Article
Immunology
A. Martin-Montes, M. Recuero, I Sastre, E. Vilella, M. Rosich-Estrago, M. Atienza, J. L. Cantero, A. Frank-Garcia, M. J. Bullido
Summary: This study compared cholesterol levels and immune cell changes in cognitively healthy individuals, mild cognitive impairment (MCI) patients, and Alzheimer's Disease (AD) patients. The results showed lower levels of free cholesterol in peripheral blood mononuclear cells (PBMC) of MCI and AD patients. Additionally, a decrease in CD8+ cells and an increase in CD16+ cells were found in AD patients. These findings suggest that cholesterol levels in PBMCs may serve as an early signature of AD and highlight the involvement of the immune system in the disease.
JOURNAL OF NEUROIMMUNOLOGY
(2022)
Article
Psychiatry
Adam Wysokinski, Elzbieta Kozlowska, Ewa Szczepocka, Anna Lucka, Justyna Agier, Ewa Brzezinska-Blaszczyk, Katarzyna Sobierajska
Summary: Schizophrenia patients exhibit a distinctive mRNA expression profile of dopamine and serotonin receptors in peripheral blood mononuclear cells. This expression is minimally affected by antipsychotic treatment, suggesting it may serve as a useful diagnostic biomarker for schizophrenia.
FRONTIERS IN PSYCHIATRY
(2021)
Article
Pharmacology & Pharmacy
Vera Vorstandlechner, Dragan Copic, Katharina Klas, Martin Direder, Bahar Golabi, Christine Radtke, Hendrik J. Ankersmit, Michael Mildner
Summary: This study demonstrates the anti-fibrotic effect of PBMCsec on cutaneous scars in both mouse and human experimental settings. PBMCsec regulates the expression of fibrosis-related genes, attenuates myofibroblast differentiation, and inhibits the breakdown of elastic fibers. These findings suggest that PBMCsec could be a novel therapeutic option for treating skin scarring.
Article
Materials Science, Multidisciplinary
Byeongyeon Kim, In-Ho Seo, Gwang Myeong Seo, Woo-Joong Kim, Eui-Cheol Shin, Sungyoung Choi
Summary: The study introduces a blood divider (B-DV) as a centrifuge tube insert to prevent RBC contamination during PBMC harvest, simplifying the separation process and enabling rapid, easy PBMC isolation.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Biochemistry & Molecular Biology
Belay Tessema, Janine Riemer, Ulrich Sack, Brigitte Koenig
Summary: The results of CSA parameters measured in PBMCs are influenced by the PBMC isolation method, age, seasonal variation, and gender. Researchers and physicians should properly interpret the results of CSA parameters in PBMCs by considering these factors. Separate CSA evaluation standards should be used based on the isolation method, age, gender, and season-dependent factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Suji Kim, Hyun-Eui Park, Woo Bin Park, Seo Yihl Kim, Hong-Tae Park, Han Sang Yoo
Summary: The study investigated the host immune response against Mycobacterium avium in dogs, revealing that different immune responses are triggered at different time points after infection. Early induction of Th1-associated genes was observed, followed by an increase in Th17-associated genes. Additionally, a decrease in apoptosis-related gene expression and an increase in intracellular M. avium abundance were observed in monocyte-derived macrophages after 24 hours of infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Oncology
Sheng-Cheng Wu, Shiue-Wei Lai, Xin-Jie Lu, Hsing-Fan Lai, Yu-Guang Chen, Po-Huang Chen, Ching-Liang Ho, Yi-Ying Wu, Yi-Lin Chiu
Summary: This study identified differentially expressed miRNAs in CML patients and comprehensively analyzed their biological functions in association with target genes. The results showed that upregulated miRNAs are associated with apoptosis and downregulated miRNAs are associated with cell cycle. The defined DE-miRNAs were also found to be associated with the prognosis of CML patients.
