Editorial Material
Plant Sciences
Wim Van den Ende
Summary: Researchers have discovered a novel fructan 1-exohydrolase (1-FEH) in onions, which belongs to the vacuolar invertase subgroup and suggests that plant fructan exohydrolases could have originated from different ancestral invertases. This finding will contribute to further understanding the evolution and functions of plant FEHs in fructan-accumulating and non-fructan-accumulating plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biotechnology & Applied Microbiology
Suling Zhang, Zhen Zhang, Xun Sun, Zhiqiang Liu, Min Ma, Jinbu Fan, Weiqi Luo, Libin Wang, Shaoling Zhang
Summary: The 23 invertase genes identified in pear genome, including different types such as vacuolar, cell wall, and alkaline/neutral invertases, exhibit tissue-specific expression profiles and can be affected by postharvest treatments. Changes in invertase activity and gene expression profiles during pear development may be associated with visual/inner quality alterations, with PbrvacInv1 potentially playing a role in sucrose decomposition.
Article
Biochemistry & Molecular Biology
Lin Chen, Fenghua Zheng, Zili Feng, Yue Li, Muxuan Ma, Guoping Wang, Hongbo Zhao
Summary: The research found that the expression of the CsVI2 gene and the activity of vacuolar invertase in cucumber seedlings were significantly induced by drought stress. In addition, drought stress led to changes in the expression of certain related genes and alterations in the activities of sucrose metabolism enzymes. Transgenic cucumber seedlings overexpressing CsVI2 exhibited enhanced vacuolar invertase activity and drought tolerance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Satoshi Oku, Keiji Ueno, Yukiko Sawazaki, Tomoo Maeda, Yutaka Jitsuyama, Takashi Suzuki, Shuichi Onodera, Kaien Fujino, Hanako Shimura
Summary: A novel type of fructan exohydrolase (FEH) was discovered in onion, which shares amino acid similarities with vacuolar invertases, unlike previously known plant FEHs, and is localized in vacuoles like fructosyltransferases.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Bolaji Babajide Salam, Francois Barbier, Raz Danieli, Paula Teper-Bamnolker, Carmit Ziv, Lukas Spichal, Kalaivani Aruchamy, Yula Shnaider, Diana Leibman, Felix Shaya, Mira Carmeli-Weissberg, Amit Gal-On, Jiming Jiang, Naomi Ori, Christine Beveridge, Dani Eshel
Summary: The study reveals that sucrose induces bud outgrowth in potato stems by promoting the accumulation of cytokinin (CK) and the activity of vacuolar invertase (VInv). The effects of sucrose can be suppressed by CK synthesis and perception inhibitors, while CK can induce bud outgrowth even in the absence of sucrose. Overall, the results identify a branching-promoting module where sugar-induced lateral bud outgrowth is partially promoted by CK-mediated VInv activity.
Article
Plant Sciences
Jiaoyan Lv, Baizhi Chen, Changkai Ma, Kaikai Qiao, Shuli Fan, Qifeng Ma
Summary: This study identified a total of 92 AINV genes in five cotton species, revealing that GhAINVs were significantly conserved in structure and expression. Experimental results suggested the potential role of GhAINVs in stress response.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Kai Zhang, Zhengdan Wu, Xuli Wu, Haohao Han, Xisan Ju, Yonghai Fan, Chaobin Yang, Daobin Tang, Qinghe Cao, Jichun Wang, Changwen Lv
Summary: The Ibβfruct2 gene in sweet potato regulates starch content, with Ibβfruct2-1 acting as a negative regulator and Ibβfruct2-2 and Ibβfruct2-3 being functionally deficient. These findings are important for understanding the regulatory and functional divergence of duplicated genes in sweet potato.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Md Dulal Ali Mollah, Xian Zhang, Li Zhao, Xiaohan Jiang, Collins O. Ogutu, Qian Peng, Mohammad A. A. Belal, Qiurui Yang, Yaming Cai, Elsayed Nishawy, Sylvia Cherono, Lu Wang, Yuepeng Han
Summary: This study identified two vacuolar invertase inhibitor genes, PpINHa and PpINH3, involved in sugar accumulation in peach fruits. Overexpression of PpINH3 increased sugar content, while overexpression of PpINHa had the opposite effect. Surprisingly, both PpINH3 and PpINHa had no physical interaction with vacuolar invertase (VIN), and the expression of PpVIN genes in ripe fruits was negligible. The results suggest that the regulatory role of PpINHa and PpINH3 in sugar accumulation is likely due to their impact on genes related to fruit sugar metabolism and transport.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Anna M. Hubmann, Alexandra Roth, Stephan Monschein
Summary: The activities of acid invertase and vacuolar invertase were investigated during bud dormancy release and bud break in deciduous fruit trees. Acid invertase activity increased sharply during bud break, indicating an increasing demand for carbohydrates. Vacuolar invertase activity showed a similar pattern but decreased after cold spells, potentially protecting the young leaf tissues from cold stress.
