Article
Biochemical Research Methods
Filip Ottosson, Francesco Russo, Anna Abrahamsson, Nadia MacSween, Julie Courraud, Zaki Krag Nielsen, David M. Hougaard, Arieh S. Cohen, Madeleine Ernst
Summary: There are over 2.5 million neonatal dried blood spots (DBS) stored at the Danish National Biobank, offering great potential for metabolomics research. However, little is known about the long-term stability of metabolites in these samples. In this study, we analyzed 200 neonatal DBS samples collected over 10 years and found that the majority of the metabolome remained stable during storage. However, some lipid-related metabolites showed decreasing trends, while a few metabolites were strongly influenced by storage.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Microbiology
Jean H. H. Kim, Veronica Robles, Kristin E. D. Weimer, Lisa H. M. Gehtland, Katerina S. S. Kucera
Summary: The implementation of cCMV screening in public health programs is hindered by challenges such as limited specimen availability and an insufficiently sensitive DBS-based assay. Efforts have been made to improve the currently available DBS-based method in order to increase its feasibility in newborn screening programs. Evaluation: 7 out of 10.
MICROBIOLOGY SPECTRUM
(2023)
Article
Medical Laboratory Technology
Shou-Fang Qu, Hao-Ran Tao, Liu-Ji Qin, Wen-Xin Zhang, Shan Han, Shen-Yan Zhang, Jie Huang
Summary: The DBS reference materials developed in this study can be used to evaluate the testing capabilities of different laboratories and provide consistency and standardization for multi-center analysis in the screening of neonatal inherited metabolic disorders.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Lenka Rysava, Milos Dvorak, Pavel Kuban
Summary: This study introduces a simple and convenient concept of automated blood sampling and analysis, allowing for accurate collection of blood samples and automated processing and analysis in a laboratory setting. Using warfarin blood concentration determination as an example, the concept demonstrates excellent analytical performance and wide applicability to various types of blood compounds.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Nora Grecso, Anita Zadori, Akos Barath, Zsolt Galla, Gabor Racz, Csaba Bereczki, Peter Monostori
Summary: This study compared five different QC preparation approaches for CAH diagnostics in dried blood spots, finding that four techniques produced similar results in terms of reproducibility and concentration ranges, while the third technique showed significantly lower concentrations in some cases. These findings have implications for the in-house preparation of QC samples in laboratory developed tests for CAH.
Article
Medical Laboratory Technology
Nick Flynn, Stuart J. Moat, Sarah L. Hogg
Summary: A computer vision algorithm was developed and validated to measure DBS diameter and identify incorrectly applied blood. CV demonstrated excellent agreement with digital calipers and could accurately assess DBS size and quality. CV can aid in reducing the number of unsuitable NBS specimens and minimizing the impact of DBS diameter on analyte concentrations.
CLINICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Agnieszka Niemiec
Summary: DBS as a new method of sample collection is simple, rapid, and does not require trained personnel to collect samples, playing an important role in newborn screening and toxicology, with a broad development potential in the future.
Article
Genetics & Heredity
Chinwe O. Okeke, Reuben I. Chianumba, Hezekiah Isa, Samuel Asala, Obiageli E. Nnodu
Summary: Using dried blood spots on HemoTypeSC for sickle cell disease testing is as accurate as the standard point-of-care test. The use of dried blood spots can improve the efficiency and cost-effectiveness of mass newborn screening for SCD.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Justyna Spiewak, Ivan Doykov, Apostolos Papandreou, Jenny Haellqvist, Philippa Mills, Peter T. Clayton, Paul Gissen, Kevin Mills, Wendy E. Heywood
Summary: Dried blood spot (DBS) biomarkers are convenient for monitoring specific lysosomal storage diseases (LSDs), but may also have relevance for other LSDs. A study was conducted to determine the specificity and utility of glycosphingolipidoses biomarkers in a DBS cohort of patients with various LSDs. The findings indicated that DBS glucosylceramide isoform profiling increased the specificity for the detection of Niemann-Pick disease type C (NPC), thereby improving diagnostic accuracy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Abhishek Jain, Montana Morris, Elizabeth Z. Lin, Sajid A. Khan, Xiaomei Ma, Nicole C. Deziel, Krystal J. Godri Pollitt, Caroline H. Johnson
