Article
Agricultural Engineering
Malihe Pooresmaeil, Hassan Namazi
Summary: Recently, covalent organic frameworks (COFs) have been recognized as efficient drug-loading platforms due to their desired structure. In this study, a spherical COF was constructed as a platform for loading 5-fluorouracil (5-Fu). The synthesized COF exhibited a larger pore size than the dimensions of 5-Fu and had a high surface area. However, the imine bond in COFs was found to be relatively unstable in acidic conditions. To address this issue, COF/5-Fu was capped into a carboxymethyl starch-gelatin (CMS-Gel) coat, resulting in COF/5-Fu@CMS-Gel. Drug release tests showed that the loaded 5-Fu was released at a higher rate in pH 7.4 compared to pH 1.2. The structural analyses confirmed the successful formation of the prepared samples, and MTT assays demonstrated the biocompatible nature and cytotoxic potential of neat COF@CMS-Gel after drug loading. These findings suggest that COF/5-Fu@CMS-Gel has promising applications in controlled colon drug delivery.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Toxicology
Daniela Rodrigues, Roel van Kampen, Ad A. van Bodegraven, Jos C. S. Kleinjans, Danyel G. J. Jennen, Theo M. de Kok
Summary: This study aims to explore the molecular mechanisms of 5-FU-induced colon toxicity by comparing gene expression responses in colon tissue and an in vitro model of breast cancer patients receiving capecitabine treatment. The study identified 31 affected genes related to toxicity, involving pathways related to molecular transport, cellular responses, metabolism, and immune system. The gene expression of TRIM31 was similarly altered in the in vitro model, suggesting its potential as a biomarker for drug safety screening in the future.
TOXICOLOGY LETTERS
(2022)
Article
Anatomy & Morphology
Xueni Wei, Zhonghui Yang, Guomei Chen, Ji Huang
Summary: High expression of VMP1 in colon cancer cells can promote exosome secretion, which leads to the acquisition of more drug-resistant properties by cancer cells.
Article
Cell Biology
Huan Niu, Meng Zhao, Jing Huang, Jing Wang, Yang Si, Shan Cheng, Wei Ding
Summary: CBs may serve as indicators of drug sensitivity or resistance in tumor cells, while UHMK1 maintains the structure and morphology of CBs by regulating splicing events.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Pathology
Guangyi Liu, Huan Wang, Rui Ran, Yicheng Wang, Yang Li
Summary: The study investigated the molecular mechanism of FOSL1 regulating 5-FU resistance in colon cancer. It was found that high expression of FOSL1 was associated with 5-FU resistance and poor prognosis in colon cancer. Low expression of FOSL1 enhanced 5-FU sensitivity, suppressed cell proliferation, and induced apoptosis in colon cancer cells. Mechanistically, FOSL1 activated PHLDA2 and promoted glycolysis, leading to increased 5-Fu resistance and cell proliferation.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Oncology
Yu Zheng, Jiansheng Wu, Hong Chen, Dajia Lin, Hongyuan Chen, Junyin Zheng, Haoyun Xia, Liangxiang Huang, Changqing Zeng
Summary: In this study, it was found that KLF4 enhances the sensitivity of colon cancer cells to 5-FU by targeting RAB26 to suppress the autophagy pathway.
CANCER BIOLOGY & THERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Fangyuan Cao, Mumu Shi, Bo Yu, Xiangrong Cheng, Xin Li, Xinshan Pa
Summary: The study demonstrated that both 5-fluorouracil (5-Fu) and cisplatin (CDDP) induce apoptosis in A549 and NCI-H226 cells, while also increasing the expression of certain miRNAs. Interestingly, 5-Fu was found to enhance OCT3/4 expression in A549 cells compared to CDDP treatment, suggesting genetic differences between the two treatments in methylation patterns. Additionally, changes in the expression of methyltransferase genes and DNA methylation were noted in A549 cells treated with 5-Fu.
