Article
Biochemistry & Molecular Biology
Stefano Stabilini, Anna Menini, Simone Pifferi
Summary: TMEM16F is involved in various physiological processes and acts as both a Ca2+-dependent phospholipid scramblase and a Ca2+-activated ion channel. The ion selectivity of TMEM16F-mediated current depends on the recording configuration, with higher permeability for Na+ in inside-out experiments and a slight preference for Cl over bar permeation in whole-cell recordings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Johann Biedermann, Sebastian Braunbeck, Andrew J. R. Plested, Han Sun
Summary: Fast excitatory synaptic transmission in the central nervous system relies on the AMPA-type glutamate receptor (AMPAR), which incorporates a nonselective cation channel. Recent studies combining molecular dynamic simulations and electrophysiology have revealed the ion permeation mechanisms of AMPA receptors, showing that Na+, K+, and Cs+ can permeate at physiological rates through a simple selectivity filter structure. The permissive architecture of the filter allows for rapid, nonselective cation permeation and copermeation by water, with Cs+ slightly more permeant than Na+ due to preferential binding sites.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Christian J. G. Tessier, Raymond M. Sturgeon, Johnathon R. Emlaw, Gregory D. McCluskey, F. Javier Perez-Areales, Corrie J. B. daCosta
Summary: Human adult muscle-type acetylcholine receptors are heteropentameric ion channels formed from different subunits, but they share a common ancestral homopentameric origin. A reconstructed ancestral muscle-type beta-subunit can form spontaneously open homopentameric ion channels, demonstrating that the function of the receptor is independent of agonist and does not require complex heteropentameric architecture.
Article
Multidisciplinary Sciences
Ya-nan Deng, Hamdy Kashtoh, Quan Wang, Guang-xiao Zhen, Qi-yu Li, Ling-hui Tang, Hai-long Gao, Chun-rui Zhang, Li Qin, Min Su, Fei Li, Xia-he Huang, Ying-chun Wang, Qi Xie, Oliver B. Clarke, Wayne A. Hendrickson, Yu-hang Chen
Summary: Stomata in leaves regulate gas exchange between the plant and its atmosphere. Phosphorylation of SLAC1 fine-tunes an equilibrium between basal and activated states, controlling the degree of stomatal opening. Cryo-EM analysis revealed structural features of SLAC1 that help understand its functional mechanism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Gregory C. Amberg, Ji Yeon Lee, Sang Don Koh, Kenton M. Sanders
Summary: A-type currents, rapidly inactivating voltage-gated potassium currents, were identified in smooth muscle cells (SMCs) isolated from murine fundus, with robust peak amplitudes averaging 1.5 nA at 0 mV. These currents are likely active at physiological membrane potentials and contribute to the negative membrane potentials of this tonic smooth muscle. Kv4 channels are likely responsible for generating the A-type current in murine fundus SMCs, as confirmed by quantitative analysis and immunohistochemistry.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yevheniia Kravenska, Vanessa Checchetto, Ildiko Szabo
Summary: Potassium ions can enter and exit mitochondria through various channels in the outer and inner membranes, with the ATP-dependent potassium channel being the most studied and playing a crucial role in protecting cells against ischemic and neuronal damage.
Article
Parasitology
Evgeny G. Chulkov, Elena Isaeva, Cheryl L. Stucky, Jonathan S. Marchant
Summary: The parasitic flatworm ion channel, TRPMPZQ, is activated by praziquantel (PZQ) and membrane stretch, making it a multimodal ion channel. Conservation of TRPMPZQ suggests its importance in flatworm physiology. The mechanosensitivity of TRPMPZQ is significant for schistosome physiology due to recurring pressures and mechanical cues.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2023)
Article
Pharmacology & Pharmacy
Martina Kaniakova, Jan Korabecny, Kristina Holubova, Lenka Kleteckova, Marketa Chvojkova, Kristina Hakenova, Lukas Prchal, Martin Novak, Rafael Dolezal, Vendula Hepnarova, Barbora Svobodova, Tomas Kucera, Katarina Lichnerova, Barbora Krausova, Martin Horak, Karel Vales, Ondrej Soukup
Summary: NMDARs are a potential target for the treatment of neurodegenerative disorders, but drugs targeting them often come with specific psychotic side effects and abuse potential. The compound 7-PhO-THA, with dual inhibitory effects on AChE and NMDARs, shows promise as a therapeutic option.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Peiwen Li, Xiaohui Tian, Gongxin Wang, Enshe Jiang, Yanming Li, Guoliang Hao
Summary: In this study, it was found that osimertinib can cause prolongation of the QT interval, increased conduction time, and inhibition of channels. These findings might be the main cause of the cardiotoxicity effects induced by the drug.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Parasitology
Evgeny G. Chulkov, Oleg Palygin, Nawal A. Yahya, Sang-Kyu Park, Jonathan S. Marchant
Summary: Ion channels have been identified as effective targets for anthelmintic chemotherapy, including a recently discovered flatworm ion channel targeted by praziquantel (PZQ) for the treatment of schistosomiasis. This study provides a detailed biophysical characterization of the properties of the PZQ-activated ion channel in various parasitic flatworms, identifying its distinctive features and calcium permeability. The findings contribute to a better understanding of the mechanism of action of PZQ and pave the way for future investigations into the regulation of this novel antiparasitic target.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2023)
Article
Biology
Xinle Zou, Sri Karthika Shanmugam, Scott A. Kanner, Kevin J. Sampson, Robert S. Kass, Henry M. Colecraft
Summary: The study investigated the reconstitution of PKA regulation of I-Ks in the absence of AKAP9 using a targeted protein phosphorylation approach. The results showed that targeted recruitment of endogenous Ca to E1-YFP enhanced I-Ks while recruitment to Q1-YFP inhibited IKs. Proteomic analysis revealed different phosphorylation sites modified by Ca targeting Q1-YFP compared to free Ca. This study provides insights into the regulation of IKs by phosphorylation and suggests a potential approach to prevent exercise-induced sudden cardiac death.
