Article
Cell & Tissue Engineering
Michael J. Herriges, Maria Yampolskaya, Bibek R. Thapa, Jonathan Lindstrom-Vautrin, Feiya Wang, Jessie Huang, Cheng-Lun Na, Liang Ma, McKenna M. Montminy, Pushpinder Bawa, Carlos Villacorta-Martin, Pankaj Mehta, Darrell N. Kotton
Summary: This study successfully differentiated murine pluripotent stem cells into lung epithelial progenitors and engrafted them into the injured distal lung epithelium. The transplanted cells matured and assumed the phenotypes of specific lung cell types. The study found that the donor-derived cells retained their mature phenotypes, proliferated, and differentiated.
Article
Biophysics
Alice Pievani, Valentina Granata, Giacomo Desantis, Laura Antolini, Sara Ornaghi, Antonio Galleu, Andrea Biondi, Bernhard Gentner, Francesco Dazzi, Marta Serafini
Summary: Monocytes are effective at improving engraftment in HSPC transplantation, particularly in cases of limited cell doses such as CB transplantation and HSC gene therapy. Co-culturing monocytes increases survival and stemness of CB-CD34(+) cells. Soluble factors and direct-cell contact interactions play critical roles in the HSC-monocyte crosstalk.
BONE MARROW TRANSPLANTATION
(2022)
Article
Neurosciences
Ying Wei, Uwimana Alexandre, Xiang Ma
Summary: This study examined four biodegradable hydrogels as supports for hESC-RPE growth. The results showed that only the fibrin support enabled the adhesion and proliferation of ARPE-19 and hESC-RPE cells. In addition, fibrin had a positive effect on the expression of proteins crucial for retinal functions and matrix production. The in vivo investigation revealed good short-term subretinal biocompatibility of fibrin hydrogels.
Article
Cell & Tissue Engineering
Congshan Sun, Suraj Kannan, In Young Choi, HoTae Lim, Hao Zhang, Grace S. Chen, Nancy Zhang, Seong-Hyun Park, Carlo Serra, Shama R. Iyer, Thomas E. Lloyd, Richard M. Lovering, Su Bin Lim, Peter Andersen, Kathryn R. Wagner, Gabsang Lee, Chulan Kwon
Summary: Human pluripotent stem cell (hPSC)-derived myogenic progenitor cell (MPC) transplantation can contribute to muscle fiber regeneration in mice, providing a proof of principle for future hPSC-based cell therapy for muscle disorders.
Article
Multidisciplinary Sciences
Elena Daniele, Lorenzo Bosio, Noor Ahmed Hussain, Barbara Ferrari, Stefano Ferrari, Vanessa Barbaro, Brian McArdle, Nicolo Rassu, Marco Mura, Francesco Parmeggiani, Diego Ponzin
Summary: Recent clinical studies indicate the potential of retinal pigment epithelial (RPE) cell replacement therapy in preserving vision in retinal degenerative diseases. This study aimed to investigate the survival and behavior of human embryonic stem cell-derived RPE (hESC-RPE) cells on a decellularized Descemet's Membrane (DM), which could be a suitable substrate for retinal cell therapy.
Article
Medicine, Research & Experimental
Jamie E. Jeon, Lei Huang, Zhiyuan Zhu, Aaron Wong, Shaf Keshavjee, Mingyao Liu
Summary: This study investigated the effects of ischemia-reperfusion and ex vivo lung perfusion (EVLP) on gene expression in human pulmonary microvascular endothelial cells and epithelial cells. The results showed that both cell types exhibited changes in gene expressions after ischemia-reperfusion or EVLP, with the former showing upregulated pro-inflammatory genes and downregulated cell metabolism pathways, while the latter showed downregulation of cell metabolism without any inflammatory signals.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Mohsen G. Alrashedi, Ahmed Shaker Ali, Osama Abdelhakim Ahmed, Ibrahim M. Ibrahim
Summary: Humanity has been severely affected by the COVID-19 pandemic, resulting in millions of deaths. Azithromycin, an antibiotic, has shown antiviral activity against various viruses, including SARS-CoV-2. The use of nanotechnology to deliver azithromycin directly to the lungs could improve its efficacy and reduce potential side effects.
Article
Immunology
Maite Alvarez, Antonio Pierini, Federico Simonetta, Jeanette Baker, Kristina Maas-Bauer, Toshihito Hirai, Robert S. Negrin
Summary: Allogeneic hematopoietic cell transplantation is an effective treatment option for hematological malignancies, but often results in severe toxicities. Less aggressive alternatives like NMAC and RIC have limitations including increased cancer relapse and limited persistence of donor chimerism. Strategies for accelerated and more durable donor engraftment are still needed.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Physical
Wenhao Zhong, Xinyu Zhang, Yunxin Zeng, Dongjun Lin, Jun Wu
Summary: Lung diseases, including COVID-19 and lung cancers, pose a significant threat to human health, with treatment and diagnosis becoming increasingly challenging. Nanotechnology, as an emerging technology, has shown promise in improving targeted drug delivery in the lungs, offering potential for enhancing therapeutic effects.
