Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Medicine, General & Internal
Jeong-Hwa Kim, Jae-Woon Oh, Young Lee, Jeong-Ho Yun, Seong-Ho Choi, Dong-Woon Lee
Summary: This study found that detecting 18 bacterial species in mouth-rinse samples can be used to diagnose periodontitis, with Treponema denticola, Prevotella nigrescens, and Streptococcus mitis significantly associated with severe periodontitis. Further research is needed to assess the potential of oral-rinsing solutions in indicating oral infection risk and improving oral hygiene.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Immunology
Wei-Hsuan Lin, Han-Cheng Chiu, Kuan-Fu Chen, Kuo-Chien Tsao, Yi-Yin Chen, Ting-Hsuan Li, Yhu-Chering Huang, Yu-Chia Hsieh
Summary: In adult patients with CAP, bacterial pathogens accounted for 50% and viruses for 36.3%. Molecular testing could detect more pathogens than traditional culture techniques in diagnosing pneumonia. Co-infections with multiple pathogens, especially K. pneumoniae and human rhinovirus, were common in this study.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Nanoscience & Nanotechnology
Kyung Hoon Kim, MinHo Yang, Younseong Song, Chi Hyun Kim, Young Mee Jung, Nam-Ho Bae, Sung-Jin Chang, Seok Jae Lee, Yong Tae Kim, Bong Gill Choi, Kyoung G. Lee
Summary: The development of a three-dimensional nanoweb film has enabled efficient capture of bacterial pathogens through hand-touching, demonstrating high capture efficiency and sensitivity. This unique nanostructure holds great potential for applications in capturing and detecting bacteria.
Article
Chemistry, Multidisciplinary
Maigualida Cuenca, Maria Jose Marin, Lourdes Novoa, Ana O'Connor, Maria Carmen Sanchez, Juan Blanco, Jacobo Limeres, Mariano Sanz, Pedro Diz, David Herrera
Summary: The study aimed to investigate the subgingival microbiota in patients with Down syndrome, finding that those with periodontitis had higher levels of anaerobic bacteria, T. forsythia, and E. corrodens. Additionally, periodontitis patients were older, had lower rates of hypothyroidism, and higher levels of dental plaque.
APPLIED SCIENCES-BASEL
(2021)
Article
Food Science & Technology
Mengtao Chen, Xinyue Lan, Longjiao Zhu, Ping Ru, Wentao Xu, Haiyan Liu
Summary: In order to ensure food safety, it is necessary to detect viable bacteria in a short period of time. Traditional bacterial culture methods are time-consuming, so PCR mediated nucleic acid molecular recognition technologies have been developed to quickly and accurately detect foodborne pathogens.
Article
Parasitology
Gereon Schares, Majda Globokar Vrhovec, Mareen Tuschy, Maike Joeres, Andrea Baerwald, Bretislav Koudela, Jitender P. Dubey, Pavlo Maksimov, Franz J. Conraths
Summary: The 529-bp repeat of H. hammondi can be used to establish a quantitative real-time PCR assay, with excellent specificity and sensitivity. This assay is a valuable diagnostic tool for epidemiological and cell biological studies on H. hammondi and related parasites.
PARASITES & VECTORS
(2021)
Review
Oncology
Jordana Williams, Morris Kostiuk, Vincent L. Biron
Summary: HPV is responsible for most cervical cancers and some head and neck cancers. Specific detection methods are used to diagnose HPV infections in cervical and head and neck cancers.
