Article
Biochemistry & Molecular Biology
Euitaek Jung, Sukjin Ou, Sung Shin Ahn, Hyunjin Yeo, Young Han Lee, Soon Young Shin
Summary: In the tumor microenvironment, tumor necrosis factor alpha (TNF-alpha) promotes the endothelial differentiation of mesenchymal stem cells (MSCs) through the activation of the JNK-EGR1 signaling axis. This finding is important for identifying potential therapeutic targets for solid tumor vasculatures.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Multidisciplinary Sciences
Luezhen Yuan, Bibhas Roy, Prasuna Ratna, Caroline Uhler, G. Shivashankar
Summary: Long-term sustained mechano-chemical signals in tissue microenvironment regulate cell-state transitions. In this study, the authors identified Lef1 as a critical somatic transcription factor for mechanically induced cell-state transitions. They showed that Lef1 knockdown resulted in the down-regulation of fibroblast dedifferentiation and identified potential signaling pathways upstream of Lef1 activation. This research describes an important mechanotransduction pathway for fibroblast dedifferentiation.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Glenn J. Markov, Thach Mai, Surag Nair, Anna Shcherbina, Yu Xin Wang, David M. Burns, Anshul Kundaje, Helen M. Blau
Summary: The AP-1 transcription factor c-Jun plays a critical role in human somatic cell reprogramming by enhancing enhancer accessibility in fibroblasts and repressing OCT4 expression. Through epigenome remodeling, successful reprogramming of human fibroblasts to induced pluripotent stem cells was achieved.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Danika S. Bakke, Jilei Zhang, Yongguo Zhang, Destiny Ogbu, Yinglin Xia, Jun Sun
Summary: Cross talk between immune cells and the intestinal crypt is critical in maintaining intestinal homeostasis. Recent studies have found that vitamin D receptor (VDR) signaling plays a direct role in intestinal and microbial homeostasis, but the specific role of immune VDR signaling is still not well understood. This study used a myeloid-specific VDR knockout mouse model and a macrophage/enteroids coculture system to investigate the tissue-specific VDR signaling in intestinal homeostasis. The findings showed that myeloid VDR regulates crypt differentiation and the microbiota in a VDR-dependent mechanism, and dysregulation of myeloid VDR increases the risk of colitis-associated diseases.
Article
Oncology
Xingying Zhang, Chenze Zhang, Miaomiao Qiao, Chen Cheng, Na Tang, Shan Lu, Wen Sun, Beilei Xu, Yuanwei Cao, Xiaofei Wei, Yao Wang, Weidong Han, Haoyi Wang
Summary: Depletion of basic leucine zipper ATF-like transcription factor (BATF) improves the antitumor performance of CAR-T cells against solid tumors, and BATF regulates genes related to exhaustion and development of effector and memory T cells.
Article
Medicine, Research & Experimental
Shengzhi Liu, Xun Sun, Kexin Li, Rongrong Zha, Yan Feng, Tomohiko Sano, Chuanpeng Dong, Yunlong Liu, Uma K. Aryal, Akihiro Sudo, Bai-Yan Li, Hiroki Yokota
Summary: The study found that tumor cells can also produce anti-tumor secretomes, which have inhibitory effects on various types of cancer and have the potential to be a treatment option for primary and metastatic cancers. In a mouse model, it was discovered that the tumor-suppressive secretome can reduce tumor growth and prevent bone destruction, with the protein Eno1 interacting with CD44 playing a role in its anti-tumor effects.
Article
Pharmacology & Pharmacy
Tobias M. Backes, Daniel S. Langfermann, Andrea Lesch, Oliver G. Rossler, Matthias W. Laschke, Charles Vinson, Gerald Thiel
Summary: The study revealed that activation of the Ca(v)1.2 calcium channel connects gene transcription in pancreatic beta-cells through the kinase c-Jun N-terminal protein kinase and transcription factor AP-1.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Cell Biology
Ololade Ogunsina, Richa Banerjee, Luz A. A. Knauer, Ying Yang
Summary: The study found that FOXO1 inhibits the formation of lymphatic valves, making it a potential target for treating congenital lymphedema. The use of the FOXO1 inhibitor AS1842856 in both in vitro and in vivo models resulted in a significant increase in the expression levels of valve-forming genes and activation of beta-catenin, promoting lymphatic valve formation. These findings suggest that pharmacological inhibition of FOXO1 could be an effective strategy for resolving valve defects in congenital lymphedema.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Vipasha Gautam, Kajal Rawat, Arushi Sandhu, Puja Kumari, Neha Singh, Lekha Saha
Summary: Despite years of research, epilepsy still affects a large number of individuals and current drugs do not target the underlying pathophysiology. Recent studies focus on various signaling pathways such as mTOR and Wnt to understand epileptogenesis. Further investigation is needed to explore the potential of targeting these pathways for future epilepsy therapies.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Kelly S. Harrison, Clinton Jones
Summary: Research has shown that beta-catenin promotes productive infection of HSV-1 by enhancing the Wnt signaling pathway. Antagonists of the Wnt signaling pathway, KYA1797K and iCRT14, significantly inhibit productive infection of HSV-1 in neuronal and kidney cells.
ANTIVIRAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ryosuke Wakabayashi, Yasunao Hattori, Shigekuni Hosogi, Yuki Toda, Kazuyuki Takata, Eishi Ashihara
Summary: Compound 31 was identified as an inhibitor of the Wnt/beta-catenin pathway, showing potential as a novel anti-AML agent by suppressing TCF activity, reducing AML cell proliferation, inducing apoptosis, and synergistically inhibiting cell growth when combined with idarubicin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Chengming Zhang, Zhiyi Liu, Xiaotian Wang, Bin Zhang, Licheng Cui, Qinghe Hu, Bin Hu, Kuan Cao, Wengang Shan, Hengliang Shi, Renhao Wang
Summary: In this study, it was found that CTSK interacts with SIAH1 and inhibits its protein level, leading to increased proliferation of hepatocellular carcinoma (HCC) cells. CTSK mediates SIAH1 ubiquitination and degradation by promoting SIAH1 autoubiquitination and recruiting NEDD4 as an upstream ubiquitin ligase. These findings highlight the oncogenic role of CTSK in HCC progression.
Article
Biotechnology & Applied Microbiology
Ming Lei, Kangcheng Zhao, Wenbin Hua, Kun Wang, Shuai Li, Xinghuo Wu, Cao Yang
Summary: The study demonstrated that overexpression of c-Jun has a positive effect on the treatment of intervertebral disc degeneration, inhibiting the inflammatory response in vivo and potentially delaying the degeneration of the intervertebral disc.
Article
Multidisciplinary Sciences
Jake W. Willows, Gilian Gunsch, Emma Paradie, Magdalena Blaszkiewicz, Jeffrey R. Tonniges, Maria F. Pino, Steven R. Smith, Lauren M. Sparks, Kristy L. Townsend
Summary: Peripheral neuropathy, including axonal degeneration and demyelination, affects adipose tissues in obesity, diabetes, and aging. This study investigated the presence of demyelinating neuropathy in adipose tissue and found that adipose tissue contains myelinated and unmyelinated nerves populated by Schwann cells (SCs), which play a role in nerve regeneration. The study also showed that SCs in adipose tissue become dysregulated in diabetic peripheral neuropathy.
Article
Oncology
Himanshu Singh Saroha, Rohith Kumar Guddeti, Jasmine P. Jacob, Kiran Kumar Pulukuri, Prashanthi Karyala, Suresh B. Pakala
Summary: The expression of MORC2 is positively correlated with beta-catenin in breast cancer, and overexpression of MORC2 increases beta-catenin and its target genes. The target gene of beta-catenin, c-Myc, regulates the MORC2-beta-catenin signaling axis through a feedback mechanism. MORC2 regulates beta-catenin expression and function by modulating AKT phosphorylation, promoting proliferation and migration of breast cancer cells.
Article
Biotechnology & Applied Microbiology
Bronte Wen, Hyun Jun Jung, Lihe Chen, Fahad Saeed, Mark A. Knepper
Review
Mathematical & Computational Biology
Taban Eslami, Fahad Almuqhim, Joseph S. Raiker, Fahad Saeed
Summary: This article summarizes recent progress in using machine learning models for the diagnosis of ASD and ADHD, focusing on deep learning techniques. The future direction involves combining imaging techniques, such as MRI, with machine learning models to achieve and quantify the diagnosis of various mental disorders.
FRONTIERS IN NEUROINFORMATICS
(2021)
Article
Engineering, Chemical
Somphot Saoin, Chatikorn Boonkrai, Trairak Pisitkun, Chiraphat Kloypan, Sawitree Nangola
Summary: NGAL is considered a promising biomarker for early prediction of acute kidney injury. This study successfully expressed recombinant NGAL protein under a Tet-On inducible system, providing an efficient strategy for producing other recombinant proteins secreted from mammalian cells.
