期刊
ACTA PHARMACOLOGICA SINICA
卷 29, 期 2, 页码 169-176出版社
ACTA PHARMACOLOGICA SINICA
DOI: 10.1111/j.1745-7254.2008.00710.x
关键词
mesenchymal stem cell; salvianolic acid B; cell survival; spinal cord injuries
Aim: Stem cells hold great promise for brain and spinal cord injuries (SCI), but cell survival following transplantation to adult central nervous system has been poor. Salvianolic acid B (Sal B) has been shown to improve functional recovery in brain-injured rats. The present study was designed to determine whether Sal B could improve transplanted mesenchymal stem cell (MSC) survival in SCI rats. Methods: SCI rats were treated with Sal B. The Basso-Beatie-Bresnahan (BBB) scale was used to test the functional recovery. Sal B was used to protect MSC from being damaged by TNF-alpha in vitro. Bromodeoxyuridine-labeled MSC were transplanted into SCI rats with Sal B intraperitoneal injection, simultaneously. MSC were examined, and the functional recovery of the SCI rats was tested. Results: Sal B treatment significantly reduced the lesion area from 0.26 +/- 0.05 mm(2) to 0.15 +/- 0.03 mm(2) (P < 0.01) and remarkably raised the BBB scores on d 28, post-injury, from 7.3 +/- 0.9 to 10.5 +/- 1.3 (P < 0.05), compared with the phosphate-buffered saline (PBS) control group. MSC were protected from the damage of TNF-alpha by Sal B. The number of surviving MSC in the MSC plus Sal B groups were 1143.3 +/- 195.6 and 764.0 +/- 81.3 on d 7 and 28, post-transplantation, more than those in the MSC group, which was 569.3 +/- 72.3 and 237.0 +/- 61.3, respectively (P < 0.05). Rats with MSC transplanted and Sal B injected obtained higher BBB scores than those with MSC transplanted alone (P < 0.05) and PBS (P < 0.01). Conclusion: Sal B provides neuroprotection to SCI and promotes the survival of MSC in vitro and after cell transplantation to the injured spinal cord in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据