Article
Chemistry, Analytical
Donggu Hong, Kihyeun Kim, Eun-Jung Jo, Min-Gon Kim
Summary: CRP is a general biomarker for inflammation and infection, and the ECL-LFI is an electrochemiluminescent lateral flow immunosensor that allows full-range CRP detection with high sensitivity and a broad detection range, showing great potential for CRP detection in point-of-care diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Ali Firoozbakhtian, Morteza Hosseini, Yiran Guan, Guobao Xu
Summary: Point-of-care testing is crucial in resource-poor areas, and this study presents the development of an integrated MXene-modified SEES device for visual and high-throughput ECL immunoassays. The developed system achieved a remarkable minimum virus detection limit and showed promising potential for efficient point-of-care testing.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xianzhen Song, Lu Zhao, Nuo Zhang, Lei Liu, Xiang Ren, Hongmin Ma, Xuan Kuang, Yuyang Li, Chuannan Luo, Qin Wei
Summary: A high-efficiency biosensor based on a ternary electrochemiluminescence system was developed for procalcitonin (PCT) detection. Silver nanoclusters (Ag NCs) with stable luminescence properties were prepared using lipoic acid (LA) as the ligand, and their electrochemiluminescence in persulfate (S2O82-) was reported for the first time. The addition of alpha-Fe2O3-Pt facilitated the activation of S2O82-, enhancing the electrochemiluminescence emission of Ag NCs. The biosensor showed high sensitivity for PCT detection with a wide linear range and low detection limit.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Huaping Peng, Mingchun Lai, Huijing Wang, Zhimin Weng, Yu Yang, Zhongnan Huang, Weiming Sun, Juewen Liu, Wei Chen
Summary: In this study, an energy level engineering strategy was proposed to regulate the electrochemiluminescence (ECL) performance using ligand-protected gold nanoclusters (AuNCs) as luminophores and N,N-diisopropylethylamine (DIPEA) as a coreactant. The energy level matching between the AuNCs and DIPEA effectively promoted electron transfer reactions, improving excitation efficiency and reducing trigger potential. The narrow bandgap of the AuNCs further enhanced emission efficiency. The developed theory was successfully applied to design a highly stable near-infrared ECL system with low trigger potential.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Brady R. Layman, Jeffrey E. Dick
Summary: Multiphase chemical systems, with their unique environment and properties, require the development of new tools for a detailed understanding. In this study, electrogenerated chemiluminescence (ECL) is used to precisely and comprehensively elucidate phase boundaries in a multiphase system. The results reveal unexpected lack of electronic communication in solvent inclusions, which has implications for energy storage and conversion devices and wearable/implantable sensors.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Analytical
Pinlong Zhao, Wenxin Zhu, Min Zheng, Jiandong Feng
Summary: A deep enhanced ECL microscopy (DEECL) method is proposed, which utilizes artificial neural networks to reconstruct electrochemiluminescence images with millisecond exposure times. DEECL achieves similar quality as high-quality electrochemiluminescence images with second-long exposure time. The DEECL method improves imaging efficiency by 1 to 2 orders than usual and achieves 85% accuracy in cell classification application.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yilei Lu, Xiujun Rong, Peng Wu, Jian Shou, Long Chen, Fang Luo, Cuiying Lin, Jian Wang, Bin Qiu, Zhenyuo Lin
Summary: By utilizing the discrepancy of electrostatic interaction between negatively charged signal molecules and long and short DNA strands on the modified electrode surface, an electrochemiluminescence (ECL) biosensor for HPV 16 DNA detection has been developed. This innovative methodology offers sensitive detection of HPV concentration by controlling electrostatic interaction and steric hindrance.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Joo Yeon Kim, Sanghoon Cheon, Dae Kyom Kim, Sooji Nam, Jisu Han, Chi-Sun Hwang, Yuanzhe Piao, Jeong-Ik Lee
Summary: By changing the solvent systems, a significantly enhanced brightness of rubrene-based electrochemiluminescence cells can be achieved.