Letter
Oncology
Nut Pipatpanyanugoon, Nicha Wareesawetsuwan, Sunisa Prasopporn, Wannapan Poolex, Trairak Pisitkun, Worasak Kaewkong, Somponnat Sampattavanich, Siwanon Jirawatnotai
CANCER COMMUNICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Piyanoot Fonghem, Trairak Pisitkun, Kasem Rattanapinyopituk, Sirintra Sirivisoot, Anudep Rungsipipat
Summary: This study aimed to identify differential serum protein expressions of canine B- and T-cell lymphomas compared with healthy dogs using quantitative proteomics. The findings revealed potential lymphoma biomarkers in dogs and highlighted the need for further research with a larger sample size.
Article
Biochemical Research Methods
Nuttiya Kalpongnukul, Rungnapa Bootsri, Piriya Wongkongkathep, Pornchai Kaewsapsak, Chaiyaboot Ariyachet, Trairak Pisitkun, Naphat Chantaravisoot
Summary: This study investigated the abnormal mTORC2 signaling pathway in GBM cells and found that mTORC2 inhibition might be involved in DNA repair and cancer cell survival. Additionally, it was discovered that mTORC2 controls the phosphorylation of γH2AX to repair DNA. These findings provide important insights into the function of mTORC2 in oncogenic DNA damage response and offer potential mTORC2 treatments for brain cancer patients.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jiradej Makjaroen, Pornpimol Phuengmaung, Wilasinee Saisorn, Suwasin Udomkarnjananun, Trairak Pisitkun, Asada Leelahavanichkul
Summary: Lipopolysaccharide (LPS) tolerance, which reduces the immune response to repeated LPS stimulation, may contribute to sepsis-induced immune exhaustion. Macrophages with LPS tolerance showed decreased energy, reduced protein abundance, and down-regulated anti-viral genes. In asymptomatic mice, LPS tolerance was associated with higher viral abundance in the cecum and decreased expression of anti-viral genes, suggesting it could lead to viral reactivation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Sumonwan Chamsuwan, Chollada Buranakarl, Kris Angkanaporn, Thasinas Dissayabutra, Natthaya Chuaypen, Trairak Pisitkun, Nuttiya Kalpongnukul
Summary: This study found differences in urinary proteomic profiles between dogs with hypercalciuric CaOx urolithiasis and hypercalciuric stone-free dogs, suggesting that urinary proteomic profiles may serve as potential biomarkers for urinary tract injury in CaOx urolithiasis in dogs.
Article
Chemistry, Analytical
Pornchanok Punnoy, Tatiya Siripongpreda, Trairak Pisitkun, Nadnudda Rodthongkum, Pranut Potiyaraj
Summary: A novel lab-on-paper screening method using gold nanoparticles and laser desorption ionization-mass spectrometry (LDI-MS) was developed for rapid diagnosis of the SARS-CoV-2 Omicron BA.2 variant. The method allows for sensitive detection of SARS-CoV-2 antigen and can be used for quantitation of the antigen in saliva without the need for sample preparation. This platform could potentially be used for non-invasive diagnosis of the Omicron BA.2 variant.
Article
Computer Science, Information Systems
Muhammad Usman Tariq, Muhammad Haseeb, Mohammed Aledhari, Rehma Razzak, Reza M. Parizi, Fahad Saeed
Summary: Big Data Proteogenomics combines high-throughput Mass Spectrometry-based proteomics and Next Generation Sequencing-based genomics, facilitating the discovery of novel proteins in genomic and transcriptomic data. While there are numerous tools available for analyzing proteogenomic datasets, scalability issues have not been adequately addressed. Future workflows need to incorporate solutions for scalability to accommodate the increasing size of proteogenomic data.
Proceedings Paper
Computer Science, Information Systems
Taban Eslami, Fahad Saeed
2019 IEEE FIFTH INTERNATIONAL CONFERENCE ON BIG DATA COMPUTING SERVICE AND APPLICATIONS (IEEE BIGDATASERVICE 2019)
(2019)
Proceedings Paper
Computer Science, Artificial Intelligence
Oswaldo Artiles, Fahad Saeed
2019 IEEE INTERNATIONAL CONFERENCE ON BIG DATA (BIG DATA)
(2019)
Proceedings Paper
Biochemical Research Methods
Mohammed Aledhari, Shelby Joji, Mohamed Hefeida, Fahad Saeed
2019 IEEE INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICINE (BIBM)
(2019)
Proceedings Paper
Biochemical Research Methods
Muhammad Haseeb, Fahad Saeed
2019 IEEE INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICINE (BIBM)
(2019)
Article
Computer Science, Information Systems
Mohammed Aledhari, Rehma Razzak, Reza M. Parizi, Fahad Saeed