Article
Ophthalmology
Koji Sugioka, Teruo Nishida, Aya Kodama-Takahashi, Junko Murakami, Masahiko Fukuda, Osamu Matsuo, Shunji Kusaka
Summary: Urokinase-type plasminogen activator (uPA) plays a significant role in the degradation of extracellular matrix proteins, activation of growth factors, and regulation of cellular processes. Research on uPA-deficient mice has shown that uPA is important for corneal nerve regeneration and epithelial migration after injury.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Medicine, General & Internal
Irene Abicca, Daniela Giannini, Marta Gilardi, Anna Maria Roszkowska, Mariacristina Parravano, Fabiana Picconi, Simona Frontoni, Domenico Schiano-Lomoriello
Summary: Peripheral neuropathy can complicate diabetes mellitus (DM). In this study, corneal beading enlargement was observed in patients with T1DM compared to healthy controls, using a new algorithm for analysis. This finding suggests that identifying beading expansion in corneal nerve fiber using IVCM could be a useful tool in predicting peripheral neuropathy at an early stage.
FRONTIERS IN MEDICINE
(2022)
Article
Endocrinology & Metabolism
Maryam Ferdousi, Alise Kalteniece, Shazli Azmi, Ioannis N. Petropoulos, Georgios Ponirakis, Uazman Alam, Omar Asghar, Andrew Marshall, Catherine Fullwood, Maria Jeziorska, Caroline Abbott, Giuseppe Lauria, Catharina G. Faber, Handrean Soran, Nathan Efron, Andrew J. M. Boulton, Rayaz A. Malik
Summary: Corneal confocal microscopy (CCM) has shown to be more effective in identifying severe corneal nerve loss in patients with type 1 diabetes compared to type 2 diabetes. It also demonstrates good diagnostic accuracy for diabetic peripheral neuropathy (DPN). Furthermore, the risk factors for a reduction in corneal nerve fiber length differ between type 1 and type 2 diabetes.
Article
Immunology
Xiongfeng Pan, Atipatsa C. Kaminga, Sanjay Kinra, Shi Wu Wen, Hongying Liu, Xinrui Tan, Aizhong Liu
Summary: This study summarized the associations of different chemokines with T1DM, revealing that circulating concentrations of CCL5 and CXCL1 were significantly higher in T1DM patients compared to controls. Circulating CCL5 ranked highest among all the chemokines investigated in T1DM.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Luisa H. Colorado, Lauren Beecher, Nicola Pritchard, Khaled Al Rashah, Cirous Dehghani, Anthony Russell, Rayaz A. Malik, Nathan Efron, Katie Edwards
Summary: Time-lapsed in vivo corneal confocal microscopy (IVCCM) revealed an increase in whorl region dendritic cell (DC) density in individuals with type 1 diabetes (T1D), but no changes in other DC sub-types or cell dynamics. Furthermore, there were correlations between DC density and lipid parameters, as well as between DC dynamics and renal function.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Uazman Alam, Georgios Ponirakis, Omar Asghar, Ioannis N. Petropoulos, Shazli Azmi, Maria Jeziorska, Andrew Marshall, Andrew J. M. Boulton, Nathan Efron, Rayaz A. Malik
Summary: Accurate identification of the high-risk group for diabetic peripheral neuropathy progression in diabetic patients is crucial, and corneal confocal microscopy is an effective method for this purpose.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Alexander Lucius, Sirjan Chhatwal, Monika Valtink, Peter S. Reinach, Aruna Li, Uwe Pleyer, Stefan Mergler
Summary: Tear film hyperosmolarity induces dry eye syndrome through TRPV1 activation. L-carnitine, a therapeutic agent, protects against hypertonicity-induced response. This study shows that L-carnitine inhibits TRPV1 activation by blocking cell volume shrinkage induced by hypertonicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Annika Vestergaard Kvist, Mohamad I. Nasser, Peter Vestergaard, Morten Frost, Andrea M. Burden
Summary: A study in Denmark from 1997 to 2017 found that the incidence rates of fractures were higher in patients with type 1 and type 2 diabetes compared to those without diabetes, except for foot fractures. The incidence of hip fractures decreased by 35.2%, 47.0%, and 23.4% in patients with type 1 diabetes, type 2 diabetes, and without diabetes, respectively, over the last 5 years. However, vertebral fractures increased by 14.8%, 18.5%, and 38.9% in the same groups. After age adjustment, patients with type 1 diabetes still had a higher risk of fractures compared to those without diabetes, while patients with type 2 diabetes had a risk similar to those without diabetes.