Article
Chemistry, Analytical
Xiang-Lan Mao, Qiu-Xia Luo, Yuan-Jun Cai, Xin Liu, Qiao-Qiao Jiang, Cheng-Rong Zhang, Ru-Ping Liang, Jian-Ding Qiu
Summary: The isomerism of a covalent organic framework (COF) greatly affects its electrochemiluminescence (ECL) performance. In this study, two isomeric COFs, TFPB-BD(OMe)(2)-H and TAPB-BD(OMe)(2)-H, were synthesized based on different imine linkages and conversion to the quinoline structure. Despite having the same composition and similar structures, the two COFs exhibited significant differences in photoelectrochemical and ECL fields. TFPB-BD(OMe)(2)-H showed a superior ECL emission compared to TAPB-BD(OMe)(2)-H due to stronger polar interaction and enhanced electron interactions.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jia-Li Liu, Jia-Qi Zhang, Ying Zhou, Dong-Rong Xiao, Ying Zhuo, Ya-Qin Chai, Ruo Yuan
Summary: By altering molecular structures and synthesizing nanocrystals with ordered molecular structures, the efficiency of ECL emission can be enhanced. Among them, TPB NCs exhibit the highest ECL efficiency due to the unique J-aggregates and restricted molecular rotation, showing great potential in various applications.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Yujie Liu, Hongding Zhang, Binxiao Li, Jianwei Liu, Dechen Jiang, Baohong Liu, Neso Sojic
Summary: This study successfully imaged single biomolecules using RuDSNs/AuNPs as ECL nanoemitters, demonstrating the visualization of single protein molecules and single membrane proteins in cells, overcoming the challenges in ultrasensitive ECL analysis and providing valuable information for cellular biology research.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Jingjing Zhang, Lin Hao, Jie Chao, Lianhui Wang, Shao Su
Summary: In this study, an ECL imaging technique for single cell membrane protein based on Co3O4 nanozyme catalytic enhancement strategy was developed. The highly efficient catalytic performance of Co3O4 nanozymes allows efficient decomposition of H2O2 into reactive oxygen radicals, leading to enhanced reaction with the luminescence reagent L012 and stronger ECL emission. The optimized design of Co3O4 nanozymes coupled with anti-CEA enables specific recognition of overexpressed CEA on the MCF-7 cell membrane, enabling rapid ECL imaging of single cell membrane protein.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Hui Zhu, Dechen Jiang, Jun-Jie Zhu
Summary: By mapping the electrocatalytic activity of a single graphene sheet with nanometer resolution using electrochemiluminescence (ECL) microscopy, this study effectively avoids the problem of overlapping ECL emissions from adjacent regions and achieves nanometer spatial resolution for the first time. The varied adsorption of hydrogen peroxide on the graphene surface leads to unsynchronized ECL emission, allowing the ECL intensity of each pixel to reflect the electrocatalytic features of the graphene surface with a resolution of 400 nm.
Article
Chemistry, Analytical
Zhen Luo, Weiqing Xu, Zhichao Wu, Lei Jiao, Xin Luo, Mengzhen Xi, Rina Su, Liuyong Hu, Wenling Gu, Chengzhou Zhu
Summary: The Ru(bpy)(3)(2+)-tripropylamine ECL system is widely used in commercial bioanalysis, but the presence of amines in the biological environment causes unwanted anodic interference signals. To overcome this, a cathodic Ru(bpy)(3)(2+) ECL system is proposed, which utilizes the iron-nitrogen-carbon catalyst to activate the ECL signal. This innovative system shows great potential for practical applications, demonstrated by the successful development of an ECL biosensor for detecting alkaline phosphatase activity.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Gerardo Salinas, S. Mohsen Beladi-Mousavi, Liubov Gerasimova, Laurent Bouffier, Alexander Kuhn
