Review
Biochemistry & Molecular Biology
Mounica Bandela, Patrick Belvitch, Joe G. N. Garcia, Steven M. Dudek
Summary: Cortactin (CTTN) is an actin-binding and cytoskeletal protein that plays a crucial role in regulating cellular processes, particularly in vascular endothelial cells. It undergoes post-translational modifications and interacts with other proteins to alter cytoskeletal structures and signaling mechanisms. Its function is important for maintaining barrier integrity and inhibiting vascular permeability in vascular endothelial cells. It also affects the pathophysiology of various lung disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Laura Locatelli, Mehdi Inglebert, Roberta Scrimieri, Priti Kumari Sinha, Gian Vincenzo Zuccotti, Paolo Milani, Lionel Bureau, Chaouqi Misbah, Jeanette A. M. Maier
Summary: This study demonstrates the different effects of high glucose on endothelial cells cultured in biomimetic microchannels. Macrovascular cells in 3D culture show remodeling of the cytoskeleton and alterations in cell shape, while microvascular cells undergo massive cytoskeletal disassembly and apoptosis under the same conditions. Furthermore, microvascular cells exhibit greater sensitivity to different flow regimens.
Article
Biochemistry & Molecular Biology
Pratap Karki, Boyoung Cha, Chen-Ou Zhang, Yue Li, Yunbo Ke, Kamoltip Promnares, Kozo Kaibuchi, Akihiko Yoshimura, Konstantin G. Birukov, Anna A. Birukova
Summary: SOCS1 negatively regulates inflammatory reaction and controls endothelial dysfunction by targeting microtubules, which is essential for its protective effects against endothelial permeability and inflammation.
Article
Pharmacology & Pharmacy
Nan Ma, Yohanes Cakrapradipta Wibowo, Phillip Wirtz, Doris Baltus, Thomas Wieland, Sepp Jansen
Summary: Tankyrase inhibitors have an impact on endothelial cell function by altering junctional dynamics, permeability, and migration. Mechanistically, tankyrase inhibition stabilizes angiomotin-like proteins 1 and 2, leading to the suppression of YAP1/TAZ nuclear translocation and TEAD-mediated transcription. Additionally, tankyrase inhibitors disrupt adherens junctions through RhoA-ROCK-mediated F-actin bundling. The actin cytoskeleton plays a role in the regulation of YAP1 and TAZ in endothelial cells.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Ophthalmology
Tapas Chandra Nag, Shilpa Gorla, Chiman Kumari, Tara Sankar Roy
Summary: The study found that damage to the pericytes in the choriocapillaris precedes endothelial changes in aging and age-related diseases. With increasing age, pericytes develop dark mitochondria, leading to damage to the capillary basal lamina and intermediate filaments. This ultimately results in the loss of the choriocapillaris, highlighting the importance of pericyte maintenance in the aging nervous system for future research.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Cell Biology
Mounica Bandela, Eleftheria Letsiou, Viswanathan Natarajan, Lorraine B. Ware, Joe G. N. Garcia, Sunit Singla, Steven M. Dudek
Summary: The study suggests that cigarette smoke or e-cigarette vapor-induced apoptosis in lung endothelial cells is related to the protein Cortactin (CTTN), with reduced levels of CTTN potentially increasing the risk of vascular damage and exacerbating the development of COPD.
Article
Cardiac & Cardiovascular Systems
Maria Bartosova, Conghui Zhang, Betti Schaefer, Rebecca Herzog, David Ridinger, Ivan Damgov, Eszter Levai, Iva Marinovic, Christoph Eckert, Philipp Romero, Peter Sallay, Akos Ujszaszi, Markus Unterwurzacher, Anja Wagner, Georg Hildenbrand, Bradley A. Warady, Franz Schaefer, Sotirios G. Zarogiannis, Klaus Kratochwill, Claus Peter Schmitt
Summary: This study investigated the impact of glucose degradation products (GDP) found in peritoneal dialysis fluids on accelerated vasculopathy development in chronic kidney disease (CKD) patients. The results indicated that high-GDP exposure led to increased cell death/apoptosis genes and decreased cell viability/survival genes in omental arterioles. Additionally, high-GDP exposure was associated with disrupted endothelial cell junction and cytoskeleton, leading to apoptosis in patients with CKD and on peritoneal dialysis.
