Article
Biochemical Research Methods
Niklas Philipp, Cedric K. Brinkmann, Jens Georg, Daniel Schindler, Bork A. Berghoff
Summary: DIGGER-Bac is a toolbox for designing and identifying seed regions and expressing synthetic sRNAs in bacteria. The SEEDling tool predicts optimal sRNA seed regions for efficient regulation of specific mRNA targets. The G-GArden tool assists with primer design for high-fidelity Golden Gate assembly of synthetic sRNA constructs.
Article
Biochemistry & Molecular Biology
Emilios Tassios, Christoforos Nikolaou, Nikolaos Vakirlis
Summary: Intergenic genomic regions have important regulatory and structural roles, and can potentially encode transmembrane domains. This property is found in intergenic regions of the yeast genome and is associated with the presence of transmembrane domains in evolutionarily young genes.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Neurosciences
Bianca M. van Kemenade, Gregor Wilbertz, Annalena Muller, Philipp Sterzer
Summary: The theory of predictive processing suggests that our brains interpret incoming sensory input by generating predictions based on internal models of the world, with conscious perception corresponding to the most probable model. Studies using functional magnetic resonance imaging have shown that early sensory cortices receive predictive feedback signals reflecting the contents of conscious perception.
HUMAN BRAIN MAPPING
(2022)
Article
Biochemistry & Molecular Biology
Marcia A. Hasenahuer, Alba Sanchis-Juan, Roman A. Laskowski, James A. Baker, James D. Stephenson, Christine A. Orengo, F. Lucy Raymond, Janet M. Thornton
Summary: In this study, constrained coding regions (CCRs) in the human genome were identified using DNA sequencing data from healthy control populations. These regions lack protein-changing variants and have been under constraint during human evolution. The distribution of CCRs was explored and their co-occurrence with different protein functional features was analyzed. Functional amino acids involved in DNA/RNA interactions, protein-protein contacts, and catalytic sites were found to be highly constrained. Surprisingly, linear motifs, linear interacting peptides, disorder-order transitions, and liquid-liquid phase separating regions also showed strong association with constraint for variability.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Virology
Alexis S. Chasseur, Gabrielle Trozzi, Celine Istasse, Astrid Petit, Perrine Rasschaert, Caroline Denesvre, Benedikt B. Kaufer, Luca D. Bertzbach, Benoit Muylkens, Damien Coupeau
Summary: Circular RNAs (circRNAs) have recently been rediscovered and found to be involved in gene regulation and cancer development. This study focused on viral circRNAs in Marek's disease virus (MDV) infections and identified a variety of viral circRNAs expressed during viral replication, latency, and reactivation. The circRNA expression mainly occurred in transcriptional units of major viral oncogenes and latency-associated transcripts (LATs). Additionally, a bioinformatics pipeline was established to identify circRNAs in other herpesviruses. This study provides important insights into MDV virulence genes and herpesviruses-mediated gene dysregulation.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Caroline Benz, Muhammad Ali, Izabella Krystkowiak, Leandro Simonetti, Ahmed Sayadi, Filip Mihalic, Johanna Kliche, Eva Andersson, Per Jemth, Norman E. Davey, Ylva Ivarsson
Summary: Specific protein-protein interactions are crucial for cell physiology, but many interactions are yet to be discovered. This study introduces an optimized peptide-phage display library that allows for the screening of numerous peptides in a single binding assay, aiding the discovery of SLiM-based interactions.
MOLECULAR SYSTEMS BIOLOGY
(2022)
Article
Ecology
Ni A. A. An, Jie Zhang, Fan Mo, Xuke Luan, Lu Tian, Qing Sunny Shen, Xiangshang Li, Chunqiong Li, Fanqi Zhou, Boya Zhang, Mingjun Ji, Jianhuan Qi, Wei-Zhen Zhou, Wanqiu Ding, Jia-Yu Chen, Jia Yu, Li Zhang, Shaokun Shu, Baoyang Hu, Chuan-Yun Li
Summary: Human de novo genes can originate from neutral long non-coding RNA (lncRNA) loci and are evolutionarily significant. The distinctive U1 elements and RNA splice-related sequences drive the origin of de novo genes from lncRNA loci and distinguish them from lncRNAs. The functional new genes acquire functions along with the achievement of their coding potential.
