期刊
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
卷 135, 期 4, 页码 566-571出版社
OXFORD UNIV PRESS INC
DOI: 10.1309/AJCPPVNUI3O9JHJW
关键词
Klebsiella pneumoniae carbapenemase; KPC; bla(KPC) genes; Modified Hodge test
类别
资金
- Department of Medical Education, Henry Ford Health System, Detroit, MI [9006]
Transfer of the bla(Kpc) genes encoding the Klebsiella pneumoniae carbapenemase (KPC) are increasingly responsible for emerging carbapenem resistance. The modified Hodge test (MHT) is recommended for the detection of KPC. We compared MHT with a real-time polymerase chain reaction (PCR) assay targeting common subtypes of bla(Kpc), using previously described forward and reverse primer sequences. The PCR product was detected using SYBR Green (Applied Biosystems, Foster City, CA) and confirmed by melt curve analysis. PCR was positive in 96% (52/54) of isolates that were MHT+, 90% (28/31) of MHT- isolates were PCR-, and the results were strongly correlated (P = .0001; Fisher exact test). The PCR assay is a sensitive, specific, and rapid test for detecting bla(KPC) genes. It could help optimize patient care by reducing the time taken to institute appropriate antimicrobial therapy and so help improve patient outcomes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据