期刊
AMERICAN JOURNAL OF BOTANY
卷 98, 期 4, 页码 E81-E83出版社
WILEY
DOI: 10.3732/ajb.1000356
关键词
microsatellite; polyploidy; Solanum centrale
资金
- Desert Knowledge Cooperative Research Centre
Premise of the study: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified. Methods and Results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14. Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.
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