期刊
AMERICAN JOURNAL OF BOTANY
卷 98, 期 2, 页码 E36-E38出版社
WILEY
DOI: 10.3732/ajb.1000434
关键词
Alismataceae; cross-species transferability; genetic diversity; microsatellites; polymorphism; Sagittaria trifolia var. sinensis
资金
- National Natural Science Foundation of China (NSFC) [30771152]
Premise of the study: Microsatellite markers were developed for the aquatic plant Sagittaria trifolia var. sinensis to assess its genetic diversity and population structure. Cross-species transferability was assayed in eight congeneric species. Methods and Results: Seventeen microsatellite markers were isolated and characterized in Sagittaria trifolia var. sinensis using Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. Across the evaluated populations, 14 of the markers showed polymorphisms with 3 to 11 alleles per locus; the observed and expected heterozygosity (H-o and H-E) ranged from 0.0000 to 0.6364 and from 0.0000 to 0.8386, respectively. Nine of the loci were successfully amplified in the congeneric species. Conclusions: These markers will be useful for further investigation of population genetics in Sagittaria trifolia var. sinensis and related research in Sagittaria species.
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