Article
Biochemistry & Molecular Biology
Alexander Arsov, Maria Gerginova, Tsvetelina Paunova-Krasteva, Kaloyan Petrov, Penka Petrova
Summary: This study aimed to classify Bacillus thuringiensis strains for broad-spectrum pest control. Twenty strains were isolated from Bulgarian soil, with seven strains containing cry genes. Scanning electron microscopy revealed different crystal shapes produced by these strains. B. thuringiensis serovar galleriae BTG contained the most cry genes. Whole-genome sequencing and annotation of the BTG strain demonstrated its high potential for broad-spectrum insecticide development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Entomology
Alvaro Rodriguez-Gonzalez, Alejandra J. Porteous-Alvarez, Marcos Guerra, Oscar Gonzalez-Lopez, Pedro A. Casquero, Baltasar Escriche
Summary: Xylotrechus arvicola is a destructive pest in vineyards, and the susceptibility of its larvae to five toxic Cry proteins was evaluated. The Cry proteins were found to be effective in controlling the larvae and causing significant alterations in their development.
Article
Entomology
Kyle J. Paddock, Kyle Dellamano, Bruce E. Hibbard, Kent S. Shelby
Summary: Insect resistance to Bacillus thuringiensis (Bt) toxins is a major concern in agriculture, and resistance mechanisms include loss of toxin binding sites, changes in gut microbiota, and midgut tissue regeneration. This study provides histopathological evidence for the first time showing the morphological response of Bt-resistant and Bt-susceptible western corn rootworm larvae to Bt intoxication, shedding light on the multifaceted nature of Bt resistance in these insects.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Agronomy
Haley Kennedy, David L. Kerns, Graham P. Head, Fei Yang
Summary: Our findings show that Vip3Aa-resistant H. zea does not exhibit positive cross-resistance to Cry1 or Cry2 proteins. Furthermore, most pyramided Bt crops have a complete or nearly complete redundant killing effect on Vip3Aa-resistant H. zea. These results suggest that the pyramiding strategy is often effective for managing Vip3Aa resistance in regions of the United States where H. zea has not developed resistance to Cry1 and Cry2 toxins. (c) 2023 Society of Chemical Industry.
PEST MANAGEMENT SCIENCE
(2023)
Review
Chemistry, Analytical
Jiangjiang Gu, Ranfeng Ye, Yiduo Xu, Yashi Yin, Shengqing Li, Hao Chen
Summary: This paper outlines the progress of detection methods for Bt Cry proteins, including traditional methods like ELISA and immunochromatographic strip, as well as novel approaches like electrochemistry and fluorescence. The mechanisms, advantages, limitations, and applications of these methods are discussed, together with highlighting the current challenges and future prospects in this field.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Drishtant Singh, Samiksha, Seema Madhumal Thayil, Satwinder Kaur Sohal, Anup Kumar Kesavan
Summary: Bacillus thuringiensis produces insecticidal proteins that are toxic to insect larvae of economically important crops. In this study, B. thuringiensis was isolated from soil samples in Amritsar, and a protein band of about 95 kDa was identified in one isolate. The purified Cry protein showed significant detrimental effects on lepidopteran and dipteran insect larvae, while having no significant effect on honey bee larvae.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agronomy
Fei Yang, David L. Kerns, Nathan Little, Sebe A. Brown, Scott D. Stewart, Angus L. Catchot, Donald R. Cook, Jeffrey Gore, Whitney D. Crow, Gustav M. Lorenz, Tyler Towles, Bruce E. Tabashnik
Summary: This study evaluated the resistance of Helicoverpa zea, one of the most economically important pests, to Bt toxins and Bt cotton in the United States. The results showed widespread resistance of H. zea to certain Cry toxins produced by Bt cotton and corn, but not to Vip3Aa. The efficacy of Bt cotton decreased with higher survival rates in laboratory bioassays. Rigorous resistance management measures are needed to preserve the efficacy of Vip3Aa against this highly adaptable pest.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Fei Yang, Haley Kennedy, Jose C. Santiago-Gonzalez, David L. Kerns
Summary: The study indicates that cross-pollination in mixed plantings may accelerate the emergence of dual-gene resistance in target insects like Helicoverpa zea to pyramided Bt corn in the USA, ultimately leading to the evolution of resistance to pyramided Bt crops.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Bruce E. Tabashnik, Gopalan Chandran Unnithan, Alexander J. Yelich, Jeffrey A. Fabrick, Timothy J. Dennehy, Yves Carriere
Summary: Pink bollworm shows differential susceptibility to Cry toxins and Vip3Aa, with higher resistance to Cry toxins and higher susceptibility to Vip3Aa in laboratory-selected strains. Cross-resistance between Cry toxins and Vip3Aa is not observed, suggesting the lack of shared midgut receptors. Vip3Aa can be utilized as an effective tool in integrated pest management when used together with novel Cry toxins.
