期刊
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
卷 303, 期 11, 页码 C1192-C1197出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpcell.00159.2012
关键词
AMPK; lipolysis; visceral fat; ATGL; perilipin
资金
- Natural Science and Engineering Research Council of Canada
- Canada Foundation for Innovation
- Ontario Research Fund
- Canadian Institutes of Health Research (CIHR)
- Ontario Ministry of Research and Innovation
- CIHR
- Canadian Diabetes Association
Gaidhu MP, Bikopoulos G, Ceddia RB. Chronic AICAR-induced AMP-kinase activation regulates adipocyte lipolysis in a time-dependent and fat depot-specific manner in rats. Am J Physiol Cell Physiol 303: C1192-C1197, 2012. First published October 10, 2012; doi:10.1152/ajpcell.00159.2012.-This study investigated the effects of chronic in vivo AMP-kinase activation with 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) on lipolysis in subcutaneous inguinal, epididymal, and retroperitoneal fat pads. Male Wistar rats received daily single intraperitoneal injections of either saline or AICAR (0.7 g/kg body wt) for a period of 8 wk. The fat pads were used either to isolate adipocytes and measure basal and catecholamine-stimulated lipolysis or to assess signaling steps of lipolysis after 4 and 8 wk of AICAR treatment. Blood was sampled weekly to measure nonesterified fatty acids (NEFAs). AICAR treatment reduced basal and catecholamine-stimulated lipolysis at week 4 in adipocytes from all fat depots. However, at week 8, catecholamine-induced lipolysis significantly increased in inguinal and retroperitoneal adipocytes. Interestingly, plasma levels of NEFAs were also decreased and subsequently increased at 4 and 8 wk, respectively. The lipolytic cascade of the inguinal fat pad was the most drastically affected by the treatment, since the phosphorylation and content of most proteins involved in lipolysis were consistently undetected in this tissue after 4 and 8 wk of AICAR treatment. The enhancement of catecholamine-induced lipolysis in inguinal and retroperitoneal adipocytes after 8 wk of AICAR treatment was accompanied by increased contents of adipose triglyceride lipase (ATGL) and perilipin A in these fat depots. In summary, despite depot-specific regulation of the lipolytic cascade, catecholamine-induced lipolysis in isolated adipocytes correlated well with plasma NEFA concentrations in the course of chronic AICAR-induced AMPK activation. The mechanisms underlying these effects also involved time-dependent and depot-specific regulation of hormone-sensitive lipase, ATGL, and perilipin.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据