期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 79, 期 -, 页码 527-532出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2015.03.057
关键词
Lactobacillus acidophilus; Surface layer protein; Murein hydrolase; Peptidoglycan; Sublethal
资金
- National Natural Science Foundation of China [31471696]
- 863 Project [2013AA102207]
- Twelfths 5-year National Key Technology R&D Program of China [2012BAD33B05]
The aim of this study was to investigate the murein hydrolase activities of the surface layer proteins (SLPs) from two strains of Lactobacillus acidophilus using zymography. The influence of these hydrolase activities on Escherichia coli ATCC 43893 was also evaluated by analysing their growth curve, cell morphology and physiological state. After the incubation of E. coli with SLPs, growth was inhibited, the number of viable cells was significantly reduced, examination by transmission electron microscopy showed that the cell wall was damaged and flow cytometry results indicated that the majority of the cells were sublethally injured. All of these results suggested that the SLPs of both L. acidophilus strains possessed murein hydrolase activities that were sublethal to E. coli cells. (C) 2015 Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据