Article
Endocrinology & Metabolism
Joshua Machet, Mijeong Park, Alexander Richardson, Michael Carnell, Margaret A. Mouat, Nicola J. Smith, Nigel Turner, Blake J. Cochran, Kerry-Anne Rye, Nick Di Girolamo
Summary: The quantification of corneal basal nerve parameters by in vivo confocal microscopy has potential in identifying early manifestations of diabetic peripheral neuropathy. However, its diagnostic accuracy is affected by neuron length and other parameters. This study analyzed morphological changes associated with type 2 diabetes progression using high-resolution images of murine corneal nerves.
JOURNAL OF DIABETES INVESTIGATION
(2023)
Article
Ophthalmology
Diana Patricia Amador-Munoz, Valentina Conforti, Luisa Marina Matheus, Nicolas Molano-Gonzalez, Cesar Payan-Gomez
Summary: Patients with both type 1 and type 2 diabetes mellitus have a reduction in corneal endothelial cell density and an increase in corneal thickness. The impact is more significant in type 1 diabetes patients than type 2 diabetes patients. Aging and disease duration also play a role in these corneal changes.
Article
Multidisciplinary Sciences
Yoo Jin Kim, Tae Gi Kim
Summary: The study aimed to evaluate differences in corneal endothelial cell morphology and thickness between patients with and without type 2 diabetes. It found that diabetic patients had lower endothelial cell density, hexagonality, higher cell size variation, and thicker corneal thickness compared to non-diabetic patients. These differences were more pronounced in patients with longer disease duration and higher HbA1c levels. The duration of diabetes was inversely correlated with endothelial cell density.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yu-Hung Lai, Po-Yen Lee, Chi-Yu Lu, Yu-Ru Liu, Shu-Chi Wang, Ching-Chih Liu, Yo-Chen Chang, Yung-Hsiang Chen, Chia-Cheng Su, Chia-Yang Li, Po-Len Liu
Summary: This study found that TSP1 protects HCE cells from hypoxia-induced damages by regulating exosomal protein expression to promote cell migration, cell-cell adhesion, and extracellular matrix remodeling. TSP1 also improves hypoxia-induced paraptosis in HCE cells by promoting wound healing and remodeling.
Article
Endocrinology & Metabolism
Xuan Chen, Alison H. Affinati, Yungchun Lee, Adina F. Turcu, Norah Lynn Henry, Elena Schiopu, Angel Qin, Megan Othus, Dan Clauw, Nithya Ramnath, Lili Zhao
Summary: This study found that the risk of developing ICI-T1DM is associated with the type of ICI therapy, patient age, and preexisting non-T1DM diabetes. The development of ICI-T1DM does not seem to significantly impact patient survival.
Article
Biochemistry & Molecular Biology
Fawzia Bardag-Gorce, Alissa Diaz, Robert Niihara, Jeremy Stark, Daileen Cortez, Alexander Lee, Richard Hoft, Yutaka Niihara
Summary: This study investigated the expression of ALDHs in rabbit corneas with LSCD and found that grafting with CAOMECS to reconstruct corneal epithelium can reduce the expression levels of ALDH enzymes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Shaishav Dhage, Maryam Ferdousi, Safwaan Adam, Jan Hoong Ho, Alise Kalteniece, Shazli Azmi, Uazman Alam, Georgios Ponirakis, Ioannis Petropoulos, Andrew J. Atkinson, Andrew Marshall, Maria Jeziorska, Handrean Soran, Rayaz A. Malik
Summary: The study demonstrates the worsening of diabetic neuropathy across various measures, especially with corneal confocal microscopy (CCM), despite improvements in HbA1c and LDL-C. It further supports the utility of CCM as a rapid, non-invasive surrogate measure of diabetic neuropathy.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Ellen Heck, Kristel Gruslin, Valerie Corder, W. Matthew Petroll, Jill Urban
CELL AND TISSUE BANKING
(2020)
Article
Ophthalmology
Brock Hansen, Preston H. Blomquist, Peter Ririe, Severin Pouly, Chan Nguyen, W. Matthew Petroll, James P. McCulley
JOURNAL OF CATARACT AND REFRACTIVE SURGERY
(2020)
Article
Ophthalmology
William H. Waldrop, Matthew J. Gillings, Danielle M. Robertson, W. Matthew Petroll, V. Vinod Mootha
Article
Biophysics
Daniel P. Maruri, Miguel Miron-Mendoza, Pouriska B. Kivanany, Joshua M. Hack, David W. Schmidtke, W. Matthew Petroll, Victor D. Varner
BIOPHYSICAL JOURNAL
(2020)
Review
Ophthalmology
Caroline E. Murchison, W. Matthew Petroll, Danielle M. Robertson
Summary: Corneal crosslinking is an approved therapy to prevent progression of corneal ectasia in keratoconus patients. The risk of infection after epithelial-off crosslinking is low, with bacterial infections being the most common. Close monitoring and immediate postoperative care are important for these patients.
