Article
Plant Sciences
Sergey Mursalimov, Nobuhiko Ohno, Mami Matsumoto, Sergey Bayborodin, Elena Deineko
Summary: The study using SBF-SEM revealed that intercellular nuclear migration (INM) occurs in 90-100% of tobacco meiocytes, suggesting it is a normal part of tobacco meiosis, yet the reason for such behavior of nuclei remains unclear. The results obtained highlight the potential of SBF-SEM in unveiling unexplored features of plant meiosis.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemical Research Methods
Tong Xin, Yanan Lv, Haoran Chen, Linlin Li, Lijun Shen, Guangcun Shan, Xi Chen, Hua Han
Summary: This article proposes a new method for serial section registration using an unsupervised optical flow network to measure feature similarity and eliminate nonlinear deformation. The optical flow network is also used to estimate and compensate for cumulative registration error, enabling the reconstruction of biological tissue structures. Experimental results demonstrate that this method effectively improves the spatial continuity of serial sections and enhances the accuracy of registration and reconstruction for biological tissue structures.
Article
Materials Science, Multidisciplinary
Fei Yang, Xianping Liu, Peiming Wang, Shunfeng Wang, Ian Robinson, Bo Chen
Summary: Hardened tricalcium silicate (C3S) pastes cured for different times were studied using SBFSEM to analyze their three-dimensional spatial structures and morphological characteristics. Results showed the high reactivity of C3S with water and the complex process of pore network formation. SBFSEM was proven to be applicable in the field of cement-based materials through quantitative analysis compared with traditional methods.
MATERIALS CHARACTERIZATION
(2021)
Article
Cell Biology
David L. Swain, Senila Yasmin, Beatriz Fernandes, Ganimete Lamaj, Yanfeng Su, Haiyan Gong
Summary: Research has found that a decreased number of cellular connections between the inner wall (IW) endothelial cells and the juxtacanalicular connective tissue (JCT) in high-flow areas of the eye is associated with larger giant vacuoles and fewer connections. This suggests that modulating the number of cellular connections can affect the amount of high-flow area around the eye and thereby modulate aqueous humor outflow.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Svenja Appuhn, Sara Siebert, Despoina Myti, Christoph Wrede, David E. Surate Solaligue, David Perez-Bravo, Christina Brandenberger, Julia Schipke, Rory E. Morty, Roman Grothausmann, Christian Muehlfeld
Summary: The study conducted on neonatal mice exposed to different oxygen concentrations revealed that disrupted vascular development may lead to impaired alveolarization, supporting the vascular hypothesis of BPD. Alveolar capillary network in hyperoxia-exposed mice showed rarefaction and altered geometry, with effects on alveolar epithelium and capillary endothelium appearing at different time points. These findings provide valuable insights into the pathogenesis of BPD.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Ophthalmology
David L. Swain, Thuy Duong Le, Senila Yasmin, Beatriz Fernandes, Ganimete Lamaj, Indira Dasgupta, Yanyun Gao, Haiyan Gong
Summary: This study utilized serial block-face scanning electron microscopy to investigate morphological factors associated with GVs with I-pores, revealing a higher presence of GVs with both basal openings and I-pores in high-flow areas, suggesting a potential strategy for glaucoma treatment.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Plant Sciences
Bernd Zechmann, Stefan Moestl, Guenther Zellnig
Summary: Focused ion beam scanning electron microscopy (FIB-SEM) and serial sectioning transmission electron microscopy (ssTEM) are powerful tools for the 3D reconstruction and volumetric extraction of plant cells, and there are differences between the two methods.
Article
Cell Biology
Kiranjit K. Bains, Robert D. Young, Elena Koudouna, Philip N. Lewis, Andrew J. Quantock
Summary: Imaging techniques were used to study the three-dimensional microanatomy and spatiotemporal relationships of cells, extracellular matrix, and vasculature at the corneo-scleral limbus of developing and mature chicken eyes. Results showed the development of structures similar to Palisades of Vogt, associated with characteristics of stem cells, and direct cell-cell associations between corneal epithelial and stromal cells.
Article
Cell Biology
Jose M. Serra Lleti, Anna M. Steyer, Nicole L. Schieber, Beate Neumann, Christian Tischer, Volker Hilsenstein, Mike Holtstrom, David Unrau, Robert Kirmse, John M. Lucocq, Rainer Pepperkok, Yannick Schwab
Summary: This study presents a toolset for adherent cells that enables tracking and finding cells in FIB-SEM, along with automatic acquisition of high-resolution volume datasets. The workflow is completely automated using computer vision techniques, reducing or eliminating operator supervision.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Anatomy & Morphology
Sameh M. Farouk, Samy F. Mahmoud, Haitham Eldoumani, Khaled Shoghy, Nazema S. Abdel-Megeid, Mohamed Abdo
Summary: Through morphometric analysis using scanning electron microscopy, it was found that light cells predominate in all parts of camels' corneas except the central part. The peripheral ventral and nasal parts represent the permanent and endogenous source as well as a proliferative reserve for superficial corneal epithelial cells in dromedary camels.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Chemistry, Physical
Sreejith Raveendran, Anindito Sen, Toru Maekawa, D. Sakthi Kumar
Summary: The study explores the deleterious effects of therapeutic gold nanocages on the subcellular organelles of MCF7 cells, demonstrating significant localization of the nanocarriers within specific organelles leading to their destruction within 96 hours.
Article
Entomology
Josiane Nogueira Mueller, Simone Patricia Carneiro Freitas, Simone Caldas Teves, Jacenir Reis Santos-Mallet, Catarina Macedo Lopes, Teresa Cristina Monte Goncalves
Summary: The study compared the morphological and morphometric aspects of the eggs of Triatoma costalimai and Triatoma jatai, two related species found in Brazil. The results showed significant differences between the eggs of these two species, suggesting that they can be differentiated based on these differences.
JOURNAL OF MEDICAL ENTOMOLOGY
(2023)
Review
Cell Biology
Dominika Czerwinska-Glowka, Katarzyna Krukiewicz
Summary: This paper discusses the significance of SEM in analyzing cell morphology and provides specific guidelines for the analysis of prokaryotic and eukaryotic cell morphology. It emphasizes the importance of careful sample preparation and presents an optimized protocol for observing cell ultrastructure details with a minimum processing effort. Additionally, the paper showcases the wide range of information that can be collected through SEM imaging, making it a convenient screening method for detecting cell pathology.
Article
Plant Sciences
Jan Knoblauch, Rainer Waadt, Asaph B. Cousins, Hans-Henning Kunz
Summary: Researchers have established three microscopy protocols to determine the in situ volume of chloroplasts using chlorophyll fluorescence, embedded CFP markers, and calculations from serial block-face scanning electron microscopy. The obtained data were validated by comparing them with mutant lines. The study also found that the size of guard cell plastids is not governed by the same division machinery as mesophyll plastids.
Article
Materials Science, Multidisciplinary
Zhe Chuan Feng, Hao-Hsiung Lin, Bin Xin, Shi-Jane Tsai, Vishal Saravade, Jeffrey Yiin, Benjamin Klein, Ian T. Ferguson
Summary: The hetero-epitaxy of 3C-SiC on Si-face and C-face 4H-SiC was experimentally investigated, and a corresponding growth model was identified. This study provides valuable insights for band-gap engineering and various applications of SiC.
