Article
Biochemistry & Molecular Biology
Christian M. Gallardo, Shiyi Wang, Daniel J. Montiel-Garcia, Susan J. Little, Davey M. Smith, Andrew L. Routh, Bruce E. Torbett
Summary: This study overcomes technical challenges in RNA virus sequencing by developing a nanopore-based method to accurately sequence viral genomes from clinical samples. The method can detect rare viral genomes at <1% frequency, facilitating the study of genetic resistance to antiviral therapy and immune pressure.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Infectious Diseases
Dorcas Maruapula, Natasha O. Moraka, Ontlametse T. Bareng, Patrick T. Mokgethi, Wonderful T. Choga, Kaelo K. Seatla, Nametso Kelentse, Catherine K. Koofhethille, Boitumelo J. L. Zuze, Tendani Gaolathe, Molly Pretorius-Holme, Joseph Makhema, Vlad Novitsky, Roger Shapiro, Sikhulile Moyo, Shahin Lockman, Simani Gaseitsiwe
Summary: This study evaluated the prevalence of archived RVP-RAMs in people living with HIV and found that both ART-naive and ART-suppressed individuals had RVP-RAMs. The most common RVP-RAM was E138A. Monitoring RVP-RAMs is crucial for effective use of cabotegravir/rilpivirine in this setting.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Mijael Rozenszajn, Solange Arazi-Caillaud, Moira Taicz, Rosa Bologna, Andrea Mangano, Paula C. Aulicino
Summary: Baseline genotyping for HIV-1 in vertically infected children is crucial for personalized medicine. Children with PDR require closer monitoring of their first-line ART regimens to prevent early virological failure and resistance accumulation.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Yuan Zong, Yijing Liu, Chenxiao Xue, Boshu Li, Xiangyang Li, Yanpeng Wang, Ji Li, Guanwen Liu, Xingxu Huang, Xiaofeng Cao, Caixia Gao
Summary: In this study, we have improved the editing efficiency of prime editing by engineering the structure of the prime editor and combining it with specific guide RNAs. The optimized prime editor showed significantly increased editing efficiency in plant cells, and when combined with engineered guide RNAs, it resulted in the generation of herbicide-tolerant rice plants.
NATURE BIOTECHNOLOGY
(2022)
Article
Infectious Diseases
Charlotte Charpentier, Alexandre Storto, Cathia Soulie, Valentine Marie Ferre, Marc Wirden, Veronique Joly, Sidonie Lambert-Niclot, Romain Palich, Laurence Morand-Joubert, Roland Landman, Karine Lacombe, Christine Katlama, Jade Ghosn, Anne-Genevieve Marcelin, Vincent Calvez, Diane Descamps
Summary: The study found that HIV-1 subtypes A6/A1 and the presence of rilpivirine RAMs are baseline risk factors associated with virological failure of cabotegravir+rilpivirine dual therapy among ARV-naive patients. Among large sequence databases, approximately 10.1% of patients would be ineligible for cabotegravir+rilpivirine dual therapy due to the presence of rilpivirine-resistant viruses and HIV-1 subtype A6/A1 sequences.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Microbiology
Jerry L. Jeffrey, Marty St Clair, Ping Wang, Chunfu Wang, Zhufang Li, Jagadish Beloor, Christine Talarico, Robert Fridell, Mark Krystal, C. Thomas White, Sandy Griffith, Ronald D'Amico, Kimberly Smith, Veerle Van Eygen, Johan Vingerhoets, Kati Vandermeulen, William Spreen, Jan van Lunzen
Summary: The FLAIR study demonstrated the noninferiority of monthly long-acting cabotegravir + rilpivirine compared to daily oral dolutegravir/abacavir/lamivudine in maintaining virologic suppression.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Virology
Mattia Trunfio, Carmela Pinnetti, Stefania Arsuffi, Francesca Bai, Luigi Celani, Gabriella D'Ettorre, Jaime H. H. Vera, Antonella D'Arminio Monforte, Emanuele Foca, Valeria Ghisetti, Stefano Bonora, Andrea Antinori, Andrea Calcagno
Summary: The use of specific antiretroviral (ARV) classes, such as protease inhibitors, is associated with a higher risk of cerebrospinal fluid escape (CVE). This study investigated whether archived resistance-associated mutations (RAMs) mediate this relationship in patients treated with incompletely active ARV regimens. The presence of RAMs in the reverse transcriptase gene was associated with an increased risk of CVE.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Chemistry, Medicinal
Dongwei Kang, Yanying Sun, Da Feng, Shenghua Gao, Zhao Wang, Lanlan Jing, Tao Zhang, Xiangyi Jiang, Hao Lin, Erik De Clercq, Christophe Pannecouque, Peng Zhan, Xinyong Liu