Summary: Dried blood spot (DBS) metabolomics has many applications but faces challenges in accurate metabolite quantification due to the hematocrit effect and unknown blood-spotting volumes. This study compared five normalization methods and found that Hb normalization outperformed others in terms of intragroup variation, differential metabolic analysis, and classification accuracy. The study also demonstrated a correlation between specific gravity (SG) and Hb in adults and proposed an equation to use SG as a surrogate for Hb normalization. This comparative study provides a robust methodological platform for future environmental epidemiological studies.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Immunology
Aroa Silgado, Lidia Gual-Gonzalez, Adrian Sanchez-Montalva, Ines Oliveira-Souto, Lidia Goterris, Nuria Serre-Delcor, Juliana Esperalba, Jordi Gomez-I-Prat, Candela Fernandez-Naval, Israel Molina, Tomas Pumarola, Elena Sulleiro
Summary: This study evaluated the use of dried blood sampling and a rapid diagnostic test for the detection of antibodies against T. cruzi in community-based screening. The results showed a higher sensitivity in dried blood elutes using E-CLIA compared to ELISA. The Trypanosoma Detect (TM) test was easier to use but had a lower sensitivity. A sequential strategy of using the rapid test first and confirming with DBS-ECLIA for negative results is proposed for community Chagas disease screening programs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Rima Jeske, Uta Merle, Barbara Mueller, Tim Waterboer, Julia Butt
Summary: This study tested the compatibility of dried blood spot (DBS) samples with a bead-based multiplex serology assay for detecting SARS-CoV-2 antibodies. The results showed a high correlation between DBS samples and serum samples, and the classification of SARS-CoV-2 seropositivity remained consistent when adjusting the cutoffs. This technology enables powerful sero-epidemiological studies in resource-poor settings.
Article
Oncology
Chuan Zhou, Li Li, Xiaoxia Feng, Dandan Wang, Yuanyuan Liu, Jingjing Li, Hong Song, Baisui Feng
Summary: The study found that GAL-3 in serum has a higher sensitivity and specificity for diagnosing early and severe neonatal necrotising enterocolitis. The results also suggest that GAL-3 level serves as an independent prognostic measure, indicating poor prognosis in NEC.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2021)
Review
Chemistry, Multidisciplinary
Valentina Iacuzzi, Bianca Posocco, Martina Zanchetta, Sara Gagno, Ariana Soledad Poetto, Michela Guardascione, Giuseppe Toffoli
Summary: This review critically investigates the DBS-to-plasma concentration conversion methods proposed and applied in the last decade for quantitative bioanalysis of anticancer drugs in DBS matrix, highlighting the need to address challenges in comparing drug concentrations obtained from DBS with those obtained from reference matrix to ensure treatment efficacy.
PHARMACEUTICAL RESEARCH
(2021)
Article
Chemistry, Analytical
Chih-Ning Cheng, Yu-Fong Peng, Ju-Yu Chen, Guan-Yuan Chen, Te- Weng, Ching-Hua Kuo
Summary: This study proposed an effective DBS preparation protocol to comprehensively evaluate the hematocrit (Hct) effect for accurate quantification of DBS samples. An LC-MS/MS method was used to quantify the target drugs in DBS samples, and the results showed the validity of the proposed protocol. This new protocol eliminated common pitfalls in studying the Hct effect and provided a comprehensive strategy for further DBS studies.
ANALYTICA CHIMICA ACTA
(2023)
Article
Pediatrics
Katrine Kure Pollstergaard, Rikke Wiingreen, Rasmus Gregersen, Rene Mathiasen, Bo Molholm Hansen, Gorm Greisen
Summary: The study shows that very low birth weight has negative effects on the socioeconomic outcomes of young adults, although the relative disadvantages are smaller compared to childhood.
Article
Pediatrics
Janne Weis, Sanne Allermann Beck, Andreas Jensen, Anne Brodsgaard, Jos M. M. Latour, Gorm Greisen
Summary: In this study, a Danish version of the EMPATHIC-N questionnaire was translated and validated, and its psychometric properties were assessed. The shorter version of the questionnaire showed better model fit and reliability.
Review
Pediatrics
Mohamed El-Dib, Nicholas S. J. Abend, Topun Austin, Geraldine Boylan, Valerie Chock, M. Roberta Cilio, Gorm Greisen, Lena Hellstroem-Westas, Petra Lemmers, Adelina Pellicer, Ronit Pressler, Arnold Sansevere, Tammy Tsuchida, Sampsa Vanhatalo, Courtney J. Wusthoff
Summary: The development of neonatal neurocritical care in the past decade has shown significant progress in neuromonitoring and neuroprotection. Commonly used brain monitoring tools in the neonatal intensive care unit (NICU) include amplitude integrated EEG (aEEG), full multichannel continuous EEG (cEEG), and near-infrared spectroscopy (NIRS). However, there is no consensus on the consistent and efficient use of these modalities in common NICU scenarios. This review summarizes current evidence for the best utilization of neuromonitoring modalities in neonates with encephalopathy or possible seizures.
