Article
Medicine, Research & Experimental
Ruihao Yang, Shiyan Fu, Ruidong Li, Lei Zhang, Zhigang Xu, Yang Cao, Hongjuan Cui, Yuejun Kang, Peng Xue
Summary: ROS have potential in eliminating tumor cells in situ, with the help of SF for synthesis of metallic nanocrystals. The nanozyme developed from this process showed antitumor effects by continuously supplying H2O2 in vitro and depleting GSH intracellularly.
Article
Engineering, Biomedical
Honglian Yu, Mengting He, Yongcan Li, Yuhan Liu, Zhigang Xu, Lei Zhang, Yuejun Kang, Peng Xue
Summary: Intracellular redox dyshomeostasis caused by tumor microenvironment modulation is an attractive therapeutic target for cancer management. In this study, a dual plasmonic nanoreactor ASC was developed by immobilizing sulfur defective Cu2-xS nanodots onto silk fibroin-capped Au nanoparticles. ASC effectively alleviates tumor hypoxia and generates singlet oxygen (O-1(2)) by catalase-mimic activity of Cu2-xS and electron scavenging by Au. Moreover, ASC consumes glucose and enhances oxidative damage and energy exhaustion of cancer cells, which can be further boosted by Cu2-xS-catalyzed O-2 self-replenishment in H2O2-rich TME. ASC also promotes M1 macrophage activation and therapy-induced immunogenetic cell death (ICD), leading to systematic tumor elimination.
Article
Engineering, Biomedical
Honglian Yu, Yongcan Li, Zhiyu Zhang, Junjie Ren, Lei Zhang, Zhigang Xu, Yuejun Kang, Peng Xue
Summary: This study developed a nanocarrier based on a metal-organic framework nanosystem, which combined photodynamic therapy and chemodynamic therapy for tumor treatment. By disrupting the redox balance in tumor cells, this strategy significantly enhanced the anti-tumor effect and achieved almost complete tumor eradication in vivo.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Zhi-Chao Hu, Jia-Qi Lu, Tai-Wei Zhang, Hai-Feng Liang, Hao Yuan, Di-Han Su, Wang Ding, Rui-Xian Lian, Yu-Xiang Ge, Bing Liang, Jian Dong, Xiao-Gang Zhou, Li-Bo Jiang
Summary: The electrical microenvironment plays a crucial role in bone repair, but the mechanism by which electrical stimulation promotes bone regeneration is unclear. In this study, an electroactive hydrogel based on regenerated silk fibroin (RSF) and MXene nanosheets was developed to efficiently promote bone regeneration. The hydrogel also functioned as a piezoresistive pressure transducer for monitoring the electrophysiological microenvironment.
BIOACTIVE MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Hui Niu, Jiarui Xiao, Xiaoli Lou, Lingling Guo, Yongsheng Zhang, Runhuai Yang, Hao Yang, Shouli Wang, Fuzhou Niu
Summary: This study utilized natural silk fibroin and chitosan to fabricate 3D microscaffolds with good biocompatibility for cancer cell culture and drug sensitivity testing. The scaffolds were characterized using various testing methods and showed promising results in facilitating cell growth and drug sensitivity screening.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Francesca Agostinacchio, Xuan Mu, Sandra Dire, Antonella Motta, David L. Kaplan
Summary: In situ 3D printing is an emerging technique designed for patient-specific needs and performed directly in the patient's tissues in the operating room. Silk fibroin inks are emerging as a viable option due to their diverse range of formulations, aqueous processability, robust and tunable mechanical properties, and self-assembly via biophysical adsorption.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Polymer Science
Juo Lee, Sangbae Park, Sungmin Lee, Hae Yong Kweon, You-Young Jo, Jungsil Kim, Jong Hoon Chung, Hoon Seonwoo
Summary: This study addresses the limitations of using natural polymers such as silk fibroin (SF) in 3D bioprinting by developing silk fibroin-based thermosensitive bioinks (SF-TPBs). The gelation time and mechanical strength of SF-TPBs can be precisely modulated by manipulating the glycerol content without the need for cross-linkers. Chemical analysis confirms the presence of β-sheet conformation in SF-TPBs regardless of glycerol concentration. Increased glycerol content improves gelation kinetics and results in rheological properties suitable for 3D printing. Overall, SF-TPBs offer promising prospects for realizing the potential of 3D bioprinting using natural polymers.