JOURNAL OF CATARACT AND REFRACTIVE SURGERY
(2021)
Article
Cell Biology
Ellen Heck, Valerie Corder, Jill Urban, Matthew Petroll, William Timmons, Dwight Cavanaugh
Summary: The combination of serologic testing and Donor Risk Assessment Interview (DRAI) has made tissue transplantation a frequent and safe modality. A study found that DRAI screening defers most, but not all, potential serologically reactive donors, highlighting the need for both screening and testing to ensure transplantation safety.
CELL AND TISSUE BANKING
(2021)
Article
Ophthalmology
Cameron D. Ward, Caroline E. Murchison, W. Matthew Petroll, Danielle M. Robertson
Summary: This study compared the intra- and interobserver repeatability of the LacryDiag Ocular Surface Analyzer and the OCULUS Keratograph 5M, finding good correlation but poor agreement between the devices for tear-related parameters. Both the repeatability of testing devices and the use of multiple outcome measures are crucial for diagnosing and monitoring patients with dry eye disease.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2021)
Article
Ophthalmology
Krithika S. Iyer, Daniel P. Maruri, Kara E. Peak, David W. Schmidtke, W. Matthew Petroll, Victor D. Varner
Summary: This study investigated the effects of substrate stiffness on the proliferation and motility of primary corneal keratocytes treated with PDGF-BB. The results showed that stiffer substrates promoted cell proliferation in the presence of PDGF-BB, but did not affect cell migration.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Ophthalmology
Shyon Parsa, Alejandro Rodriguez, Danielle M. Robertson, Robert Wayne Bowman, Walter Matthew Petroll
Summary: The goal of this study was to evaluate the temporal and spatial pattern of wound healing following UV corneal cross-linking (CXL) using 3-dimensional (3-D) confocal imaging in vivo. The study found different wound healing responses in two patients, and 3-dimensional confocal imaging can help identify unusual wound healing responses that are not clinically observed.
EYE & CONTACT LENS-SCIENCE AND CLINICAL PRACTICE
(2022)
Article
Cell Biology
Daniel P. Maruri, Krithika S. Iyer, David W. Schmidtke, W. Matthew Petroll, Victor D. Varner
Summary: After injury and refractive surgery, corneal wound healing can lead to fibrotic response, which is related to the differentiation of corneal keratocytes in response to TGF-beta 1. This study investigates how mechanosensing via focal adhesions regulates the stiffness-dependent myofibroblast differentiation of corneal keratocytes treated with TGF-beta 1.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tina B. McKay, Pouriska B. Kivanany, Sarah E. Nicholas, Okhil K. Nag, Michael H. Elliott, W. Matthew Petroll, Dimitrios Karamichos
Summary: This study evaluated the effects of topical application of quercetin on corneal scar development using animal models and in vitro analysis. The results suggest that quercetin may inhibit corneal scarring, but further studies are needed to validate its efficacy and safety for clinical applications.
Article
Engineering, Biomedical
Kevin H. Lam, Tarik Z. Shihabeddin, Jacob A. Awkal, Alex M. Najjar, Miguel Miron-Mendoza, Daniel P. Maruri, Victor D. Varner, W. Matthew Petroll, David W. Schmidtke
Summary: During corneal wound healing, corneal keratocytes respond to multiple cues simultaneously, integrating both physical and soluble signals. The presence of adsorbed fibronectin initially activates keratocytes, resulting in changes in cell shape, stress fiber formation, and expression of alpha-smooth muscle actin (alpha-SMA). However, when simultaneous exposure to adsorbed fibronectin and soluble platelet-derived growth factor-BB (PDGF-BB) occurs, keratocytes elongate and show reduced stress fibers and alpha-SMA expression. Furthermore, when plated on aligned collagen fibrils, keratocytes elongate in the direction of the fibrils in the presence of PDGF-BB.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Ophthalmology
W. Matthew Petroll, Miguel Miron-Mendoza, Yukta Sunkara, Hikaru R. Ikebe, Nishith R. Sripathi, Hajar Hassaniardekani
Summary: Previous studies have shown that UV cross-linking (CXL) increases stromal stiffness and alters extracellular matrix (ECM) microstructure. In this study, CXL was combined with phototherapeutic keratectomy (PTK) to investigate its effects on keratocyte differentiation and patterning, as well as fibroblast migration and myofibroblast differentiation. The results demonstrated that CXL prevents fibrosis on top of the stroma and leads to long term increases in stromal thickness in rabbits.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Ophthalmology
W. Matthew Petroll, Victor D. Varner, David W. Schmidtke
EXPERIMENTAL EYE RESEARCH
(2020)
Article
Biotechnology & Applied Microbiology
Miguel Miron-Mendoza, Dalia Vazquez, Nerea Garcia-Ramila, Hikaru R. Ikebe, W. Matthew Petroll
BIOENGINEERING-BASEL
(2020)