期刊
AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
卷 6, 期 31, 页码 6055-6065出版社
ACADEMIC JOURNALS
DOI: 10.5897/AJMR12.434
关键词
Lactobacillus casei subsp rhamnosus CICC 6013; mutation; L-lactic acid; microwave irradiation; amplified fragment length polymorphism
类别
The present study focused on using microwave radiation to produce mutant strains of Lactobacillus rhamnosus with increased lactic acid production. At microwave radiation frequency 2450-MHz and irradiation time 3 min, an L (+)-lactic acid high-yield mutant, W4-3-9, was obtained. With glucose as the sole carbon source, production of lactic acid and L-(+)-lactic acid by the W4-3-9 strain was 1.5-fold greater than that of the parent strain. The mutant was stable for increased L- lactic acid production for at least 9 generations. Results of amplified fragment length polymorphism analysis suggest that mutations of malate/lactate dehydrogenase and pyruvate kinase genes are the possible cause of the increased production of lactic acid in W4-3-9. We believe that microwave irradiation could be an efficient method for inducing microbial mutagenesis.
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