PEDIATRIC RESEARCH
(2023)
Review
Pediatrics
Mohamed El-Dib, Nicholas S. Abend, Topun Austin, Geraldine Boylan, Valerie Chock, M. Roberta Cilio, Gorm Greisen, Lena Hellstrom-Westas, Petra Lemmers, Adelina Pellicer, Ronit M. Pressler, Arnold Sansevere, Eniko Szakmar, Tammy Tsuchida, Sampsa Vanhatalo, Courtney J. Wusthoff
Summary: Neonatal intensive care has shifted focus from cardiorespiratory care to a holistic approach that emphasizes brain health. The most commonly used tools in the neonatal intensive care unit (NICU) to monitor brain function and physiology are amplitude-integrated EEG, full multichannel continuous EEG, and near-infrared spectroscopy. Each of these tools has unique characteristics and functions, but there is no consensus on the optimal approach to neuromonitoring in the NICU. This review examines current evidence to guide the use of these neuromonitoring tools for neuroprotective care in extremely premature infants and critically ill neonates.
PEDIATRIC RESEARCH
(2023)
Article
Clinical Neurology
Signe V. V. Pedersen, Rikke Wiingreen, Bo M. Hansen, Gorm Greisen, Mads L. Larsen, Christina E. Hoei-Hansen
Summary: This study investigates the performance of children with cerebral palsy (CP) in the Danish school system and identifies factors associated with school performance. The results show that children with CP are less likely to complete elementary school compared to those without CP and have lower overall grades. The study concludes that the complex needs of children with CP may present challenges in an inclusive school environment.
DEVELOPMENTAL MEDICINE AND CHILD NEUROLOGY
(2023)
Article
Obstetrics & Gynecology
Louise Laub Asserhoj, Ikram Mizrak, Gerda Ferja Heldarskard, Tine Dalsgaard Clausen, Eva R. Hoffmann, Gorm Greisen, Katharina M. Main, Per Lav Madsen, Rikke Beck Jensen, Anja Pinborg
Summary: The study found that BMI in childhood does not differ between children conceived after frozen embryo transfer (FET), fresh embryo transfer (fresh-ET), or natural conception (NC). Previous studies have shown that high childhood BMI is strongly associated with obesity, cardiometabolic disease, and mortality in adulthood. Children conceived after FET have a higher risk of being born large for gestational age (LGA), which is associated with an increased risk of childhood obesity. The study suggests that ART-induced epigenetic variations may influence fetal size at birth as well as BMI and health later in life.
HUMAN REPRODUCTION
(2023)
Article
Multidisciplinary Sciences
Andreas Jensen, Gorm Greisen, Thomas Hjuler, Lone Graff Stensballe
Summary: This study aimed to assess the utilization of surgery in Danish children aged 0-5 years from 1999 to 2018. The results showed that the overall incidence of surgical procedures did not increase during this period. It was also found that boys underwent more surgery than girls, and the rate of surgery decreased in public hospitals but increased in private specialist practices among children with severe chronic disease.
Article
Pediatrics
Rikke Wiingreen, Gorm Greisen, Ellen C. L. Lokkegaard, Christian Torp-Pedersen, Kathrine K. Sorensen, Mikkel P. Andersen, Bo M. Hansen
Summary: This study investigated the association between gestational age and grade point averages in language and mathematics at the end of lower secondary education. The findings showed that decreasing gestational age was associated with lower grades in mathematics, but there were only minor differences in grades in language. Children with shorter gestational age were also more likely to have mathematical difficulties.
Article
Pediatrics
Emma Louise Malchau Carlsen, Kia Hee Schultz Dungu, Anna Lewis, Nadja Hawwa Vissing, Lise Aunsholt, Simon Trautner, Hristo Stanchev, Gholamreza Krog Dayani, Anne-Janet L. Pedersen, Mia Bjerager, Maria De Salas, Kristian Vestergaard, Pernille Pedersen, Niels Frimodt-Moller, Gorm Greisen, Bo Molholm Hansen, Ulrikka Nygaard
Summary: This study evaluated the implementation of switch therapy from intravenous to oral antibiotic treatment in neonates with early-onset infection. The results showed that switch therapy was safe and effective in relieving the treatment burden for neonates, caregivers, and healthcare systems.