CIRCULATION RESEARCH
(2021)
Article
Cell Biology
Na Zhang, Gang Wang, Gengyun Sun
Summary: This study revealed that IQGAP1 is significantly upregulated in LPS-induced models of acute lung injury, promoting barrier dysfunction and ICAM-1 upregulation in pulmonary microvascular endothelial cells by affecting the Rap1/Src signaling pathway. Knockdown of IQGAP1 can protect the barrier function of cells from LPS-induced damage.
CELLULAR SIGNALLING
(2021)
Review
Cell Biology
Joanna Kim, Olivia L. Mooren, Michael D. Onken, John A. Cooper
Summary: Septins play important roles in maintaining the integrity of endothelial cell monolayers. They are concentrated at cell-cell contacts and associate with plasma membrane lipids. Loss of septins disrupts cell-cell junctions and affects cell morphology and intercellular signaling.
Article
Chemistry, Analytical
Apresio K. Fajrial, Kun Liu, Yu Gao, Junhao Gu, Richard Lakerveld, Xiaoyun Ding
Summary: Characterization of physical biomarkers of cells through osmotic swelling can evaluate unique swelling dynamics of different cell types with high accuracy. This method can also detect changes in cellular mechanical properties due to cytoskeleton disruption, offering potential applications in both biological studies and clinical practice. Its simplicity makes swelling dynamics an invaluable label-free physical biomarker for cells.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Ghassan Bkaily, Danielle Jacques
Summary: The vascular endothelium is crucial in embryogenesis and aging, and it lines the blood vessels as a cell monolayer. It is recognized by the immune system as part of the body, so any abnormality in the endothelium exposes tissues to the immune system, leading to inflammation and vascular diseases like atherosclerosis. The endothelium's secretory role allows it to release vasoconstrictors, vasorelaxants, and cardio-modulatory factors that maintain proper circulatory system functioning. Adhesion molecules play a significant role in cardiovascular physiology and pathology by providing a seal for the monolayer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Shizheng Zhao, Shachuan Feng, Ye Tian, Zilong Wen
Summary: Hematopoietic stem cells (HSCs) and conventional endothelial cells (cECs) in the dorsal aorta (DA) display spatial separation and arise from a common precursor. The Etv2 dosage plays a crucial role in governing their fate specification.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Editorial Material
Hematology
Owen J. Tamplin
Summary: The study reveals a novel role of the chromatin remodeling factor Smarca during the development of HSPCs, as they emerge from the dorsal aorta hemogenic endothelium and transition to a fetal-like stage.
Article
Multidisciplinary Sciences
Nishkantha Arulkumaran, Mervyn Singer, Stefan Howorka, Jonathan R. R. Burns
Summary: In this study, the authors assemble DNA subunits into structural frameworks to support the formation of membrane-based protocells and prototissues, harnessing simplicity to create complexity.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Alla Revittser, Ivan Selin, Yuri Negulyaev, Vladislav Chubinskiy-Nadezhdin
Summary: The actin cytoskeleton is crucial for cell motility and migration, thus playing a key role in tissue repair. To enhance the migration rate of stem cells, researchers are exploring methods such as co-cultivation and preconditioning with biologically active compounds. Quantitative estimation of F-actin structure changes can be challenging, with fluorescence intensity measurements not always providing accurate results.
