Article
Medicine, Research & Experimental
Biao Wu, Jie Shang, Shiyuan Lin, Ning Jiang, Baizhou Xing, Rong Peng, Xianghe Xu, Huading Lu
Summary: Increased bone resorption caused by excessive osteoclasts is the main cause of osteoporosis. The expression of RILP, a protein, is induced by receptor activator of NF-KB ligand, and its inhibition decreases the number and function of osteoclasts. Inhibition of RILP also reduces preosteoclast migration and bone resorption. The study suggests that RILP plays a crucial role in osteoclast formation and bone resorption, and could be a therapeutic target for bone diseases caused by excessive osteoclasts.
LABORATORY INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Hye Jung Ihn, Jiwon Lim, Kiryeong Kim, Sang-Hyeon Nam, Soomin Lim, Su Jeong Lee, Jong-Sup Bae, Tae Hoon Kim, Jung-Eun Kim, Moon-Chang Baek, Yong Chul Bae, Eui Kyun Park
Summary: Ciclopirox effectively inhibits osteoclast differentiation and bone resorption both in vitro and in vivo, making it a potential candidate for preventing osteoporosis and other osteoclast-related bone diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Chenhui Cai, Wenhui Hu, Ying Zhang, Xu Hu, Sizhen Yang, Hao Qiu, Rujie Wang, Min Ma, Yiyun Qiu, Tongwei Chu
Summary: BCI treatment attenuates osteoclast differentiation and alleviates osteoporosis by inhibiting STAT3 and NF-kappa B signaling pathways, suggesting a potential effective approach for treating osteoporosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Yin Cheng, Haixia Liu, Jing Li, Yujie Ma, Changheng Song, Yuhan Wang, Pei Li, Yanjing Chen, Zhiguo Zhang
Summary: Monascin was found to inhibit osteoclastogenesis in RANKL-induced RAW264.7 cells by suppressing MAPKs signaling pathways.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell Biology
Kazuki Inoue, Courtney Ng, Yuhan Xia, Baohong Zhao
Summary: The translation discusses the role of osteoclasts in bone development and remodeling, as well as the significance of epigenetic regulation and microRNAs in gene expression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Shumin Wang, Eri O. Maruyama, John Martinez, Justin Lopes, Trunee Hsu, Wencheng Wu, Wei Hsu, Takamitsu Maruyama, Ritu Trivedi
Summary: miRNA is an ideal master regulator for organogenesis due to its ability to simultaneously modulate a set of genes for lineage-specific development. However, the lack of obvious phenotypic defects in most miRNA deletions may be attributed to functional redundancy. Our study identifies MIR27a as a specific miRNA essential for bone homeostasis, as its loss causes severe osteoporosis in mice. MIR27a affects bone resorption by osteoclasts and not bone formation by osteoblasts, and its effects are mediated through the regulation of Squstm1/p62.
Review
Biochemistry & Molecular Biology
Xin Zhao, Suryaji Patil, Fang Xu, Xiao Lin, Airong Qian
Summary: The article focuses on the importance of osteoclasts in bone metabolism and their relationship with osteoporosis, while also introducing some drug therapies targeting these molecules.
Article
Immunology
Shicheng Huo, Xuesong Liu, Shutao Zhang, Zhuocheng Lyu, Jue Zhang, You Wang, Bin'en Nie, Bing Yue
Summary: Our study shows that A-485 has a protective effect in osteoporosis and can inhibit RANKL-induced osteoclast differentiation while reducing the expression of genes associated with osteoporosis. Additionally, A-485 also attenuated OVX-induced bone loss in a mouse model by inhibiting MAPK pathway phosphorylation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Review
Cell Biology
Zhong-Hua Chen, Jun-Jie Wu, Dan-Yang Guo, Yue-Yue Li, Meng-Nan Chen, Zhen-Yu Zhang, Zheng-Dong Yuan, Kai-Wen Zhang, Wei-Wei Chen, Fan Tian, Jun-Xing Ye, Xia Li, Feng-Lai Yuan
Summary: With aging, bone tissue undergoes significant changes, leading to senile osteoporosis, a common bone disease characterized by reduced bone mass and increased fracture risk. These changes are primarily due to the disorder in the formation and resorption of bone cells, osteoblasts and osteoclasts. Osteoclasts, as bone-resorbing cells, have unique cytoskeletal structures called podosomes, which play a vital role in the degradation of mineralized bone matrix. This review summarizes recent advancements in understanding the architecture and functions of podosomes and discusses their regulatory pathways, providing insights for future research and potential therapeutic interventions for inhibiting bone resorption.
