期刊
AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
卷 6, 期 6, 页码 1206-1212出版社
ACADEMIC JOURNALS
DOI: 10.5897/AJMR11.1430
关键词
Probiotics; human peripheral blood mononuclear cell; cytokines; immunoglobulin E; anti-allergy effect
类别
资金
- [HK 95-022]
The aim of this study was to evaluate the immunomodulatory effect of probiotics, namely the production of interferon gamma (IFN-gamma) and interleukin-4 (IL-4) cytokines, in vitro and in vivo. Our experimental groups included ten lactic acid bacterial (LAB) strains, complex strains, a LAB cell free fraction and a control group. Our models included human peripheral blood mononuclear cells (PBMCs) as the human model and BALB/c mice as the animal model. The experiment was carried out over a period of 4 weeks during which the food intake and the body weight of our animal model was reported weekly. BALB/c mice were randomly divided into three groups and injected with 2 mu g/mouse and 6 mu g/mouse ovalbumin (OVA) mixed with complete Freund's adjuvant (CFA) at week zero and two. After week four the serum total immunoglobulin E (IgE) was measured. The results show that probiotic products induced IFN-gamma, suppressed IL-4, and increased the IFN-gamma/IL-4 (Th-1/Th-2) ratio significantly in PBMCs. Probiotic products also decreased significantly the serum total IgE and OVA-specific IgE levels in our animal model. Our study indicates that the multi-species probiotics may therefore have an anti-allergy effect.
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