Article
Biochemistry & Molecular Biology
Samantha Baldassarri, Daniela Benati, Federica D'Alessio, Clarissa Patrizi, Eleonora Cattin, Michela Gentile, Angelo Raggioli, Alessandra Recchia
Summary: This study demonstrates the use of SB transposon to engineer HEK293 cells for improved production of chimpanzee Adenovector. By employing genetic engineering and CRISPR/Cas9 technology, the essential genes for chimpanzee Adenoviral DNA replication were successfully integrated into HEK293 cells, leading to significantly enhanced vector production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Sarah C. Saunderson, S. M. Ali Hosseini-Rad, Alexander D. McLellan
Summary: Tetracycline-inducible systems are widely used control elements for mammalian gene expression. The G72V mutation in the rtTA-M2 tetracycline activator reduces basal level expression and increases fold-induction. Sensitivity enhancing mutations compensate for the loss of responsiveness caused by the G72V mutation without compromising promoter tightness.
Article
Biochemistry & Molecular Biology
Aiping Zhang, Lijian Wang, Josh Haipeng Lei, Zhengqiang Miao, Monica Vishnu Valecha, Peng Hu, Kai Miao, Chu-Xia Deng
Summary: SB Digestor is a new computational algorithm that overcomes biological heterogeneity and accurately detects and defines cancer driver genes, providing more potential candidates for study.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Review
Oncology
Haruna Takeda, Nancy A. Jenkins, Neal G. Copeland
Summary: Cancer genome sequencing studies have identified driver genes for various cancers, but identifying these genes solely from genetic data remains challenging. In vivo forward genetic screens using SB transposon mutagenesis in mice provide a powerful tool for identifying potential cancer driver genes. Comparing driver genes identified in human and mouse tumors can enhance confidence in identifying cancer driver genes.
Article
Genetics & Heredity
Yiting Zhou, Guangwei Ma, Jiawen Yang, Zenghong Gao, Yabin Guo
Summary: Recent study showed that Sleeping Beauty transposon can integrate into non-TA sites, mainly through aberrant integrations. The consensus sequence of the non-TA target sites corresponds to the transposon end sequence.
FRONTIERS IN GENETICS
(2021)
Review
Virology
Matthias T. Ochmann, Zoltan Ivics
Summary: The Sleeping Beauty transposon system has been widely used as a genetic engineering tool, with extensive studies elucidating the molecular mechanisms and actions during SB transposition process. Structural data on the SB transposase provide a direct insight into the enzyme's functionality, leading to the development of novel SB variants for advanced genetic engineering possibilities. However, many aspects of transposition process remain poorly understood, necessitating further research to explore the structure-function relationships of SB transposition for the development of new vector systems.
Article
Medicine, Research & Experimental
Kun Ma, Wenzhe Li, Guang Zhu, Shibo Sun, Hao Chi, Yalin Yin, He Diao, Xiao-Jin Xing, Zhaoming Guo, Li Wang, Weiping Xu, Changhao Cui, Jianqiang Xu
Summary: The CRISPR/Cas9 gene was cloned into a non-viral safe vector Sleeping-Beauty (SB) transposon to construct a safer vector. In addition, PDA/DEX-PEI@HA (PDPH) nanoparticles were developed to facilitate precise CRISPR/Cas9 targeted delivery, showing high transfection efficiency and minimal cytotoxicity. The study highlights the potential of PDA/DEX-PEI25k@HA/pT25pCas9 (PDPH25k/pT(25K)pCas9) as a non-viral gene vector for CRISPR/Cas9 delivery in clinical medication.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Eliot Y. Zhu, Jacob L. Schillo, Sarina D. Murray, Jesse D. Riordan, Adam J. Dupuy
Summary: Combined BRAF and MEK inhibition is an effective treatment for BRAF-mutant cutaneous melanoma, but drug resistance remains a challenge. This study utilized the Sleeping Beauty transposon system to investigate the mechanisms of drug resistance in melanoma. The findings demonstrated the importance of considering melanoma heterogeneity and identified a potential target for overcoming drug resistance.
Article
Oncology
Michiko Kodama, Hiroko Shimura, Jean C. Tien, Justin Y. Newberg, Takahiro Kodama, Zhubo Wei, Roberto Rangel, Kosuke Yoshihara, Airi Kuruma, Aya Nakae, Kae Hashimoto, Kenjiro Sawada, Tadashi Kimura, Nancy A. Jenkins, Neal G. Copeland
Summary: This study identified 19 candidate cancer genes driving uterine leiomyosarcoma (ULMS) through an in vivo Sleeping Beauty transposon mutagenesis screen. ZNF217 and NRDC were highlighted as potential therapeutic targets for ULMS treatment.
