Article
Cell Biology
Hal E. Broxmeyer, Larry L. Luchsinger, Rona Singer Weinberg, Alexandra Jimenez, Emeline Masson Frenet, Wouter vant Hof, Maegan L. Capitano, Christopher D. Hillyer, Mark H. Kaplan, Scott Cooper, James Ropa
Summary: Long-term cryopreserved cord blood units retain similar numbers of hematopoietic stem and progenitor cells compared with fresh and recently cryopreserved units, and contain highly functional cells. These units demonstrate robust engraftment in mouse transplantation models, and gene program differences can potentially serve as markers for engraftment.
CELL REPORTS MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Ximena Bonilla, Ana Milena Lara, Manuela Llano-Leon, David A. Lopez-Gonzalez, David G. Hernandez-Mejia, Rosa Helena Bustos, Bernardo Camacho-Rodriguez, Ana-Maria Perdomo-Arciniegas
Summary: Umbilical cord blood is an important source of hematopoietic stem and progenitor cells, and recent research has focused on developing ex vivo expansion strategies for these cells. Co-culturing the cells with specific mesenchymal stromal cells has been found to significantly increase their cellular dose and regulate their proliferation capacity. Additionally, the secretory profile of umbilical cord blood-derived cells closely resembles that of bone marrow-derived cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Jianan Jiang, Jinhua Qin, Jisheng Li, Xiaosong Lin, Bowen Zhang, Zeng Fan, Lijuan He, Quan Zeng, Wen Yue, Min Zheng, Xuetao Pei, Yanhua Li
Summary: This study found that the small molecule Ricolinostat can promote the differentiation of megakaryocytic cells from HSPCs, increase the proliferation of megakaryocytic cells, and decrease the secretion of IL-8 and the expression of CXCR2, thereby improving the efficiency of megakaryocytic differentiation of HSPCs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Immunology
Lifei Hou, Koichi Yuki
Summary: CD11a plays a critical role in leukocyte arrest and immunological synapse formation, but its role in the bone marrow is not well studied. This research showed that CD11a is expressed on all subsets of hematopoietic stem and progenitor cells (HPSCs). CD11a deficiency enhances HSPCs activity under LPS stimulation, possibly by upregulating the production of IL-27 to promote cell proliferation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Serena Scala, Francesca Ferrua, Luca Basso-Ricci, Francesca Dionisio, Maryam Omrani, Pamela Quaranta, Raisa Jofra Hernandez, Luca Del Core, Fabrizio Benedicenti, Ilaria Monti, Stefania Giannelli, Federico Fraschetta, Silvia Darin, Elena Albertazzi, Stefania Galimberti, Eugenio Montini, Andrea Calabria, Maria Pia Cicalese, Alessandro Aiuti
Summary: This study found that hematopoietic stem/progenitor cells derived from mobilized peripheral blood and bone marrow have similar gene repair capabilities in ex vivo gene therapy for pediatric Wiskott-Aldrich syndrome. However, the peripheral blood group showed better treatment outcomes, with faster recovery and higher numbers of engrafted clones and gene corrections in the myeloid lineage, which are related to the higher amounts of primitive and myeloid progenitors in the peripheral blood-derived cells. This finding provides important references for the clinical interpretation of hematopoietic stem/progenitor cell transplantation outcomes.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
You Jung Hwang, Dong-Yeop Shin, Min-Jung Kim, Hyosun Jang, Soyeon Kim, Hyunwon Yang, Won Il Jang, Sunhoo Park, Sehwan Shim, Seung Bum Lee
Summary: This study found that SR1 could alleviate radiation-induced hematopoietic injury. It increased the survival rate and promoted the recovery of peripheral blood cell counts in irradiated mice. In vitro experiments also showed that SR1 increased the population of human hematopoietic stem/progenitor cells, reduced radiation-induced DNA damage and apoptosis. These results suggest that SR1 may be a potential radiomitigator for hematopoietic injury.
Article
Engineering, Biomedical
Wenjing Li, Haiwei Liang, Yanxiao Ao, Baixue Tang, Junyang Li, Ning Li, Jianwei Wang, Yanan Du
Summary: Hematopoietic stem cells (HSCs) have the ability to differentiate into all blood cells and immune cells. By simulating the bone marrow microenvironment, we designed degradable scaffolds to regulate the fate of HSCs. In vivo transplantation confirmed the importance of the scaffolds in maintaining hematopoietic function, providing a foundation for parameter design in 3D HSC culture systems.
