期刊
AMERICAN JOURNAL OF BOTANY
卷 97, 期 12, 页码 E159-E161出版社
WILEY
DOI: 10.3732/ajb.1000377
关键词
Cycas panzhihuaensis; expressed sequence tags; microsatellite
资金
- National Basic Research Program of China (973 Program) [2007CB411601]
- West Light Foundation of the Chinese Academy of Sciences
- Natural Science Foundation of Yunnan Province [2007C089M, 2007C087M]
Premise of the study: Cycas panzhihuaensis L. Zhou & S. Y. Yang is a vulnerable gymnosperm endemic to China, where its range represents the northernmost occupation of Cycas L. We developed EST-derived SSR markers to investigate its genetic diversity and population structure. Methods and Results: Based on the expressed sequence tag (EST) database for Cycas rumphii Miq., seven simple sequence repeat (SSR) markers were identified and screened in 55 individuals from seven wild populations of C. panzhihuaensis. Alleles numbered 2 to 5, and their observed heterozygosity and expected heterozygosity ranged from 0.0000 to 0.6545 and from 0.0535 to 0.6966, respectively. Conclusions: These new EST-SSR markers will enhance further studies of the population genetics of C. panzhihuaensis, allowing researchers to design reasonable conservation and management protocols.
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