Article
Biochemistry & Molecular Biology
Monika Rajput, Pritiranjan Mondal, Parul Yadav, Kaushik Chatterjee
Summary: Three-dimensional bioprinting based on digital light processing (DLP) provides opportunities for mimicking human tissues. However, the limited availability of biocompatible and biodegradable bioinks hinders progress in this field. This study presents a silk fibroin-derived bioink for DLP bioprinting, which exhibits bone-like viscoelastic behavior and favorable degradation. The bioink supports cell proliferation and osteogenesis. This work highlights the importance of silk fibroin-derived bioinks in tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biochemistry & Molecular Biology
Andy Goebel, Stefania Dell'Endice, Nikolai Jaschke, Sophie Paehlig, Amna Shahid, Lorenz C. Hofbauer, Tilman D. Rachner
Summary: Inflammation plays a critical role in bone metastasis by supporting the survival, migration, invasion, and growth of tumor cells, serving as a key factor in the tumorigenesis and progression of cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Simrit Safarulla, Pritisha S. Khillar, Sudarshan Kini, Amit Kumar Jaiswal
Summary: Prostate cancer bone metastasis is a complex process that occurs in the advanced stages of cancer, requiring further investigation and understanding. 3-D in vitro models provide a convenient platform for studying the development and progression of prostate cancer, aiding in the identification of potential therapeutic targets and testing treatment efficacy.
MATERIALS TODAY COMMUNICATIONS
(2021)
Review
Pharmacology & Pharmacy
Claire L. Ihle, Sabrina J. Wright-Hobart, Philip Owens
Summary: The survival rate of breast cancer patients has improved, but metastatic breast cancer still causes high mortality. Metastasis mainly occurs in the bone, and current treatment methods focus on palliative care and slowing down the progression. Recent advances in molecularly targeted agents have made breakthroughs in the treatment of metastatic breast cancer, but immunotherapies have not been fully applied in estrogen receptor-positive tumors. Therapeutic approaches targeting the bone microenvironment of metastatic breast cancer have potential applications in other bone-resident cancers.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Giriprasath Ramanathan, Grace Felciya Sekar Jeyakumar, Uma Tiruchirapalli Sivagnanam, Pedro Fardim
Summary: In this study, a porous hybrid matrix (CEL-COL-SF) was fabricated using microcrystalline cellulose (CEL), collagen (COL), and silk fibroin (SF). The hybrid matrix exhibited interconnected porous architecture, excellent oxygen permeability, and promoted cell adhesion, proliferation, migration, and minerals deposition. It also showed excellent biocompatibility and cell proliferation when tested with fibroblast and osteoblast-like cells. The matrix demonstrated great potential in bone tissue engineering, as it exhibited the sequential events related to bone regeneration and mimicked the atomic ratio of calcium to phosphate in bone.
PROCESS BIOCHEMISTRY
(2023)
Article
Polymer Science
Chavee Laomeephol, Apichai Vasuratna, Juthamas Ratanavaraporn, Sorada Kanokpanont, Jittima Amie Luckanagul, Martin Humenik, Thomas Scheibel, Siriporn Damrongsakkul
Summary: Binary-blended hydrogels fabricated from Bombyx mori silk fibroin and recombinant spider silk protein showed improved gelation time and cellular interactions compared to individual materials, suggesting potential for biomedical applications. Mixing the two materials enhanced cellular activities, making the hydrogels suitable for various biomedical uses.
Article
Materials Science, Textiles
Zhenyu Xu, Ke Li, Kui Zhou, Shuiyuan Li, Hongwei Chen, Jiaqi Zeng, Rugang Hu
Summary: This study investigates the effects of different concentrations of silk fibroin solutions on the physicochemical and biological properties of silk-hydroxyapatite-sodium alginate scaffolds. The results show that higher concentrations of silk fibroin can promote cell proliferation and meet the requirements for bone tissue repair, providing important reference for bone tissue engineering applications.
FIBERS AND POLYMERS
(2023)
Article
Polymer Science
Zulan Liu, Jiaxuan Wang, Qian Zhang, Zheng Li, Zhi Li, Lan Cheng, Fangyin Dai
Summary: In this study, silk fibroin/graphene nanofiber membranes were prepared using double needle electrospinning, and a three-dimensional composite hierarchy with high sensitivity was obtained by superimposing a monolayer silk fibroin/graphene nanofiber membrane. A pressure sensor was then prepared by encapsulating the three-dimensional hierarchy with polydimethylsiloxane, which exhibited a sensitivity of 7.7 Pa-1, excellent durability (>2000 cycles), and shorter response times (490 ms).