Summary: Bipolar electrochemistry (BE) is a wireless electrochemical technique that utilizes an array of light-emitting diodes with different lengths to image the transient electrochemical activity of different redox species. The intensity of light emission allows differentiation of redox systems and evaluation of their diffusion behavior.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Yaqi Liu, Fengyi Wang, Shuo Ge, Lu Zhang, Zuhao Zhang, Yunqing Liu, Yan Zhang, Shenguang Ge, Jinghua Yu
Summary: A strand displacement amplification (SDA)-assisted CRISPR/Cas12a electrochemiluminescence (ECL) biosensor was developed for ultrasensitive detection of Staphylococcus aureus (Sa)-16S rDNA. The biosensor utilized a porphyrinic Zr metal-organic framework (MOF) as a coreactant accelerator to enhance the ECL emission signal. The presence of Sa-16S rDNA triggered the SDA reaction, generating single-stranded DNA products (SP) that activated the CRISPR/Cas12a trans-cleavage and effectively extinguished the ECL signals. The ECL platform exhibited high sensitivity and specificity, making it suitable for clinical diagnosis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Qingxin Zhang, Manru Zhang, Zhiqiang Guo, Jingjing Li, Zhixue Zhu, Yu Wang, Su Liu, Jiadong Huang, Jinghua Yu
Summary: In this study, a low-background electrochemical biosensor based on a DNA tetrahedron-besieged primer and a DNAzyme-activated programmatic rolling circle amplification (RCA) was proposed for OTA detection in wine samples. The low-background detection was achieved by using the besieged primer and the target OTA-mediated DNAzyme activation initiated the programmatic RCA. The integration of the catalytic property of silver nanoclusters (AgNCs) with the electrochemical assay provided high sensitivity for OTA detection. This novel design showed great potential in food safety and environmental detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Xu Li, Jiali Huang, Jiayu Ding, Mingzhen Xiu, Kang Huang, Kang Cui, Jing Zhang, Shiji Hao, Yan Zhang, Jinghua Yu, Yizhong Huang
Summary: In this study, a dual-mode lab-on-paper device was constructed using BiVO4/FeOOH nanocomposites as an efficient photoelectrochemical (PEC)/colorimetric signal reporter for sensitive detection of carcinoembryonic antigen (CEA). The nanocomposites with an FeOOH needle-like structure displayed enhanced PEC response activity, enabling quantitative detection of CEA with the PEC signal readout mode. Additionally, a colorimetric signal readout system was achieved through a smart folding technique, providing a dual-mode signal readout for the lab-on-paper device.
Article
Chemistry, Analytical
Qian Wang, Liang Lv, Wenhao Chi, Yujiao Bai, Wenqing Gao, Peihua Zhu, Jinghua Yu
Summary: A new porphyrin-based porous covalent organic framework (TAP-COF) with high surface areas and efficient electron transport was synthesized. The TAP-COF exhibited excellent peroxidase-like catalytic activity and was able to catalyze the oxidation of a substrate by hydrogen peroxide. Simple and rapid colorimetric methods for in situ hydrogen peroxide detection were developed using this TAP-COF nanozyme, which has potential applications in point-of-care testing, cell biology, and clinical diagnosis.
Article
Chemistry, Analytical
Huihui Shi, Yanfei Che, Yumeng Rong, Jiajun Wang, Yanhu Wang, Jinghua Yu, Yan Zhang
Summary: An integrated dual-signal bioassay was developed for ultrasensitive detection of thrombin. This method combined visualization with photoelectrochemical technique to achieve the detection. The bioassay demonstrated wide linear ranges, high selectivity, stability, and reproducibility under optimized conditions. Additionally, the proposed visual/photoelectrochemical transduction mechanism platform offered a lower background signal and more reliable detection results, providing an effective way for detecting other proteins and nucleic acids.
