Article
Nanoscience & Nanotechnology
Sangjun Noh, Francesco Tombola, Peter Burke
Summary: This article describes the concept and roadmap of an engineered electronic nose with the ability to detect analytes that differ by as little as one carbon atom. The strategy involves genetically engineering a fused olfactory odorant receptor (OR) to an ion channel protein, which can electrically detect the binding of a single ligand to a single OR protein domain. The combination of these technological advances and the evolution of OR proteins over millions of years would enable the goal of single molecule sensing with specificity towards analytes that differ by as little as one carbon atom.
Article
Biochemistry & Molecular Biology
Ping Zhu, Shuge Liu, Yulan Tian, Yating Chen, Wei Chen, Ping Wang, Liping Du, Chunsheng Wu
Summary: This study developed an in vivo bioelectronic nose based on a bioengineered rat to detect specific odorants. By recording electrophysiological-responsive signals from the olfactory bulb, different odorants could be successfully classified and detected. The detection limit was low and the method showed promising application potential.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Entomology
Basman H. Al-Jalely, Wei Xu
Summary: The study focused on the olfactory system of Trichogramma pretiosum, identifying 22 putative odorant binding proteins (OBPs) and 105 odorant receptors (ORs) using scanning electron microscopy to observe four types of olfactory sensilla. By examining the expression patterns of OBPs in male and female adults, three female-specific OBPs were identified, suggesting their crucial roles in host-seeking and oviposition behaviors. This research enriches our understanding of T. pretiosum olfactory genes and enhances our knowledge of its olfactory system.
Review
Chemistry, Analytical
Tomasz Wasilewski, Nathalia F. Brito, Bartosz Szulczynski, Marek Wojciechowski, Natalia Buda, Ana Claudia A. Melo, Wojciech Kamysz, Jacek Gebicki
Summary: The detection of biomarkers is crucial for non-invasive medical diagnosis and screening. Reliable disease-specific volatile organic compounds can be identified through analysis of trace level gaseous analytes in breath samples. Biosensors utilizing micro- and nanotechnology advancements show promise in improving the accuracy of diagnosis, but their translation from scientific laboratories to actual clinical diagnostics is still challenging.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Cell Biology
L. Gonzalez-Brusi, J. G. Hamze, I Lamas-Toranzo, M. Jimenez-Movilla, P. Bermejo-Alvarez
Summary: This study aimed to identify sperm proteins interacting with the TMEM95 protein, which is essential for fertilization. Mass spectrometry identified 41 sperm proteins pulled down with TMEM95, suggesting an independent role of TMEM95 in fertilization. Among these proteins, OLFR601 is located in the acrosomal region and may mediate affinity for an odorant involved in fertilization. However, disruption of OLFR601 did not impair sperm fertilization ability, suggesting redundant function with other sperm proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Patrick Marcinek, Franziska Haag, Christiane Geithe, Dietmar Krautwurst
Summary: Molecular recognition plays a key role in biological systems, and the study of pyrazines has led to the discovery of OR5K1 as a specialized olfactory receptor across mammals for the detection of pyrazine-based key food odors and semiochemicals, indicating the significance of ecological evolution.