PEST MANAGEMENT SCIENCE
(2022)
Article
Entomology
Bruce E. Tabashnik, Jeffrey A. Fabrick, Yves Carriere
Summary: Genetically engineered crops producing insecticidal proteins have improved pest management, but practical resistance has reduced their efficacy. Resistance has been documented in 11 pest species affecting seven countries, while 16 pest species remain susceptible. Early warnings of resistance have been observed, but further research is needed.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
Bruce E. Tabashnik, Yves Carriere, Yidong Wu, Jeffrey A. Fabrick
Summary: Genetically engineered crops that produce insect-killing proteins have been successful in managing pests, but their effectiveness is reduced when pests develop resistance. This collection of papers presents a global perspective on field-evolved resistance to these crops, including comprehensive summaries of resistance and susceptibility in different pest species and countries. The papers also introduce advanced monitoring techniques and provide multi-year data on resistance to Bt corn. The findings aim to inspire further research and enhance the sustainability of transgenic insecticidal crops.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Jyotsana Tilgam, Kuldeep Kumar, Deepanshu Jayaswal, Sharani Choudhury, Adarsh Kumar, Kuldip Jayaswall, Anil Kumar Saxena
Summary: Transgenic technology using microbial genes has been successful in improving crop characteristics and meeting the growing global demand for food. The acceptance of transgenic crops harboring microbial genes has brought significant changes in society, providing food safety, economic, and health benefits. This approach has shown promising potential in crop improvement and agriculture.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Agronomy
Arun Babu, Dominic D. Reisig, Maiquel P. Pes, Christopher M. Ranger, Narong Chamkasem, Michael E. Reding
Summary: Results from field and laboratory experiments showed that there were no interactions between Bt and chlorantraniliprole in influencing larval survival or growth parameters. Chlorantraniliprole had long-residual effects on H. zea larvae feeding on cotton leaves, but its utility as a Bt-resistance management tool remains unclear.
PEST MANAGEMENT SCIENCE
(2021)
Review
Entomology
Yves Carriere, Bruce E. Tabashnik
Summary: Insect pests are evolving practical resistance to genetically modified crops that produce insect-killing proteins. This study analyzed data to evaluate the association between practical resistance to these crops and two pest traits: fitness costs and incomplete resistance. Lower fitness costs and higher survival rates on genetically modified crops were associated with the evolution of practical resistance. Further research on this resistance syndrome is necessary to sustain the efficacy of these crops.
Article
Food Science & Technology
Bai Xue, Meiling Wang, Zeyu Wang, Changlong Shu, Lili Geng, Jie Zhang
Summary: This study found that extracellular polysaccharides (EPSs) of Bacillus thuringiensis (Bt) play an important role in synergistic activity with Bt toxins. EPSs can enhance the toxicity of Bt toxins to pests and promote their binding to intestinal membranes. EPSs increase the toxicity of Cry1-type toxins and Vip3Aa11 by approximately 2-fold against different lepidopteran pests. The synergistic effects are mediated through the promotion of binding to membranes and protection from digestion.