FRONTIERS IN ONCOLOGY
(2023)
Article
Medicine, General & Internal
Keiichiro Yoshioka, Hironori Sato, Takeshi Kawasaki, Daisuke Ishii, Takuro Imamoto, Mitsuhiro Abe, Yoshinori Hasegawa, Osamu Ohara, Koichiro Tatsumi, Takuji Suzuki
Summary: RNA sequencing analysis revealed differential gene expression patterns in peripheral blood mononuclear cells (PBMCs) between patients with pulmonary sarcoidosis and healthy controls, with 270 differentially expressed genes involved in various biological processes related to the pathobiology of sarcoidosis. Enrichment analysis of gene ontology and KEGG pathways indicated the potential involvement of specific signaling pathways in sarcoidosis pathogenesis, providing insight into potential therapeutic targets for the disease.
FRONTIERS IN MEDICINE
(2022)
Article
Oncology
Jacobo Rogado, Fernando Pozo, Kevin Troule, Jose Miguel Sanchez-Torres, Nuria Romero-Laorden, Rebeca Mondejar, Olga Donnay, Anabel Ballesteros, Vilma Pacheco-Barcia, Javier Aspa, Fatima Al-Shahrour, Arantzazu Alfranca, Ramon Colomer
Summary: Peripheral blood mononuclear cells (PBMCs) have been explored as potential biomarkers to predict the efficacy of anti-PD-1 immunotherapy in advanced non-small cell lung cancer (NSCLC) patients. High baseline levels of circulating T cell subpopulations related to tissue lymphocyte recruitment were found to be associated with poorer outcomes in immunotherapy-treated advanced NSCLC patients.
Article
Cell Biology
Abhirup Bagchi, Aneesha Nath, Vasanth Thamodaran, Smitha Ijee, Dhavapriya Palani, Vignesh Rajendiran, Vigneshwaran Venkatesan, Phaneendra Datari, Aswin Anand Pai, Nancy Beryl Janet, Poonkuzhali Balasubramanian, Yukio Nakamura, Alok Srivastava, Kumarasamypet Murugesan Mohankumar, Saravanabhavan Thangavel, Shaji R. Velayudhan
Summary: This study established a protocol for culturing early-stage EPCs from peripheral blood and immortalizing them by expressing HEE genes, providing a new cellular model for studying the molecular mechanisms of human erythropoiesis. The two iEPCs generated showed stable doubling times and erythroid progenitor properties, suitable for studying therapeutic targets and mechanisms of red cell diseases.
Article
Cell & Tissue Engineering
Chang-Han Chuang, Chi-Chung Kuo, Yueh-Feng Chiang, Pei-Yuan Lee, Fu-Hui Wang, Chia-Ying Hsieh, Ching- Shen, Yu-Hsuan Chung, Kuan-Der Lee, Shih-Fang Wu, Hong-Lin Su, Chih-Lung Lin
Summary: This study examines the effect of peripheral blood-derived mononuclear cells (PBMNCs) on patients with knee osteoarthritis (KOA). The study found that intra-articular injection of autologous PBMNCs significantly attenuated knee pain and motor dysfunction in patients, and the therapeutic effect could be stably maintained for up to 24 months.
CELL TRANSPLANTATION
(2023)
Article
Environmental Sciences
Milda Babonaite, Matas Cepulis, Jurate Kazlauskaite, Juozas Rimantas Lazutka
Summary: According to PlasticEurope, global plastics production reached 390.7 million tons in 2021. Unfortunately, a large portion of these plastics end up as waste in the ocean or on land due to their non-biodegradable nature. This study focused on evaluating the internalization rates, cytotoxicity, and genotoxicity of polystyrene nanoparticles (PS-NPs) in human peripheral blood mononuclear cells (PBMCs) in vitro. The results showed that while PS-NPs did not have a cytotoxic effect on PBMCs, they did induce primary DNA damage and micronucleated cells, indicating their genotoxic potential.
