期刊
AMERICAN JOURNAL OF BOTANY
卷 98, 期 3, 页码 E61-E63出版社
WILEY
DOI: 10.3732/ajb.1000456
关键词
genetic diversity; Gynostemma pentaphyllum; microsatellites.
资金
- National Basic Research Program of China (973 program) [2007CB411600]
Premise of the study: Microsatellite primers were developed for Gynostemma pentaphyllum, a traditional Chinese medicinal herb, to investigate its population genetic diversity. Methods and Results: Using the Microsatellite Sequence Enrichment protocol, 14 polymorphic primers sets were identified in four Chinese Gynostemma pentaphyllum populations. The primers amplified di- and trinucleotide repeats with 1-6 alleles per locus, and the observed heterozygosities ranged from 0.000 to 1.000 per population. Conclusions: These markers will facilitate further studies on genetic structure and gene flow of Gynostemma pentaphyllum populations.
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