期刊
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
卷 131, 期 3, 页码 333-338出版社
OXFORD UNIV PRESS INC
DOI: 10.1309/AJCPSVG4POONQQ1R
关键词
Cerebrospinal fluid; CSF; Body fluids; Hemacytometer; iQ200; Cell count
类别
Manual cerebrospinal fluid (CSF) and body fluid cell counts done using a hemacytometer are labor-intensive and time-consuming. The automated Iris iQ200 with the Body Fluids Module (It-is Diagnostics, Chatsworth, CA) counts RBCs and nucleated cells in CSF and other body fluids using flow cell digital imaging. We compared the cell counts from the iQ200 and the hemacytometer. CSF and body fluid samples were first analyzed in duplicate using the Neubauer hemacytometer. We then counted the samples on the iQ200. CSF samples for RBC counts of 0 to 6.7 x 10(5)/mu L and nucleated cell counts of 0 to 1,707/mu L resulted in r = 0.996 and r = 0.998, respectively. Body fluid samples for RBC counts of 0 to 2.7 x 10(5)/mu L and nucleated cell counts of 0 to 1.1 x 10(4)/mu L resulted in r = 0.988 and r = 0.987, respectively. Day-to-day of precision with quality control yielded coefficients variation of 12.5% and 13.3% for RBC counts and 9.4% and 12.3% for nucleated cell counts. Linearity studies yielded slopes of 0.99 and 1.010 for RBC and nucleated cell counts, respectively. The results from the iQ200 con-elate well with the manual hemacytometer method.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据