NATURE ECOLOGY & EVOLUTION
(2023)
Review
Microbiology
Brice Felden, Yoann Augagneur
Summary: Bacterial gene expression is controlled by a complex system of molecules, with small regulatory RNAs (sRNAs) emerging as key players alongside transcription factors. sRNAs influence various biological processes and can act intracellularly or even in extracellular vesicles, impacting host cell interactions. Studies suggest that sRNA-mediated mechanisms are more diverse than previously thought, beyond traditional classifications.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Ru Chen, Juan Wu, Chang Lu, Ting Yan, Yu Qian, Huiqing Shen, Yujing Zhao, Jianzhen Wang, Pengzhou Kong, Xinri Zhang
Summary: CA extracted from cinnamon has shown antiviral and anti-tumor effects, including inhibiting proliferation, invasion, and migration, inducing apoptosis, and suppressing tumor growth in NSCLC. Through the ceRNA network, key genes SOCS1, BTG2, and BTK were identified as playing essential roles in CA against NSCLC.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
John R. Prensner, Oana M. Enache, Victor Luria, Karsten Krug, Karl R. Clauser, Joshua M. Dempster, Amir Karger, Li Wang, Karolina Stumbraite, Vickie M. Wang, Ginevra Botta, Nicholas J. Lyons, Amy Goodale, Zohra Kalani, Briana Fritchman, Adam Brown, Douglas Alan, Thomas Green, Xiaoping Yang, Jacob D. Jaffe, Jennifer A. Roth, Federica Piccioni, Marc W. Kirschner, Zhe Ji, David E. Root, Todd R. Golub
Summary: Noncanonical open reading frames have been shown to play essential roles in cancer cell function, encoding biologically active proteins that could serve as potential therapeutic targets. These findings suggest new avenues for the treatment of breast cancer.
NATURE BIOTECHNOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Nasim Ahmed, Muhammad Arslan Mahmood, Imran Amin, Shahid Mansoor
Summary: Researchers have identified small proteins encoded by Geminiviruses with specific cellular localizations and virulence functions, suggesting the need for further investigation into similar small proteins in other Geminiviruses.
TRENDS IN MICROBIOLOGY
(2021)
Article
Microbiology
Manuel Brenes-Alvarez, Agustin Vioque, Alicia M. Muro-Pastor
Summary: Heterocysts are specialized nitrogen-fixing cells in filamentous cyanobacteria, and NsiR1 is a noncoding RNA that regulates the expression of hetF and alr3234 genes to participate in heterocyst differentiation and commitment.
MICROBIOLOGY SPECTRUM
(2022)
Article
Neurosciences
Ally Dworetsky, Benjamin A. Seitzman, Babatunde Adeyemo, Maital Neta, Rebecca S. Coalson, Steven E. Petersen, Caterina Gratton
Summary: Recent studies have focused on individual differences in the functional network organization of the human brain and have utilized this information to probabilistically map common functional systems for improved group analyses. While these functional systems exhibit core regions, they vary in their higher-variability components and demonstrate good replication across datasets.
Review
Genetics & Heredity
Nicole A. Leypold, Michael R. Speicher
Summary: Research suggests that humans may share more genomic commonalities with other species than previously thought, including a significant fraction of conserved noncoding elements. As whole-genome sequencing becomes a standard procedure in genetic analyses, the interpretation of variations in these conserved elements is becoming increasingly necessary.
TRENDS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Nickolai A. Tchurikov, Elena S. Klushevskaya, Ildar R. Alembekov, Anastasiia S. Bukreeva, Antonina N. Kretova, Vladimir R. Chechetkin, Galina Kravatskaya, Yuri Kravatsky
Summary: Small noncoding RNAs derived from rDNA fragments are found throughout the human genome and play a role in gene expression regulation. These small RNAs, which are processed by the Microprocessor complex, correspond to specific regions of ribosomal RNAs and target certain genes involved in differentiation and development. The findings suggest that remnants of rDNA and small noncoding RNAs are involved in the regulation of gene expression in human cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Evgeny M. Zdobnov, Dmitry Kuznetsov, Fredrik Tegenfeldt, Mose Manni, Matthew Berkeley, Evgenia Kriventseva
Summary: OrthoDB provides evolutionary and functional annotations of orthologs for a vast number of organisms, with a wide coverage of species and rich data content. The user interface has been enhanced, and features three views on the data, as well as online tools for interactive exploration and data download.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biology
Pia U. Olafson, Serap Aksoy, Geoffrey M. Attardo, Greta Buckmeier, Xiaoting Chen, Craig J. Coates, Megan Davis, Justin Dykema, Scott J. Emrich, Markus Friedrich, Christopher J. Holmes, Panagiotis Ioannidis, Evan N. Jansen, Emily C. Jennings, Daniel Lawson, Ellen O. Martinson, Gareth L. Maslen, Richard P. Meisel, Terence D. Murphy, Dana Nayduch, David R. Nelson, Kennan J. Oyen, Tyler J. Raszick, Jose M. C. Ribeiro, Hugh M. Robertson, Andrew J. Rosendale, Timothy B. Sackton, Perot Saelao, Sonja L. Swiger, Sing-Hoi Sze, Aaron M. Tarone, David B. Taylor, Wesley C. Warren, Robert M. Waterhouse, Matthew T. Weirauch, John H. Werren, Richard K. Wilson, Evgeny M. Zdobnov, Joshua B. Benoit
Summary: This study utilizes high-quality genome sequencing and RNA-Seq analysis to delve into the biology of stable flies, focusing on genes related to reproduction, vision, chemosensation, immunity, and metabolic detoxification pathways. The results of the study will lay the groundwork for controlling stable fly infestations.
