期刊
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
卷 34, 期 2, 页码 251-261出版社
WILEY
DOI: 10.1111/j.1530-0277.2009.01088.x
关键词
Liver; Alcohol; Knockout Mice; Copper/Zinc Superoxide Dismutase
资金
- National Institute of Alcohol Abuse and Alcoholism [1 F31 AA017045-01, AA09384]
- U.S. Department of Veterans Affairs
Background: Ethanol metabolism increases production of reactive oxygen species, including superoxide (O2(center dot-)) in the liver, resulting in significant oxidative stress, which causes cellular damage. Superoxide dismutase (SOD) is an antioxidant enzyme that converts superoxide to less toxic intermediates, preventing O2(center dot-) accumulation. Because the absence of SOD would confer less resistance to oxidative stress, we determined whether damage to hepatic proteolytic systems was greater in SOD-/- than in SOD+/+ mice after chronic ethanol feeding. Methods: Female wild-type (SOD+/+) and Cu/Zn-SOD knockout (SOD-/-) mice were pair-fed ethanol and control liquid diets for 24 days, after which liver injury was assessed. Results: Ethanol-fed SOD-/- mice had 4-fold higher blood ethanol, 2.8-fold higher alanine aminotransferase levels, 20% higher liver weight, a 1.4-fold rise in hepatic protein levels, and 35 to 70% higher levels of lipid peroxides than corresponding wild-type mice. While wild-type mice exhibited fatty liver after ethanol administration, SOD-/- mice showed no evidence of ethanol-induced steatosis, although triglyceride levels were elevated in both groups of knockout mice. Ethanol administration caused no significant change in proteasome activity, but caused lysosomal leakage in livers of SOD-/- mice but not in wild-type mice. Alcohol dehydrogenase activity was reduced by 50 to 60% in ethanol-fed SOD-/- mice compared with all other groups. Additionally, while ethanol administration induced cytochrome P450 2E1 (CYP2E1) activity in wild-type mice, it caused no such induction in SOD-/- mice. Unexpectedly, ethanol feeding significantly elevated total and mitochondrial levels of glutathione in SOD knockout mice compared with wild-type mice. Conclusion: Ethanol-fed SOD-/- mice exhibited lower alcohol dehydrogenase activity and lack of CYP2E1 inducibility, thereby causing decreased ethanol metabolism compared with wild-type mice. These and other atypical responses to ethanol, including the absence of ethanol-induced steatosis and enhanced glutathione levels, appear to be linked to enhanced oxidative stress due to lack of antioxidant enzyme capacity.
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