Article
Chemistry, Analytical
Wenli Xiu, Peini Zhao, Yujie Pan, Xiaoru Wang, Lina Zhang, Shenguang Ge, Jinghua Yu
Summary: A surface-enhanced Raman scattering (SERS) sensor was developed for rapid on-site determination of ethephon in food, based on the folding and assembly characteristics of paper fibers, the skeleton controllability of metal-organic framework materials (MOFs), and the morphology designability of plasmonic noble metal materials. The sensor used HKUST-1(Cu) assembled onto a carbon-treated chromatographic paper matrix to overcome poor affinity of SERS substrate for target molecules. With the coupling of biomimetic antibodies and a multi-stage progressive enhancement structure, the sensor achieved high specificity and sensitivity for ethephon detection with a linear range of 10-3 to 10 mg kg-1 and a low detection limit of around 1.39 x 10-4 mg kg-1.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Li Shan, Yuanyuan Chen, Xiaoran Tan, Shenguang Ge, Lina Zhang, Lin Li, Jinghua Yu, Li Li
Summary: A newly designed two-in-one tetrahedral DNA (TDN) nanostructure with an antifouling surface and backbone-rigidified interfacial tracks was developed for highly sensitive and selective detection of miRNA-182-5p. The TDN-modified sensing interfaces demonstrated incomparable antifouling ability against protein samples and interfering miRNAs due to the strong hydrophilic capacity and special molecular conformations. The designed TDN-modified paper-based photoelectro-chemical sensor successfully quantified miRNA-182-5p with a low detection limit of 0.09 fM and high specificity in real samples, showing great promise for bioanalysis and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Shanshan Li, Haihan Yu, Yuwen Wang, Shuai Wang, Lina Zhang, Peihua Zhu, Chaomin Gao, Jinghua Yu
Summary: A stable organic-inorganic Z-scheme heterostructure was constructed by covalently connecting COF-366-Fe and CeO2 through Schiff base reaction, which effectively integrated their respective properties and improved the separation and transfer of photoexcited carriers. The COF-366-Fe@CeO2 exhibited superior photocatalytic CO yield compared to COF-366-Fe and CeO2, indicating its potential as a highly-effective COF-based photocatalyst for artificial photosynthesis.
APPLIED SURFACE SCIENCE
(2023)
Article
Biophysics
Lin Li, Hui Tian, Guofu Wang, Suyue Ren, Tinglei Ma, Yixiang Wang, Shenguang Ge, Yan Zhang, Jinghua Yu
Summary: Ready-to-use in vitro diagnosis is crucial for cervical cancer prevention and treatment. A paper-film-based differential lateral flow biosensor is proposed, allowing simultaneous detection of two high-risk HPV types. The biosensor utilizes a CuCo2S4/ZnIn2S4 heterostructure on a paper-film chip and introduces surface sulfur vacancy for enhanced photoexcitation. Through sequential solution transfer, the biosensor activates the self-assembly of DNA and the cleavage activity of CRISPR-Cas12a, resulting in the release of polydopamine-coated gold nanoparticles. This intelligent biosensor achieves low limit-of-detection for HPV-18 and HPV-16 and provides a promising approach for home medical intelligent diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Hao Cheng, Yuying Wang, Yue Wang, Lei Ge, Xiaojuan Liu, Feng Li
Summary: A simple colorimetric sensor based on the peroxidase-like catalytic activity of Ni/Co LDHs was developed for sensitive detection of AChE. The high peroxidase-like activity of Ni/Co LDHs enables rapid catalysis of OPD to produce yellow oxOPD in the presence of H2O2. The presence of AChE effectively inhibits the peroxidase-like activity of Ni/Co LDHs, leading to the failure of OPD oxidation.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Limin Yang, Liuying Chen, Zhiliang Feng, Haiyan Shi, Baokun Wang, Wenjie Liu, Xiaojuan Liu, Ting Hou, Lei Ge, Feng Li
Summary: A laser-induced Fe3O4 nanoparticle-modified three-dimensional macroporous graphene is fabricated for the first time via a facile and in situ laser-scanning method under ambient conditions, showing excellent catalytic-photothermal synergetic bactericidal ability under a low dose of H2O2 (0.1 mM) and short irradiation time (5.0 min).
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Wenhao Wang, Lu Zhang, Yanli Kang, Xiaodong Yang, Shenguang Ge, Feng Yu
Summary: A TiO2-CoFe2O4 heterostructure was successfully prepared using a hydrothermal method for detecting toluene gas. The heterojunction exhibited a shorter bandgap and inhibited electron-hole pair recombination, leading to improved gas sensitivity. The response value of the heterojunction was significantly higher than that of pure TiO2 and CoFe2O4, especially under UV irradiation. Density functional theory was also applied in this study to explain the improvement in gas sensitivity through material energy band and adsorption energy calculations. This work provides valuable insights for the preparation of high-efficiency and high-sensitivity gas sensors.
DALTON TRANSACTIONS
(2023)