FRONTIERS IN ONCOLOGY
(2022)
Article
Medicine, Research & Experimental
Cheng-Yih Su, Hideo Shigeishi, Hiroshi Murodumi, Masaru Sugiyama, Kouji Ohta, Toshinobu Takemoto
Summary: This study clarified the association between oral Epstein-Barr virus (EBV) and periodontitis, showing a significant relationship in middle-aged and older Japanese individuals. Particularly, a higher EBV DNA positivity rate was found in patients with poor periodontal health, indicating a potential link between oral EBV and periodontal disease.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Optics
Damian Presti, Julian Bergier, Lucas Ripoll, Cristina Borio, Gustavo A. Torchia, Marcos Bilen
Summary: Since the global pandemic of SARS-CoV-2, the need for a low-cost and faster diagnostic tool for evaluating symptomatic patients has emerged. Point-of-care testing and point-of-need testing systems have been developed to provide accurate and rapid diagnostics. In this study, a bio-photonic device using an iso-thermal system was developed for detecting SARS-CoV-2. The device showed comparable sensitivity to the commercially used reference method and was built with simple and low-cost components, making it a high-efficiency and affordable instrument.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2023)
Article
Veterinary Sciences
Zoran Zlabravec, Brigita Slavec, Al Vrezec, Urska Kuhar, Olga Zorman Rojs, Zlatko Golob, Jozko Racnik
Summary: This study detected the presence of herpesviruses in wild birds and investigated their phylogenetic relationship using PCR. The results showed that herpesviruses are present in a wide range of bird species and can be transmitted through predation and superpredation. Simultaneous testing of oropharyngeal and cloacal swabs is necessary for accurate herpesvirus detection in wild birds.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Analytical
Huizhen Li, Yunhui Li, Cenlin Gui, Daqi Chen, Lanfang Chen, Le Luo, Guobao Huang, Yang Yuan, Rong He, Fan Xia, Jiahai Wang
Summary: In this study, it has been confirmed that bare glass nanopores can accurately measure various lengths of DNA duplexes without adding organic solvent and embedding polymer hydrogel. The measurement has a resolution of 200 base pairs and shows well-separated peak amplitudes of blockage currents. By utilizing this capability, amplicons from PCR amplification can be discriminated without fluorescent labeling. This method can also discriminate various pathogenic bacteria and viruses, including SARS-CoV-2 and its mutants, in clinical samples with high resolution. Furthermore, the bare glass nanopore can quantitatively measure PCR amplicons without the need for extra labeling, with a detection range from 0.75 aM to 7.5 pM and a detection limit of 7.5 aM.
Article
Pediatrics
Hyun-Woo Lee, Seung-Beom Han, Jung-Woo Rhim
Summary: This study aimed to determine the subjects for bacterial multiplex polymerase chain reaction (mPCR) testing and to interpret the mPCR test results based on patients' clinical symptoms and diagnoses. The study found that positive mPCR test results were significantly associated with older age, diagnosis of acute gastroenteritis, presence of hematochezia, and absence of cough. Additionally, the identification of invasive pathogens in patients with positive mPCR test results was significantly associated with the diagnosis of acute gastroenteritis, presence of fever and diarrhea, and higher C-reactive protein levels. Therefore, selective bacterial mPCR testing should be performed based on the patients' clinical symptoms and diagnoses.
Article
Microbiology
Maria Daniela Silva, Antonio Lima, Nuno Marcal, Luis Dias, Miguel Gama, Sanna Sillankorva
Summary: Understanding the bacterial causes of otitis media is crucial for effective treatment. This study analyzed samples from 49 children with otitis media in Portugal, finding a correlation between bacteria in the middle ear fluid and nasopharynx. The most commonly identified species were Moraxella catarrhalis and Streptococcus pneumoniae, but Haemophilus influenzae was more prevalent in the middle ear fluid. The study also revealed the importance of polymicrobial infections in otitis media.