Article
Biochemistry & Molecular Biology
Katarzyna Niemirowicz-Laskowska, Joanna Mystkowska, Dawid Lysik, Sylwia Chmielewska, Grazyna Tokajuk, Iwona Misztalewska-Turkowicz, Agnieszka Z. Wilczewska, Robert Bucki
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Lukasz Suprewicz, Grazyna Tokajuk, Mateusz Ciesluk, Piotr Deptula, Teresa Sierpinska, Przemyslaw Wolak, Tomasz Wollny, Joanna Tokajuk, Stanislaw Gluszek, Ewelina Piktel, Robert Bucki
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Pharmacology & Pharmacy
Sylwia Joanna Chmielewska, Karol Sklodowski, Joanna Depciuch, Piotr Deptula, Ewelina Piktel, Krzysztof Fiedoruk, Patrycja Kot, Paulina Paprocka, Kamila Fortunka, Tomasz Wollny, Przemyslaw Wolak, Magdalena Parlinska-Wojtan, Paul B. Savage, Robert Bucki
Summary: By linking cationic steroid antimicrobial ceragenin CSA-131 with gold nanoparticles Au NPs, a new class of potent nanoantibiotics with strong bactericidal activity against multidrug-resistant bacteria has been developed, showing potential for effective eradication of MDR strains.
Article
Oncology
Ewelina Piktel, Ilona Oscilowska, Lukasz Suprewicz, Joanna Depciuch, Natalia Marcinczyk, Ewa Chabielska, Przemyslaw Wolak, Katarzyna Gluszek, Justyna Klimek, Piotr M. Zielinski, Michal T. Marzec, Paul B. Savage, Magdalena Parlinska-Wojtan, Robert Bucki
Summary: In this study, a novel nanosystem based on ceragenin CSA-131 attached to peanut-shaped gold nanoparticles demonstrated high effectiveness against ovarian cancer cells and limited toxicity. The use of non-spherical gold nanoparticles as a delivery platform for antineoplastics shows promise in enhancing therapeutic outcomes for ovarian malignancy.
Article
Pharmacology & Pharmacy
Karol Sklodowski, Sylwia Joanna Chmielewska, Joanna Depciuch, Piotr Deptula, Ewelina Piktel, Tamara Daniluk, Magdalena Zakrzewska, Michal Czarnowski, Mateusz Ciesluk, Bonita Durnas, Magdalena Parlinska-Wojtan, Paul B. Savage, Robert Bucki
Summary: Patients with chronic diabetes are more susceptible to infections.
Review
Microbiology
Tomasz Wollny, Tamara Daniluk, Ewelina Piktel, Urszula Wnorowska, Anna Buklaha, Katarzyna Gluszek, Bonita Durnas, Robert Bucki
Summary: Irritable bowel syndrome (IBS) is a common, chronic, functional disorder that impacts a large proportion of the world population, with its pathophysiology not fully understood. Emerging evidence suggests that gut microbiota plays a significant role in the development of IBS, and therefore targeting microbial modulation may effectively enhance therapy.
Article
Biochemistry & Molecular Biology
Jakub Spalek, Przemyslaw Ociepa, Piotr Deptula, Ewelina Piktel, Tamara Daniluk, Grzegorz Krol, Stanislaw Gozdz, Robert Bucki, Slawomir Okla
Summary: This article describes the clinical application of different types of biomaterials in the field of otorhinolaryngology, focusing on their antimicrobial properties. Various biomaterials, such as ceramics and polymers, are widely used in ear, nose, and throat surgery. It is important to consider tissue restoration and prevention of microbial colonization when designing implant materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Joanna Tokajuk, Piotr Deptula, Sylwia J. Chmielewska, Karol Sklodowski, Zaneta A. Mierzejewska, Malgorzata Gradzka-Dahlke, Adam Tolstoj, Tamara Daniluk, Paulina Paprocka, Paul B. Savage, Robert Bucki
Summary: Ceragenin CSA-44 has been found to prevent biofilm formation on the surface of teeth and composite filling, as well as reduce the mass of established biofilm. This suggests that CSA-44 may serve as a potential agent in the development of new methods to combat oral pathogens and reduce the severity of oral infections.
