Article
Biotechnology & Applied Microbiology
Ying Fang, Qianqian Yang
Summary: This study found that the upregulation of lncRNA SPINT1-AS1 promotes the progression of colorectal cancer by inhibiting miR-214 and increasing HDGF expression.
Article
Food Science & Technology
Maram Morjen, Wassim Moslah, Imen Touihri-Baraketi, Najet Srairi-Abid, Jose Luis, Naziha Marrakchi, Jed Jebali
Summary: This study successfully cloned and expressed the first active anti-oncogenic snake venom Kunitz-type protease inhibitor, PIVL. It inhibits the growth of glioblastoma cells and blocks angiogenesis. Both native PIVL and recombinant PIVL modulate the PI3/AKT and MAPK signaling pathways.
Article
Fisheries
Chantaka Wongdontri, Phattarunda Jaree, Kunlaya Somboonwiwat
Summary: The low molecular weight protein KuSPI plays a role in modulating various biological processes. In Penaeus monodon, the PmKuSPI gene is highly expressed in WSSV-infected shrimp and is regulated by a conserved microRNA, pmo-miR-bantam. Silencing the PmKuSPI gene in healthy shrimp resulted in delayed mortality and reduced hemocyte number and WSSV copies in infected shrimp. The pmo-miR-bantam regulates PmKuSPI post-transcriptionally and affects shrimp susceptibility to WSSV infection through hemocyte homeostasis.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Navodipa Bhattacharya, Nivedita Kolvekar, Sukanta Mondal, Angshuman Sarkar, Dibakar Chakrabarty
Summary: Vipegrin, a 6.8 kDa Kunitz-type serine proteinase inhibitor, has been purified from Russell's viper venom. It inhibits the catalytic activity of trypsin and shows disintegrin-like properties, inhibiting collagen and ADP-induced platelet aggregation. Vipegrin is cytotoxic to MCF7 breast cancer cells and disrupts their adhesion and invasive abilities.
Article
Immunology
Lizhi Pang, Madeline Dunterman, Songlin Guo, Fatima Khan, Yang Liu, Erfan Taefi, Atousa Bahrami, Changiz Geula, Wen-Hao Hsu, Craig Horbinski, Charles David James, Peiwen Chen
Summary: The protein TFPI2 plays a critical role in connecting glioblastoma stem cells and immunosuppressive microglia, promoting tumor growth and immunosuppression. Inhibiting the TFPI2-CD51-STAT6 signaling axis activates T cells and enhances the effectiveness of anti-PD1 therapy in glioblastoma mouse models. TFPI2 is positively correlated with stemness, microglia abundance, immunosuppression, and poor prognosis in human glioblastoma.
Article
Biochemistry & Molecular Biology
Livia de Moraes Bomediano Camillo, Graziele Cristina Ferreira, Adriana Feliciano Alves Duran, Flavia Ribeiro Santos da Silva, Wanius Garcia, Ana Ligia Scott, Sergio Daishi Sasaki
Summary: rBmTI-A is a recombinant serine protease inhibitor belonging to the Kunitz-BPTI family, showing inhibitory activities against bovine trypsin, human plasma kallikrein, human neutrophil elastase, and plasmin. It has two inhibitory domains with six cysteines each, forming three disulfide bonds for high structural stability. Experimental results suggest its protective potential against pulmonary emphysema, anti-inflammatory properties, and potent inhibitory activity on in vitro vessel formation, while circular dichroism data confirmed its thermostability and secondary structure.