Article
Biology
Sho Kurihara, Masayoshi Tei, Junichi Hata, Eri Mori, Masato Fujioka, Yoshinori Matsuwaki, Nobuyoshi Otori, Hiromi Kojima, Hirotaka James Okano
Summary: Combined high-field MRI and DTI analyses allow visualization of olfactory sensory neurons and their projections, providing olfactory nerve maps in humans, marmosets, and mice. This technique has the potential to be used clinically for the diagnosis of olfactory dysfunction.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biophysics
Kyung Ho Kim, Dongseok Moon, Jai Eun An, Seon Joo Park, Sung Eun Seo, Siyoung Ha, Jinyeong Kim, Kayoung Kim, Sooyeol Phyo, Jiwon Lee, Hye-Yeon Kim, Moonil Kim, Tai Hyun Park, Hyun Seok Song, Oh Seok Kwon
Summary: In this study, a portable bioelectronic nose (BE-nose) integrated with trace amine-associated receptor (TAAR) nanodiscs (NDs) was developed for food quality monitoring. The BE-nose showed high sensitivity in detecting the biogenic amines cadaverine and putrescine in food samples. Additionally, by integrating commercial gas sensors, the accuracy and reliability of the BE-nose platform for real food samples were improved.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Multidisciplinary Sciences
Rodi Topci, Mihai Alevra, Erik H. U. Rauf, Danielle de Jong-Bolm
Summary: This study investigated the activity of olfactory receptor neurons and their axon terminals in olfactory glomeruli using electrophysiological recordings and rapid 4D calcium imaging. While individual olfactory receptor neurons show diversity in their responses to the same odor stimulus at the soma level, the presynaptic calcium activity in the glomerulus is homogeneous. It was also found that by applying trains of olfactory stimuli with varying amino acid concentrations, it is possible to induce spatio-temporal presynaptic response inhomogeneities and visually distinguish different axon subgroups within the glomerulus.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Applied
Weihong Liu, Yu Zheng, Chen Zhang, Lin Chen, Hanyi Zhuang, Guojun Yao, Hang Ren, Yingjian Liu
Summary: In this study, a biomimetic olfactory recognition system was developed to discriminate the aromas of Chinese liquors. By combining odorant receptors and machine learning, the system demonstrated high accuracy in differentiating liquors of different aroma types, brands, and ageing years. The interactions between liquor aroma compounds and odorant receptors were also elucidated, providing insights into odorant coding at the molecular level.
Article
Biology
Zelia Velez, Rita A. Costa, Wenjing Wang, Peter C. Hubbard
Summary: Ocean acidification may reduce olfactory sensitivity in fish by lowering seawater pH and intracellular pH in olfactory receptor neurons, potentially involving carbonic anhydrase.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ryosuke Inoue, Yosuke Fukutani, Tatsuya Niwa, Hiroaki Matsunami, Masafumi Yohda
Summary: Olfactory receptors (ORs) in olfactory sensory neurons mediate olfaction and require the assistance of receptor-transporting proteins (RTPs) for functional expression. This study examined the interactome of RTP1S and OR using proximity biotinylation and revealed the involvement of HSPA6 and STAU2 in the trafficking process of OR through interaction with RTP1S.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Kevin W. Zhu, Shawn D. Burton, Maira H. Nagai, Justin D. Silverman, Claire A. de March, Matt Wachowiak, Hiroaki Matsunami
Summary: This study utilized targeted spatial transcriptomics to map the distribution of olfactory receptors in the murine olfactory bulb and generated a 3D model. The findings revealed a relationship between receptors and odorants, providing significant insights into olfaction.
NATURE COMMUNICATIONS
(2022)
Article
Agronomy
Xueqin Pan, Jun Liu, Xiao Xu, Liwei Zhang, Long Zhang
Summary: Choosing palatable plants is crucial for insect herbivores' survival, especially for restricted-feeders that specialize on few types of plants. However, the definitive strategy and sensory basis for distinguishing between host and nonhost plants remains controversial. In this study, we investigated the olfactory recognition of host plants in migratory locusts that feed on a limited range of plants. By using a novel behavioral paradigm, we found that the palps of locusts are necessary for differentiating between host and nonhost plants. The characteristic odors of the plants determined the behavioral differentiation, and intact palps were required for this process. We also identified the odorant receptors in the palps that modulate the recognition of key volatiles from host and nonhost plants, demonstrating the role of olfactory signaling in food choice.
Article
Biochemistry & Molecular Biology
R. Jason Pitts, Robert M. Huff, Shan Ju Shih, Jonathan D. Bohbot
Summary: The research identifies indolOR genes in mosquitoes, flies, and Culicidae, shedding light on insect chemical ecology and providing targets for novel odor-based tools.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Tae-Hyun Kim, Kyungwon Yang, Minsuk Kim, Hee-Sun Kim, Jihee Lee Kang
Summary: AIM plays a crucial role in inhibiting inflammasome-mediated caspase-1 activation and IL-1 beta and IL-18 production. IL-10 induces AIM expression by regulating STAT3 activity, leading to anti-inflammatory effects.