Article
Biochemistry & Molecular Biology
Stylianos E. Antonarakis, Ales Holoubek, Melivoia Rapti, Jesse Rademaker, Jenny Meylan, Justyna Iwaszkiewicz, Vincent Zoete, Callum Wilson, Juliet Taylor, Muhammad Ansar, Christelle Borel, Olivier Menzel, Katerina Kuzelova, Federico A. Santoni
Summary: Knobloch syndrome, typically linked to the COL18A1 gene, can also be associated with the PAK2 gene. A study on a specific family revealed that KNO2 syndrome in the family is dominantly caused by a deleterious variant in the PAK2 gene.
HUMAN MOLECULAR GENETICS
(2022)
Article
Multidisciplinary Sciences
Sina Rueeger, Christian Hammer, Alexis Loetscher, Paul J. McLaren, Dylan Lawless, Olivier Naret, Daniel P. Depledge, Sofia Morfopoulou, Judith Breuer, Evgeny Zdobnov, Jacques Fellay
Summary: This study identified significant associations between human genetic variation and EBV sequence variation, which could help dissect pathogenic mechanisms and suggest new therapeutic avenues.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
James M. Muthoka, Edward E. Salakpi, Edward Ouko, Zhuang-Fang Yi, Alexander S. Antonarakis, Pedram Rowhani
Summary: The study successfully detected Opuntia stricta in the ASAL of Kenya using Sentinel-2 multispectral sensors and ensemble machine learning classifiers, achieving high overall accuracies. The combination of topographic and vegetation indices improved classification accuracy.
Article
Green & Sustainable Science & Technology
Ushuki A. Amuyou, Yi Wang, Francis Bisong, Alexander S. Antonarakis
Summary: The rate of landcover change due to deforestation in tropical environments continues to rise, despite efforts such as the REDD+ policy to combat forest destruction. The study in Cross River State, Nigeria, found that financial payment and increased natural resources were perceived benefits of forest carbon protection. Household income was a key factor influencing support for forest carbon protection, with an increase in income attributed to a ban on logging.
Article
Biodiversity Conservation
Alexander S. Antonarakis, Stacy A. Bogan, Michael L. Goulden, Paul R. Moorcroft
Summary: Accurate descriptions of ecosystem composition using imaging spectrometry can significantly improve terrestrial biosphere model predictions of carbon, water, and energy fluxes. The study also found that the 2012-2015 Californian drought led to significant reductions in regional ecosystem productivity, with the most pronounced impact seen in high basal area mid-elevation forests.
GLOBAL CHANGE BIOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Alexander S. Antonarakis, Lucia Pacca, Andreas Antoniades
Summary: This study examines the impact of financial crises on deforestation rates globally, finding a reduction in deforestation during crisis periods. It also notes that financial crises have a larger effect on deforestation in low-income countries. The study emphasizes the need for governments to enhance sustainable forest management during crisis periods to maintain the benefits of reduced deforestation.
SUSTAINABILITY SCIENCE
(2022)
Article
Biochemical Research Methods
Silas Kieser, Evgeny M. Zdobnov, Mirko Trajkovski
Summary: This study created a comprehensive catalog of bacterial species commonly living in the gut of laboratory mice by analyzing all publicly available metagenomes. The catalog contains almost 42 thousand bacterial genomes from 1,573 species, of which 78.1% are uncultured. The researchers discovered that the microbial species in the gastrointestinal tracts of mice and humans are largely distinct.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Microbiology
Jaspreet S. Saini, Christel Hassler, Rachel Cable, Marion Fourquez, Francesco Danza, Samuele Roman, Mauro Tonolla, Nicola Storelli, Stephan Jacquet, Evgeny M. Zdobnov, Melissa B. Duhaime
Summary: This study examines the microbial community in Lake Cadagno and finds that there is persistent microbial growth in the lake with different types of microbes playing different roles in different layers of the lake. Viruses may play a role in regulating primary production.
