期刊
AMERICAN JOURNAL OF BOTANY
卷 98, 期 2, 页码 E16-E18出版社
WILEY
DOI: 10.3732/ajb.1000410
关键词
enriched library; microsatellite; Paeonia lactiflora; primer
资金
- National Science and Technology Support Program of China [2006BAD01A1801]
- Key Project for Forestry Science Research [2006-40]
- Beijing Education Committee
Premise of the study : Microsatellite (simple sequence repeat, SSR) primers were developed for the Chinese peony, P. lactiflora, to investigate the diversity within Chinese peony germplasm resources. Methods and Results : Using an SSR-enriched genomic library, a set of 10 unique polymorphic genomic SSRs was developed and characterized. The primers amplified 61 alleles in all 10 loci, including di-, tri-, and tetranucleotide repeats. The primers were also effective for P. veitchii and P. obovata. Conclusions : The new primers will be useful for genetic research of the Chinese peony and extend the repertoire of SSR markers available to study the herbaceous taxa in Paeonia
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