CELL DEATH & DISEASE
(2021)
Article
Nanoscience & Nanotechnology
Chang-Seuk Lee, Tae Hyun Kim
Summary: MoS2/graphene nanocomposites can be prepared in large quantities through combined high shear mixing and ultrasonication in deionized water, providing a facile and efficient route for large-scale industrial production. These nanocomposites exhibit excellent electrochemical performances in highly sensitive electrochemical sensors.
ACS APPLIED NANO MATERIALS
(2021)
Meeting Abstract
Oncology
Ji Young You, Eun-Gyeong Lee, Soo Jin Park, Byeong Nam Kim, Seeyoun Lee, Eun Sook Lee, So-Youn Jung, Youngmi Kwon, Yun Ju Kim, Chan Wha Lee, Tae Hyun Kim
Meeting Abstract
Oncology
Jae Sik Kim, Kyubo Kim, Wonguen Jung, Kyung Hwan Shin, Seock-Ah Im, Hee-Jun Kim, Yong Bae Kim, Jee Suk Chang, Jee Hyun Kim, Doo Ho Choi, Yeon Hee Park, Dae Yong Kim, Tae Hyun Kim, Byung Ock Choi, Sea-Won Lee, Suzy Kim, Jeanny Kwon, Ki Mun Kang, Woong-Ki Chung, Kyung Su Kim, Won Sup Yoon, Jin Hee Kim, Jihye Cha, Yoon Kyeong Oh
Article
Chemistry, Multidisciplinary
Byeongho Park, Yeon Kyung Cha, Jisung Kwak, Kyeong Seob Hwang, Hyo-Suk Kim, Subeen Park, Yusin Pak, Tai Hyun Park, Hyun Seok Song, Jae Hun Kim
Summary: This study investigates the refractive index and nonlinear properties of two types of photosensitive nanodiscs and demonstrates the modulation of optical signals using these artificial photoreceptor-based waveguides, providing insights into the functioning of visual cells.
Article
Multidisciplinary Sciences
Joonhyuk Son, Daehyun Kim, Jae Yoon Na, Donggoo Jung, Ja-Hye Ahn, Tae Hyun Kim, Hyun-Kyung Park
Summary: Intestinal perforation in preterm infants is a life-threatening condition. This study developed new machine learning models to predict IP in very low birth weight infants, showing excellent performance in predicting necrotizing enterocolitis-associated IP and spontaneous IP.
SCIENTIFIC REPORTS
(2022)
Article
Electrochemistry
Hong Chul Lim, Seung-Joo Jang, Yujin Cho, Hyunju Cho, Gujapaneni Venkataprasad, Venkatachalam Vinothkumar, Ik-Soo Shin, Tae Hyun Kim
Summary: We propose a novel strategy to prepare PEDOT thin films doped with GQD as an electrode active material for an electrochemical biosensor. The PEDOT-GQD was prepared through simultaneous electropolymerization and doping of EDOT using an ionic complex without any additives. The resulting PEDOT-GQD exhibits expanded-coil structure, enhanced intramolecular electron transfer, and interfacial charge transfer, as well as a porous structure with interconnected 3D networks. PEDOT-GQD shows high electrocatalytic activity and performs excellent in the simultaneous detection of ascorbic acid, dopamine, and uric acid, with high sensitivity, selectivity, reproducibility, and stability.
Article
Chemistry, Analytical
Da Eun Oh, Chang-Seuk Lee, Tae Hyun Kim
Summary: This paper proposes a sensitive and selective electrochemical approach for simultaneous and individual determination of seven electroactive biochemical species using a modified electrode with a nanocomposite of Pt nanoparticles and reduced graphene oxides (PtNP/rGO). The PtNP/rGO-GCE exhibits excellent catalytic activity and large surface area, enabling sensitive and selective detection of the seven biochemical species. The sensor also shows satisfactory reproducibility and stability, making it a promising platform for detecting redox-active biochemical species in various samples.