AGEING RESEARCH REVIEWS
(2023)
Article
Medicine, Research & Experimental
Seong Cheol Kim, Hyeon Jeong Kim, Gi Eun Park, Ramesh Prasad Pandey, Jisun Lee, Jae Kyung Sohng, Yong Il Park
Summary: This study demonstrated that Tri inhibits osteoclastic cell differentiation and bone resorption by down-regulating the expression of osteoclastogenic marker genes and signalling proteins, bone resorptive proteins, and by augmenting serum OPG/RANKL ratio, suggesting that Tri can be a novel anti-osteoporotic compound for treating senile and postmenopausal OP.
Article
Biochemistry & Molecular Biology
Gyeong Do Park, Yoon-Hee Cheon, So Young Eun, Chang Hoon Lee, Myeung Su Lee, Ju-Young Kim, Hae Joong Cho
Summary: Osteoporosis is a systemic metabolic bone disorder characterized by excessive bone resorption by osteoclasts. Beta-boswellic acid (β-BA) derived from Boswellia serrata shows anti-resorptive effects by inhibiting osteoclast differentiation and bone resorption, suggesting its potential as a therapeutic candidate for bone diseases like osteoporosis.
Review
Chemistry, Medicinal
Song Zhang, Zhujun Cheng, Yanan Wang, Tianyu Han
Summary: The progress of miRNA drugs in clinical trials is slow due to the characteristic of targeting multiple genes, leading to inevitable adverse effects.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Perry C. Caviness, Dongzheng Gai, Oxana P. Lazarenko, Michael L. Blackburn, Fenghuang Zhan, Jin-Ran Chen
Summary: Osteoclasts derived from hematopoietic stem cells play a crucial role in bone resorption. Identifying new molecules that can epigenetically regulate osteoclastogenesis is essential for the development of novel treatments for bone deterioration disorders like osteoporosis. The protein Ezh2 has been found to be associated with the epigenetic regulation of various cellular processes, but its role in bone cell development is still not fully understood.
Review
Immunology
Tatjana Bordukalo-Niksic, Vera Kufner, Slobodan Vukicevic
Summary: Bone remodeling in the adult skeleton involves a continuous process of old and damaged bone removal by osteoclasts and subsequent new bone formation by osteoblasts. BMPs play a crucial role in maintaining healthy bone, affecting both bone formation and resorption, and may be involved in pathological processes related to inflammatory disorders affecting bones and joints.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Guochun Zhu, Wei Chen, Chen-Yi Tang, Abigail McVicar, Diep Edwards, Jinwen Wang, Matthew McConnell, Shuying Yang, Yang Li, Zhijie Chang, Yi-Ping Li
Summary: Cathepsin K plays a role in epigenetic regulation in osteoclasts, regulating gene expression and maintaining bone homeostasis through protein proteolysis. This finding provides new insights into the treatment of osteolytic diseases such as osteoporosis.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Cell & Tissue Engineering
Jeremie D. Oliver, Shihai Jia, Leslie R. Halpern, Emily M. Graham, Emma C. Turner, John S. Colombo, David W. Grainger, Rena N. D'Souza
Summary: Clefts of the lip and/or palate are common orofacial birth defects, with challenges in correcting severe cleft defects through surgery alone. Advances in tissue engineering offer new avenues to stimulate bone formation at the site of surgical correction for palatal clefts, potentially improving treatment outcomes.
TISSUE ENGINEERING PART B-REVIEWS
(2021)
Article
Obstetrics & Gynecology
Goro Kuramoto, Ibrahim A. Hammad, Brett D. Einerson, Amanda A. Allshouse, Michelle Debbink, David W. Grainger, Robert M. Silver, Teruo Okano
Summary: This study demonstrates the feasibility of transplanting human umbilical cord mesenchymal stem cell sheets in a rat model and highlights the potential benefits of reducing uterine fibrosis and scarring. The use of stem cell sheets significantly reduces fibroblast infiltration and uterine scar tissue formation during healing.
AMERICAN JOURNAL OF PERINATOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Kyungsook Kim, Sophia Bou-Ghannam, Sumako Kameishi, Masatoshi Oka, David W. Grainger, Teruo Okano
Summary: The evolution of drug discovery in the early 20th century saw a shift from focusing on symptom control to pursuing radical cures, with cell therapy emerging as a promising new treatment targeting specific tissues and dynamic signaling environments. Cell delivery methods play a crucial role in realizing the therapeutic potential of cell therapy, especially in the context of mesenchymal stem cell (MSC) engraftment and bioactive function. Cell sheet technology is presented as a breakthrough in cell therapy, showcasing therapeutic success in tissue regeneration and discussing its anticipated clinical impacts.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
James M. Anderson, David W. Grainger
Summary: Sung Wan Kim's initial efforts as an independent investigator were focused on improving the understanding of early events in blood/material interactions, leading to the development of blood compatible materials. His studies were supported by NIH grants for over 40 years and contributed to the fields of biomaterials and non-thrombogenic biomaterial research.