Review
Medicine, General & Internal
Francois Sauer, Marianne Riou, Anne-Laure Charles, Alain Meyer, Emmanuel Andres, Bernard Geny, Samy Talha
Summary: Heart failure is a leading cause of hospitalization in patients aged over 65 and is associated with high mortality rates. Recent studies have focused on the mitochondrial respiration of peripheral blood circulating cells (PBMCs) in order to better understand the physiopathology of HF. These studies suggest that cardiovascular metabolic risk factors and cellular switch may contribute to decreased PBMC mitochondrial respiration, which is associated with HF severity. Further research is needed to investigate the potential diagnostic, prognostic, and therapeutic utility of monitoring PBMC mitochondrial function and mitoDAMPs in HF patients.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Hector Godoy-Marin, Romain Duroux, Kenneth A. Jacobson, Concepcio Soler, Hildegard Colino-Lage, Veronica Jimenez-Sabado, Jose Montiel, Leif Hove-Madsen, Francisco Ciruela
Summary: Atrial fibrillation (AF) is influenced by various factors such as oxidative stress, calcium overload, inflammation, as well as adenosine and its receptors. Studies have found increased A2AR expression in the right atrium of AF patients, elevated levels of adenosine content in plasma, and reduced ADA activity. Importantly, there is a positive correlation between A2AR expression in PBMCs and in the right atrium.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Clinical Neurology
Stylianos Ravanidis, Anastasia Bougea, Dimitra Karampatsi, Nikolaos Papagiannakis, Matina Maniati, Leonidas Stefanis, Epaminondas Doxakis
Summary: This study identified specific circRNAs in blood mononuclear cells of patients with Parkinson's disease, which may serve as diagnostic biomarkers for PD. The circRNAs are derived from genes with essential brain functions and may hint on the PD pathways affected.
MOVEMENT DISORDERS
(2021)
Review
Psychiatry
Marco Ferrari, Marco Godio, Stefano Martini, Camilla Callegari, Marco Cosentino, Franca Marino
Summary: The neurobiology of psychiatric disorders is increasingly understood, and immuno-inflammatory mechanisms are believed to play a central role. The antipsychotic drug quetiapine has been shown to modulate dopamine and serotonin signaling, which in turn can affect immune functions. However, studies on the immune effects of quetiapine have not provided conclusive results, highlighting the need for further research in this field.
INTERNATIONAL JOURNAL OF PSYCHIATRY IN CLINICAL PRACTICE
(2023)
Article
Clinical Neurology
Marco Ferrari, Marco Godio, Stefano Martini, Camilla Callegari, Marco Cosentino, Franca Marino
Summary: This study investigated the correlation between levels of inflammatory markers and the effects of quetiapine (QUE) in bipolar disorder (BD) patients. The results showed that QUE treatment significantly reduced CRP and IL-6 plasma levels. Furthermore, higher CRP and IL-6 plasma levels at baseline were correlated with better improvement of patients' clinical symptoms.
HUMAN PSYCHOPHARMACOLOGY-CLINICAL AND EXPERIMENTAL
(2022)
Article
Biochemistry & Molecular Biology
Marco Cosentino, Franca Marino
Summary: COVID-19 mRNA vaccines, unlike traditional vaccines, resemble pharmaceutical drugs as they contain active SARS-CoV-2 S protein mRNA that leads to the production of active protein. Accurately defining COVID-19 mRNA vaccines as pharmaceutical drugs is crucial for their evaluation and safe use.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Psychiatry
Serena Borroni, Linda Franchini, Silvia Brioschi, Giulia Vassena, Elisabetta Masci, Chiara Franzoni, Giulia Ruotolo, Cristina Colombo, Andrea Fossati
Summary: This study examines the role of sociodemographic and clinical variables as well as personality pathological features in predicting high lethality suicide attempts. The results indicate that previous suicide attempts and current suicide ideation play a role in predicting serious suicide attempts. Specifically, the Detachment domain is associated with high lethality suicide attempts and remains a significant predictor even when considering the effects of previous attempts and ideation.
PERSONALITY AND MENTAL HEALTH
(2023)
Article
Clinical Neurology
Elena Mazza, Federico Calesella, Marco Paolini, Camilla di Pasquasio, Sara Poletti, Cristina Lorenzi, Andrea Falini, Raffaella Zanardi, Cristina Colombo, Francesco Benedetti
Summary: Our study investigated the effects of insulin and its derived measures on white matter microstructure and neural functional connectivity in patients with bipolar disorder (BD). The results showed that insulin was negatively associated with fractional anisotropy and positively influenced radial diffusivity and mean diffusivity. Additionally, insulin resistance had a significant effect on radial diffusivity, while insulin sensitivity was positively associated with fractional anisotropy and negatively associated with radial diffusivity and mean diffusivity. These findings suggest that insulin may play a role in the pathophysiology of BD by affecting white matter microstructure and functional connectivity.