Article
Multidisciplinary Sciences
Bhav Harshad Parikh, Paul Blakeley, Kakkad Regha, Zengping Liu, Binxia Yang, Mayuri Bhargava, Daniel Soo Lin Wong, Queenie Shu Woon Tan, Claudine See Wei Wong, Hao Fei Wang, Abdurrahmaan Al-Mubaarak, Chai Chou, Chui Ming Gemmy Cheung, Kah Leong Lim, Veluchamy Amutha Barathi, Walter Hunziker, Gopal Lingam, Tim Xiaoming Hu, Xinyi Su
Summary: Transplantation of stem cell-derived retinal pigment epithelial (RPE) cells is a potential treatment for age-related macular degeneration (AMD). However, there is limited understanding of how the recipient retina regulates the survival and maturation of transplanted RPE cells. In this study, we transplanted stem cell-derived RPE into rabbits and analyzed their gene expression, finding that the transplanted RPE cells retained their identity and showed maturation towards the normal RPE state. Transcription factors FOS, JUND, and MAFF were activated in the transplanted RPE cells and played important roles in regulating gene expression related to supporting host photoreceptor function and promoting cell survival.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Seyoung Kim, Daniel J. Fesenmeier, Sungwan Park, Sandra E. Torregrosa-Allen, Bennett D. Elzey, You-Yeon Won
Summary: This study investigates the clearance pathways and kinetics of amphiphilic poly(styrene-block-ethylene glycol) (PS-PEG) nanoparticles in the lungs. The results demonstrate that PS-PEG nanoparticles have a prolonged retention time in the lungs and are cleared through both mucociliary escalator mechanism and alveolar clearance by macrophages. Additionally, a portion of the nanoparticles translocates directly into the circulation.
Article
Engineering, Biomedical
Maria Maqueda, Jose Luis Mosquera, Jose Garcia-Arumi, Anna Veiga, Anna Duarri
Summary: Decellularized retinas from mouse and pig contain common native ECM components that can guide similar ocular cell adhesion, migration, and organization upon cell repopulation.
Review
Physiology
Chuyang Lin, Xiaolan Zheng, Sha Lin, Yue Zhang, Jinlin Wu, Yifei Li
Summary: Mechanical stress plays a critical role in the development, functional maturation, and pathogenesis of pulmonary tissues. Non-physiological mechanical forces can lead to various pulmonary diseases, with intracellular signaling being involved in this process.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Shinya Watanabe, Ryuhei Hayashi, Yuzuru Sasamoto, Motokazu Tsujikawa, Bruce R. Ksander, Markus H. Frank, Andrew J. Quantock, Natasha Y. Frank, Kohji Nishida
Summary: A subpopulation of cells in hiPSC-derived corneal epithelial cell sheets, positive for ABCB5, demonstrate remarkable holoclone-forming capabilities that can be suppressed by ABCB5 blockade. These cells first emerge in 2D eye-like organoids around six weeks of differentiation and display corneal epithelial characteristics, including sustained expression of ABCB5. This study highlights the translational potential of ABCB5-enriched, hiPSC-derived corneal epithelial cell sheets for vision recovery in stem cell-deficient human eyes and represents the first report of holoclone-forming stem cells identified in an hiPSC-derived organoid.
Article
Endocrinology & Metabolism
Zhen Liang, Dong Sun, Shuaiyao Lu, Zhengjun Lei, Shusen Wang, Zhifeng Luo, Jinqin Zhan, Shuangshuang Wu, Yong Jiang, Zhi Lu, Shicheng Sun, Yingying Shi, Haiting Long, Yanling Wei, Wenhai Yu, Zhihui Wang, Liew Soon Yi, Yun Zhang, Wenyong Sun, Xiaofeng Fang, Yanyan Li, Sufang Lu, Jiayun Lv, Weiguo Sui, Zhongyang Shen, Xiaozhong Peng, Yuanyuan Du, Hongkui Deng
Summary: This study demonstrates the successful implantation of human pluripotent stem cell-derived islets in a new transplantation site in the abdomen, leading to improved glycemic control in diabetic primates. The results suggest the potential of using this transplantation site for human pluripotent stem cell-based therapies for diabetes.