ARCHIVES OF DISEASE IN CHILDHOOD-FETAL AND NEONATAL EDITION
(2023)
Article
Pediatrics
Charlotte Carina Holm-Hansen, Stine Lund, Tine Bruhn Skytte, Jil Molenaar, Christina Nadia Steensgaard, Ulfat Amour Mohd, Said Mzee, Said Mouhammed Ali, Jesper Kjaergaard, Gorm Greisen, Jette Led Sorensen, Anja Poulsen
Summary: The aim of this study was to assess risk factors for neonatal mortality, quality of neonatal resuscitation, and identify areas for improvement. The study found significant deviations from guidelines in the quality of care for neonatal resuscitation, despite 87.5% of health workers being trained in this area. The videos provided direct evidence of gaps in care and highlighted the need for future education in effective positive pressure ventilation.
PEDIATRIC RESEARCH
(2023)
Article
Pediatrics
Anne Mette Plomgaard, Nathan Stevenson, James A. Roberts, Tue Hvass Petersen, Sampsa Vanhatalo, Gorm Greisen, SafeBoosC-II study group
Summary: Automated computational measures of EEG in extremely preterm infants were found to be predictive of neurodevelopmental impairment at 2 years of age. The association between EEG measures and neurodevelopment in childhood was confirmed in this study, and the measure appears to be robust to differences in electrodes, amplifiers, and filters.
PEDIATRIC RESEARCH
(2023)
Article
Medicine, Research & Experimental
Maria Linander Vestager, Mathias Luehr Hansen, Marie Isabel Rasmussen, Gitte Holst Hahn, Simon Hyttel-Sorensen, Adelina Pellicer, Anne Marie Heuchan, Cornelia Hagmann, Eugene Dempsey, Gabriel Dimitriou, Gerhard Pichler, Gunnar Naulaers, Hans Fuchs, Jakub Tkaczyk, Jonathan Mintzer, Monica Fumagalli, Saudamini Nesargi, Siv Fredly, Tomasz Szczapa, Christian Gluud, Janus Christian Jakobsen, Gorm Greisen
Summary: The SafeBoosC-IIIv trial aims to evaluate the benefits and harms of cerebral oximetry added to usual care versus usual care in mechanically ventilated newborns. This randomized clinical trial, involving 3000 participants from multiple countries, will assess outcomes such as mortality rate and neurodevelopmental disabilities. The findings from this study will provide important guidance for neonatal intensive care and treatment of brain injuries.
Article
Medicine, Research & Experimental
Marie Isabel Rasmussen, Mathias Luhr Hansen, Adelina Pellicer, Christian Gluud, Eugene Dempsey, Jonathan Mintzer, Simon Hyttel-Sorensen, Anne Marie Heuchan, Cornelia Hagmann, Ebru Ergenekon, Gabriel Dimitriou, Gerhard Pichler, Gunnar Naulaers, Guoqiang Cheng, Jakub Tkaczyk, Hans Fuchs, Monica Fumagalli, Saudamini Nesargi, Siv Fredly, Tomasz Szczapa, Anne Mette Plomgaard, Bo Molholm Hansen, Janus Christian Jakobsen, Gorm Greisen
Summary: The SafeBoosC-III follow-up study aims to assess mortality, neurodevelopmental disability, or any harm in trial participants at 2 years of corrected age. The lack of funding for trial-specific assessment local costs is a challenge that needs to be addressed.
Article
Medicine, General & Internal
Liv Borch-Johnsen, Caroline Gren, Stine Lund, Fredrik Folke, Morten Schroder, Marianne Sjolin Frederiksen, Freddy Lippert, Annette Kjaer Ersboll, Gorm Greisen, Dina Cortes
Summary: This study assessed the effectiveness and safety of video tutorials in caring for acutely ill children. The results showed that video tutorials significantly increased caregivers' self-efficacy, reduced the need for telephone triage, and resulted in fewer hospital assessments for children.
Article
Pediatrics
David G. Sweet, Virgilio P. Carnielli, Gorm Greisen, Mikko Hallman, Katrin Klebermass-Schrehof, Eren Ozek, Arjan te Pas, Richard Plavka, Charles C. Roehr, Ola D. Saugstad, Umberto Simeoni, Christian P. Speer, Maximo Vento, Gerry H. A. Visser, Henry L. Halliday
Summary: The sixth version of European Guidelines for the Management of RDS has been developed by a panel of experienced European neonatologists and an expert perinatal obstetrician based on available literature up to end of 2022. These updated guidelines aim to optimize outcomes for babies with RDS through various measures such as prediction of risk of preterm delivery, appropriate maternal transfer to a perinatal center, and evidence-based lung-protective management. The guidelines also address the importance of general care for infants with RDS, including cardiovascular support and judicious use of antibiotics. The guidelines have received endorsement from the European Society for Paediatric Research (ESPR) and the Union of European Neonatal and Perinatal Societies (UENPS). Rating: 7 out of 10.