Article
Biochemistry & Molecular Biology
Alina G. Mikhailova, Alina A. Mikhailova, Kristina Ushakova, Evgeny O. Tretiakov, Dmitrii Iliushchenko, Victor Shamansky, Valeria Lobanova, Ivan Kozenkov, Bogdan Efimenko, Andrey A. Yurchenko, Elena Kozenkova, Evgeny M. Zdobnov, Vsevolod Makeev, Valerian Yurov, Masashi Tanaka, Irina Gostimskaya, Zoe Fleischmann, Sofia Annis, Melissa Franco, Kevin Wasko, Stepan Denisov, Wolfram S. Kunz, Dmitry Knorre, Ilya Mazunin, Sergey Nikolaev, Jacques Fellay, Alexandre Reymond, Konstantin Khrapko, Konstantin Gunbin, Konstantin Popadin
Summary: The mutational spectrum of mtDNA differs from that of nuclear genome, and the variation in mtDNA mutational spectra between different organisms remains unclear. This study found that the frequency of A(H) > G(H) substitutions is twice as high in species with high generation length compared to those with low generation length, indicating that mtDNA in species with high generation length is more A(H) poor and G(H) rich. The rate of A(H) > G(H) substitutions is influenced by both species-specific generation length and position-specific time spent single-stranded during mtDNA replication. This suggests that A(H) > G(H) is a mitochondria-specific signature of oxidative damage associated with both aging and time spent single-stranded.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Dmitry Kuznetsov, Fredrik Tegenfeldt, Mose Manni, Mathieu Seppey, Matthew Berkeley, Evgenia Kriventseva, Evgeny M. Zdobnov
Summary: OrthoDB provides evolutionary and functional annotations of genes in a diverse range of organisms. It offers a comprehensive coverage of species diversity by sampling diverse organisms with high-quality genomic data. The update of underlying data and the scalability of the OrthoLoger software enhance the accuracy of ortholog delineation and allow mapping of novel gene sets. The web interface of OrthoDB has been further developed, providing a pairwise orthology view between any gene and other sampled species.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Environmental Sciences
Ushuki A. Amuyou, Yi Wang, Bisong Francis Ebuta, Chima J. Iheaturu, Alexander S. Antonarakis
Summary: This study estimated the regional distribution of aboveground biomass (AGB) in the Cross River State, Nigeria, using Sentinel-2 imagery and random forest machine learning algorithm. The results showed that the AGB estimation using random forest yielded favorable results compared to previous products, and the biomass map could be used for REDD+ monitoring and reporting.
Article
Ecology
Jaspreet S. Saini, Mose Manni, Christel Hassler, Rachel N. Cable, Melissa B. Duhaime, Evgeny M. Zdobnov
Summary: This study presents the first near-complete genome of a photosynthetic microbial eukaryote from the chemocline of Lake Cadagno. The findings suggest the cooperation of this microorganism with phototrophic sulfur bacteria via carbon, sulfur, and nitrogen metabolism, aiding their collective persistence in the lake's chemocline.
Article
Biochemistry & Molecular Biology
Adrian Altenhoff, Amos Bairoch, Parit Bansal, Delphine Baratin, Frederic Bastian, Jerven Bolleman, Alan Bridge, Frederic Burdet, Katrin Crameri, Jerome Dauvillier, Christophe Dessimoz, Sebastien Gehant, Natasha Glover, Kristin Gnodtke, Catherine Hayes, Mark Ibberson, Evgenia Kriventseva, Dmitry Kuznetsov, Frederique Lisacek, Florence Mehl, Tarcisio Mendes de Farias, Pierre-Andre Michel, Sebastien Moretti, Anne Morgat, Sabine Osterle, Marco Pagni, Nicole Redaschi, Marc Robinson-Rechavi, Kasun Samarasinghe, Ana-Claudia Sima, Damian Szklarczyk, Orlin Topalov, Vasundra Toure, Deepak Unni, Christian von Mering, Julien Wollbrett, Monique Zahn-Zabal, Evgeny Zdobnov
Summary: This paper introduces the SIB Swiss Institute of Bioinformatics and its 11 databases, which provide semantically enriched data according to the FAIR principles. It also discusses the Swiss Personalized Health Network initiative and how it uses semantic enrichment to manipulate data. Examples and the use of SPARQL query language are provided to show how the existing SIB knowledge graphs can address complex biological or clinical questions.
NUCLEIC ACIDS RESEARCH
(2023)