Review
Biochemistry & Molecular Biology
Joanna Tokajuk, Piotr Deptula, Ewelina Piktel, Tamara Daniluk, Sylwia Chmielewska, Tomasz Wollny, Przemyslaw Wolak, Krzysztof Fiedoruk, Robert Bucki
Summary: The mechanisms for maintaining oral cavity homeostasis are influenced by environmental factors, and the oral microbiota and immune molecules play important roles. The LL-37 peptide is involved in maintaining microbial balance and is associated with the development of oral pathologies and systemic diseases.
Article
Biochemistry & Molecular Biology
Tomasz Wollny, Urszula Wnorowska, Ewelina Piktel, Lukasz Suprewicz, Grzegorz Krol, Katarzyna Gluszek, Stanislaw Gozdz, Janusz Kopczynski, Robert Bucki
Summary: The role of inflammation in bladder cancer development is still unclear. This study investigates the potential role of sphingosine-1-phosphate (S1P), a regulator of cellular processes in inflammation and cancer, and its effect on the expression of human cathelicidin (hCAP-18). The study suggests that S1P may promote bladder cancer development by stimulating the expression of hCAP-18.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Neurosciences
Mateusz Ciesluk, Katarzyna Pogoda, Ewelina Piktel, Urszula Wnorowska, Piotr Deptula, Robert Bucki
Summary: The evaluation of nanomechanical properties of tissues is increasingly important in both health and disease. These properties are influenced by the composition of the extracellular matrix and significantly impact tissue physiology and cell behavior. Pathophysiological changes result in significant alterations in tissue mechanics, potentially affecting drug efficacy, which is often overlooked in current in vitro experimental models.
Article
Chemistry, Physical
Dawid Lysik, Piotr Deptula, Sylwia Chmielewska, Robert Bucki, Joanna Mystkowska
Summary: This study investigated the biofilm formation of C. krusei and S. mutans on the surface of PLA and PCL immersed in artificial saliva. The mass and topography of the biofilm were assessed using microscopic observations and spectrometric measurements. The findings showed that the biofilm, especially S. mutans, promoted polymer degradation, highlighting the need for additional antimicrobial strategies.
Article
Biochemistry & Molecular Biology
Paulina Zeliszewska, Monika Wasilewska, Piotr Batys, Katarzyna Pogoda, Piotr Deptula, Robert Bucki, Zbigniew Adamczyk
Summary: This study investigated the adsorption kinetics of the SARS-CoV-2 spike protein subunit with the receptor binding domain on abiotic surfaces using a combination of sensitive methods. The results showed that the protein adsorbed on mica and silica surfaces in compact quasi-spherical aggregates under different pH conditions, and the experimental data were successfully interpreted in terms of theoretical results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Microbiology
Grzegorz Krol, Kamila Fortunka, Michal Majchrzak, Ewelina Piktel, Paulina Paprocka, Angelika Mankowska, Agata Lesiak, Maciej Karasinski, Agnieszka Strzelecka, Bonita Durnas, Robert Bucki
Summary: The usage of nanotechnology in fighting parasitic diseases is in the early stages of development. It brings hopes for targeting early parasitosis stages, compensating the lack of vaccines, and finding new treatment options for drug-resistant parasites. The physicochemical properties of nanomaterials, such as size, shape, surface charge, surfactants, and shell molecules, play important roles in designing metallic nanoparticles and specific nanosystems. The development of antiparasitic drugs and diagnostic tools using nanotechnology is expected to provide new and effective methods to improve prevention and reduce morbidity and mortality caused by these diseases.
Article
Medicine, Research & Experimental
Iwona Radziejewska, Malgorzata Borzym-Kluczyk, Katarzyna Leszczynska
Summary: Luteolin, a natural flavonoid, shows potential therapeutic effects on gastric cancer by reducing the expression levels of key factors like MUC1, sT antigen, and ADAM-17. It also induces the expression of inflammatory markers like IL-8 and cytokines such as IL-10 and NF-kappa B. The combination of luteolin and Helicobacter pylori seems to have a synergistic effect on suppressing gastric cancer progression.
BIOMEDICAL REPORTS
(2021)