Article
Biochemistry & Molecular Biology
Sandra Weiss, Ramona S. Adolph, Kristian Schweimer, Andrea DiFonzo, Marat Meleshin, Mike Schutkowski, Clemens Steegborn
Summary: Sirt1, an important protein deacylase, is regulated by the interaction with the regulator protein AROS. AROS acts as a competitive inhibitor, inhibiting the activity of Sirt1. The findings provide molecular insights into the physiological regulation of Sirt1 and potential opportunities for drug development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kazuhiro Yamamoto, Carsten Scavenius, Maria M. Meschis, Abdulrahman M. E. Gremida, Emilie H. Mogensen, Ida B. Thogersen, Simone Bonelli, Simone D. Scilabra, Anders Jensen, Salvatore Santamaria, Josefin Ahnstrom, George Bou-Gharios, Jan J. Enghild, Hideaki Nagase
Summary: This study investigates the role of low-density lipoprotein receptor-related protein 1 (LRP1) in the endocytosis process of articular cartilage. By analyzing the LRP1 interactome, the researchers identified numerous ligand candidates and confirmed their direct binding to LRP1. They also found that inhibition of LRP1-ligand interaction resulted in cell death and alterations in the secretome and transcriptional profile of human chondrocytes. The study highlights the specificity and extent of the chondrocyte LRP1 ligandome and identifies potential new therapeutic targets for osteoarthritis.
Article
Multidisciplinary Sciences
Stanley Borowicz, Daniel R. Principe, Matthew J. Dorman, Austin J. McHenry, Gautam Sondarva, Sandeep Kumar, Vijayalakshmi Ananthanarayanan, Patricia E. Simms, Ashley Hess, Ajay Rana
Summary: The loss of tumor suppressor gene HAI-1 leads to poor prognosis in NSCLC patients, characterized by increased M2 macrophages in the tumor microenvironment. This mechanism may contribute to an immunosuppressive M2-dominated TME through impaired M1 macrophage polarization. This suggests HAI-1 status could be informative for stratifying patients who may benefit from therapies targeting the HGF pathway, particularly as an adjuvant to ICI-based immunotherapy.
Article
Biochemistry & Molecular Biology
Kaito Harada, Takashi Yahata, Makoto Onizuka, Abd Aziz Ibrahim, Eri Kikkawa, Toshio Miyata, Kiyoshi Ando
Summary: The study highlights the inhibitory role of plasminogen activator inhibitor type-1 (PAI-1) in hematopoietic regeneration in bone marrow microenvironments with abundant bone marrow adipocytes (BMAs), suggesting that blocking PAI-1 activity could be a novel therapeutic approach. PAI-1 inhibitor treatment significantly increases stem cell factor concentration in obese patients, contributing to improved hematopoietic regeneration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Aline R. M. Lobba, Miryam Paola Alvarez-Flores, Melissa Regina Fessel, Marcus Vinicius Buri, Douglas S. S. Oliveira, Renata N. N. Gomes, Priscila S. S. Cunegundes, Carlos DeOcesano-Pereira, Victor D. D. Cinel, Ana M. M. Chudzinski-Tavassi
Summary: Amblyomin-X is a recombinant protein that inhibits FXa and has anticoagulant, antiangiogenic, and antitumor properties. It induces apoptosis in cancer cells and promotes tumor regression through non-hemostatic mechanisms. It has selective cytotoxicity towards tumor cells and has potential as a novel anticancer drug.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Pharmacology & Pharmacy
Kirstin Meiners, Prisca Hamm, Marcus Gutmann, Jan Niedens, Agnieszka Nowak-Krol, Salvador Pane, Tessa Luehmann
Summary: The study developed three polyethylene glycol (PEG)ylated tPA bioconjugates based on the recombinant human tPA drug Alteplase using site-specific conjugation strategies. These bioconjugates showed improved proteolytic activity, slower inhibition kinetics by PAI-1, and increased hydrodynamic size.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Yi-Zheng Zheng, Xiao-Ru Ji, Yun-Yang Liu, Shuai Jiang, Xiang-Ying Yu, Zhi-Ping Jia, Yue Zhao, Jun-Qiao Zhang, Jia-Li Zhang, Yi Kong
Summary: Five Kunitz-type peptides were identified from leeches, with the peptide WPK5-Mut showing strong inhibitory activity against FXIa. It has the potential to be a candidate for the development of an antithrombotic drug.
Article
Genetics & Heredity
Rui Tang, Xiaohong Lyu, Hong Li, Jinlyu Sun
Summary: This study investigates the relationship between genetic susceptibility and the polymorphisms of seven genes in Chinese patients with allergy disorders. The results suggest that the polymorphism of PAI-1 rs1799762 may be associated with cough performance in Chinese allergic disease patients.