Review
Biochemistry & Molecular Biology
Stefano Martini, Alessandra Gemma, Marco Ferrari, Marco Cosentino, Franca Marino
Summary: Cannabidiol (CBD), the main non-psychotropic cannabinoid derived from cannabis, has been approved for the treatment of seizures associated with certain syndromes and has shown potential in treating inflammation and immune-related conditions. Preclinical studies have demonstrated CBD's inhibitory effects on cytokine production and tissue infiltration, suggesting its therapeutic role in diseases with inflammatory components. However, clinical studies are needed to validate its efficacy in conditions such as multiple sclerosis, autoimmune diseases, cancer, asthma, and cardiovascular diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Obstetrics & Gynecology
Marco Cosentino, Franca Marino
JOURNAL OF PERINATOLOGY
(2023)
Article
Clinical Neurology
Benedetta Vai, Federico Calesella, Claudia Lenti, Lidia Fortaner-Uya, Elisa Caselani, Paola Fiore, Sigrid Breit, Sara Poletti, Cristina Colombo, Raffaella Zanardi, Francesco Benedetti
Summary: Suicide attempts in Bipolar Disorder are characterized by high levels of lethality and impulsivity. Reduced rates of amygdala and cortico-limbic habituation can identify a fMRI phenotype of suicidality in the disorder related to internal over-arousing states. This study found that bipolar suicide attempters have lower habituation levels in several cortico-limbic areas, suggesting that reduced habituation in the cortico-limbic system may serve as a biomarker for suicidality. Machine learning techniques achieved high accuracy in differentiating suicide attempters from non-attempters.
PSYCHIATRY RESEARCH-NEUROIMAGING
(2023)
Letter
Cardiac & Cardiovascular Systems
Marco Cosentino, Franca Marino
Article
Biochemistry & Molecular Biology
Alessia Furgiuele, Franca Marino, Emanuela Rasini, Massimiliano Legnaro, Alessandra Luini, Maria Giulia Albizzati, Alessia di Flora, Barbara Pacchetti, Marco Cosentino
Summary: This study investigated the effects of CBD on human peripheral blood mononuclear cells (PBMCs) and CD4+ T cells, and found that CBD can affect cytokine production, cell proliferation, and the functional properties of regulatory T cells. However, the effects of CBD on cell proliferation and protein production were inconsistent, suggesting a complex activity that warrants further investigation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Marco Paolini, Yasmin Harrington, Federica Colombo, Valentina Bettonagli, Sara Poletti, Matteo Carminati, Cristina Colombo, Francesco Benedetti, Raffaella Zanardi
Summary: A study found that there is a correlation between MRI imaging and treatment response in patients with major depressive disorder (MDD). The study analyzed MRI scans of 100 patients and found that lower grey matter volumes, particularly in the hippocampus and temporal lobe-related areas, were associated with worse treatment outcomes. Functional connectivity patterns were also found to be related to treatment response.
JOURNAL OF PSYCHOPHARMACOLOGY
(2023)
Letter
Criminology & Penology
Lorenzo Fregna, Francesco Attanasio, Guido Travaini, Cristina Colombo
VIOLENCE AND GENDER
(2023)
Review
Immunology
Alessia Furgiuele, Frederico C. Pereira, Stefano Martini, Franca Marino, Marco Cosentino
Summary: Parkinson's disease is a neurodegenerative disease with no available treatment options that can effectively delay its progression. The immune system has been identified as playing a critical role in this disease, suggesting that immune-based approaches may hold potential as antiparkinsonian agents. Dopaminergic pathways are known to regulate immune responses, but the full extent of the immune regulatory effects of dopaminergic agents is not yet fully understood.
CLINICAL & TRANSLATIONAL IMMUNOLOGY
(2023)