ONCOTARGETS AND THERAPY
(2021)
Article
Oncology
Chalirmporn Atasilp, Rinradee Lenavat, Natchaya Vanwong, Phichai Chansriwong, Ekaphop Sirachainan, Thanyanan Reungwetwattana, Pimonpan Jinda, Somthawin Aiempradit, Suwannee Sirilerttrakul, Monpat Chamnanphon, Apichaya Puangpetch, Nipaporn Sankuntaw, Patompong Satapornpong, Chonlaphat Sukasem
Summary: This study investigated the effects of MTHFR polymorphisms on hematological toxicity and treatment efficacy of 5-FU therapy in colorectal cancer patients. The results showed that the 677G>A polymorphism was significantly associated with leukopenia, neutropenia, and thrombocytopenia, while the 1298A>C polymorphism had no significant association with hematological toxicity.
FRONTIERS IN ONCOLOGY
(2022)
Article
Pharmacology & Pharmacy
Safiye Akkin, Gamze Varan, Davut Aksut, Milo Malanga, Ayse Ercan, Murat Sen, Erem Bilensoy
Summary: This study developed a CD nanoplex system based on charge interaction for delivering 5-FU and IL-2, which showed efficient drug release, stability, and high anticancer effect against colorectal cancer cells. Additionally, it demonstrated potential as an oral chemotherapy candidate.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Oncology
Hao Yang, Kerstin Huebner, Chuanpit Hampel, Katharina Erlenbach-Wuensch, Selva Babu Selvamani, Vikas Shukla, Carol I. Geppert, Arndt Hartmann, Vijayalakshmi Mahadevan, Regine Schneider-Stock
Summary: Our study found that loss of ATF2 leads to 5-FU resistance by activating the DDR pathway. ATF2 binds to ATR to prevent Chk1 phosphorylation, affecting DNA damage repair. Mutant p53 seems to override the tumor suppressor function of ATF2.
Article
Oncology
Zhuhe Luo, Ruijun Chen, Shen Hu, Xibin Huang, Zhenyi Huang
Summary: The study reveals that PVT1 promotes resistance of colon cancer cells to 5-FU by regulating the miR-486-5p/CDK4 axis. This could provide a potential target for overcoming 5-FU resistance in colon cancer therapy.
Article
Biochemistry & Molecular Biology
Malgorzata Milczarek, Anna Pogorzelska, Katarzyna Wiktorska
Summary: Combined treatment with 5-fluorouracil and 2-oxohexyl isothiocyanate acts synergistically in colon cancer by enhancing cytotoxic activity, primarily through the apoptotic process.
Article
Oncology
Marzia Spagnardi, Jenny Paredes, Jovanny Zabaleta, Jone Garai, Tiana Reyes, Laura A. Martello, Jennie L. Williams
Summary: This study found differential expression of inflammatory genes and proteins between African American and Caucasian American colon cancer cell lines. Increased expression of inflammatory genes may contribute to reduced response to the chemotherapeutic agent 5-FU in African American patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Chemistry, Physical
Jaya R. Lakkakula, Rui Werner Macedo Krause, Deepika Divakaran, Sagar Barage, Rohit Srivastava
Summary: The circulation time and solubility of 5-Fluorouracil have been prolonged and increased by complexing it with cyclodextrin and conjugating it onto gold nanoparticles to form 5Fu ICAu. The 5Fu-ICAu system showed higher cell inhibition rate on breast cancer cell lines due to the enhanced EPR effect allowing selective accumulation of nanoparticles at tumor sites.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Pharmacology & Pharmacy
Sidika Genc, Ali Taghizadehghalehjoughi, Yesim Yeni, Abbas Jafarizad, Ahmet Hacimuftuoglu, Dragana Nikitovic, Anca Oana Docea, Yaroslav Mezhuev, Aristidis Tsatsakis
Summary: In this study, the combination of Fe3O4 NPs and 5-FU showed a synergistic cytotoxic effect on Caco-2 cells at concentrations below the active drug threshold levels.
Article
Oncology
Yuxin Liu, Swati Bhardwaj, Keith Sigel, John Winters, Joseph Terlizzi, Michael M. Gaisa
Summary: This study investigated the prevalence and severity of anal HPV disease among MSM LWH under the age of 35, finding a high prevalence of HPV infection and precancer but no cases of invasive anal cancer. This supports the adoption of age-based anal cancer screening for this population.
INTERNATIONAL JOURNAL OF CANCER
(2024)
Correction
Oncology
J. Gu, S. Xie, S. Wang
INTERNATIONAL JOURNAL OF CANCER
(2024)