期刊
ACTA PHYSIOLOGIAE PLANTARUM
卷 35, 期 1, 页码 253-262出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11738-012-1071-2
关键词
24-epibrassinolide; Ca(NO3)(2) stress; AsA-GSH cycle; Cucumber roots; Polyamines
资金
- National Basic Research Program of China (973 Program) [2009CB119000]
- National Natural Science Foundation of China [30900995, 31071831]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- China earmarked fund for Modern Agro-industry Technology Research Systems [CARS-25-C-03]
The effects of 24-epibrassinolide (EBL) applied by spraying the leaves of Cucumis sativus L (cv. Jinyou No.4) on the roots of ascorbate-glutathione (AsA-GSH) cycle and levels and forms of polyamines were investigated under Ca(NO3)(2) stress. Ca(NO3)(2) stress caused significant decreases in ascorbate acid (AsA) and glutathione (GSH) levels and the activities of antioxidant enzymes involved in the AsA-GSH cycle, including ascorbate peroxidase (APX, EC 1.11.1.11), glutathione reductase (GR, EC 1.6.4.2), monodehydroascorbate reductase (MDAR, EC 1.6.5.4) and dehydroascorbate reductase (DHAR, EC 1.6.5.4). In contrast, exogenous EBL in NB combination increased AsA and GSH levels through enhancing activities of APX, GR, MDAR and DHAR. This alleviated the declines of AsA/DHA and GSH/GSSG ratios, which could maintain cellular homeostasis. In addition, EBL application reduced the accumulation of putrescine (Put) induced by excess Ca(NO3)(2), and promoted the conversion of Put into spermidine (Spd) and/or spermine (Spm) able to bind to cell membrane structures. These results showed that EBL could alleviate the damage from oxidative stress by up-regulating the capacity of the AsA-GSH cycle and altering polyamine levels and forms.
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