Article
Optics
Yutian Wang, Chenxi Li, Fanglin Chen, Hengwen Lan, Songnian Fu, Mariusz Klimczak, Ryszard Buczynski, Xiahui Tang, Ming Tang, Luming Zhao
Summary: The study of soliton-based fiber optic communication and signal processing techniques has been hindered by hardware and digital signal processing limitations. However, the recent introduction of nonlinear Fourier transform (NFT) has provided a powerful mathematical framework for analyzing and understanding soliton behavior in optical fibers, leading to new developments in the field.
OPTICS COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Nicolas Sandoval-Villegas, Wasifa Nurieva, Maximilian Amberger, Zoltan Ivics
Summary: Transposons are mobile genetic elements that have been used as experimental tools to introduce foreign DNA sequences into target cells in a regulated and highly efficient manner. They are supplied as naked nucleic acids or recombinant proteins, making their use simple, safe, and economically competitive. Transposons enable various avenues for genome manipulations in vertebrates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Yun Haeng Lee, Ji Yun Park, Eun Seon Song, Haneur Lee, Myeong Uk Kuk, Junghyun Joo, Hyungmin Roh, Joon Tae Park
Summary: This study proposes a new strategy to increase the efficiency and stability of protein production by modifying the traditional SB transposon vector. Adding a pair of inverted terminal repeats (ITRs) next to existing ITRs significantly improves transgene integration efficiency, resulting in high-yield and sustained protein production. Moreover, double-ITRs show a more favorable response to DNA methylation inhibitors, suggesting their potential in increasing protein productivity.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Larissa Mattern, Katrin Otten, Csaba Miskey, Matthias Fuest, Zsuzsanna Izsvak, Zoltan Ivics, Peter Walter, Gabriele Thumann, Sandra Johnen
Summary: This study presents a potential treatment option for dry AMD by transferring the BDNF gene into RPE cells. The expression of BDNF in the transfected cells leads to reduced apoptosis rates and stimulated neurite outgrowth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Juane Lu, Tao Wu, Biao Zhang, Suke Liu, Wenjun Song, Jianjun Qiao, Haihua Ruan
Summary: This article summarizes the types of nuclear localization signals, focuses on recently reported proteins containing such signals, and briefly outlines mechanisms for nuclear entry that do not rely on nuclear localization signals.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Biochemistry & Molecular Biology
Du-Hyong Cho, Yun-Jin Hwang, Jin Hee Park, Jee Young Lee, Jung-Hyun Park, Inho Jo
Summary: This study developed an organelle-specific, peptide-based NO delivery material that targets the nucleus. The delivery concept can be extended to cells other than stem cells to explore the effects of NO release in the nucleus. Furthermore, NO can be delivered to other organelles such as mitochondria and lysosomes through conjugation with specific targeting signals.
BIOORGANIC CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Qing Bao, Ying Zhang, Xiangyu Liu, Tao Yang, Hui Yue, Mingying Yang, Chuanbin Mao
Summary: Due to the complexity and heterogeneity of cancer, there is a high demand for clear and accurate tumor imaging and image-guided multimodal synergistic therapy. In this work, bismuth-based mesoporous silica-coated nanoparticles (BMSNs) were successfully fabricated with a thin silica shell and modified with an MCF-7 breast cancer-targeting peptide. The resulting nanoparticles showed enhanced drug loading and aggregation in the tumor site, enabling enhanced computed tomography imaging and synergistic chemo-photothermal therapy.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Materials Science, Biomaterials
Yingfan Chen, Xiangyu Liu, Mingying Yang, Weilian Sun, Chuanbin Mao
Summary: Peripheral nerve injury (PNI) leads to persistent abnormalities in sensory function. The current treatment methods for large nerve defects are not effective. In this study, a genetically engineered virus nanofiber was developed as an artificial nerve fiber graft, which promoted nerve regeneration and neurogenesis in a rat model. This phage-based biomaterial shows potential as a substitute for autologous nerve grafts (ANGs) in treating PNI.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Nutrition & Dietetics
Siwei Chen, Dan Dai, Jian Zheng, Haoyu Kang, Dongdong Wang, Xinyu Zheng, Xiaobo Gu, Jiali Mo, Zhuohui Luo
Summary: This study proposes a deep-learning model based on a spatial attention mechanism and SE-network structure to grade walnut kernels. The ResNet152V2-SA-SE model exhibits the best performance with a maximum test set accuracy of 92.2%.