Article
Hematology
Valentina Capo, Sara Penna, Ivan Merelli, Matteo Barcella, Serena Scala, Luca Basso-Ricci, Elena Draghici, Eleonora Palagano, Erika Zonari, Giacomo Desantis, Paolo Uva, Roberto Cusano, Lucia Sergi Sergi, Laura Crisafulli, Despina Moshous, Polina Stepensky, Katarzyna Drabko, Zuhre Kaya, Ekrem Unal, Alper Gezdirici, Giuseppe Menna, Marta Serafini, Alessandro Aiuti, Silvia Laura Locatelli, Carmelo Carlo-Stella, Ansgar S. Schulz, Francesca Ficara, Cristina Sobacchi, Bernhard Gentner, Anna Villa
Summary: Allogeneic hematopoietic stem cell transplantation is the preferred treatment for autosomal recessive osteopetrosis caused by TCIRG1 gene defects. However, some patients are not eligible due to disease severity. Utilizing circulating CD34(+) cells for gene therapy shows promise in overcoming limitations of stem cell harvest in osteopetrotic patients, potentially shaping future gene-based treatments for skeletal diseases.
Article
Biology
Daniel E. Morales-Mantilla, Bailee Kain, Duy Le, Anthony R. Flores, Silke Paust, Katherine Y. King
Summary: This study suggests that hematopoietic stem and progenitor cells (HSPCs) have the potential to be used as a new therapeutic strategy for sepsis. Infusion of HSPCs in mice with sepsis resulted in improved survival rates and reduced inflammation. These findings highlight the important immunomodulatory role of HSPCs and their potential for treating sepsis.
Article
Oncology
Qihao Sun, Yan Fu, Xuejun Zhu, Wen-Song Tan, Haibo Cai
Summary: This study demonstrated that inhibition of the NF-kappa B pathway improved glycolysis and limited ROS production in HSPCs, promoting their ex vivo expansion and maintenance of functionality.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Developmental Biology
Suwei Gao, Yifan Zhang, Feng Liu
Summary: For a long time, it was believed that self-renewing and multipotent hematopoietic stem cells (HSCs) were the main contributors to both embryonic and adult hematopoiesis. However, recent studies have shown that native hematopoiesis is not solely driven by hierarchical differentiation of HSCs, but rather by hematopoietic progenitor cells (HPCs). These findings help redefine our understanding of the cellular origins of embryonic and adult hematopoiesis and highlight the differences in revealing HSC potential versus HSC fate using different approaches.
Article
Genetics & Heredity
Takahiro Sano, Koki Ueda, Keiji Minakawa, Tsutomu Mori, Yuko Hashimoto, Haruhiko Koseki, Yasuchika Takeishi, Kazuhiko Ikeda, Takayuki Ikezoe
Summary: This study generated Uhrf2(-/-) mice to investigate the role of UHRF2 deletion in hematopoiesis. The results showed that the absence of UHRF2 did not affect blood counts, bone marrow findings, and spleen weights. However, the proportions of Uhrf2(-/-) cells were decreased compared to Uhrf2(+/+) cells in competitive repopulation assays, indicating a potential regulatory role of UHRF2 in hematopoiesis.
Article
Engineering, Biomedical
Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao
Summary: This study demonstrates the successful generation of AGM-like hematopoietic system from human pluripotent stem cells through temporal manipulation of Wnt signaling. The generated hematopoietic progenitor cells resemble primary cord blood HSCs at the transcriptional level and exhibit lymphoid and myeloid potential in vitro. The cells also show the ability to home to a definitive hematopoietic site, rescue bloodless zebrafish, and engraft and repopulate in mouse recipients.
Review
Materials Science, Biomaterials
Jing Hong, Wenlong Zheng, Xiaoqiong Wang, Ying Hao, Guosheng Cheng
Summary: This review discusses recent advances in the use of biomedical polymer scaffolds to mimic bone marrow microenvironments for in vitro expansion of hematopoietic stem cells (HSCs), highlighting the potential future prospects in this field.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Cell & Tissue Engineering
Lina Wang, Yongjun Piao, Dongyue Zhang, Wenli Feng, Chenchen Wang, Xiaoxi Cui, Qian Ren, Xiaofan Zhu, Guoguang Zheng
Summary: This study demonstrates the negative role of Fbxw11 in the maintenance and repopulation capacity of HSPCs, and provides valuable transcriptomic references for HSPC homeostasis.
STEM CELL RESEARCH & THERAPY
(2022)
