期刊
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
卷 198, 期 1, 页码 -出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.ajog.2007.05.044
关键词
bacterial culture; placenta; polymerase chain reaction
资金
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [P30HD018655] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [U01NS040069] Funding Source: NIH RePORTER
- NICHD NIH HHS [P30-HD-18655] Funding Source: Medline
- NINDS NIH HHS [1 U01 NS 40069-01A2] Funding Source: Medline
OBJECTIVE: The objective of the study was to quantify and identify aerobic and anaerobic bacteria as well as Mycoplasma and Ureaplasma in the chorionic parenchyma. STUDY DESIGN: A sample of the chorionic parenchyma from neonates delivered between 23-27 completed weeks was cultured and tested by polymerase chain reaction (PCR) methods using universal bacterial primers for the presence of bacteria and mycoplasmas. RESULTS: The culture positive rate was higher for vaginal deliveries (333/ 489; 68%) than for cesarean sections (363/876; 41%). Thirty percent of all culture-positive samples had only aerobic bacteria, 21% of the samples had only anaerobic bacteria, and 9% of the samples had only Mycoplasma/ Ureaplasma. The mean concentration of Mycoplasma/Ureaplasma (4.00 +/- 1.11 log(10) CFU/g) was significantly higher (P < .001) than the total count of either aerobes (3.24 +/- 1.12 log(10) CFU/g) or anaerobes (2.89 +/- 0.99 log(10) CFU/g). Staphylococcus sp. and Corynebacterium sp. as well as organisms associated with bacterial vaginosis were the most frequently recovered. A PCR product was not detected from either randomly selected or known culture-positive samples. CONCLUSION: Approximately half of second-trimester placentas harbor organisms within the chorionic plate. The chorion parenchyma appears to harbor constituents that prevent the identification of bacterial deoxyribonucleic acid by PCR methods.
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