Article
Biochemistry & Molecular Biology
Kazuki Izawa, Kazuko Okamoto-Shibayama, Daichi Kita, Sachiyo Tomita, Atsushi Saito, Takashi Ishida, Masahito Ohue, Yutaka Akiyama, Kazuyuki Ishihara
Summary: The study conducted a metagenomic analysis of subgingival plaque samples from Japanese individuals with and without periodontitis, revealing significant differences in the composition of Bacteroides and Mycobacterium genera between healthy and diseased sites, as well as in the relative abundance of functional gene categories such as carbohydrate metabolism and amino acid metabolism. These results provide important insights into the dysbiosis-induced changes in taxonomic and functional gene category abundance during periodontal disease progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Saki Nakane, Kentaro Imamura, Rio Hisanaga, Kazuyuki Ishihara, Atsushi Saito
Summary: The study demonstrated that CTLA-4-Ig can effectively inhibit bone resorption in periodontitis by affecting osteoclast differentiation and activity, potentially through the regulation of the NF-kappa B pathway and PP2A expression.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Masahiro Shimogishi, Takayasu Watanabe, Masaki Shibasaki, Takahiko Shiba, Keiji Komatsu, Takashi Nemoto, Kazuyuki Ishihara, Yoshio Nakano, Takanori Iwata, Shohei Kasugai, Ichiro Nakagawa
Summary: The study found that the bacterial composition in the peri-implant sulcus was similar among patients at 1 and 2 weeks post-uncovering, with predominance of Firmicutes and Proteobacteria. However, the composition became more diverse and significantly different at 4 and 6 weeks. This suggests that the peri-implant microbiota is established early after implant uncovering, but changes over time and varies between patients.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Immunology
Eitoyo Kokubu, Yuichiro Kikuchi, Kazuko Okamoto-Shibayama, Shuichi Nakamura, Kazuyuki Ishihara
Summary: The study demonstrates that dentilisin plays a role in the crawling-dependent surface spreading of T. denticola by affecting its motility speed and efficiency, as evidenced by the smaller colony area, lower crawling velocity, and reduced crawling efficiency observed in dentilisin-deficient strains compared to wild-type and Msp mutant strains.
MICROBIOLOGY AND IMMUNOLOGY
(2021)
Article
Medicine, General & Internal
Shuichi Nishikubo, Hiroyuki Matsuda, Shinya Watanabe, Hidetoshi Tamura, Morio Tonogi
Summary: The novelty of this procedure lies in the reconstruction of a maxillary palatal defect using two pedicled flaps: a pedicled buccal fat pad and a nasolabial flap. This combined flap approach simplifies the surgery, reduces its duration, and extends its applicability to a broader range of defects.
CLINICAL CASE REPORTS
(2021)
Article
Microbiology
Yuichiro Kikuchi, Kazuko Okamoto-Shibayama, Eitoyo Kokubu, Kazuyuki Ishihara
Summary: Among the bacteria inhabiting the human oral cavity, Capnocytophaga ochracea plays a significant role in the early phase of dental plaque formation on tooth surfaces. Its response to oxidative stress may be linked to the hydrogen peroxide-sensing transcriptional regulator OxyR.
Article
Dentistry, Oral Surgery & Medicine
Eitoyo Kokubu, Yuichiro Kikuchi, Kazuko Okamoto-Shibayama, Kazuyuki Ishihara
Summary: This study investigated the effects of T. denticola virulence factors dentilisin and Msp on epithelial cells. The results showed that T. denticola disrupts the function of epithelial cells by hydrolyzing intercellular junction proteins and focal adhesion proteins, inhibiting cell migration and healing.
BULLETIN OF TOKYO DENTAL COLLEGE
(2022)
Article
Clinical Neurology
Shouhei Ogisawa, Shuichi Nishikubo, Junya Nakajima, Hiroaki Azaki, Kotoe Mayahara, Keiji Shinozuka, Morio Tonogi
Summary: The study investigates the timing of hyoid bone relocation after maxillomandibular advancement (MMA) and genioglossus advancement (GA) surgeries for patients with obstructive sleep apnea (OSA). The findings suggest that the movement of the hyoid bone towards the oral side after one year post-surgery is correlated with the improvement of the apnea-hypopnea index (AHI).
SLEEP AND BREATHING
(2023)
Article
Dentistry, Oral Surgery & Medicine
Yuri Miyai-Murai, Kazuko Okamoto-Shibayama, Toru Sato, Yuichiro Kikuchi, Eitoyo Kokubu, Jan Potempa, Kazuyuki Ishihara
Summary: The Msp protein complex and the serine protease dentilisin are the best-characterized virulence factors in Treponema denticola, the major etiological agent of chronic periodontitis. In addition, the cysteine protease dentipain also contributes to pathogenicity, but its secretion, processing, cellular localization, and role in T. denticola virulence are not fully understood. This study found that dentipain is located in the outer sheath derived from T. denticola dentilisin- and Msp-deficient mutants, suggesting that dentilisin and Msp are involved in the secretion and maturation of dentipain. Inactivation of the dentipain gene slowed the growth of T. denticola, and the mutant strain showed altered gene expression, increased hydrophobicity, reduced autoaggregation activity, and decreased adhesion to IgG.