Article
Engineering, Biomedical
Ayna Yazdanpanah, Zahra Madjd, Mohamad Pezeshki-Modaress, Zahra Khosrowpour, Paniz Farshi, Leila Eini, Jafar Kiani, Morteza Seifi, Subhas C. Kundu, Roya Ghods, Mazaher Gholipourmalekabadi
Summary: In this study, a composite of polycaprolactone and gelatin was surface modified with gelatin coating or plasma treatment, and then cultured with fibroblast 3T3 and decellularized to fabricate a fibroblast ECM-conditioned scaffold. The results showed that the plasma-treated scaffold had better cell attachment and ECM secretion, and the conditioned scaffold exhibited improved biocompatibility and angiogenic properties in vivo.
Article
Engineering, Biomedical
David Caballero, Catarina M. Abreu, Ana C. Lima, Nuno N. Neves, Rui L. Reis, Subhas C. Kundu
Summary: Traditional cancer treatment approaches are becoming obsolete, leading to more patients failing to respond to treatments. Personalized genetic profiling offers a more accurate method of treatment, but still faces issues like low local drug efficacy. Efforts are now focused on developing precision medicine-based strategies to improve cancer treatment efficiency.
Article
Engineering, Biomedical
Zahra Khosrowpour, Seyed Mahmoud Hashemi, Samira Mohammadi-Yeganeh, Mehdi Moghtadaei, Peiman Brouki Milan, Lorenzo Moroni, Subhas C. Kundu, Mazaher Gholipourmalekabadi
Summary: The decellularized placental sponge shows potential as a bone substitute and provides a platform for communication between macrophages and mesenchymal stem cells, guiding the differentiation of stem cells towards bone cells.
Review
Engineering, Biomedical
Carlos F. Guimaraes, Daniela Cruz-Moreira, David Caballero, Rogerio P. Pirraco, Luca Gasperini, Subhas C. Kundu, Rui L. Reis
Summary: Microfluidic platforms provide a powerful approach to miniaturize important characteristics of cancers and enhance physiological relevance in vitro testing. Photonics plays a crucial role in microfluidic 3D cancer modeling and biosensing, enabling precise manipulation of biomaterials and living cells and offering a broad toolbox for quantifying critical biological phenomena.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Sanaz Alizadeh, Paniz Farshi, Navid Farahmandian, Zahra Aliakbar Ahovan, Ali Hashemi, Mohammad Majidi, Abdolnaser Azadbakht, Mahsa Darestanifarahani, Koushan Sineh Sepehr, Subhas C. Kundu, Mazaher Gholipourmalekabadi
Summary: A nanofibrous chitosan/poly (vinyl alcohol) (CS/PVA) mat was designed for controlled delivery of antibiotics to treat wound infections. The material showed excellent antibacterial activity against resistant strains and demonstrated good biocompatibility in vitro and in vivo.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Nanoscience & Nanotechnology
Prithwish Kola, Prasanth Kumar Bhusetty Nagesh, Pritam Kumar Roy, K. Deepak, Rui Luis Reis, Subhas C. Kundu, Mahitosh Mandal
Summary: The increasing number of breast cancer cases globally and the rising death rate indicate the inadequacy of traditional and current treatments. Nanoparticle-based therapies such as photothermal therapy, photodynamic therapy, chemodynamic therapy, and sonodynamic therapy show promise in combating breast cancer through accurate drug delivery and elimination of cancer stem cells. These innovative therapies have fewer side effects compared to standard chemotherapy and address the stability issues associated with cancer immunotherapy. This review discusses various nanotheranostic systems and smart nanoparticles, their mechanisms of action, and their relevance in the current era.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Zhenzhen Qi, Zheng Yan, Guohongfang Tan, Tianshuo Jia, Yiyu Geng, Huiyan Shao, Subhas C. C. Kundu, Shenzhou Lu
Summary: Silk fibroin microneedles have advantages in terms of biocompatibility, mechanical properties, thermal stability, and control via genetic engineering. This review summarizes the research progress, structure, properties, applications, and clinical trials of silk fibroin microneedles, and discusses the future development trends.
Article
Engineering, Biomedical
Anita Akbarzadeh Solbu, David Caballero, Spyridon Damigos, Subhas C. Kundu, Rui L. Reis, Oyvind Halaas, Aman S. Chahal, Berit L. Strand
Summary: Cell migration plays a crucial role in various biological processes, and recent advancements in materials science have allowed for the development of biomimetic in vitro assays to study cell migration. Three-dimensional hydrogels have been particularly useful in mimicking the extracellular environment and studying cell migration in a more physiologically relevant manner. This article provides an overview of the different types of hydrogels and their characteristics, as well as established methods for cell migration assays and tracking in three-dimensional environments.
