期刊
AMERICAN JOURNAL OF PATHOLOGY
卷 181, 期 5, 页码 1847-1861出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ajpath.2012.08.007
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资金
- Ministry of Health, Labor, and Welfare, Japan
- A3 foresight program from the Japan Society for Promotion of Science
- Japan Society for Promotion of Science
- Japanese Foundation for Research and Promotion of Endoscopy Grant
- Princess Takamatsu Cancer Research Fund [09-24119]
- Grants-in-Aid for Scientific Research [23501261, 23590920, 22790655, 23791283, 23659658, 24650632, 23240129] Funding Source: KAKEN
The concept of the CpG island methylator phenotype (CIMP) in colorectal cancer (CRC) is widely accepted, although the timing of its occurrence and its interaction with other genetic defects are not fully understood. Our aim in this study was to unravel the molecular development of CIMP cancers by dissecting their genetic and epigenetic signatures in precancerous and malignant colorectal lesions. We characterized the methylation profile and BRAF/KRAS mutation status in 368 colorectal tissue samples, including precancerous and malignant lesions. In addition, genome-wide copy number aberrations, methylation profiles, and mutations of BRAF, KRAS, TP53, and PIR3CA pathway genes were examined in 84 colorectal lesions. Genome-wide methylation analysis of CpG islands and selected marker genes revealed that CRC precursor lesions are in three methylation subgroups: CIMP-high, CIMP-low, and CIMP-negative. Interestingly; a subset of GIMP-positive malignant lesions exhibited frequent copy number gains on chromosomes 7 and 19 and genetic defects in the AKT/PIK3CA pathway gems. Analysis of mixed lesions containing both precancerous and malignant components revealed that most aberrant methylation is acquired at the precursor stage, whereas copy number aberrations are acquired during the progression from precursor to malignant lesion. Our integrative genomic and epigenetic analysis suggests early onset of CIMP during CRC development and indicates a previously unknown CRC development pathway in which epigenetic instability associates with genomic alterations. (Am J Pathol 2012, 181:1847-1861; http://dx.doi.org/10.1016/j.ajpath.2012.08.007)
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