FRONTIERS IN NUTRITION
(2023)
Article
Chemistry, Multidisciplinary
Mengyi Qiu, Xiaorong Zhu, Shuowen Bo, Kai Cheng, Nihan He, Kaizhi Gu, Dezhong Song, Chen Chen, Xiaoxiao Wei, Dongdong Wang, Yingying Liu, Shuang Li, Xiaojin Tu, Yafei Li, Qinghua Liu, Conggang Li, Shuangyin Wang
Summary: A multihole structure was shown to preserve the Cu+ component of Cu2O spheres under electrochemical reduction conditions, promoting the asymmetric coupling process of *CO and *NO and boosting urea production.
Article
Oceanography
Gongcheng Zhang, Ying Chen, Dongdong Wang, Zengxue Li
Summary: The sea area in China is an important region for oil and gas exploration, primarily consisting of coal-type hydrocarbons. Coal-bearing source rocks have become a crucial focus in exploration efforts. Comprehensive geological research indicates that the region has undergone numerous tectonic movements, resulting in the formation of 26 Cenozoic sedimentary basins of various types. Recent findings reveal the development of a significant C-shaped coal-bearing basin group in 14 continental margin basins. Analysis based on paleoclimate, paleobotany, paleogeography, and paleostructure suggests the presence of five coal-forming periods, with coal seams mainly developed in three Cenozoic environments. Despite their thinness, multiple layers, and poor stability, the coal-bearing series source rocks in the sea area exhibit a wide distribution range, notable thickness, and significant reserves, providing a material basis for the formation of abundant coal-type oil and gas resources.
ACTA OCEANOLOGICA SINICA
(2023)
Article
Chemistry, Multidisciplinary
Xiaoxiao Wei, Yingying Liu, Xiaorong Zhu, Shuowen Bo, Lei Xiao, Chen Chen, Ta Thi Thuy Nga, Yuanqing He, Mengyi Qiu, Chao Xie, Dongdong Wang, Qinghua Liu, Fan Dong, Chung-Li Dong, Xian-Zhu Fu, Shuangyin Wang
Summary: Reported herein is a method for synthesizing urea using copper single atoms decorated on a CeO2 support (Cu-1-CeO2) as the catalyst, which exhibits an average urea yield rate of 52.84 mmol h(-1) g(cat.)(-1) at -1.6 V versus reversible hydrogen electrode. Operando X-ray absorption spectra demonstrate the reconstitution of copper single atoms (Cu-1) to clusters (Cu-4) during electrolysis. Favorable C-N coupling reactions and urea formation on Cu-4 are validated using operando synchrotron-radiation Fourier transform infrared spectroscopy and theoretical calculations. Dynamic and reversible transformations of clusters to single-atom configurations occur when the applied potential is switched to an open-circuit potential, endowing the catalyst with superior structural and electrochemical stabilities.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Dongdong Wang, Xiaorong Zhu, Xiaojin Tu, Xiaoran Zhang, Chen Chen, Xiaoxiao Wei, Yafei Li, Shuangyin Wang
Summary: An atomic copper-iron dual-site electrocatalyst anchored on nitrogen-doped carbon has been developed for electrocatalytic reduction of nitric oxide to ammonia. The CuFe DS/NC catalyst exhibited significantly better performance compared to other single-atom catalysts, with a high Faraday efficiency of 90% and a yield rate of 112.52 μmol cm(-2) h(-1) at -0.6 V versus RHE. Furthermore, the CuFe DS/NC catalyst showed promising results in a proof-of-concept Zn-NO battery. Theoretical calculations suggested that the bimetallic sites played a crucial role in promoting the electrocatalytic reaction.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yandong Wu, Wei Chen, Yimin Jiang, Yanzhi Xu, Bo Zhou, Leitao Xu, Chao Xie, Ming Yang, Mengyi Qiu, Dongdong Wang, Qie Liu, Qinghua Liu, Shuangyin Wang, Yuqin Zou
Summary: The synthesis of cyclohexanone oxime using the cyclohexanone-hydroxylamine process is widely used in the caprolactam industry. However, this process has drawbacks such as harsh reaction conditions and the potential danger of explosive hydroxylamine. This study presents a direct electrosynthesis of cyclohexanone oxime using nitrogen oxides and cyclohexanone, eliminating the need for hydroxylamine and demonstrating a green production of caprolactam. The use of Fe electrocatalysts results in a high production rate and almost 100% yield of cyclohexanone oxime in a flow cell. This study provides a theoretical basis for electrocatalyst design and offers the potential to upgrade the caprolactam industry towards safety and sustainability.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Engineering, Biomedical
