuki Honma, Kosuke Takeya, Masumi Eto
Summary: In this study, we found that PGRN overexpression suppresses autophagic flux and worsens the pathology of neurodegenerative diseases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Ana M. Pasapera, Sarah M. Heissler, Masumi Eto, Yukako Nishimura, Robert S. Fischer, Hawa R. Thiam, Clare M. Waterman
Summary: MARK2 is identified as a master regulator of both actomyosin and microtubule cytoskeletal systems and focal adhesions, mediating directional cancer cell migration.
Article
Biochemistry & Molecular Biology
Yoshinori Tanaka, Hirotsugu Hino, Kosuke Takeya, Masumi Eto
Summary: Macroautophagy, a cellular degradation system, plays a crucial role in disease development. This study investigates the formation of autolysosomes and their regulation by autophagosome-lysosome fusion. The researchers suggest that vacuole-like autolysosomes induced by specific drugs could be used as a tool to evaluate autophagosome-lysosome fusion and study the effects of PGRN in autophagy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Eui Kyung Youn, Hyun Min Cho, Jin Ki Jung, Ga-Eun Yoon, Masumi Eto, Jee In Kim
Summary: High-fat diet-induced obesity is a cause of resistant hypertension. Histone deacetylases (HDACs) may be linked to renal angiotensinogen (Agt) upregulation in HFD-induced hypertension, but the underlying mechanisms are unclear. In this study, HDAC1/2 inhibition and siRNA experiments revealed the roles of HDAC1 and HDAC2 in HFD-induced hypertension and identified the signaling axis between HDAC1 and Agt transcription. The abnormal HDAC1/2 in the kidney might be responsible for the upregulation of Agt gene expression and hypertension, suggesting a potential therapeutic target for obesity-associated resistant hypertension.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Yoshinori Tanaka, Shun-ichi Ito, Yuki Honma, Masato Hasegawa, Fuyuki Kametani, Genjiro Suzuki, Lina Kozuma, Kosuke Takeya, Masumi Eto
Summary: The secretion of TDP-43 is stimulated by the inhibition of the autophagy-lysosomal pathway driven by the protein progranulin (PGRN). This dysregulation promotes the cell-to-cell spread of TDP-43 and may contribute to the progression of amyotrophic lateral sclerosis and frontotemporal lobar degeneration.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)