Editorial Material
Electrochemistry
Hong Chul Lim, Seung-Joo Jang, Yujin Cho, Hyunju Cho, Gajapaneni Venkata Prasad, Vinothkumar Venkatachalam, Ik-Soo Shin, Tae Hyun Kim
Summary: This research presents a PEDOT electrode material doped with graphene quantum dots, which exhibits a high electrochemically active area and interfacial electron transport rate. The PEDOT-GQD material can simultaneously detect various substances with high selectivity and sensitivity.
Article
Multidisciplinary Sciences
Dong Keon Lee, Jin Hyuk Kim, Jaehoon Oh, Tae Hyun Kim, Myeong Seong Yoon, Dong Jin Im, Jae Ho Chung, Hayoung Byun
Summary: Acute thoracic aortic dissection is a life-threatening disease, and deep learning techniques can improve its diagnostic accuracy.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Venkatachalam Vinothkumar, Rajalakshmi Sakthivel, Shen-Ming Chen, Arumugam Sangili, Tae Hyun Kim
Summary: In this study, a Sr@Mn3O4 anchored graphene oxide (GO) electrocatalyst was developed for the detection of nitrofurantoin (NFT). The synergistic activity of Sr@Mn3O4 with GO enhances the specificity and sensitivity of NFT detection. The fabricated Sr@Mn3O4/GO-modified electrode shows a wide dynamic linear range, low limit of detection, and good sensitivity for NFT.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Chemistry, Multidisciplinary
Kyung Ho Kim, Seongjae Jo, Sung Eun Seo, Jaemin Kim, Dae-Sik Lee, Siyeon Joo, Jiwon Lee, Hyun Seok Song, Hee Gu Lee, Oh Seok Kwon
Summary: In this study, a graphene-encased gold nanorod (Gr@AuNR)-based nanoplasmonic sensor is developed for detecting cadaverine gas. The sensor exhibits ultra-high sensitivity and selective capturing capability, with the detection limit increased by applying an external voltage. Experimental results demonstrate the practicality of the sensor in detecting cadaverine gas generated from spoiled pork.
Article
Chemistry, Physical
Rajaram Pandiyan, Venkatachalam Vinothkumar, Shen -Ming Chen, Gajapaneni Venkata Prasad, Tae Hyun Kim
Summary: Perovskite rare earth nickelates, specifically perovskite lanthanum nickelate (LNO), were synthesized and used for the electrochemical sensing of trifluoperazine (TFP) in this study. The crystal structure and surface morphology of LNO were characterized, and its modified screen printed carbon electrode (LNO/SPCE) showed high sensitivity and a broad linear range for TFP detection. The LNO sensor exhibited good anti-interfering ability, stability, reproducibility, and repeatability, and successfully detected TFP in urine and serum samples with satisfactory recovery rates.
SURFACES AND INTERFACES
(2023)
Article
Chemistry, Analytical
Da Eun Oh, Chang-Seuk Lee, Tae Wan Kim, Seob Jeon, Tae Hyun Kim
Summary: Researchers developed a transparent and flexible electrochemical sensor for the non-enzymatic detection of hydrogen peroxide (H2O2) released from living cells. The sensor utilized a platform based on single-walled carbon nanotubes (SWCNTs) decorated with platinum nanoparticles (PtNPs) on a poly(ethylene terephthalate) (PET) substrate. The PtNP/SWCNT/PET sensor exhibited enhanced electrocatalytic activity, good selectivity, stability, and reproducibility, with a wide dynamic range and low detection limit.
Article
Nanoscience & Nanotechnology
Pitchaimani Veerakumar, Arumugam Sangili, Shen-Ming Chen, Venkatachalam Vinothkumar, Tae Hyun Kim
Summary: Ultrathin three-dimensional porous carbon nanosheets derived from Moringa oleifera barks were used as the carbon precursor in this study. Through a simple one-step pyrolysis technique, hierarchical PCN was generated using high temperature pyrolysis and KOH activation. Pt-Ni bimetallic nanoparticles were decorated on the ultrathin 3D PCN using the microwave-solvothermal approach. The Pt-Ni@PCN nanocomposite exhibited excellent electrochemical sensor performance for detecting dihydroxybenzene isomers.
ACS APPLIED NANO MATERIALS
(2023)
