期刊
ADVANCED SYNTHESIS & CATALYSIS
卷 353, 期 7, 页码 1039-1044出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adsc.201000943
关键词
cross-linked enzyme aggregates (CLEAs); enzyme catalysis; interconversion; peptides; serine protease alcalase
Chemoenzymatic peptide synthesis is potentially the most cost-efficient technology for the synthesis of short and medium-sized peptides with some important advantages. For instance, stoichiometric amounts of expensive coupling reagents are not required and racemisation does not occur rendering purification easier compared to chemical peptide synthesis. In this paper, a novel interconversion reaction of peptide C-terminal alpha-carboxamides into primary alkyl esters with alcalase was used to develop a fully enzymatic peptide synthesis strategy. For each elongation step a cost-efficient amino acid carboxamide building block was used followed by the interconversion of the elongated peptide carboxamide to the corresponding primary alkyl ester. These peptide esters are the starting materials for the next enzymatic peptide elongation step.
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