FRONTIERS IN GENETICS
(2023)
Article
Medicine, Research & Experimental
Florence Boissiere-Michot, Caroline Mollevi, Volker Baecker, Evelyne Crapez, William Jacot
Summary: Urokinase plasminogen activator (uPA) and plasminogen activator inhibitor type 1 (PAI-1) have been identified as prognostic and predictive biomarkers in breast cancer, but their clinical use is limited due to the need for fresh-frozen or fresh tumor samples for quantification. This study compared the expression levels of PLAU and SERPINE1 mRNA (corresponding to uPA and PAI-1 proteins) using in situ hybridization with immunometric assay in breast tumor samples. The results showed that in situ hybridization cannot replace the immunometric assay for quantification of uPA and PAI-1 expression.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Bethasiwi Purbasari, Radha Madhyastha, Harishkumar Madhyastha, Queen Intan Nurrahmah, Masugi Maruyama, Yuichi Nakajima, Hiroaki Kataoka, Nozomi Watanabe
Summary: The study found that carbachol induces NLRP3 inflammasome activation through mAChR and NF-kappa B, while RA abolishes the inflammatory response. The co-administration of RA with cholinergic drugs may have the potential to prevent inflammatory responses during cholinergic medication.
PHARMACOLOGICAL REPORTS
(2022)
Article
Virology
Kosho Iwao, Takeshi Kawaguchi, Masatoshi Kimura, Chihiro Iwao, Mao Rikitake, Ayako Aizawa, Yumi Kariya, Motohiro Matsuda, Syunichi Miyauchi, Ichiro Takajo, Takumi Kiwaki, Tsuyoshi Fukushima, Hiroaki Kataoka, Tadaki Suzuki, Akihiko Okayama, Kunihiko Umekita
Summary: SFTS, a tickborne infectious disease caused by SFTSV, has a high mortality rate due to multiorgan failure. Reports have shown a correlation between SFTSV infection and mycosis, such as invasive pulmonary aspergillosis (IPA), with early diagnosis and treatment of IPA being crucial for SFTS patients.
Article
Cell Biology
Koji Yamamoto, Fumiki Yamashita, Makiko Kawaguchi, Aya Izumi, Takumi Kiwaki, Hiroaki Kataoka, Takeshi Kaneuji, Yoshihiro Yamashita, Tsuyoshi Fukushima
Summary: The study revealed that decreased expression of prostasin in oral squamous cell carcinoma is associated with more aggressive features and a poorer prognosis. Additionally, prostasin overexpression suppressed the proliferation and migration of OSCC cell lines in vitro, suggesting a potential role in tumor suppression.
Article
Biochemistry & Molecular Biology
Fumiki Yamashita, Takashi Kaieda, Takeshi Shimomura, Makiko Kawaguchi, Chen-Yong Lin, Michael D. Johnson, Hiroyuki Tanaka, Takumi Kiwaki, Tsuyoshi Fukushima, Hiroaki Kataoka
Summary: HAI-1, also known as SPINT1, acts as an inhibitor of matriptase and a chaperone to maintain the processing and localization of matriptase. The first Kunitz domain and the adjacent PKD domain-like internal domain of HAI-1 are essential for its chaperone function. HAI-1's chaperone function is primarily mediated by inhibiting undesired intracellular matriptase activity.
Letter
Pathology
Takumi Kiwaki, Mayumi Akaki Nagayasu, Naoshi Yamada, Yuki Kodama, Yoshiko Umekita, Tsuyoshi Fukushima, Hiroaki Kataoka, Hiroyuki Tanaka
PATHOLOGY INTERNATIONAL
(2022)
Article
Oncology
Aya Izumi, Koji Yamamoto, Makiko Kawaguchi, Fumiki Yamashita, Tsuyoshi Fukushima, Takumi Kiwaki, Hiroyuki Tanaka, Yoshihiro Yamashita, Hiroaki Kataoka
Summary: HAI-1 deficiency enhances pericellular protease activity, leading to the activation of growth factors associated with tumor progression in tongue squamous cell carcinoma.