Article
Neurosciences
Eder Gambeta, Maria A. Gandini, Ivana A. Souza, Laurent Ferron, Gerald W. Zamponi
Summary: A novel missense mutation in the CACNA1A gene was identified in a patient with trigeminal neuralgia, which alters the gating properties of the Ca(V)2.1 calcium channel. The mutation leads to changes in the voltage-dependence of activation, inactivation, and calcium-dependent inactivation, suggesting a gain of function effect that may contribute to the development of trigeminal neuralgia.
Article
Multidisciplinary Sciences
Dominic G. Whittaker, Jiahui Wang, Joseph G. Shuttleworth, Ravichandra Venkateshappa, Jacob M. Kemp, Thomas W. Claydon, Gary R. Mirams
Summary: Mathematical models of voltage-gated ion channels play a crucial role in research and applications, but determining the appropriate complexity and structure of these models is challenging. This study utilizes the MBAM method to simplify ion channel models, resulting in models with fewer parameters while still retaining good predictive capability.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)
Review
Pharmacology & Pharmacy
Shirin Ahmadi, Melisa Benard-Valle, Kim Boddum, Fernanda C. Cardoso, Glenn F. King, Andreas Hougaard Laustsen, Anne Ljungars
Summary: Ion channels are crucial for various physiological processes. Venomous creatures exploit ion channels by producing toxins that target them, leading to dysregulation which can cause severe complications in humans. However, the discovery of selective venom toxins has contributed to the development of drugs for envenoming and ion channel-related diseases.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Cell Biology
Gui-Lan Chen, Jian Li, Jin Zhang, Bo Zeng
Summary: Ion channels are the second largest class of drug targets. Cryo-electron microscopy has revolutionized our understanding of ion channel structures and gating mechanisms, but also raised concerns about the pore-forming ability of some previously identified channel proteins. Cross-validation by electrophysiological and structural analyses is proposed as essential for determining molecular identities of novel ion channels.
Article
Substance Abuse
Heather Trantham-Davidson, L. Judson Chandler
Article
Neurosciences
Jacqueline M. Barker, Daniel H. Lench, L. Judson Chandler
PSYCHOPHARMACOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Samuel W. Centanni, Elizabeth J. Burnett, Heather Trantham-Davidson, L. Judson Chandler
Article
Substance Abuse
Kathryn B. Spencer, Patrick J. Mulholland, L. Judson Chandler
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2016)
Article
Substance Abuse
Elizabeth J. Glover, Molly J. McDougle, Griffin S. Siegel, Thomas C. Jhou, L. Judson Chandler
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2016)
Article
Neurosciences
Heather Trantham-Davidson, Samuel W. Centanni, S. Corrin Garr, Natasha N. New, Patrick J. Mulholland, Justin T. Gass, Elizabeth J. Glover, Stan B. Floresco, Fulton T. Crews, Harish R. Krishnan, Subhash C. Pandey, L. Judson Chandler
NEUROPSYCHOPHARMACOLOGY
(2017)
Article
Clinical Neurology
Elizabeth J. Burnett, L. Judson Chandler, Heather Trantham-Davidson
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY
(2016)
Review
Neurosciences
Patrick J. Mulholland, L. Judson Chandler, Peter W. Kalivas
TRENDS IN NEUROSCIENCES
(2016)
Article
Neurosciences
J. T. Gass, J. T. McGonigal, L. J. Chandler
Article
Pharmacology & Pharmacy
Jacqueline M. Barker, Kathleen G. Bryant, Jennifer I. Osborne, L. J. Chandler
FRONTIERS IN PHARMACOLOGY
(2017)
Review
Substance Abuse
Fulton T. Crews, Donita L. Robinson, L. Judson Chandler, Cindy L. Ehlers, Patrick J. Mulholland, Subhash C. Pandey, Zachary A. Rodd, Linda P. Spear, H. Scott Swartzwelder, Ryan P. Vetreno
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
(2019)
Article
Substance Abuse
Jacqueline M. Barker, Kathleen G. Bryant, Alan Montiel-Ramos, Benjamin Goldwasser, Lawrence Judson Chandler
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2020)
Article
Neurosciences
Jacqueline M. Barker, W. Bailey Glen, David N. Linsenbardt, Christopher C. Lapish, L. Judson Chandler
Article
Substance Abuse
Jennifer A. Rinker, Diana B. Fulmer, Heather Trantham-Davidson, Maren L. Smith, Robert W. Williams, Marcelo F. Lopez, Patrick K. Randall, L. Judson Chandler, Michael F. Miles, Howard C. Becker, Patrick J. Mulholland
Article
Neurosciences
Jacqueline M. Barker, Kathleen G. Bryant, L. Judson Chandler
Article
Substance Abuse
Atefeh Bakhtazad, Afsaneh Asgari Taei, Fatemeh Parvizi, Mehdi Kadivar, Maryam Farahmandfar
Summary: This study found that repeated morphine pretreatment can reverse the impairment of spatial learning and memory caused by ethanol, and this effect may be explained by the modulation of CaMKII and BDNF expressions in the hippocampus and amygdala.