MOLECULAR ORAL MICROBIOLOGY
(2023)
Article
Microbiology
Yukiko Yamaguchi-Kuroda, Yuichiro Kikuchi, Eitoyo Kokubu, Kazuyuki Ishihara
Summary: This study investigated the effect of diffusible signaling molecules from P. gingivalis on F. nucleatum and revealed that these molecules induce metabolic changes in F. nucleatum, including an increase in polysaccharide synthesis and a reduction in de novo synthesis of purine and pyrimidine. These changes may accelerate biofilm formation by F. nucleatum with P. gingivalis and could serve as potential therapeutic targets for preventing dysbiosis.
JOURNAL OF ORAL MICROBIOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Shigeru Ishii, Kohsuke Ohno, Hiroto Saito, Atsushi Nakamura, Shuichi Nishikubo
BRITISH JOURNAL OF ORAL & MAXILLOFACIAL SURGERY
(2023)
Article
Medicine, General & Internal
Takahiko Shiba, Takayasu Watanabe, Keiji Komatsu, Tatsuro Koyanagi, Takashi Nemoto, Yujin Ohsugi, Yasuyuki Michi, Sayaka Katagiri, Yasuo Takeuchi, Kazuyuki Ishihara, Takanori Iwata
Summary: A successful case of non-surgical treatment for peri-implantitis with horizontal bone resorption in a 68-year-old male patient was reported, showing decreased probing depth and recovery of bacterial composition at both sites. The clinical condition remained stable for 7 years after treatment.
SAGE OPEN MEDICAL CASE REPORTS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Shuichi Nishikubo, Shouhei Ogisawa, Junya Nakajima, Hiroaki Azaki, Keiji Shinozuka, Morio Tonogi
Summary: Backsliding is a major concern when moving the maxilla significantly forward in orthognathic surgery, as it can have negative impacts on widening the pharyngeal airway postoperatively. Therefore, a surgical method has been devised to prevent maxillary retrusion by adjusting the bone during maxillary advancement.
JOURNAL OF ORAL SCIENCE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Kazuko Okamoto-Shibayama, Takenobu Warita, Eitoyo Kokubu, Daichi Kita, Yuichiro Kikuchi, Kazuyuki Ishihara
Summary: The study found that the hyalin-like protein in Capnocytophaga ochracea is involved in gliding activity, but its relationship with biofilm formation is not significant.
BULLETIN OF TOKYO DENTAL COLLEGE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Kazuko Okamoto-Shibayama, Ayumi Yoshida, Kazuyuki Ishihara
Summary: This study demonstrated that resveratrol has inhibitory effects on the formation of Candida albicans biofilms at developmental, sustained, and mature stages. The antifungal activity of resveratrol shows potential as a treatment and prevention tool against Candida albicans by inhibiting the growth of existing or forming biofilms.
BULLETIN OF TOKYO DENTAL COLLEGE
(2021)
Article
Cardiac & Cardiovascular Systems
Xiaoxi Yao, Zachi I. Attia, Emma M. Behnken, Melissa S. Hart, Shealeigh A. Inselman, Kayla C. Weber, Fan Li, Nikki H. Stricker, John L. Stricker, Paul A. Friedman, Peter A. Noseworthy
Summary: This study aims to explore whether Apple Watch, used as a long-term monitoring device, is effective in the early diagnosis of atrial fibrillation (AF) and the prevention of cognitive function decline in older adults. By using AI-ECG to screen high-risk patients and record electrocardiograms, the effectiveness of Apple Watch will be evaluated. If successful, this approach could have significant implications on how future clinical practice leverages consumer devices for early diagnosis and disease prevention.
AMERICAN HEART JOURNAL
(2024)