Summary: This article reports the design, synthesis, structure-activity relationship studies, antiviral activity, enzyme inhibition, and druggability evaluation of dihydrofuro[3,4-d]pyrimidine derivatives as a potent class of HIV-1 non-nucleoside reverse transcriptase inhibitors (NNRTIs). Compounds 14b and 16c exhibited superior potency against a panel of HIV-1-resistant strains, especially for specific mutations. They also showed moderate RT enzyme inhibition and favorable pharmacokinetic and safety properties, making them promising candidates for further development.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Dongwei Kang, Yanying Sun, Da Feng, Shenghua Gao, Zhao Wang, Lanlan Jing, Tao Zhang, Xiangyi Jiang, Hao Lin, Erik De Clercq, Christophe Pannecouque, Peng Zhan, Xinyong Liu
Summary: In this study, dihydrofuro[3,4-d]pyrimidine derivatives were designed, synthesized and evaluated as potent HIV-1 non-nucleoside reverse transcriptase inhibitors. Compounds 14b and 16c exhibited superior antiviral activity and RT enzyme inhibition, making them promising leads for further development.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jingxuan Qiu, Xinxin Tian, Jiangru Liu, Yulong Qin, Junjie Zhu, Dongpo Xu, Tianyi Qiu
Summary: The study utilized a novel proteochemometric model to predict drug resistance of HIV-1 nucleoside reverse transcriptase inhibitors against reverse transcriptase variants. The model showed high accuracy on external validation and revealed characteristics of drug-resistant sequences.
Article
Chemistry, Organic
Matteo A. . Tallarida, Fabrizio Olivito, Claudio D. Navo, Vincenzo Algieri, Antonio Jiritano, Paola Costanzo, Ana Poveda, Maria J. Moure, Jesus Jimenez-Barbero, Loredana Maiuolo, Gonzalo Jimenez-Oses, Antonio De Nino
Summary: The synthesis of polysubstituted spirocyclopropyl oxindoles using rare-earth metal (REM) salts, particularly Sc(OTf)3, was achieved with high diastereoselectivity (<= 94:6:0:0) in a multicomponent reaction. Density functional theory calculations on the model reaction confirmed the observed selectivity and demonstrated that the coordinating capabilities and oxophilicity of the metal played a crucial role in inducing the formation of one main diastereoisomer.
Article
Chemistry, Medicinal
Xiangyi Jiang, Boshi Huang, Waleed A. Zalloum, Chin-Ho Chen, Xiangkai Ji, Zhen Gao, Jiaojiao Dai, Minghui Xie, Dongwei Kang, Erik De Clercq, Christophe Pannecouque, Xinyong Liu, Peng Zhan
Summary: Novel diarypyrimidine derivatives were designed based on previously reported HIV-1 NNRTIs BH-11c and XJ-10c to improve antiresistance and drug-like profiles. Compound 12g showed the highest inhibitory activity against wild-type and NNRTI-resistant HIV-1 strains, with EC50 values ranging from 0.024 to 0.0010 μM. Its improved antiresistance profile compared to ETR was explained by MD simulation study and it also showed improved water solubility and other drug-like properties. Compound 12g exhibited promising pharmacokinetics parameters and could be a potential lead compound for new antiretroviral drug development.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Zhao Wang, Waleed A. Zalloum, Wenbo Wang, Xiangyi Jiang, Erik De Clercq, Christophe Pannecouque, Dongwei Kang, Peng Zhan, Xinyong Liu
Summary: Compound 20a, designed based on structural biology information, shows remarkable antiviral activity against HIV-1, low toxicity, high safety index, improved solubility, and promising drug-like properties. It has the potential to be a valuable drug candidate.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Infectious Diseases
Szu-Wei Huang, Mei-Chen Shen, Wen-Hung Wang, Wei-You Li, Jen-Hsien Wang, Cheng-Yin Tseng, Po-Yu Liu, Lih-Shinn Wang, Yu-Lin Lee, Yi-Ming Arthur Chen, Chun-Yuan Lee, Po-Liang Lu, Sheng-Fan Wang
Summary: The prevalence of TDR among HIV-1-infected patients in Taiwan is increasing, with NNRTI-related mutations being the most common. A higher baseline HIV-1 viral load and receiving InSTI-based regimens are associated with shorter time to virological failure or longer time to viral suppression in this population.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Chemistry, Medicinal
Yanying Sun, Zhenzhen Zhou, Da Feng, Lanlan Jing, Fabao Zhao, Zhao Wang, Tao Zhang, Hao Lin, Hao Song, Erik De Clercq, Christophe Pannecouque, Peng Zhan, Xinyong Liu, Dongwei Kang