Article
Plant Sciences
Aneela Yasmeen, Sana Shakoor, Saira Azam, Allah Bakhsh, Naila Shahid, Ayesha Latif, Ahmad Ali Shahid, Tayyab Husnain, Abdul Qayyum Rao
Summary: The knockdown of the VInv gene in potato using CRISPR/Cas9 resulted in a decrease in post-harvest cold-storage sugars formation and sweetening in potatoes. The transformation efficiency of potatoes was 11.7%, and the overall editing efficacy was 25.6%. The edited potato plants showed a significant reduction in the mRNA expression of the VInv gene and a fivefold reduction in the percentage of reducing sugars in tubers after cold storage. The best lines for cold storage analysis were B4 and T12.
Article
Plant Sciences
Xuli Wu, Zhengdan Wu, Xisan Ju, Yonghai Fan, Chaobin Yang, Yonghui Han, Wanxia Chen, Daobin Tang, Changwen Lv, Qinghe Cao, Jichun Wang, Kai Zhang
Summary: This study identified three invertase inhibitors as potential interaction partners of Ibl3FRUCT2 and confirmed IbInvInh2 as a novel VIF in sweet potato. IbInvInh2 can regulate plant starch content by inhibiting the activity of Ibl3FRUCT2, providing a foundation for using VIFs to improve the starch properties of crops.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Zhong-Rui Xu, Shen-Wen Cai, Ting-Ting You, Zhi-Ting Xiong
Summary: This study reveals that the promoter activity and expression of EhvINVMP, a vacuolar invertase gene, are induced by glucose and Cu stress, while those of EhvINVNMP, an allele from a non-metalliferous population, are not affected. The glucose induction of EhvINVMP provides a positive feed-forward mechanism to amplify its induction by Cu stress, explaining the different regulation of EhvINVNMP and EhvINVMP under Cu stress.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Hongwei Guo, Min Zhou, Guoyan Zhang, Li He, Caihong Yan, Min Wan, Jianjun Hu, Wei He, Deying Zeng, Bo Zhu, Zixian Zeng
Summary: Polyploid plants have advantages in agronomically important traits. This study developed homozygous autotetraploid and diploid lines from a heterozygous diploid potato, and investigated the differences in phenotypes, transcription, and histone modifications between the two ploidies. The tetraploid lines produced larger but fewer tubers, and displayed ploidy-related differential gene expression. H3K27ac was found to be enriched in genic regions upon whole-genome doubling (WGD), but was not associated with the differential gene expression between two ploidies. The tetraploid lines also showed better resistance to cold-induced sweetening (CIS), which may be regulated through the expression of CIS-related key genes and levels of H3K4me3 in cold-stored tubers.
HORTICULTURE RESEARCH
(2023)
Article
Plant Sciences
Amelie Morin, Fadia Kadi, Benoit Porcheron, Cecile Vriet, Laurence Maurousset, Remi Lemoine, Nathalie Pourtau, Joan Doidy
Summary: Invertases are important enzymes in carbon metabolism, and their gene families are limited in legumes. This study identified invertase genes and proposed a new nomenclature for plant invertases. It also revealed the regulatory roles of invertases in seed development and water stress in peas. Vacuolar and cell wall invertases were found to play crucial roles in maintaining embryo survival in drought conditions.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Plant Sciences
Paula Teper-Bamnolker, Marina Roitman, Omri Katar, Noam Peleg, Kalaivani Aruchamy, Shlomit Suher, Adi Doron-Faigenboim, Diana Leibman, Ayelet Omid, Eduard Belausov, Mariette Andersson, Niklas Olsson, Ann-Sofie Faelt, Hanne Volpin, Per Hofvander, Amit Gal-On, Dani Eshel
Summary: To cope with cold stress, plants develop antioxidation strategies and osmoprotection by sugars. In potato tubers, exposure to cold stress induces starch degradation and sucrose synthesis. Vacuolar acid invertase (VInv) activity is a significant part of the cold-induced sweetening (CIS) response, increasing osmoprotection.
Article
Plant Sciences
Su-fen Chen, Dong-mei Yin, Ke Liang, Cecile Raynaud, Di-an Ni
ACTA PHYSIOLOGIAE PLANTARUM
(2015)
Article
Biochemistry & Molecular Biology
Su-Fen Chen, Ke Liang, Dong-Mei Yin, Di-An Ni, Zhi-Guo Zhang, Yong-Ling Ruan
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2016)
Article
Plant Sciences
Di An Ni, Rosangela Sozzani, Sophie Blanchet, Severine Domenichini, Christophe Reuzeau, Rino Cella, Catherine Bergounioux, Cecile Raynaud
Article
Biochemistry & Molecular Biology
Yu-xin Tan, Jin Liang, Zheng Zhang, Shu-qi Dong, Jun-feng Cao, Qiao-ping Qin, Zhi-guo Zhang, Di-an Ni
Summary: This study measured and compared the glyoxalase I activity of different daylily varieties and introduced a putative glyoxalase I gene from daylily into tobacco. The results showed a positive correlation between glyoxalase I activity and salt tolerance in daylily, and the transgenic tobacco exhibited tolerance to abiotic stress. These findings could contribute to the selection of salt-tolerant daylily and provide a molecular tool for enhancing the biotic tolerance of ornamental plants.
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Xiang Li, Mingsen Yu, Pablo Bolanos-Villegas, Jun Zhang, Di'an Ni, Hong Ma, Yingxiang Wang
Summary: The study revealed the crucial role of FANCM in shaping the distribution of meiotic Class-I crossovers in plants, with evolutionary divergent roles observed between Arabidopsis and lettuce in meiotic bivalent formation.