Article
Integrative & Complementary Medicine
Sinda Fedhila, Wafa Ben Lazhar, Taoufik Jeridi, Vincent Sanchis, Michel Gohar, Didier Lereclus, Jeannette Ben Hamida
AFRICAN JOURNAL OF TRADITIONAL COMPLEMENTARY AND ALTERNATIVE MEDICINES
(2015)
Article
Microbiology
Rafael Patino-Navarrete, Vincent Sanchis
RESEARCH IN MICROBIOLOGY
(2017)
Article
Microbiology
Alexandre Bolotin, Annika Gillis, Vincent Sanchis, Christina Nielsen-LeRoux, Jacques Mahillon, Didier Lereclus, Alexei Sorokin
RESEARCH IN MICROBIOLOGY
(2017)
Editorial Material
Microbiology
Veronique Broussolle, Frederic Carlin, Didier Lereclus, Christina Nielsen-LeRoux, Vincent Sanchis
RESEARCH IN MICROBIOLOGY
(2017)
Article
Microbiology
Rita Kamar, Agnss Rejasse, Isabelle Jehanno, Zaynoun Attieh, Pascal Courtin, Marie-Pierre Chapot-Chartier, Christina Nielsen-Leroux, Didier Lereclus, Laure el Chamy, Mireille Kallassy, Vincent Sanchis-Borja
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
A. Rejasse, N. Gilois, I. Barbosa, E. Huillet, C. Bevilacqua, S. Tran, N. Ramarao, L. P. Stenfors Arnesen, V. Sanchis
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2012)
Article
Microbiology
Rita Kamar, Michel Gohar, Isabelle Jehanno, Agnes Rejasse, Mireille Kallassy, Didier Lereclus, Vincent Sanchis, Nalini Ramarao
JOURNAL OF CLINICAL MICROBIOLOGY
(2013)
Review
Food Science & Technology
Nalini Ramarao, Vincent Sanchis
Article
Microbiology
Nancy Fayad, Rafael Patino-Navarrete, Zakaria Kambris, Mandy Antoun, Mike Osta, Joel Chopineau, Jacques Mahillon, Laure El Chamy, Vincent Sanchis, Mireille Kallassy Awad
CURRENT MICROBIOLOGY
(2019)
Article
Microbiology
Zaynoun Attieh, Carine Mouawad, Agnes Rejasse, Isabelle Jehanno, Stephane Perchat, Ida K. Hegna, Ole A. okstad, Mireille Kallassy Awad, Vincent Sanchis-Borja, Laure El Chamy
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Athanasios Zervas, Marie Ronne Aggerbeck, Henrietta Allaga, Mustafa Guzel, Marc Hendriks, IIona Jonuskiene, Orsolya Kedves, Ayse Kupeli, Janja Lamovsek, Pascal Muelner, Denise Munday, Sahin Namli, Hilal Samut, Ruzica Tomicic, Zorica Tomicic, Filiz Yeni, Raida Zribi Zghal, Xingchen Zhao, Vincent Sanchis-Borja, Niels Bohse Hendriksen
Letter
Microbiology
FEMS MICROBIOLOGY ECOLOGY
(2018)
Article
Microbiology
Marie-Helene Guinebretiere, Valentin Loux, Veronique Martin, Pierre Nicolas, Vincent Sanchis, Veronique Broussolle
GENOME ANNOUNCEMENTS
(2017)
Article
Food Science & Technology
Ana Allende, DecIan Bolton, Marianne Chemaly, Robert Davies, Pablo Salvador, Fernandez Escamez, Rosina Girones, Lieve Herman, Kostas Koutsoumanis, Roland Lindqvist, Birgit Norrung, Antonia Ricci, Lucy Robertson, Giuseppe Ru, Moez Sanaa, Marion Simmons, Panagiotis Skandamis, Emma Snary, Niko Speybroeck, Benno Ter Kuile, John Threlfall, Helene Wahlstrom