Article
Cell Biology
Hiroyuki Nunoi, Peiyu Xie, Hideaki Nakamura, Yasuaki Aratani, Jun Fang, Toyoki Nishimura, Hiroaki Kataoka, Hiroshi Maeda, Makoto Matsukura
Summary: This study validates the efficacy of using polyethylene glycol-conjugated recombinant Fusarium spp. D-amino acid oxidase (PEG-fDAO) as a potential therapy for CGD-induced lung inflammation.
Review
Health Care Sciences & Services
Takumi Kiwaki, Hiroaki Kataoka
Summary: Three-dimensional culture methods and patient-derived organoids play a crucial role in the study of colorectal cancer, aiding in predicting patient sensitivity to chemotherapy and radiotherapy and guiding precision medicine.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Medicine, General & Internal
Mayumi Akaki Nagayasu, Tsuyoshi Fukushima, Fumitaka Matsumoto, Hideo Takeshima, Yuichiro Sato, Hiroaki Kataoka
Summary: Dura-attached supratentorial extra-axial ependymoma is a rare tumor subtype that is often misdiagnosed as a meningioma. This case report highlights the difficulty in accurately diagnosing this rare tumor using intraoperative histological and cytological examinations.
JOURNAL OF MEDICAL CASE REPORTS
(2022)
Article
Cell Biology
Masato Fujii, Takahiro Akioka, Shoichi Kimura, Takahiro Nagai, Takumi Kiwaki, Tsuyoshi Fukushima, Shoichiro Mukai, Toshiyuki Kamoto
Summary: In this study, dual inhibition of MET phosphorylation and pro-HGF activation using a MET inhibitor and HAI-2 overexpression was attempted to inhibit the growth of RCC cells. Both in vitro and in vivo experiments showed that combined treatment with MET-TKI and HAI-2 had the strongest inhibitory effect on MET phosphorylation and cell proliferation. This study suggests that combined treatment with MET-TKI and HAI-2 could be a candidate for a new strong therapy for RCC.
Article
Dermatology
Mayumi Akaki Nagayasu, Hiroyuki Tanaka, Kyoko Nogami, Yoji Kukita, Harumi Nakamura, Toru Motoi, Toshiaki Manabe, Hiroaki Kataoka
Summary: Cutaneous syncytial myoepithelioma is a rare tumor type characterized by nodular proliferation of oval to spindle-shaped tumor cells. The fusion of genes EWSR1 and PBX3 is commonly found in this type of tumor. We report a case with diagnostic difficulty and atypical immunohistochemical results, which were resolved by fluorescence in situ hybridization study to confirm EWSR1 rearrangement. Differential diagnoses and relevant literature are discussed.
JOURNAL OF CUTANEOUS PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Makiko Kawaguchi, Hiroaki Kataoka, Takumi Kiwaki, Liang Weiting, Sayaka Nagata, Kazuo Kitamura, Tsuyoshi Fukushima
Summary: Adrenomedullin (AM) is a peptide that attenuates intestinal mucosal inflammation and enhances mucosal regeneration by upregulating claudin-4 expression in primary-cultured murine intestinal epithelial cells and a mouse model of colitis. AM also reverses TNF alpha-mediated downregulation of claudin-4 and loss of cell-cell adhesion in a human intestinal epithelial cell line, indicating its potential in enhancing intestinal epithelial integrity.
Article
Medicine, General & Internal
Yoshiko Umekita, Kazumi Umeki, Fumiaki Kawano, Hiroyuki Tanaka, Hiroaki Kataoka
Summary: This article presents a case of papillary thyroid carcinoma with features of hyalinizing trabecular tumor and discusses its histopathological characteristics and diagnostic challenges. Although specific genetic alterations were not detected, this case may indicate a potential relationship between papillary thyroid carcinoma and hyalinizing trabecular tumor.
JOURNAL OF MEDICAL CASE REPORTS
(2023)