Chen Zhuang, Guanglin Zhu, Yingjun Wang, Lin Wang, Xuetao Shi, Chuanbin Mao
Summary: Insoluble metal bisphosphonates (BPs) are developed through crystallization on a solid calcium hydroxyapatite (HA)-based substrate, presenting high purity, regular morphologies and excellent biodegradability. These CaBPs can induce osteogenic differentiation of adipose-derived mesenchymal stem cells in vitro and stimulate bone formation effectively in a femur defect rabbit model, showing great potential as biomaterials for tissue regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Chuanbin Mao, Songyuan Yao, Lin Wang, Penghe Qiu
Summary: Considerable progress has been made on the synthetic chemistry and antibacterial application of tricobalt tetroxide (Co3O4) nanomaterials. However, the current approaches to synthesizing Co3O4 nanomaterials are complex. In this study, a one-pot strategy was developed to synthesize Co3O4 nanowires with antibacterial activity using engineered bacterial flagella as a biotemplate. The flagella displaying negatively charged peptides effectively induced the nucleation of Co3O4 nanoparticles on their surface to form polycrystalline nanowires. The E20-flagella showed higher effectiveness than the E10-flagella.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Minghao Wei, Yiyang Lin, Yan Qiao
Summary: Artificial counterparts of living cells that bridge the gap between non-living and living materials have been developed. Cell-sized colloidosomes have been utilized as biomimetic cell models due to their robustness and versatility. This review highlights recent advances in the design of cell-like colloidosomes, which mimic advanced cellular behaviors and possess hierarchical structures and functional units. Potential applications in soft robotics, microreactors, and drug delivery are envisioned.
Article
Public, Environmental & Occupational Health
Xiao Gu, Dong D. Wang, Laura Sampson, Junaidah B. Barnett, Eric B. Rimm, Meir J. Stampfer, Luc Djousse, Bernard Rosner, Walter C. Willett
Summary: This study evaluated the validity and reproducibility of a semiquantitative food frequency questionnaire (FFQ) and found that it performed well in measuring most foods and food groups.
AMERICAN JOURNAL OF EPIDEMIOLOGY
(2023)
Article
Chemistry, Analytical
Yueyi Sun, Xuewei Qu, Penghe Qiu, Chuanbin Mao
Summary: Molecular beacons (MBs) are DNA-based probes used for detecting DNA or RNA fragments. A nanoparticle-based MB (NPMB) utilizing upconversion nanoparticles (UCNPs) as fluorophores is proposed to avoid background autofluorescence and enable the detection of small RNA from complex clinical samples. The NPMB demonstrates ultrasensitive detection of small nucleic acid biomarkers in a range of clinical samples.
Article
Chemistry, Multidisciplinary
Yuping Chen, Ruyin Lyu, Jie Wang, Qichao Cheng, Yanfang Yu, Shuxu Yang, Chuanbin Mao, Mingying Yang
Summary: This study presents a novel nanoplatform constructed using zeolitic imidazolate framework-8 (ZIF-8) mediated by silk fibroin (SF), modified with a newly discovered MCF-7 breast tumor-targeting peptide (AR peptide). The nanoplatform loaded with indocyanine green (ICG) and doxorubicin (DOX) enables targeted and controlled drug delivery for multimodal therapy, including photodynamic therapy (PDT) and photothermal therapy (PTT), as well as chemotherapy. The results demonstrate the promising potential of the nanoplatform for targeted and effective treatment of breast tumors with minimal side effects.
Article
Biotechnology & Applied Microbiology
Xin Wang, Yongtao Zhang, Dongdong Wang, Ning Su, Li Yang, Hao Fu, Jiachan Zhang, Meng Li, Changtao Wang
Summary: Aureobasidium pullulans lysate has anti-aging and anti-inflammatory effects, reduces the loss of extracellular matrix components, scavenges excess reactive oxygen species, and regulates oxidative stress homeostasis.
BIORESOURCES AND BIOPROCESSING
(2023)