MATERIALS TODAY BIO
(2023)
Article
Biotechnology & Applied Microbiology
Ilenia Iaia, Virginia Brancato, David Caballero, Rui L. Reis, Massimo Aglietta, Dario Sangiolo, Subhas C. Kundu
Summary: Little is known about the role of fibroblasts in solid tumors such as melanoma and the mechanism behind it due to the lack of relevant pre-clinical models. In this report, a melanoma-on-a-chip model was proposed to evaluate the essential role of fibroblasts in the antitumor activity of lymphocytes. The presence of fibroblasts was observed to inhibit immune effector recruitment in a 3D pre-clinical tumor model.
BIOENGINEERING-BASEL
(2023)
Article
Engineering, Multidisciplinary
Guohongfang Tan, Fujian Jiang, Tianshuo Jia, Zhenzhen Qi, Tieling Xing, Subhas C. Kundu, Shenzhou Lu
Summary: This study reports the use of polylysine-modified cationized silk fibroin (SF) to prepare microneedles (MNs) for responsive transdermal insulin delivery. The MNs were well arranged and formed an array with 0.5 mm pitch, and had a length of approximately 430 μm. They exhibited pH and glucose-responsive properties, rapidly releasing insulin as pH decreased and glucose concentration increased. In vivo experiments showed that the insulin release from cationized SF MNs was significantly lower in diabetic rats compared to normal rats. Therefore, cationized SF MNs hold promise as a new modality for diabetes treatment, replacing subcutaneous injections of insulin.
Article
Materials Science, Biomaterials
Lara Pierantoni, Virginia Brancato, Joao B. Costa, Subhas C. Kundu, Rui L. Reis, Joana Silva-Correia, Joaquim M. Oliveira
Summary: A 3D breast cancer model based on collagen protein has opened up new avenues for studying cancer progression.
Article
Multidisciplinary Sciences
Wenjing Yang, Ya Ma, Haiting Xu, Zhenhua Zhu, Jiaxue Wu, Cheng Xu, Wei Sun, Erhu Zhao, Min Wang, Rui L. Reis, Subhas C. Kundu, Xiaoxiao Shi, Bo Xiao
Summary: A fluorinated nanoparticle has been developed to improve the therapeutic outcomes of ulcerative colitis (UC). The results showed that the nanoparticles efficiently delivered drugs to the colon, promoted mucosal penetration, and had anti-oxidative and anti-inflammatory effects. In animal models, the nanoparticles significantly improved the therapeutic efficacy, reduced inflammation, and restored intestinal microbiota balance.
Article
Chemistry, Physical
Juan Du, Yue You, Rui L. Reis, Subhas C. Kundu, Jingliang Li
Summary: Gels are self-supporting soft materials widely used in various fields. The design of gels for specific applications requires fast, controllable gelation, desired micro/nano structure, and suitable rheological properties. Surfactants, although widely used in controlling material formation, are less understood in their effects on gelation. This review summarizes the studies on using different surfactants to control the formation, structure, and properties of molecular and silk fibroin gels. The mechanisms of surfactants, interfacial and non-interfacial effects, are classified and discussed. This review is expected to inspire further research on using surfactants for designing and fabricating gels with desired kinetics, structure, properties, and functionalities.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Rui R. Costa, David Caballero, Diana Soares da Costa, Romen Rodriguez-Trujillo, Subhas C. Kundu, Rui L. Reis, Iva Pashkuleva
Summary: Anisotropy plays a crucial role in the organization and healing of neural tissues, and holds great potential for developing regeneration strategies. The study demonstrates the fabrication of microfibers from charged ECM components using interfacial polyelectrolyte complexation, which can mimic the mechanical properties of neural tissues. These fibers are biocompatible and promote the formation of neural processes, enabling the development of neural cells.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Materials Science, Biomaterials
Zhanghong Gu, Suna Fan, Subhas C. Kundu, Xiang Yao, Yaopeng Zhang
Summary: Due to the innate extracellular matrix mimicking features, fibrous materials have great potential in biomedicine. A unique material platform with a non-fouling background and parallel-arranged polycaprolactone fibers is designed to eliminate interference cell adhesions and investigate the effects of fiber diameter on stem cell behaviors. The results show that the fiber diameter can significantly affect cell proliferation and osteogenesis, and the dense fiber arrangement may play a role in inducing different cell behavior trends.
REGENERATIVE BIOMATERIALS
(2023)