JOURNAL OF CONTROLLED RELEASE
(2021)
Editorial Material
Chemistry, Multidisciplinary
David W. Grainger, Wim Hennink, Raymond Schiffelers, You Han Bae
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Computer Science, Information Systems
Alan H. Morris, Brian Stagg, Michael Lanspa, James Orme, Terry P. Clemmer, Lindell K. Weaver, Frank Thomas, Colin K. Grissom, Ellie Hirshberg, Thomas D. East, Carrie Jane Wallace, Michael P. Young, Dean F. Sittig, Antonio Pesenti, Michela Bombino, Eduardo Beck, Katherine A. Sward, Charlene Weir, Shobha S. Phansalkar, Gordon R. Bernard, B. Taylor Thompson, Roy Brower, Jonathon D. Truwit, Jay Steingrub, R. Duncan Hite, Douglas F. Willson, Jerry J. Zimmerman, Vinay M. Nadkarni, Adrienne Randolph, Martha A. Q. Curley, Christopher J. L. Newth, Jacques Lacroix, Michael S. D. Agus, Kang H. Lee, Bennett P. DeBoisblanc, R. Scott Evans, Dean K. Sorenson, Anthony Wong, Michael Boland, David W. Grainger, Willard H. Dere, Alan S. Crandall, Julio C. Facelli, Stanley M. Huff, Peter J. Haug, Ulrike Pielmeier, Stephen E. Rees, Dan S. Karbing, Steen Andreassen, Eddy Fan, Roberta M. Goldring, Kenneth Berger, Beno W. Oppenheimer, E. Wesley Ely, Ognjen Gajic, Brian Pickering, David A. Schoenfeld, Irena Tocino, Russell S. Gonnering, Peter J. Pronovost, Lucy A. Savitz, Didier Dreyfuss, Arthur S. Slutsky, James D. Crapo, Derek Angus, Michael R. Pinsky, Brent James, Donald Berwick
Summary: Clinical decision-making relies on knowledge, expertise, and authority, but unaided clinicians are often affected by cognitive limitations and biases. Electronic health records have the potential to improve healthcare quality by reducing unwarranted clinician decision variation. Providing replicable decision-support tools can personalize patient care, enhance clinical care and research quality, and potentially create a learning healthcare system.
JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION
(2021)
Article
Multidisciplinary Sciences
Tuan Ngoc Nguyen, Andre Rangel, David W. Grainger, Veronique Migonney
Summary: PET fibers and fabrics are commonly used in medical devices, and efforts to enhance their biocompatibility have included functionalizing them with anionic polymers. However, long-term storage of PET fabrics without proper cleaning processes can lead to degradation of spin finish oil, impacting surface cleaning and causing surface degradation. Understanding the effects of storage conditions on these fabrics is important for maintaining their surface functionalization and preventing degradation.
SCIENTIFIC REPORTS
(2021)
Review
Pharmacology & Pharmacy
Ulrich Dirnagl, Georg N. Duda, David W. Grainger, Petra Reinke, Ronenn Roubenoff
Summary: Biomedical research accuracy and relevance are costly problems, with issues in data quality, reporting, methodology, and reproducibility. Preclinical models and clinical data are deficient, leading to inefficient translation from basic research to clinical solutions, resulting in wasted resources and limited new clinical solutions for diseases.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Computer Science, Information Systems
Alan H. Morris, Christopher Horvat, Brian Stagg, David W. Grainger, Michael Lanspa, James Orme, Terry P. Clemmer, Lindell K. Weaver, Frank O. Thomas, Colin K. Grissom, Ellie Hirshberg, Thomas D. East, Carrie Jane Wallace, Michael P. Young, Dean F. Sittig, Mary Suchyta, James E. Pearl, Antinio Pesenti, Michela Bombino, Eduardo Beck, Katherine A. Sward, Charlene Weir, Shobha Phansalkar, Gordon R. Bernard, B. Taylor Thompson, Roy Brower, Jonathon Truwit, Jay Steingrub, R. Duncan Hiten, Douglas F. Willson, Jerry J. Zimmerman, Vinay Nadkarni, Adrienne G. Randolph, Martha A. Q. Curley, Christopher J. L. Newth, Jacques Lacroix, Michael S. D. Agus, Kang Hoe Lee, Bennett P. DeBoisblanc, Frederick Alan Moore, R. Scott Evans, Dean K. Sorenson, Anthony Wong, Michael Boland, Willard H. Dere, Alan Crandall, Julio Facelli, Stanley M. Huff, Peter J. Haug, Ulrike Pielmeier, Stephen E. Rees, Dan S. Karbing, Steen Andreassen, Eddy Fan, Roberta M. Goldring, Kenneth Berger, Beno W. Oppenheimer, E. Wesley Ely, Brian W. Pickering, David A. Schoenfeld, Irena Tocino, Russell S. Gonnering, Peter J. Pronovost, Lucy A. Savitz, Didier Dreyfuss, Arthur S. Slutsky, James D. Crapo, Michael R. Pinsky, Brent James, Donald M. Berwick
Summary: Delivering the best care in various clinical settings is a challenging problem. Evidence-based guidelines currently address only a fraction of the care provided by clinicians, especially in underserved areas. The use of validated clinical decision support systems (eActions) could help overcome cognitive limitations in advanced healthcare environments.
JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION
(2022)
Article
Cell Biology
Sophia Bou-Ghannam, Kyungsook Kim, Makoto Kondo, David W. Grainger, Teruo Okano
Summary: Cell sheet technology is a scaffold-free 3D cell delivery platform that can sustain cell engraftment, survival, and reparative function. Recent evidence shows that cell sheets stimulate mesenchymal stem cells (MSC) to produce paracrine factors. This study introduces a centrifugation strategy to enhance MSC paracrine factor production by stacking and centrifuging cell sheets, resulting in a 3D MSC-delivery platform with boosted therapeutic factor production capacity.
Article
Cell & Tissue Engineering
Masatoshi Oka, Sumako Kameishi, Yun-Kyoung Cho, Sun U. U. Song, David W. W. Grainger, Teruo Okano
Summary: Chronic kidney disease (CKD) is the irreversible loss of nephron function, leading to toxins build-up, inflammation, and fibrosis. Mesenchymal stem/stromal cell (MSC) transplantation is a promising strategy to treat CKD, but concerns remain about kidney decapsulation. In this study, clinically relevant human clonal BMSC (cBMSC) sheets were transplanted onto intact renal capsule, effectively suppressing tubular epithelial cell injury and renal fibrosis in a rat model of ischemia-reperfusion injury (IRI).
TISSUE ENGINEERING PART C-METHODS
(2023)
Article
Cell Biology
Celia M. Dunn, Sumako Kameishi, Yun-Kyoung Cho, Sun U. Song, David W. Grainger, Teruo Okano
Summary: Mesenchymal stromal cells (MSCs) have diverse immunomodulatory functions and hold promise as a treatment for immune-related diseases. In this study, IFN-γ priming and thermoresponsive cultureware were combined to produce transplantable scaffold-free immunosuppressive cell sheets. The use of human clinical-grade single-cell-derived clonal bone marrow-derived MSCs further enhances the clinical relevance of this approach. The results show that MSC sheets produced with IFN-γ priming upregulate expression of immunosuppressive factors and exhibit increased ability to inhibit T-cell proliferation.
Article
Biophysics
Lara J. Gamble, David Radford, David W. Grainger, David G. Castner
Summary: This study characterized the properties of self-assembled monolayers (SAMs) of perfluoroalkanethiols on gold surfaces using XPS, NEXAFS, and ToF-SIMS techniques. It was found that SAMs with longer carbon chains exhibited higher ordering on gold surfaces, while SAMs with shorter carbon chains showed lower ordering.
Editorial Material
Cell Biology
Georg N. Duda, David W. Grainger, Robert E. Guldberg, Gady Goldsobel, Glenn D. Prestwich, Brenna Rauw, Hans-Dieter Volk
Summary: Translational impact assessment is crucial in determining the potential of biomedical research discoveries to be transformed into practical, beneficial products for human health. However, measuring translational success is challenging as it goes beyond commercial achievements and may vary from case to case. Prioritizing societal impact is essential in a globalized society.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Pranothi Mulinti, Dorina Diekjurgen, Kristen Kurtzeborn, Narayanaganesh Balasubramanian, Shane J. Stafslien, David W. Grainger, Amanda E. Brooks
Summary: In this study, a small basic peptide known for its antimicrobial properties was genetically linked to a mechanically robust silk-like peptide. The fusion protein showed both heparin-binding and antimicrobial capabilities, creating a designer silk-like protein with improved hemocompatibility and antimicrobial properties.
BIOENGINEERING-BASEL
(2022)
