期刊
AMERICAN JOURNAL OF BOTANY
卷 98, 期 4, 页码 E93-E95出版社
WILEY
DOI: 10.3732/ajb.1000271
关键词
ISSR-suppression PCR method; microsatellites; Myrica rubra; red bayberry
资金
- Science and Technology Project of Zhejiang Province [2008C12014-1]
- Zhejiang Natural Science Foundation [Y3080537, Y3100434]
- ZAAS
- Crop Functional Genomics Center of the 21st Century Frontier Research Program, Republic of Korea [CG3113]
Premise of the study : Microsatellite markers were developed in Myrica rubra to investigate potential hybridization events within or between M. rubra and its closely related species. Methods and Results : Using an ISSR-suppression PCR method, 12 primer pairs were temporarily developed with GSG(GT)(6) as the primer for enriching microsatellite sequences and the genomic DNA of M. rubra cv. 'Heijing' as template. The average allele number per locus was 4.9 within 26 individuals including two species, M. rubra and M. nana. Three pairs of primers produced species-specific alleles, and the other nine showed polymorphisms among 26 accessions. Conclusions : Results indicated that the ISSR-suppression PCR method is suitable for developing microsatellite markers, especially for this species with little understanding of genomic information. The developed microsatellite markers provide a useful tool for further studies of population structure within or between M. rubra and M. nana or other closely related species.
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