Article
Surgery
Shuhei Tara, Hirotsugu Kurobe, Mark W. Maxfield, Kevin A. Rocco, Tai Yi, Yuji Naito, Christopher K. Breuer, Toshiharu Shinoka
JOURNAL OF VASCULAR SURGERY
(2015)
Article
Multidisciplinary Sciences
Hirotsugu Kurobe, Mark W. Maxfield, Shuhei Tara, Kevin A. Rocco, Paul S. Bagi, Tai Yi, Brooks Udelsman, Zhen W. Zhuang, Muriel Cleary, Yasuko Iwakiri, Christopher K. Breuer, Toshiharu Shinoka
Article
Cell & Tissue Engineering
Hirotsugu Kurobe, Shuhei Tara, Mark W. Maxfield, Kevin A. Rocco, Paul S. Bagi, Tai Yi, Brooks V. Udelsman, Ethan W. Dean, Ramak Khosravi, Heather M. Powell, Toshiharu Shinoka, Christopher K. Breuer
TISSUE ENGINEERING PART C-METHODS
(2015)
Article
Cell & Tissue Engineering
Hirotsugu Kurobe, Mark W. Maxfield, Yuji Naito, Muriel Cleary, Mitchel R. Stacy, Daniel Solomon, Kevin A. Rocco, Shuhei Tara, Avione Y. Lee, Albert J. Sinusas, Edward L. Snyder, Toshiharu Shinoka, Christopher K. Breuer
TISSUE ENGINEERING PART C-METHODS
(2015)
Article
Cell & Tissue Engineering
Liping Zhao, Sumati Sundaram, Andrew V. Le, Angela H. Huang, Jiasheng Zhang, Go Hatachi, Arkadi Beloiartsev, Michael G. Caty, Tai Yi, Katherine Leiby, Ashley Gard, Mehmet H. Kural, Liqiong Gui, Kevin A. Rocco, Amogh Sivarapatna, Elizabeth Calle, Allison Greaney, Luca Urbani, Panagiotis Maghsoudlou, Alan Burns, Paolo DeCoppi, Laura E. Niklason
TISSUE ENGINEERING PART A
(2016)
Article
Anatomy & Morphology
Yuji Naito, Kevin Rocco, Hirotsugu Kurobe, Mark Maxfield, Christopher Breuer, Toshiharu Shinoka
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2014)
Article
Cardiac & Cardiovascular Systems
Shuhei Tara, Hirotsugu Kurobe, Kevin A. Rocco, Mark W. Maxfield, Cameron A. Best, Tai Yi, Yuji Naito, Christopher K. Breuer, Toshiharu Shinoka
Review
Cardiac & Cardiovascular Systems
Shuhei Tara, Kevin A. Rocco, Narutoshi Hibino, Tadahisa Sugiura, Hirotsugu Kurobe, Christopher K. Breuer, Toshiharu Shinoka
CIRCULATION JOURNAL
(2014)
Article
Biophysics
Brooks V. Udelsman, Ramak Khosravi, Kristin S. Miller, Ethan W. Dean, Matthew R. Bersi, Kevin Rocco, Tai Yi, Jay D. Humphrey, Christopher K. Breuer
JOURNAL OF BIOMECHANICS
(2014)
Article
Cardiac & Cardiovascular Systems
Mitchel R. Stacy, Yuji Naito, Mark W. Maxfield, Hirotsugu Kurobe, Shuhei Tara, Chung Chan, Kevin A. Rocco, Toshiharu Shinoka, Albert J. Sinusas, Christopher K. Breuer
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2014)
Review
Cell & Tissue Engineering
Kevin A. Rocco, Mark W. Maxfield, Cameron A. Best, Ethan W. Dean, Christopher K. Breuer
TISSUE ENGINEERING PART B-REVIEWS
(2014)
Review
Biochemistry & Molecular Biology
Tomoshi Tsuchiya, Amogh Sivarapatna, Kevin Rocco, Atsushi Nanashima, Takeshi Nagayasu, Laura E. Niklason
Article
Biochemistry & Molecular Biology
Micha Sam Brickman Raredon, Kevin A. Rocco, Ciprian P. Gheorghe, Amogh Sivarapatna, Mahboobe Ghaedi, Jenna L. Balestrini, Thomas L. Raredon, Elizabeth A. Calle, Laura E. Niklason
BIORESEARCH OPEN ACCESS
(2016)
Article
Cardiac & Cardiovascular Systems
Tatsuo Motoki, Hirotsugu Kurobe, Yoichiro Hirata, Taisuke Nakayama, Hajime Kinoshita, Kevin A. Rocco, Hitoshi Sogabe, Takaki Hori, Masataka Sata, Tetsuya Kitagawa
GENERAL THORACIC AND CARDIOVASCULAR SURGERY
(2015)