Summary: A series of novel diarylpyrimidine derivatives were generated using a cocrystal structure-based drug design strategy to discover new HIV-1 NNRTIs with increased drug resistance profiles and improved PK properties. Among them, compound 36a exhibited outstanding antiviral activity and higher binding affinity to HIV-1 RT. Molecular docking and simulation were performed to rationalize the design and improved drug resistance. Compound 36a·HCl showed favorable PK and safety properties, suggesting it as a potential drug candidate for anti-HIV-1 therapy.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Public, Environmental & Occupational Health
Rishma Amarsy, Sandra Fournier, David Trystram, Catherine Monteil, Xavier Raynaud, Vincent Jarlier, Jerome Robert
Summary: The impact of COVID-19 pandemic on bloodstream infections due to Streptococcus pneumoniae and Streptococcus pyogenes was assessed in 25 university hospitals in Paris. The incidence rates of BSIs, which had been stable in 2018 and 2019, decreased for the two pathogens during the two lockdown periods in 2020. Containment policies such as social distancing, masking, and hand hygiene strengthening in community and hospital settings are likely to reduce BSIs caused by these pathogens.
AMERICAN JOURNAL OF INFECTION CONTROL
(2023)
Article
Infectious Diseases
Romain Palich, Sonia Burrel, Gentiane Monsel, Agathe Nouchi, Alexandre Bleibtreu, Sophie Seang, Vincent Berot, Cecile Brin, Ariane Gavaud, Yara Wakim, Nagisa Godefroy, Antoine Faycal, Yanis Tamzali, Thomas Grunemwald, Michel Ohayon, Eve Todesco, Valentin Leducq, Stephane Marot, Vincent Calvez, Anne-Genevieve Marcelin, Valerie Pourcher
Summary: This study describes the distribution of Monkeypox virus (MPXV) in the human body, and the results show that the virus is more frequently detected and has higher viral loads in the skin, anus, and throat. These findings suggest that transmission most likely occurs through direct body contact.
LANCET INFECTIOUS DISEASES
(2023)
Article
Immunology
Adolfo de Salazar, Laura Vinuela, Ana Fuentes, Elisa Teyssou, Charlotte Charpentier, Sidonie Lambert-Niclot, Esther Serrano-Conde, Marta Pingarilho, Lavinia Fabeni, Anne De Monte, Karl Stefic, Carlo Federico Perno, Antonio Aguilera, Iker Falces, Rafael Delgado, Sandra Fernandes, Isabel Diogo, Perpetua Gomes, Dimitrios Paraskevis, Maria-Mercedes Santoro, Francesca Ceccherini-Silberstein, Anne-Genevieve Marcelin, Federico Garcia
Summary: The prevalence of transmitted drug resistance to INSTIs and NRTIs and clinically relevant resistance was evaluated in newly diagnosed HIV patients naive to ART in Europe. The results showed low prevalence rates of INSTI-SDRMs (0.30%), NRTI-SDRMs (5.77%), INSTI-CRR (2.33%), and first-line NRTI-CRR (1.74-2.29%) during 2018-2021.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Guillaume Martin-Blondel, Anne-Genevieve Marcelin, Cathia Soulie, Sofia Kaisaridi, Clovis Lusivika-Nzinga, Karen Zafilaza, Celine Dorival, Laura Nailler, Anais Boston, Anne-Marie Ronchetti, Clea Melenotte, Andre Cabie, Christophe Choquet, Albert Trinh-Duc, Karine Lacombe, Geraldine Gaube, Francois Coustilleres, Valerie Pourcher, Jean-Philippe Martellosio, Nathan Peiffer-Smadja, Marie Chauveau, Pierre Housset, Lionel Piroth, Mathilde Devaux, Gilles Pialoux, Aurelie Martin, Vincent Dubee, Jerome Frey, Audrey Le Bot, Charles Cazanave, Philippe Petua, Roland Liblau, Fabrice Carrat, Youri Yordanov
Summary: This study compared the clinical and virological outcomes in Omicron BA.1 and BA.2-infected patients who received sotrovimab and nirmatrelvir for the prevention of severe COVID-19. The results showed that early administration of nirmatrelvir was associated with faster viral clearance compared to sotrovimab, which may contribute to reducing transmission and preventing viral resistance.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Review
Biochemistry & Molecular Biology
Adelie Gothland, Aude Jary, Philippe Grange, Valentin Leducq, Laurianne Beauvais-Remigereau, Nicolas Dupin, Anne-Genevieve Marcelin, Vincent Calvez
Summary: Reprogrammed metabolism, redox equilibrium, and ROS production play important roles in cancer development and are particularly influential in HHV-8 related diseases such as Kaposi sarcoma and primary effusion lymphoma. Primaquine diphosphate has been found to induce apoptosis in HHV-8 infected PEL cell lines through ROS production, indicating that targeting the redox balance could be a potential approach for treating HHV-8 related diseases. This review focuses on the role of ROS in cancer development and provides evidence for the importance of redox pathways in HHV-8 pathologies.
Review
Microbiology
Theophile Cocherie, Karen Zafilaza, Valentin Leducq, Stephane Marot, Vincent Calvez, Anne-Genevieve Marcelin, Eve Todesco
Summary: SARS-CoV-2 variants of concern have evolved through the accumulation of mutations in the Spike protein, which is responsible for viral binding and contains immunodominant epitopes. Different variants have emerged, each with unique characteristics in terms of transmissibility, virulence, and immune escape. The appearance of successive variants has led to the progressive improvement of SARS-CoV-2's transmissibility and its ability to evade the immune system. This review provides a comprehensive summary of the impacts of Spike mutations and the variants of concern.
Article
Infectious Diseases
Daniele Armenia, Maria M. Santoro, Charlotte Charpentier, Ada Bertoli, Federica Forbici, Vincent Calvez, Diane Descamps, Francesca Ceccherini-Silberstein, Anne-Genevieve Marcelin, Philippe Flandre
Summary: This study aimed to evaluate integrase resistance and its predictors in HIV-1 infected individuals who failed a dolutegravir-based antiretroviral therapy. Major resistance mutations and genotypic susceptibility score were analyzed. The results showed that INSTI resistance was low and mainly related to previous first-generation INSTI exposure. Monitoring integrase resistance is crucial to preserve the efficacy of INSTI class in the future.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Letter
Infectious Diseases
Aude Jary, Sokleaph Cheng, Stephane Marot, Gervillien Arnold Malonga, Theophile Cocherie, Steve Wignall, Vincent Calvez, Sophat Phal, Kem Vichet, Anne-Genevieve Marcelin, Gauthier Delvallez
JOURNAL OF INFECTION
(2023)
Article
Virology
Djeneba B. Fofana, Houdou Diarra, Ibrahima Guindo, Mahamadou K. Savadogo, Marceline d'Almeida, Fatoumata I. Diallo, Aliou Balde, Cathia Soulie, Amadou Kone, Anne-Genevieve Marcelin, Almoustapha I. Maiga, Sidonie Lambert-Niclot, Mamoudou Maiga, Sally McFall, Claudia A. Hawkins, Robert L. Murphy, Mariam Sylla, Christine Katlama, Jane L. Holl, Vincent Calvez, Laurence Morand-Joubert
Summary: Limited data exist on INI resistance in children living with HIV/AIDS in West Africa. A study was conducted on HIV-1-infected children in Benin and Mali, and it was found that no major INI resistance mutations were detected in INI-naive patients.
Letter
Infectious Diseases
Elisa Teyssou, Stephane Marot, Adelie Gothland, Isabelle Malet, Karen Zafilaza, Valentin Leducq, Theophile Cocherie, Eve Todesco, Cathia Soulie, Anne-Genevieve Marcelin, Vincent Calvez
JOURNAL OF INFECTION
(2023)
Article
Infectious Diseases
Elisa Teyssou, Stephane Marot, Theophile Cocherie, Antoine Fauchois, Basma Abdi, Eve Todesco, Sepideh Akhavan, Valerie Pourcher, Vincent Calvez, Anne-Genevieve Marcelin, Cathia Soulie
Summary: This study aimed to characterize and compare the viral loads (VL) of the Omicron BA.1 and BA.2 lineages and the Delta variant in newly diagnosed COVID-19 patients. The results showed no significant difference in the median CT value between the Delta variant and the two Omicron lineages during the first diagnostic test. However, the kinetics of CT decreases for the BA.1 and BA.2 lineages were significantly longer than the kinetics for the Delta variant. The BA.2 lineage had a lower median CT value (inversely proportional to the VL) compared to the BA.1 lineage. In conclusion, the BA.2 Omicron lineage presented higher VL at diagnostic and both BA.1 and BA.2 lineages had more prolonged replication compared to the Delta variant.
INFECTIOUS DISEASES NOW
(2023)